##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937024_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176673 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.985753340917967 33.0 31.0 34.0 30.0 34.0 2 31.962393800976947 33.0 31.0 34.0 30.0 34.0 3 31.942237919772687 33.0 31.0 34.0 30.0 34.0 4 35.53162622472025 37.0 35.0 37.0 33.0 37.0 5 35.555053686754626 37.0 35.0 37.0 33.0 37.0 6 35.71808935151381 37.0 35.0 37.0 35.0 37.0 7 35.72694752452271 37.0 35.0 37.0 35.0 37.0 8 35.71656676458768 37.0 35.0 37.0 35.0 37.0 9 37.298760987813644 39.0 37.0 39.0 34.0 39.0 10-11 37.18316041500399 39.0 37.0 39.0 34.0 39.0 12-13 37.105865072761546 39.0 37.0 39.0 33.5 39.0 14-15 37.90784103966084 40.0 37.5 41.0 33.0 41.0 16-17 37.938541825859076 40.0 37.5 41.0 33.0 41.0 18-19 38.06420335874752 40.0 37.5 41.0 33.5 41.0 20-21 38.06482597793664 40.0 37.5 41.0 34.0 41.0 22-23 37.91258426584707 40.0 37.0 41.0 33.5 41.0 24-25 37.752500382061775 40.0 37.0 41.0 33.0 41.0 26-27 37.50512245787415 39.5 36.5 41.0 33.0 41.0 28-29 37.330740973436804 39.5 36.0 41.0 33.0 41.0 30-31 36.960138221459985 39.0 35.0 41.0 33.0 41.0 32-33 36.46840207615199 38.0 35.0 41.0 32.0 41.0 34-35 36.1434231603018 38.0 35.0 40.0 31.0 41.0 36-37 35.72206562406254 38.0 35.0 40.0 30.5 41.0 38-39 35.32196204287016 38.0 35.0 40.0 30.0 41.0 40-41 34.99130314196283 38.0 35.0 40.0 29.5 41.0 42-43 34.66439410662637 38.0 35.0 40.0 26.5 41.0 44-45 34.177050256688915 37.5 35.0 40.0 23.5 41.0 46-47 33.414848335625706 37.0 33.5 40.0 16.5 41.0 48-49 32.77749854250508 37.0 33.0 40.0 4.5 41.0 50-51 31.827010918476507 36.0 32.0 39.0 2.0 40.5 52-53 31.9047732251108 36.5 32.5 39.5 2.0 40.0 54-55 32.22731826594895 37.0 33.0 40.0 2.0 41.0 56-57 32.00999586807266 36.5 33.0 40.0 2.0 41.0 58-59 31.790316007539353 36.0 32.5 40.0 2.0 41.0 60-61 31.272899650767236 35.5 31.5 39.5 2.0 41.0 62-63 30.888514374013006 35.0 31.0 39.0 2.0 41.0 64-65 30.363479422435802 35.0 30.5 39.0 2.0 40.5 66-67 29.80675315413221 35.0 29.5 38.5 2.0 40.0 68-69 29.40872402687451 35.0 29.0 37.5 2.0 39.0 70-71 28.76490748444867 35.0 28.5 37.0 2.0 39.0 72-73 28.18632728260685 35.0 26.5 36.0 2.0 38.5 74-75 27.806962580586735 34.5 26.0 36.0 2.0 37.0 76-77 27.278183423613115 34.0 26.0 35.0 2.0 37.0 78-79 26.768176235191568 34.0 25.0 35.0 2.0 36.0 80-81 26.309501168826024 34.0 23.0 35.0 2.0 36.0 82-83 25.892668942056794 33.5 20.5 35.0 2.0 35.5 84-85 25.503008382718356 33.0 19.0 35.0 2.0 35.0 86-87 25.154375597855925 33.0 14.0 35.0 2.0 35.0 88-89 24.59837666196872 32.5 4.5 35.0 2.0 35.0 90-91 24.009520413419143 32.0 2.0 35.0 2.0 35.0 92-93 23.647252268314908 32.0 2.0 35.0 2.0 35.0 94-95 23.285445427428073 32.0 2.0 35.0 2.0 35.0 96-97 22.694310958663745 31.0 2.0 35.0 2.0 35.0 98-99 22.149173897539523 31.0 2.0 35.0 2.0 35.0 100-101 20.434919314213264 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31022036048438295 1101 2 0.5612034660544474 1101 3 0.27823296185333746 1101 4 0.26147932753152503 1101 5 0.30792875841488865 1101 6 0.1784700852165102 1101 7 0.32607719682645353 1101 8 0.32719139221004667 1101 9 0.49636817228204677 1101 10-11 0.41825958928914986 1101 12-13 0.5694526255801904 1101 14-15 0.6275082196858506 1101 16-17 0.6970793871021641 1101 18-19 0.7351161231027916 1101 20-21 0.5306356070309661 1101 22-23 0.7243191281614898 1101 24-25 0.8609630697831534 1101 26-27 0.913190498614874 1101 28-29 0.5759536863123103 1101 30-31 0.8653178174094336 1101 32-33 1.0235264477444943 1101 34-35 1.1101620231441416 1101 36-37 0.892208500778743 1101 38-39 1.159105235974657 1101 40-41 1.0970168528322617 1101 42-43 1.1834853792851803 1101 44-45 1.5396455584797408 1101 46-47 1.5533452707889381 1101 48-49 2.244828222573979 1101 50-51 2.006987430294398 1101 52-53 1.725747071707893 1101 54-55 1.8247980203308352 1101 56-57 1.7918243930744389 1101 58-59 2.034564488621811 1101 60-61 2.072584422584807 1101 62-63 2.190590062141851 1101 64-65 2.0844449835353203 1101 66-67 1.765960597774928 1101 68-69 1.6943056735945206 1101 70-71 1.9405210480106767 1101 72-73 2.0271085502549653 1101 74-75 2.1234283386310615 1101 76-77 2.361090043037237 1101 78-79 2.5144834434408487 1101 80-81 2.4944788851340363 1101 82-83 2.4476658437509506 1101 84-85 2.4533726753382865 1101 86-87 2.4360795441178844 1101 88-89 2.3896429146477622 1101 90-91 2.041423401422044 1101 92-93 2.0573370648740656 1101 94-95 2.154914748781117 1101 96-97 2.42186285111179 1101 98-99 2.4325208085331234 1101 100-101 2.7185407827373957 1102 1 0.047016061715158486 1102 2 0.3620756110306722 1102 3 0.12951556303764633 1102 4 0.3453100932795792 1102 5 0.15370683802790808 1102 6 0.06736691713797427 1102 7 0.05170896577952533 1102 8 -0.1996818727337768 1102 9 -0.041049468398988154 1102 10-11 0.10258957463425844 1102 12-13 0.13815787155605364 1102 14-15 0.209968812315509 1102 16-17 0.4719182428288917 1102 18-19 0.1457629639601663 1102 20-21 0.3307392636060058 1102 22-23 0.2914218673162665 1102 24-25 0.16369984165528706 1102 26-27 0.21590344138929396 1102 28-29 -0.07600446979211029 1102 30-31 0.17255233147455584 1102 32-33 0.41984008632856273 1102 34-35 0.24409942410444785 1102 36-37 0.040365915798226126 1102 38-39 -0.3436470248109913 1102 40-41 0.22253066775166275 1102 42-43 0.37368923721610514 1102 44-45 0.738910430815352 1102 46-47 0.318795462017448 1102 48-49 0.14602086891442667 1102 50-51 -0.1155656192343848 1102 52-53 -0.08527626889331685 1102 54-55 -0.03667152012501873 1102 56-57 0.23816126502878276 1102 58-59 -0.09320713330572516 1102 60-61 0.24362968595591283 1102 62-63 -0.016061830484236594 1102 64-65 0.20872564264816162 1102 66-67 0.07003775639331522 1102 68-69 0.42071784549762725 1102 70-71 0.7806575887405636 1102 72-73 0.5604395006785268 1102 74-75 0.6886009208712345 1102 76-77 0.5276792651363422 1102 78-79 0.42502305335851887 1102 80-81 0.698062781429801 1102 82-83 0.7637886112623598 1102 84-85 0.9965807867631469 1102 86-87 0.745208471451047 1102 88-89 1.4816887618317587 1102 90-91 1.1568455934607442 1102 92-93 1.6174340493017496 1102 94-95 1.582492163562307 1102 96-97 1.5643608554248551 1102 98-99 2.0037936397873715 1102 100-101 1.822423734209437 1103 1 0.041934663990208065 1103 2 0.1925503743301782 1103 3 -0.012245897353334811 1103 4 -0.20609648665366365 1103 5 0.10454124985271562 1103 6 0.0934959274233762 1103 7 0.2392847439962651 1103 8 0.14462709804477925 1103 9 0.043413442846834016 1103 10-11 0.0201837439687651 1103 12-13 -0.12979390284623804 1103 14-15 0.13621361852761993 1103 16-17 0.3909768900600099 1103 18-19 0.17059778447647744 1103 20-21 0.1954964327361779 1103 22-23 0.29533849424904446 1103 24-25 0.21475222228330892 1103 26-27 0.46970086663280597 1103 28-29 0.4480129677121454 1103 30-31 0.38330772960819814 1103 32-33 0.24393245060893065 1103 34-35 0.6059276374414182 1103 36-37 0.3305981888041529 1103 38-39 0.8573678924289823 1103 40-41 0.7217132847396073 1103 42-43 0.8117997201532248 1103 44-45 0.6648214722975396 1103 46-47 1.0873262160532775 1103 48-49 1.6104238387393721 1103 50-51 1.385217706152165 1103 52-53 1.587600238156611 1103 54-55 1.758785192632967 1103 56-57 1.7766055126958342 1103 58-59 1.8408164345711242 1103 60-61 1.9774352850295465 1103 62-63 2.218424921099441 1103 64-65 1.6759077147141 1103 66-67 1.22847383023025 1103 68-69 1.4905632238778495 1103 70-71 1.6563813132824912 1103 72-73 1.6326007975929073 1103 74-75 1.5733567276963818 1103 76-77 1.5634874525895128 1103 78-79 1.7367388765313265 1103 80-81 1.4542646750337802 1103 82-83 1.4732838772524133 1103 84-85 1.469600335840184 1103 86-87 1.4066007471815851 1103 88-89 1.6032676733500502 1103 90-91 1.8364791334103359 1103 92-93 1.9680164346978408 1103 94-95 1.9767841055281146 1103 96-97 1.9754091491378176 1103 98-99 1.9707236854245913 1103 100-101 2.046911166945627 1104 1 -0.11217646635425993 1104 2 -0.19240721611468814 1104 3 -0.0717231346737428 1104 4 -0.13375790261350318 1104 5 -0.14358061270570488 1104 6 -0.19466401718455728 1104 7 -0.3574090417056581 1104 8 -0.2878811242159074 1104 9 -0.3267613883804401 1104 10-11 -0.408654753521283 1104 12-13 -0.5118520645218538 1104 14-15 -0.841698792758514 1104 16-17 -0.20507642480929889 1104 18-19 -0.37132709002586495 1104 20-21 -0.5133057137066146 1104 22-23 -0.7335259682747548 1104 24-25 -0.793599863574407 1104 26-27 -0.7007163049095197 1104 28-29 -0.5956861840967562 1104 30-31 -0.1379749430592767 1104 32-33 -0.315181276238917 1104 34-35 -0.445050378830004 1104 36-37 -0.3173246084954826 1104 38-39 -0.0017631444413623854 1104 40-41 0.22681974137800154 1104 42-43 0.2786423720486795 1104 44-45 -0.025952285708342515 1104 46-47 -0.15368988226764912 1104 48-49 -0.17567621319069815 1104 50-51 -0.28156436066452173 1104 52-53 0.035107594916176765 1104 54-55 0.6825431985183776 1104 56-57 0.36777740121928915 1104 58-59 0.18640237883284883 1104 60-61 0.2704074158933132 1104 62-63 -0.44316413896633833 1104 64-65 0.08024387535110833 1104 66-67 0.26313715029256457 1104 68-69 0.19710641238399518 1104 70-71 0.0529972038831481 1104 72-73 0.41794786354409297 1104 74-75 0.5527664559290315 1104 76-77 0.44371940689105216 1104 78-79 0.6196625393320865 1104 80-81 0.4687877839138217 1104 82-83 0.5846825270706582 1104 84-85 0.6493562645228934 1104 86-87 0.23846849860965946 1104 88-89 0.3721269428670979 1104 90-91 -0.09140731284204406 1104 92-93 -0.007238888134079247 1104 94-95 0.11297936258194596 1104 96-97 0.34866516437063666 1104 98-99 0.35297225734773363 1104 100-101 0.47699598605589344 1105 1 0.36671325467176175 1105 2 0.4322885337448845 1105 3 -0.008626336321377437 1105 4 0.03240948177143821 1105 5 0.28536527757700725 1105 6 0.3645009331992668 1105 7 0.26995474784686024 1105 8 0.042066451144513906 1105 9 0.03080273818761725 1105 10-11 0.13241901543584333 1105 12-13 0.24505207328005696 1105 14-15 0.33532798125384033 1105 16-17 0.2772302593322422 1105 18-19 0.03185115374871117 1105 20-21 0.15323613238962253 1105 22-23 0.39285485928562025 1105 24-25 0.055000585779652056 1105 26-27 0.07804703528894663 1105 28-29 -0.33513104460975995 1105 30-31 -0.5488062480583338 1105 32-33 -0.8494445228219547 1105 34-35 -0.8262055932093304 1105 36-37 -0.4607668477765188 1105 38-39 -0.5480325696426362 1105 40-41 -0.7083983825688236 1105 42-43 -0.5776111385298677 1105 44-45 -0.49400602096391566 1105 46-47 -0.16227909777036587 1105 48-49 -0.06787804805308184 1105 50-51 -0.162625959616026 1105 52-53 -0.13255763631392625 1105 54-55 -0.1667827704013014 1105 56-57 -0.030883898162851864 1105 58-59 0.05850030057035127 1105 60-61 0.5183478764812186 1105 62-63 0.8949747863285538 1105 64-65 0.7242712110560312 1105 66-67 0.36035677208623795 1105 68-69 0.09258478722016861 1105 70-71 -0.10671071598204662 1105 72-73 -0.13176192862965763 1105 74-75 0.19871290790431573 1105 76-77 0.10001036364072391 1105 78-79 -0.31244287163963946 1105 80-81 -0.3687426411002228 1105 82-83 0.017536861464964915 1105 84-85 0.1323650643731078 1105 86-87 0.06378211029286618 1105 88-89 0.04086875151716285 1105 90-91 -0.2905039239635556 1105 92-93 -0.187518798263266 1105 94-95 -0.25148890178801864 1105 96-97 -0.491697127335037 1105 98-99 -0.6545169768780674 1105 100-101 -0.23279618769434407 1106 1 -0.04435623641581188 1106 2 -0.10943195191773114 1106 3 0.04278156861274951 1106 4 -0.003111055557411646 1106 5 0.26159880896425136 1106 6 0.24773523958005228 1106 7 -0.02202406899633047 1106 8 0.39514059219333575 1106 9 0.47558444834002955 1106 10-11 0.26943141533251236 1106 12-13 -0.024742553431380543 1106 14-15 0.29548039665038317 1106 16-17 0.4318414709054821 1106 18-19 0.4570319855751208 1106 20-21 0.11429270691816384 1106 22-23 -0.4696483698541343 1106 24-25 -0.2226731419392891 1106 26-27 -0.028030205734047797 1106 28-29 -0.018836805395089584 1106 30-31 -0.4030428794197505 1106 32-33 -0.37960468815390414 1106 34-35 -0.21990231240333458 1106 36-37 -0.2692465687788399 1106 38-39 0.06062798318608742 1106 40-41 0.0696951333185396 1106 42-43 0.47670299218571444 1106 44-45 0.40721459386429615 1106 46-47 0.49791852558277583 1106 48-49 0.5175649899216026 1106 50-51 0.9974102067492545 1106 52-53 1.2014645801675954 1106 54-55 0.8194610942331586 1106 56-57 1.0463571248286279 1106 58-59 0.7361352786122026 1106 60-61 1.317237052404753 1106 62-63 1.1553726163466358 1106 64-65 1.0853536187026336 1106 66-67 1.3075280456589589 1106 68-69 0.7232357817952106 1106 70-71 0.9053662667097662 1106 72-73 0.9974682444522323 1106 74-75 1.4176485834403536 1106 76-77 1.2975691339843074 1106 78-79 1.7728387093471838 1106 80-81 1.6884777153968074 1106 82-83 1.4570550738131978 1106 84-85 1.4304792467549206 1106 86-87 1.5766986693215408 1106 88-89 1.5557873771952835 1106 90-91 1.6403601938354626 1106 92-93 1.9360603802435783 1106 94-95 2.041878445150754 1106 96-97 1.9764958460568565 1106 98-99 2.2747002796447937 1106 100-101 2.190414868880186 1107 1 0.20824986931762268 1107 2 0.2601696862058738 1107 3 0.37282624471103887 1107 4 0.23544069425643244 1107 5 0.40856455728889784 1107 6 0.022214018433359684 1107 7 0.11284190270880856 1107 8 -0.10709321272103267 1107 9 -0.21754660154827832 1107 10-11 0.19934578614412857 1107 12-13 0.3213575954889478 1107 14-15 0.3154450280725669 1107 16-17 0.5461378223574513 1107 18-19 0.16472930500921734 1107 20-21 0.5587733472755758 1107 22-23 0.9647336107862188 1107 24-25 0.4235480047472322 1107 26-27 0.6847397123597787 1107 28-29 0.5161594715967155 1107 30-31 0.778174544258583 1107 32-33 0.5091239045489999 1107 34-35 0.164265595266059 1107 36-37 -0.01966540720029286 1107 38-39 3.6178810045583987E-4 1107 40-41 0.5052870916208505 1107 42-43 0.7122297978446852 1107 44-45 0.9461200294562531 1107 46-47 1.340947192745844 1107 48-49 0.8144748931233892 1107 50-51 0.4786555529890144 1107 52-53 0.7698283180678231 1107 54-55 0.6690515633321859 1107 56-57 0.6551155015970487 1107 58-59 0.4936743701981996 1107 60-61 0.2777266279818207 1107 62-63 -0.07666386913363255 1107 64-65 0.047425472760711074 1107 66-67 0.5687901454355782 1107 68-69 -0.022436221183191662 1107 70-71 -0.35971191646223843 1107 72-73 0.15192654676049955 1107 74-75 0.223772931913917 1107 76-77 0.4293170863297995 1107 78-79 -0.33615235543316047 1107 80-81 -0.3911015846776529 1107 82-83 -0.07212400288077347 1107 84-85 -0.04485582398223187 1107 86-87 -0.01004742151985738 1107 88-89 0.22941917168523318 1107 90-91 -0.4730519431712388 1107 92-93 -0.17116225564621956 1107 94-95 0.32864315103310915 1107 96-97 -0.12202425818737339 1107 98-99 0.19977473980860694 1107 100-101 0.3270027318734847 1108 1 0.25890346408886344 1108 2 0.26915661431045024 1108 3 0.18491774797901073 1108 4 0.29998317791656604 1108 5 0.03193057036079239 1108 6 0.11453427987127185 1108 7 0.18882229486566615 1108 8 0.23277606832471776 1108 9 0.47281287557590446 1108 10-11 0.26388826980426217 1108 12-13 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72-73 -2.315460781305056 2316 74-75 -2.707343544527401 2316 76-77 -2.6293784067928208 2316 78-79 -2.236686449667868 2316 80-81 -2.6767688366530713 2316 82-83 -2.556595030097629 2316 84-85 -2.357227787693091 2316 86-87 -2.7255873688420706 2316 88-89 -2.9251937929035883 2316 90-91 -3.3299622446052446 2316 92-93 -3.2122801953594227 2316 94-95 -4.0339922016154155 2316 96-97 -4.136089959094605 2316 98-99 -4.336185166541991 2316 100-101 -4.167967271053058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 416.0 3 139.0 4 93.0 5 103.0 6 134.0 7 174.0 8 225.0 9 464.0 10 690.0 11 986.0 12 1228.0 13 1530.0 14 1797.0 15 2352.0 16 3013.0 17 4185.0 18 3582.0 19 2043.0 20 2006.0 21 2092.0 22 2126.0 23 2177.0 24 2386.0 25 2434.0 26 2628.0 27 2929.0 28 3305.0 29 3821.0 30 4583.0 31 5543.0 32 6935.0 33 8866.0 34 12345.0 35 21121.0 36 22805.0 37 26596.0 38 16703.0 39 2117.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.75564112001359 17.553863140907673 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18.333748429239353 44-45 15.349978490728372 28.939875925464715 36.05746371725496 19.652681866551948 46-47 16.755627755859827 28.19142011331926 34.28020761989936 20.772744510921555 48-49 17.004344196182203 26.89099888211238 35.592692694109154 20.51196422759626 50-51 17.279254649244205 28.129519410647625 37.992624604839 16.598601335269162 52-53 15.753380070813794 29.406969865929284 36.18494230458988 18.654707758667048 54-55 13.629287457047592 26.400092839472627 39.01012742783712 20.960492275642657 56-57 17.620487042760587 28.974628944061408 33.10266164000498 20.302222373173024 58-59 17.68230382792047 26.820915587631784 34.72921531168188 20.767565272765868 60-61 16.9708153146014 24.776416781760137 38.44046460026716 19.812303303371294 62-63 19.968412829599103 25.481732652529804 35.715579607594506 18.834274910276587 64-65 18.143482604524266 27.54861478636663 37.591944280138904 16.715958328970196 66-67 16.85500764309574 26.00917171488422 36.560323840797146 20.575496801222894 68-69 15.205123776463358 28.932518255718804 36.882356438839444 18.980001528978388 70-71 17.663117353874036 27.889102854553695 32.759401903035794 21.688377888536476 72-73 15.94708644302368 25.60856533958243 35.29296891583246 23.151379301561427 74-75 16.099850264231268 28.547166767150973 35.461336186521514 19.891646782096245 76-77 17.914617886593124 24.884291695328358 35.3782918481907 21.822798569887816 78-79 16.316951647605027 24.818253878382972 38.50781338466765 20.356981089344355 80-81 17.63686263180702 27.62941374729872 35.50937058342043 19.224353037473836 82-83 15.673629767610397 26.160744869678947 36.15584739117265 22.009777971538007 84-85 16.526701028301645 24.93256458374021 34.802052640666176 23.73868174729197 86-87 18.028893110213374 25.62274759153307 35.62011476843896 20.7282445298146 88-89 19.316009887785526 23.54946582739415 34.37959242616016 22.754931858660164 90-91 16.78765777448088 23.513706348352464 37.22175348392101 22.47688239324564 92-93 17.26652925321072 27.421062764728532 36.10359238034837 19.208815601712384 94-95 16.802056031383916 27.94606314144839 34.19624004392842 21.05564078323927 96-97 14.249927820519353 27.005598863243947 34.164671116319354 24.579802199917346 98-99 17.54503190880932 27.033584941986554 31.900032276879 23.521350872325126 100-101 19.57113441772335 24.98166091239439 33.84361785589867 21.60358681398359 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2347.0 1 1631.0 2 1007.0 3 1011.0 4 736.5 5 679.0 6 1259.0 7 2585.0 8 4167.0 9 4361.0 10 2848.0 11 1623.0 12 1384.5 13 1323.5 14 1257.0 15 1131.5 16 984.5 17 954.0 18 952.0 19 936.5 20 957.5 21 984.5 22 1013.5 23 1062.5 24 1138.5 25 1278.5 26 1451.0 27 1555.5 28 1618.5 29 1681.0 30 1813.0 31 1930.5 32 1993.5 33 2134.0 34 2292.0 35 2447.0 36 2631.0 37 2796.5 38 2695.5 39 2439.0 40 2268.5 41 2132.0 42 2006.0 43 1968.5 44 2059.0 45 2435.0 46 4367.5 47 10475.0 48 28795.5 49 27761.5 50 8878.5 51 3546.0 52 1809.0 53 1295.0 54 1122.5 55 1029.0 56 977.5 57 876.0 58 798.0 59 796.5 60 808.0 61 798.0 62 676.0 63 394.5 64 186.0 65 132.0 66 101.0 67 71.0 68 47.0 69 32.0 70 24.0 71 18.0 72 15.5 73 17.5 74 14.5 75 8.0 76 5.0 77 4.0 78 4.0 79 2.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03848918623671982 2 0.03282901179014337 3 0.03113095945617044 4 0.052073604908503275 5 0.09395889581316896 6 0.11376950637618652 7 0.0549036921317915 8 0.08660066903261958 9 0.0701861631375479 10-11 0.0752803201394667 12-13 0.07216722419384965 14-15 0.06622404102494439 16-17 0.05122457874151681 18-19 0.0599978491337103 20-21 0.0549036921317915 22-23 0.03481007284644513 24-25 0.00792424422520702 26-27 0.003113095945617044 28-29 0.006226191891234088 30-31 0.004245130834932332 32-33 0.005943183168905266 34-35 0.007641235502878198 36-37 0.005943183168905266 38-39 0.003396104667945866 40-41 0.00792424422520702 42-43 0.00396212211260351 44-45 0.002830087223288222 46-47 0.002264069778630577 48-49 2.8300872232882215E-4 50-51 0.0025470785009593998 52-53 0.007075218058220554 54-55 0.013584418671783465 56-57 0.008490261669864664 58-59 0.005943183168905266 60-61 0.002830087223288222 62-63 0.010754331448495243 64-65 0.001981061056301755 66-67 0.024338750120278706 68-69 0.047828474073570944 70-71 0.06395997124631381 72-73 0.004245130834932332 74-75 0.016131497172742863 76-77 0.02462175884260753 78-79 0.02999892456685515 80-81 0.07726138119576846 82-83 0.029149898399868684 84-85 0.012735392504796997 86-87 0.031979985623156904 88-89 0.05179059618617446 90-91 0.04301732579398097 92-93 0.04358334323863861 94-95 0.012452383782468176 96-97 0.01698052333972933 98-99 0.04301732579398097 100-101 0.07782739864042611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 176673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.01919365154834 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3510010970927 27.852020399268707 2 8.600178277564455 5.679419039694803 3 2.7530170049369174 2.7270720483605304 4 1.223944048272079 1.6165458219422322 5 0.6222572682391663 1.0273216620536245 6 0.4731212287438289 0.9373248883530589 7 0.3479840921557872 0.8043107888585126 8 0.22284695556774547 0.5886581424439501 9 0.143993417443774 0.4279091881611791 >10 0.9908118486012067 6.161665902543116 >50 0.0977098189797038 2.116339225574932 >100 0.09256719692814043 6.023557646046651 >500 0.039426769061985736 9.240234784036044 >1k 0.039426769061985736 31.360196521256782 >5k 0.0017142073505211192 3.437423941405874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6073 3.437423941405874 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4285 2.425384750358006 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4176 2.3636888488903227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4103 2.3223695754303146 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3529 1.997475562196827 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3449 1.9521941666242153 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3363 1.9035166663836578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3180 1.7999354740113092 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3125 1.7688045145551385 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3097 1.7529560261047246 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2661 1.5061724202339917 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2400 1.3584418671783465 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2330 1.3188206460523113 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2240 1.2678790760331233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2034 1.1512794824336485 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1835 1.0386420109467773 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1486 0.8411019227612594 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1405 0.7952545097439904 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1180 0.6679005846960203 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1157 0.6548821834688945 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1148 0.6497880264669756 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1142 0.6463919217990299 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 0.5948843343351842 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1029 0.582431950552716 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 926 0.5241321537529786 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 924 0.5230001188636634 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 902 0.5105477350811952 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 880 0.49809535129872706 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 874 0.4946992466307812 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 809 0.4579081127280343 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 804 0.4550780255047461 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 770 0.4358334323863861 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 762 0.431305292829125 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 0.42281503115926033 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 695 0.39338212403706285 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 671 0.37979770536527935 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 670 0.3792316879206217 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 669 0.37866567047596406 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 651 0.3684773564721265 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 628 0.35545895524500065 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 602 0.3407425016839019 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 593 0.3356483446819831 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 567 0.3209318911208843 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.3203658736762267 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 565 0.31979985623156904 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 530 0.2999892456685515 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 520 0.29432907122197505 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 476 0.26942430365703873 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 430 0.24338750120278707 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 430 0.24338750120278707 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 422 0.23885936164552593 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 361 0.20433229752140963 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 352 0.19923814051949082 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 341 0.1930119486282567 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 337 0.19074787884962616 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 331 0.18735177418168028 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 309 0.1748993903992121 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 294 0.16640912872934743 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 267 0.15112665772359105 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 263 0.14886258794496046 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 260 0.14716453561098752 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 254 0.14376843094304168 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 228 0.1290519773819429 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 217 0.12282578549070883 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 205 0.11603357615481709 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.10697729704029478 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 186 0.10527924470632186 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.10131712259371835 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.660174446576443E-4 0.0 18-19 0.0 0.0 0.0 0.003113095945617044 0.0 20-21 0.0 0.0 0.0 0.005094157001918799 0.0 22-23 0.0 0.0 0.0 0.009905305281508776 0.0 24-25 0.0 0.0 0.0 0.017263532062058155 0.0 26-27 0.0 0.0 0.0 0.027734854788224572 0.0 28-29 0.0 0.0 0.0 0.043866351960967435 0.0 30-31 0.0 0.0 0.0 0.07103518930453437 0.0 32-33 0.0 0.0 0.0 0.08858173008892134 0.0 34-35 0.0 0.0 0.0 0.11009039298591182 0.0 36-37 0.0 0.0 0.0 0.13641020416249228 0.0 38-39 0.0 0.0 0.0 0.16273001533907275 0.0 40-41 0.0 0.0 0.0 0.22272786447278303 0.0 42-43 0.0 0.0 0.0 0.33508232723732545 0.0 44-45 0.0 0.0 0.0 0.43838051088734553 0.0 46-47 0.0 0.0 0.0 0.6851641167580784 0.0 48-49 0.0 0.0 0.0 0.8962886236153798 0.0 50-51 0.0 0.0 0.0 1.0315667928885568 0.0 52-53 0.0 0.0 0.0 1.1719391191636526 0.0 54-55 0.0 0.0 0.0 1.3199526809416264 0.0 56-57 0.0 0.0 0.0 1.6476767813984026 0.0 58-59 0.0 0.0 0.0 2.102188789458491 0.0 60-61 0.0 0.0 0.0 2.5997181233125604 0.0 62-63 0.0 0.0 0.0 3.040928721423194 0.0 64-65 0.0 0.0 0.0 3.477045162531909 0.0 66-67 0.0 0.0 0.0 4.049854816525445 0.0 68-69 0.0 0.0 0.0 4.638795967691724 0.0 70-71 0.0 0.0 0.0 5.055101798237422 0.0 72-73 0.0 0.0 0.0 5.47112462006079 0.0 74-75 0.0 0.0 0.0 5.96129572713431 0.0 76-77 0.0 0.0 0.0 6.407883490969192 0.0 78-79 0.0 0.0 0.0 6.8773949613127074 0.0 80-81 0.0 0.0 0.0 7.521805822055436 0.0 82-83 0.0 0.0 0.0 8.218007278984338 0.0 84-85 0.0 0.0 0.0 9.52211147147555 0.0 86-87 0.0 0.0 0.0 10.78121727711648 0.0 88-89 0.0 0.0 0.0 11.792407441997362 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGGG 15 6.580578E-4 95.10148 3 ATTAGTT 20 0.0020624066 71.32611 5 GGGAACT 40 7.4968502E-6 59.404747 7 GGGAGAT 25 0.005004325 57.028557 7 TGGGTGA 45 1.5062269E-5 52.80422 6 ATGGGTG 65 4.353933E-8 51.20849 5 CATGGGT 85 1.2551027E-10 50.34784 4 GGGATGT 60 1.4799425E-6 47.5238 7 ACCTGGG 75 6.838758E-6 38.040592 3 TACCTGG 75 6.838758E-6 38.040592 2 CCTGTGG 60 0.003921422 31.700493 3 GATTGAG 30 0.0039540236 31.655628 28-29 ATGGGGA 110 2.6936796E-6 30.25956 5 ATGGGGT 80 4.4504908E-4 29.719212 5 GGGTGAA 80 4.465297E-4 29.702374 7 GACTCCC 65 0.005824661 29.237131 9 TGGGAGG 85 6.366873E-4 27.955175 6 ATGGGAG 220 7.4578566E-11 25.936766 5 TGGGAAC 110 9.4000854E-5 25.92207 6 TGGGATA 95 0.0012188805 25.012526 6 >>END_MODULE