FastQCFastQC Report
Thu 26 May 2016
SRR937023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918009
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.48267500645418504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.3730900241718763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21020.2289737900173092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.049.472211
GTATCAA35700.040.3366741
ATCAACG44700.032.0025523
TATCAAC45500.031.4398752
CAACGCA45800.031.1267725
TCAACGC46150.030.9987474
AACGCAG46900.030.396726
ACGCAGA53850.026.2089167
CGCAGAG53500.026.204168
TATAGGG2800.025.460032
GCAGAGT56850.024.0748739
GTACATG30050.023.0905381
TACATGG29650.023.081472
ACATGGG30150.022.6986943
GAGTACT36900.022.277312-13
GTACTTT38450.021.25509514-15
CAGAGTA54550.021.25386210-11
AGAGTAC50350.021.13933610-11
GTGTAGG3900.020.7161941
AGTACTT37800.020.55269412-13
Produced by FastQC (version 0.11.5)