FastQCFastQC Report
Thu 26 May 2016
SRR937023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918009
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41060.44727230343057633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.3917172925319904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.24705640140782933No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.10991177646406518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.042.4305951
GTATCAA41950.034.880551
CTAGCGT650.00584657929.2275854
TATCAAC51550.028.284992
ATCAACG51950.027.8843543
TCAACGC52450.027.5279854
CAACGCA52600.027.449485
AACGCAG54700.026.3956626
TACATGG34050.023.433572
GTACATG35950.023.390471
ACGCAGA61300.023.3212767
GTCTAGA4300.023.2014681
CGCAGAG61450.023.187068
GAGTACT36050.022.72637412-13
ACATGGG34850.021.805373
GTACTTT38550.021.12992114-15
GCAGAGT66750.020.5633039
AGAGTAC56000.020.3125210-11
AGTACTT38550.019.89731412-13
CAGAGTA62000.019.22774310-11
Produced by FastQC (version 0.11.5)