##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937024_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176673 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.916795435635326 31.0 31.0 34.0 30.0 34.0 2 31.96230889836025 31.0 31.0 34.0 30.0 34.0 3 32.05899033808222 33.0 31.0 34.0 30.0 34.0 4 35.71488569277705 37.0 35.0 37.0 33.0 37.0 5 35.596599367192496 37.0 35.0 37.0 33.0 37.0 6 35.829453283750205 37.0 35.0 37.0 35.0 37.0 7 35.7829209896249 37.0 35.0 37.0 35.0 37.0 8 35.80832951271558 37.0 35.0 37.0 35.0 37.0 9 37.36625290791462 39.0 37.0 39.0 34.0 39.0 10-11 37.27770513887238 39.0 37.0 39.0 33.5 39.0 12-13 37.18846965863488 39.0 37.0 39.0 33.0 39.0 14-15 37.856019312515215 39.0 37.0 41.0 33.0 41.0 16-17 38.00242821483758 39.0 37.0 41.0 33.5 41.0 18-19 38.30455700644694 40.0 37.0 41.0 34.0 41.0 20-21 38.3490912589926 40.0 37.0 41.0 34.0 41.0 22-23 38.27791739541413 40.0 37.0 41.0 34.0 41.0 24-25 38.05816678270024 40.0 37.0 41.0 33.5 41.0 26-27 37.533403519496474 39.5 35.5 41.0 32.5 41.0 28-29 37.34811487890057 39.0 36.0 41.0 33.0 41.0 30-31 37.25045422899933 39.0 35.0 41.0 33.0 41.0 32-33 36.89092843841447 39.0 35.0 41.0 33.0 41.0 34-35 36.587427054501816 38.0 35.0 40.0 32.0 41.0 36-37 36.22848709197218 38.0 35.0 40.0 31.0 41.0 38-39 35.855209907569346 38.0 35.0 40.0 30.5 41.0 40-41 35.378159084863 38.0 35.0 40.0 29.5 41.0 42-43 35.13320654542573 38.0 35.0 40.0 28.5 41.0 44-45 34.74590627883151 38.0 35.0 40.0 26.0 41.0 46-47 34.065558970527476 38.0 34.0 40.0 22.5 41.0 48-49 33.41983494931314 37.0 33.0 40.0 19.0 41.0 50-51 32.99102013324051 37.0 33.0 40.0 13.5 41.0 52-53 32.58662896990485 36.5 33.0 40.0 7.5 41.0 54-55 32.293338540693824 36.0 33.0 40.0 2.0 41.0 56-57 32.01600131316047 35.5 32.0 39.5 2.0 41.0 58-59 31.740285725606064 36.0 31.5 39.0 2.0 40.0 60-61 31.04997368018882 35.0 31.0 39.0 2.0 40.0 62-63 30.589034544044647 35.0 30.0 38.0 2.0 40.0 64-65 30.061458174140927 35.0 29.0 38.0 2.0 39.5 66-67 29.703372897952715 35.0 29.0 37.0 2.0 39.0 68-69 29.18303872125339 34.5 29.0 36.5 2.0 39.0 70-71 28.544180491642752 34.0 27.5 36.0 2.0 38.0 72-73 28.01286557651707 34.0 27.0 35.0 2.0 37.0 74-75 27.549458604314182 33.0 26.0 35.0 2.0 37.0 76-77 26.064842958459977 31.5 25.0 34.0 2.0 35.0 78-79 26.6729409700407 33.0 25.5 35.0 2.0 36.0 80-81 26.51482116678836 33.0 25.0 35.0 2.0 35.5 82-83 26.163774317524464 33.0 24.5 35.0 2.0 35.0 84-85 25.76877904377013 33.0 23.0 35.0 2.0 35.0 86-87 25.370059375229943 32.0 20.0 35.0 2.0 35.0 88-89 24.824840241576247 32.0 18.0 34.0 2.0 35.0 90-91 24.159970680296368 31.0 8.5 34.0 2.0 35.0 92-93 23.79306119214594 31.0 2.0 34.0 2.0 35.0 94-95 23.198374963916386 31.0 2.0 34.0 2.0 35.0 96-97 22.29083108341399 30.5 2.0 34.0 2.0 35.0 98-99 21.479575260509527 30.0 2.0 34.0 2.0 35.0 100-101 18.736844339542543 26.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19840269922280385 1101 2 0.29029957684248586 1101 3 0.31377469297796523 1101 4 0.2877799376700878 1101 5 0.3092015171373319 1101 6 0.34906717477075944 1101 7 0.2176668221497735 1101 8 0.24014647200821315 1101 9 0.32173969179266493 1101 10-11 0.42085442915178106 1101 12-13 0.43501508682150813 1101 14-15 0.4814868890030226 1101 16-17 0.505924477589879 1101 18-19 0.5445812418091052 1101 20-21 0.31147085482705705 1101 22-23 0.3507520011348859 1101 24-25 0.16403178154650533 1101 26-27 0.10224197789108302 1101 28-29 0.3169093465777735 1101 30-31 0.6707643147824314 1101 32-33 0.740938892060214 1101 34-35 0.6247489843404352 1101 36-37 0.9694033195120824 1101 38-39 1.3426747496753322 1101 40-41 1.3254943624839086 1101 42-43 1.4865283315896107 1101 44-45 1.5871236156081352 1101 46-47 1.2481467526772505 1101 48-49 1.4239732898500819 1101 50-51 1.845467125245797 1101 52-53 1.974648453887788 1101 54-55 1.9661169089845352 1101 56-57 1.673001336198272 1101 58-59 1.9153386199400337 1101 60-61 1.9532226768591237 1101 62-63 1.5676614991546032 1101 64-65 1.3872383589474886 1101 66-67 1.6371719112969672 1101 68-69 1.479925099103916 1101 70-71 1.5320545791135807 1101 72-73 1.5205792805085956 1101 74-75 1.400940080618021 1101 76-77 1.8194414891190718 1101 78-79 1.9141261562172787 1101 80-81 1.7220061332140943 1101 82-83 2.134800312652473 1101 84-85 1.97964763571796 1101 86-87 1.427570053304617 1101 88-89 1.6540197493358555 1101 90-91 2.002624824753749 1101 92-93 1.5492736466263572 1101 94-95 2.1812467687078225 1101 96-97 2.255401763698625 1101 98-99 2.2641760483949867 1101 100-101 2.7290655706339493 1102 1 -0.06946018814896249 1102 2 -0.013855026343463805 1102 3 -0.0018178771093673163 1102 4 0.0509544500671808 1102 5 -0.10721196536615452 1102 6 0.004986918369667137 1102 7 -0.013177561927619763 1102 8 0.23385559021244973 1102 9 0.35501702674638125 1102 10-11 0.16419121770428546 1102 12-13 -0.08596643371321733 1102 14-15 0.07909087322816077 1102 16-17 0.1982312533938142 1102 18-19 0.6994822914808552 1102 20-21 0.6323177409597136 1102 22-23 0.48794207393277844 1102 24-25 0.28328450104228153 1102 26-27 0.35279302577565375 1102 28-29 0.1708071912358946 1102 30-31 -0.005636538299263805 1102 32-33 0.12365803040003698 1102 34-35 0.3158285616122498 1102 36-37 0.49596680618564903 1102 38-39 0.4375205158403972 1102 40-41 -0.0793441358063589 1102 42-43 -0.48372103991793125 1102 44-45 -0.49822029784458266 1102 46-47 -0.37372121029232375 1102 48-49 0.08116193290211271 1102 50-51 0.1558232511110944 1102 52-53 -0.0662839787056484 1102 54-55 0.4324702230162991 1102 56-57 0.13853254635899503 1102 58-59 0.24197597681652283 1102 60-61 0.28494754609694795 1102 62-63 0.2889325432265153 1102 64-65 0.7414459818758452 1102 66-67 0.4754319105820919 1102 68-69 0.21501701984833943 1102 70-71 0.2584190928212706 1102 72-73 0.2889723653044385 1102 74-75 0.006973608158503453 1102 76-77 -0.09530991381905451 1102 78-79 -0.15830834355038803 1102 80-81 0.14308416159455106 1102 82-83 0.46897957895455633 1102 84-85 0.05100386881419183 1102 86-87 -0.1909618359853944 1102 88-89 0.4096106513863518 1102 90-91 0.52533395487254 1102 92-93 0.4306881418664936 1102 94-95 1.1068582829278384 1102 96-97 1.155962938949724 1102 98-99 0.9056077005854775 1102 100-101 1.2305548892408993 1103 1 0.02343539117124749 1103 2 0.13710199293315384 1103 3 0.11581716137575171 1103 4 0.01276904035394466 1103 5 0.0077257091791764765 1103 6 -0.2720922996672499 1103 7 0.1483100751543205 1103 8 0.04156623662618131 1103 9 0.120618824989009 1103 10-11 0.008425261706740628 1103 12-13 -0.07744521581253139 1103 14-15 0.033501335730719006 1103 16-17 -7.197717407123605E-4 1103 18-19 0.046829202454226504 1103 20-21 0.15185319494786143 1103 22-23 0.019411319896953216 1103 24-25 -0.11938561679711057 1103 26-27 0.22492707658962985 1103 28-29 0.1508902918421171 1103 30-31 0.3845821116564565 1103 32-33 0.688326649703896 1103 34-35 0.5818322398357978 1103 36-37 0.5496075663518525 1103 38-39 0.6035378171141588 1103 40-41 0.6441692488404698 1103 42-43 0.586260568964299 1103 44-45 1.1039117095118698 1103 46-47 1.3882364220215422 1103 48-49 1.2083807872035877 1103 50-51 1.0635815782005267 1103 52-53 0.5409975416267514 1103 54-55 1.0631902012194168 1103 56-57 1.7479188279474478 1103 58-59 1.6246542684860295 1103 60-61 0.8334456048122938 1103 62-63 1.0109269577759363 1103 64-65 0.989617087386371 1103 66-67 1.0649694080277712 1103 68-69 1.0033761234516376 1103 70-71 0.7167734283569978 1103 72-73 0.8043391659311041 1103 74-75 1.1040037832146972 1103 76-77 0.8814627855883437 1103 78-79 0.8269787622325957 1103 80-81 0.9875021167747171 1103 82-83 1.4196437026518538 1103 84-85 1.211156446976446 1103 86-87 1.0432933237448658 1103 88-89 1.045507388665829 1103 90-91 0.9734573797073587 1103 92-93 1.2263083312268925 1103 94-95 1.5757296132186873 1103 96-97 1.861977515613443 1103 98-99 1.822043910652912 1103 100-101 2.746725449829416 1104 1 -0.25163817642438957 1104 2 0.09670040041108052 1104 3 0.1809893960996547 1104 4 -0.07902600897962486 1104 5 -0.4336452446030634 1104 6 7.309650029938553E-4 1104 7 0.06130990205409148 1104 8 -0.11379121533850878 1104 9 -0.191930941325694 1104 10-11 0.034450880540042306 1104 12-13 0.22470988198909936 1104 14-15 0.2980157895995035 1104 16-17 0.03898076486998292 1104 18-19 0.09541843562022478 1104 20-21 0.13898319321685904 1104 22-23 -0.03269184783717094 1104 24-25 0.2107511323806719 1104 26-27 0.17497035164588937 1104 28-29 0.24290171645697 1104 30-31 -0.01746047099889836 1104 32-33 -0.2463366703584171 1104 34-35 -0.15092697775117614 1104 36-37 0.007500936613240583 1104 38-39 -0.12280803713544941 1104 40-41 0.10170776364054035 1104 42-43 -0.11324609539436636 1104 44-45 0.12480490667302035 1104 46-47 -0.07681464254483927 1104 48-49 0.10622568940195976 1104 50-51 0.6125516318615993 1104 52-53 0.4866007959109808 1104 54-55 0.02394838020505219 1104 56-57 0.12773534170890954 1104 58-59 -0.2627353801205139 1104 60-61 0.20400991154589576 1104 62-63 0.04672408431219566 1104 64-65 0.13791205016982033 1104 66-67 -0.4403101488356569 1104 68-69 -0.2844116556724785 1104 70-71 0.045066660138196823 1104 72-73 0.252374478377007 1104 74-75 0.2545144288785366 1104 76-77 0.5453021485795126 1104 78-79 0.9303628716319388 1104 80-81 0.6962090580937392 1104 82-83 0.514519935990954 1104 84-85 -0.3350872781403993 1104 86-87 -0.01814860112963501 1104 88-89 0.07637115878872791 1104 90-91 0.475223002002668 1104 92-93 -0.062422844123634746 1104 94-95 -0.07421845671380112 1104 96-97 -0.5085867911201554 1104 98-99 -0.7344992778532351 1104 100-101 0.06076387807937067 1105 1 0.1903202583256558 1105 2 0.24264685238637185 1105 3 0.07557842437151407 1105 4 0.04047408463580382 1105 5 0.2848531639346632 1105 6 0.24796930988222954 1105 7 0.46825616679852544 1105 8 0.29146997170511924 1105 9 0.46838547882035186 1105 10-11 0.0666077799970779 1105 12-13 0.38657095669420727 1105 14-15 0.25932921405185994 1105 16-17 0.1453044870193949 1105 18-19 0.45237406190744167 1105 20-21 0.7162159211704235 1105 22-23 0.6705472459654871 1105 24-25 0.5140183108116716 1105 26-27 0.499628655334341 1105 28-29 0.2543462025082661 1105 30-31 0.7954022887839187 1105 32-33 0.8528722792764825 1105 34-35 0.5954922321346103 1105 36-37 0.6404816518351026 1105 38-39 0.9303187181538206 1105 40-41 0.8699393682089678 1105 42-43 0.7659197911437374 1105 44-45 0.8228951519454384 1105 46-47 0.7299865182864664 1105 48-49 0.2785482881662418 1105 50-51 0.5219084938724521 1105 52-53 0.7814191819810077 1105 54-55 0.953900636336865 1105 56-57 0.43210250141683204 1105 58-59 0.43657186571359574 1105 60-61 -0.22652744100980726 1105 62-63 -0.7571515943996516 1105 64-65 -0.31893123760400144 1105 66-67 -0.29607217841812883 1105 68-69 0.13365144112587757 1105 70-71 0.14748193817601774 1105 72-73 0.3220711643908629 1105 74-75 0.4977273103614053 1105 76-77 0.2399052191655464 1105 78-79 0.44721364863999113 1105 80-81 0.8143985357196506 1105 82-83 0.5780380037685262 1105 84-85 0.7779065432413645 1105 86-87 0.16566529139985064 1105 88-89 0.21808151265504705 1105 90-91 -0.4047386156719277 1105 92-93 -0.2515026044681399 1105 94-95 0.2806776301613141 1105 96-97 0.960215867557995 1105 98-99 0.6302264289432458 1105 100-101 0.10644820685675427 1106 1 0.05314640144112914 1106 2 0.13879771779582484 1106 3 -0.01625831032236391 1106 4 -0.005573448301419148 1106 5 -0.1823387761325037 1106 6 0.2387985529718719 1106 7 0.12105906010774703 1106 8 0.14163788697248947 1106 9 0.5903823788461793 1106 10-11 0.38777802024507224 1106 12-13 0.5565528735115635 1106 14-15 0.41339147186637604 1106 16-17 0.36388495718214386 1106 18-19 0.39282872478524666 1106 20-21 0.5300237227720785 1106 22-23 0.5398316685952977 1106 24-25 0.7589162699953462 1106 26-27 1.0636302053214237 1106 28-29 0.834267411498189 1106 30-31 0.7726045620983015 1106 32-33 0.7567407887055708 1106 34-35 0.8835637898716229 1106 36-37 1.3541215381693874 1106 38-39 1.3604401338086873 1106 40-41 1.1714441473358121 1106 42-43 1.6016534516965635 1106 44-45 1.002693653624597 1106 46-47 1.7105793883458844 1106 48-49 2.0528637105377214 1106 50-51 1.9776369878016737 1106 52-53 1.7481267510845981 1106 54-55 1.6454209153345438 1106 56-57 1.3700707266816217 1106 58-59 1.235783955612849 1106 60-61 0.8711217452469739 1106 62-63 0.5885601080028238 1106 64-65 1.4519290052273064 1106 66-67 1.415339601483705 1106 68-69 1.187804114853595 1106 70-71 1.2983473476309406 1106 72-73 1.4844323113813047 1106 74-75 1.5008272845282882 1106 76-77 1.4165714552802449 1106 78-79 1.5241963404508923 1106 80-81 1.0097614910283603 1106 82-83 1.3206109823270396 1106 84-85 1.0164108057058456 1106 86-87 1.195502542756092 1106 88-89 1.412249686253599 1106 90-91 0.6983678167744678 1106 92-93 0.3401726732378805 1106 94-95 1.2858442537727832 1106 96-97 1.5616431473306598 1106 98-99 1.4134219856469379 1106 100-101 1.163539397763497 1107 1 0.25409576852973714 1107 2 -0.033763711839320365 1107 3 -0.023611251498834918 1107 4 0.25941165966581536 1107 5 -0.062085611575540156 1107 6 -0.04284701664838053 1107 7 0.29073515839516517 1107 8 0.34053959955625857 1107 9 0.10974202143739831 1107 10-11 0.06113058912072944 1107 12-13 -0.2667903878436064 1107 14-15 -0.29281664429147725 1107 16-17 0.0034977643303193418 1107 18-19 0.2347870270935104 1107 20-21 0.24502744578027347 1107 22-23 0.07240799026320843 1107 24-25 0.21112215234828824 1107 26-27 0.21203861931993373 1107 28-29 0.6743642097111433 1107 30-31 0.6858584712569069 1107 32-33 0.6060985697926924 1107 34-35 0.2553061577048368 1107 36-37 0.28198825447616116 1107 38-39 0.08503060290771458 1107 40-41 0.1207646983410271 1107 42-43 0.19389097961912682 1107 44-45 0.36768806911231167 1107 46-47 0.07790068395273408 1107 48-49 -0.4794799231182765 1107 50-51 -0.2042319623100184 1107 52-53 -0.1229175527128703 1107 54-55 -0.1785273348516121 1107 56-57 -0.18516940734667386 1107 58-59 -0.6610421799046513 1107 60-61 -0.2407081132787141 1107 62-63 -0.11099313101429686 1107 64-65 -0.229195343246257 1107 66-67 0.14030237140179835 1107 68-69 -0.13330534158721008 1107 70-71 -1.413817581631907 1107 72-73 -1.55405428578921 1107 74-75 -0.7448447184501177 1107 76-77 -0.6105599669088839 1107 78-79 -0.7782515374344392 1107 80-81 -0.7397731329478141 1107 82-83 -0.6596570742626859 1107 84-85 0.06699717949586415 1107 86-87 -0.6410573976757767 1107 88-89 -0.3144815125550373 1107 90-91 -0.3168034416022998 1107 92-93 -0.42969888297954384 1107 94-95 -0.08194241785789558 1107 96-97 0.37363123370445095 1107 98-99 0.4887198263021908 1107 100-101 0.8526216762445102 1108 1 0.2794225659153611 1108 2 0.3044715822783246 1108 3 0.3170750230109718 1108 4 0.1932351890775834 1108 5 0.09804510737870942 1108 6 0.3051921990378972 1108 7 0.26050640022523197 1108 8 -0.1423035376986448 1108 9 0.43000345934589035 1108 10-11 0.3556567329115765 1108 12-13 0.190725952025673 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6 36.0 7 70.0 8 130.0 9 265.0 10 466.0 11 721.0 12 957.0 13 1213.0 14 1575.0 15 2140.0 16 2890.0 17 3803.0 18 3433.0 19 2143.0 20 1953.0 21 2054.0 22 2183.0 23 2242.0 24 2533.0 25 2587.0 26 2934.0 27 3406.0 28 3921.0 29 4608.0 30 5578.0 31 6581.0 32 8111.0 33 10589.0 34 14974.0 35 21664.0 36 26830.0 37 24652.0 38 9089.0 39 320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.28886196628132 19.52467758919372 15.639896285057574 12.546564159467385 2 13.911640675855432 29.64197775450712 35.30240851328786 21.143973056349587 3 27.02563493006854 32.42147922998987 29.78553599021922 10.767349849722368 4 10.744709151936064 18.777062709072695 40.30157409451359 30.17665404447765 5 10.327671208064707 45.40020716472992 30.418121819653482 13.85399980755189 6 22.846728136161158 41.55020857742836 16.383940953060172 19.219122333350313 7 22.6723947632066 42.11566000464134 19.226480560130863 15.985464672021191 8 24.05857148517317 28.791043339955735 19.308553089606225 27.841832085264866 9 25.060422362217206 25.433427858246592 22.316935807961602 27.189213971574606 10-11 23.72264013176886 28.53322239391418 23.923010307177666 23.82112716713929 12-13 34.22687532018352 28.669480036115285 22.169860718399832 14.933783925301361 14-15 26.333589938075235 28.89237317876671 31.666251570760647 13.107785312397406 16-17 15.704154002026344 32.47270380873138 31.647733383142867 20.175408806099405 18-19 15.759102293792186 36.524077035192235 33.10164287028258 14.615177800732996 20-21 20.7479460351332 28.71050911159475 30.931309981009846 19.610234872262204 22-23 17.58597853649701 30.85633263901467 30.653697246875566 20.90399157761275 24-25 21.885250309189654 30.95292675955092 31.45696957347393 15.704853357785495 26-27 22.017008872348555 26.935997396312384 36.714259434829984 14.332734296509079 28-29 16.210785492931837 32.94428957534421 35.585051436980855 15.259873494743099 30-31 17.40362137961092 34.52423403689301 32.09403813825542 15.978106445240641 32-33 16.853878525964856 34.41585088709837 31.392726048060947 17.337544538875825 34-35 19.086391242578095 30.712955573290767 32.09460415570008 18.106049028431055 36-37 20.239938190020403 30.941781451911893 34.68264367848622 14.13563667958148 38-39 20.800772128646596 30.52568714637895 34.4585051612045 14.215035563769957 40-41 15.500519325414682 33.52181874269478 35.128472900269706 15.849189031620833 42-43 14.982764768810174 36.84518856871169 29.62931517549371 18.54273148698443 44-45 18.07257557833487 33.262108665259156 29.390788527828565 19.274527228577412 46-47 19.227721427277665 33.83926145625792 27.85076299583409 19.08225412063032 48-49 20.357441027890587 33.02098515615051 29.19441339201064 17.427160423948266 50-51 19.1276858287862 34.24700568297598 32.529377136776326 14.095931351461498 52-53 17.68441608060111 36.72331573957464 29.443461772488643 16.148806407335606 54-55 13.588663802618397 34.10849422379198 31.166335546461543 21.136506427128086 56-57 19.14219813496724 34.644327781629855 27.47739461432877 18.736079469074134 58-59 21.048043832639017 32.424492845499 27.292666636478895 19.23479668538308 60-61 17.45609020258223 32.554664372357344 31.713826421160473 18.275419003899948 62-63 19.966265360298404 31.92677998335909 30.096279567336264 18.01067508900624 64-65 18.20821517719176 35.754189944134076 29.978547938847477 16.059046939826686 66-67 16.866753833353144 31.830557017767287 29.909210801876913 21.393478347002652 68-69 15.70589650341734 36.23257722622366 28.548019640860918 19.51350662949808 70-71 19.840324219380147 34.34614154598532 25.900475744394225 19.913058490240306 72-73 17.96140272428051 31.56469246422012 29.520774549231476 20.953130262267894 74-75 17.14544476834687 36.30855008066114 27.68233662581722 18.863668525174766 76-77 19.071674789017 31.410006056386656 28.49162011173184 21.026699042864504 78-79 16.79138058877801 31.98063066377617 29.927379139756948 21.300609607688866 80-81 17.91346382439179 34.652677934633715 27.644821301212456 19.789036939762035 82-83 17.085850938884093 31.93111548203597 28.774993278523826 22.208040300556114 84-85 16.52991970656244 31.537462251895544 28.526104565433354 23.40651347610867 86-87 17.22523787152391 32.975417584096995 28.178648448245607 21.62069609613349 88-89 17.974437060601602 31.27639220658659 26.92005071832086 23.829120014490947 90-91 16.057282748666278 29.912123056023322 29.411465040259245 24.619129155051155 92-93 16.880904269469585 33.84076797246891 27.409677766268757 21.868649991792747 94-95 17.220797744986502 33.508515732454875 26.21679600165277 23.053890520905853 96-97 13.837828965056618 32.67306837831796 28.243378247198898 25.245724409426533 98-99 17.46344859596194 33.91511810221374 24.903916363368786 23.717516938455528 100-101 16.759969433673902 32.51860866611949 25.39948490080095 25.321936999405654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3080.0 1 2049.5 2 1002.5 3 900.0 4 775.5 5 861.5 6 1277.5 7 2126.5 8 2756.0 9 2704.5 10 2233.0 11 1839.0 12 1778.5 13 1680.5 14 1514.0 15 1300.0 16 1100.5 17 1009.0 18 939.0 19 886.5 20 873.0 21 857.5 22 880.5 23 956.0 24 1032.0 25 1119.0 26 1255.0 27 1391.5 28 1522.5 29 1687.5 30 1766.0 31 1832.0 32 2055.0 33 2231.5 34 2383.5 35 2559.5 36 2735.5 37 2895.0 38 2825.0 39 2630.0 40 2416.5 41 2228.0 42 2088.5 43 2041.0 44 2170.0 45 2469.0 46 4029.0 47 9935.5 48 28792.0 49 27765.0 50 9320.5 51 4351.5 52 1887.0 53 1188.5 54 1025.5 55 966.0 56 1046.5 57 1080.5 58 1009.0 59 993.5 60 1035.0 61 1049.0 62 844.5 63 444.0 64 207.0 65 137.0 66 97.0 67 81.5 68 51.5 69 35.5 70 33.0 71 20.0 72 15.0 73 17.5 74 12.5 75 9.0 76 6.5 77 4.0 78 3.0 79 2.0 80 2.0 81 2.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019810610563017552 2 0.004528139557261154 3 0.0 4 0.0 5 0.0011320348893152886 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.009339287836851132 14-15 0.00396212211260351 16-17 0.0 18-19 2.8300872232882215E-4 20-21 0.001415043611644111 22-23 5.660174446576443E-4 24-25 8.490261669864666E-4 26-27 2.8300872232882215E-4 28-29 2.8300872232882215E-4 30-31 0.0 32-33 8.490261669864666E-4 34-35 0.0 36-37 0.001415043611644111 38-39 0.01047132272616642 40-41 8.490261669864666E-4 42-43 0.0 44-45 0.0011320348893152886 46-47 5.660174446576443E-4 48-49 2.8300872232882215E-4 50-51 0.002830087223288222 52-53 2.8300872232882215E-4 54-55 0.0 56-57 2.8300872232882215E-4 58-59 5.660174446576443E-4 60-61 0.002264069778630577 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.8300872232882215E-4 70-71 0.001415043611644111 72-73 0.001415043611644111 74-75 0.004528139557261154 76-77 0.0 78-79 0.0011320348893152886 80-81 0.00396212211260351 82-83 2.8300872232882215E-4 84-85 0.004811148279589977 86-87 0.0011320348893152886 88-89 0.006226191891234088 90-91 0.003113095945617044 92-93 0.0 94-95 0.0 96-97 8.490261669864666E-4 98-99 0.002264069778630577 100-101 0.004528139557261154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 176673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.90655618006147 #Duplication Level Percentage of deduplicated Percentage of total 1 82.65648382269467 27.19940228557844 2 9.389889399177804 6.179778460772161 3 2.996370641759981 2.9580071657808493 4 1.4672239709651342 1.9312515211718826 5 0.7275917229991227 1.1971268954509178 6 0.5125823485903985 1.012039191047868 7 0.3577755990161171 0.8241213994215301 8 0.2803722242289764 0.7380867478335682 9 0.17888779950805855 0.5297923281995551 >10 1.1472900218449524 7.08597238966905 >50 0.11868517467361578 2.8278231535095912 >100 0.09460412473983866 6.660893288731158 >500 0.025801124929046907 5.595648457885472 >1k 0.04300187488174484 28.159933889162463 >5k 0.003440149990539588 7.1001228257854905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6778 3.8364662398895133 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5766 3.263656585895977 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4772 2.701035245906279 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3841 2.174073004930012 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3523 1.9940794575288812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2889 1.6352243976159346 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2868 1.623338031278124 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2802 1.5859808799307196 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2674 1.5135306470145409 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 1.2599548318079163 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2169 1.2276918374624306 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2022 1.1444872730977569 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1773 1.0035489293780036 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1575 0.8914774753357899 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1502 0.8501582018757818 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1498 0.8478941320971511 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1446 0.8184612249749538 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1416 0.8014807016352244 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1403 0.7941224748546751 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1324 0.7494070967267211 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1261 0.7137479977132895 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1255 0.7103518930453436 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1210 0.6848811080357496 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1105 0.625449276346697 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1098 0.6214871542340935 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1066 0.6033745960050488 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1033 0.5846960203313466 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 968 0.5479048864285997 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 853 0.48281288029297065 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 828 0.46866244417652947 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.4052684903748734 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 675 0.38206177514390993 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 666 0.37696761814199115 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 653 0.3696093913614418 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 632 0.35772302502363124 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 631 0.35715700757897356 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 609 0.3447046237965054 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 549 0.31074357711704675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 546 0.30904552478307384 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 527 0.29829119333457854 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 517 0.29263101888800214 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 516 0.29206500144334446 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 492 0.278480582771561 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 460 0.2603680245425164 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 458 0.2592359896532011 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 388 0.219614768527166 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.21338857663593191 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 367 0.20772840218935545 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.20320026263209431 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 358 0.2026342451874367 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 322 0.18225761717976147 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 312 0.17659744273318503 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 306 0.17320133806523916 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 292 0.16527709384003214 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 270 0.15282471005756398 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 268 0.1516926751682487 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 266 0.1505606402789334 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 257 0.1454664832770146 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 254 0.14376843094304168 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 249 0.14093834371975344 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 247 0.13980630883043815 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 234 0.13244808204988878 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 230 0.1301840122712582 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 230 0.1301840122712582 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.12508985526933938 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 219 0.1239578203800241 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 205 0.11603357615481709 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 185 0.10471322726166421 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 185 0.10471322726166421 No Hit AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA 181 0.10244915748303363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.8300872232882215E-4 0.0 16-17 0.0 0.0 0.0 0.0011320348893152886 0.0 18-19 0.0 0.0 0.0 0.003113095945617044 0.0 20-21 0.0 0.0 0.0 0.005660174446576443 0.0 22-23 0.0 0.0 0.0 0.01047132272616642 0.0 24-25 0.0 0.0 0.0 0.017546540784386975 0.0 26-27 0.0 0.0 0.0 0.028300872232882218 0.0 28-29 0.0 0.0 0.0 0.04584741301726919 0.0 30-31 0.0 0.0 0.0 0.07329925908316495 0.0 32-33 0.0 0.0 0.0 0.09084579986755192 0.0 34-35 0.0 0.0 0.0 0.1134864976538577 0.0 36-37 0.0 0.0 0.0 0.13952330010810932 0.0 38-39 0.0 0.0 0.0 0.1661261200070186 0.0 40-41 0.0 0.0 0.0 0.22385989936209832 0.0 42-43 0.0 0.0 0.0 0.33706338829362725 0.0 44-45 0.0 0.0 0.0 0.4429086504446067 0.0 46-47 0.0 0.0 0.0 0.6928053522609567 0.0 48-49 0.0 0.0 0.0 0.9101560510094922 0.0 50-51 0.0 0.0 0.0 1.0502453685622593 0.0 52-53 0.0 0.0 0.0 1.1931647733383142 0.0 54-55 0.0 0.0 0.0 1.3476875357298512 0.0 56-57 0.0 0.0 0.0 1.6796567670215596 0.0 58-59 0.0 0.0 0.0 2.1364328448602787 0.0 60-61 0.0 0.0 0.0 2.6382073095492804 0.0 62-63 0.0 0.0 0.0 3.085644099551148 0.0 64-65 0.0 0.0 0.0 3.5277037238287683 0.0 66-67 0.0 0.0 0.0 4.10390948249025 0.0 68-69 0.0 0.0 0.0 4.69737877321379 0.0 70-71 0.0 0.0 0.0 5.114250621204145 0.0 72-73 0.0 0.0 0.0 5.534518573862446 0.0 74-75 0.0 0.0 0.0 6.028368794326241 0.0 76-77 0.0 0.0 0.0 6.47806965410674 0.0 78-79 0.0 0.0 0.0 6.949562185506558 0.0 80-81 0.0 0.0 0.0 7.599067203251204 0.0 82-83 0.0 0.0 0.0 8.299796799737369 0.0 84-85 0.0 0.0 0.0 9.610127184119815 0.0 86-87 0.0 0.0 0.0 10.871214050817047 0.0 88-89 0.0 0.0 0.0 11.885234302921216 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 20 1.739909E-5 94.998024 3 GACATAT 20 1.739909E-5 94.998024 1 TAAGGTG 25 0.005014676 56.998814 5 AGGATAT 50 0.0016100389 38.009968 8 TATAGGA 75 6.8900845E-6 37.99921 5 ACATATA 50 0.0016122808 37.99921 2 GATATGA 65 1.3238011E-4 36.537697 2 TGGGGGA 65 1.3238011E-4 36.537697 6 ATGGGGG 120 3.756213E-9 35.624256 5 TGGGACT 95 8.6460386E-7 34.99927 6 AGTACTC 55 0.0025740073 34.544735 3 GATTGGC 70 2.0431781E-4 33.937466 9 ATAGGAT 70 2.0465809E-4 33.927864 6 GGATATG 70 2.0465809E-4 33.927864 1 CATGGGT 85 1.6246597E-5 33.528713 4 ATATGAG 85 1.6246597E-5 33.528713 3 AAGTACT 60 0.003942387 31.666006 2 GCTTGTC 30 0.003947684 31.666006 94-95 GGGACCT 60 0.003942387 31.666006 5 CAGTGGA 120 0.0 31.666006 94-95 >>END_MODULE