##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937023_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 918009 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.148684816815525 33.0 31.0 34.0 31.0 34.0 2 32.144965899027135 33.0 31.0 34.0 30.0 34.0 3 32.33324400959032 34.0 31.0 34.0 31.0 34.0 4 36.063500466771025 37.0 35.0 37.0 35.0 37.0 5 35.992459768912944 37.0 35.0 37.0 35.0 37.0 6 36.03230469418056 37.0 35.0 37.0 35.0 37.0 7 35.973873894482516 37.0 35.0 37.0 35.0 37.0 8 35.959642007867025 37.0 35.0 37.0 35.0 37.0 9 37.65454695977926 39.0 37.0 39.0 35.0 39.0 10-11 37.57712506086541 39.0 37.0 39.0 35.0 39.0 12-13 37.48363795997643 39.0 37.0 39.0 35.0 39.0 14-15 38.72090360769883 40.0 38.0 41.0 34.5 41.0 16-17 38.62973783481425 40.0 38.0 41.0 34.0 41.0 18-19 38.683649615635574 40.0 38.0 41.0 34.5 41.0 20-21 38.75270340486858 40.0 38.0 41.0 34.0 41.0 22-23 38.67949224898666 40.0 38.0 41.0 34.0 41.0 24-25 38.572372928805706 40.0 38.0 41.0 34.0 41.0 26-27 38.41453188367434 40.0 38.0 41.0 34.0 41.0 28-29 38.24730367567203 40.0 38.0 41.0 34.0 41.0 30-31 38.11125435589412 40.0 38.0 41.0 33.5 41.0 32-33 38.1069787986828 40.0 37.0 41.0 34.0 41.0 34-35 38.02382874241974 40.0 37.0 41.0 33.0 41.0 36-37 37.8633145208816 40.0 37.0 41.0 33.0 41.0 38-39 37.71516346789629 40.0 37.0 41.0 33.0 41.0 40-41 37.54079535167956 40.0 37.0 41.0 33.0 41.0 42-43 37.3221831158518 40.0 37.0 41.0 32.0 41.0 44-45 36.971059107263656 40.0 36.0 41.0 31.0 41.0 46-47 36.72795201354235 39.0 36.0 41.0 31.0 41.0 48-49 36.39967364154382 39.0 36.0 41.0 30.0 41.0 50-51 36.13259619459068 39.0 35.0 40.5 30.0 41.0 52-53 35.87933233770039 39.0 35.0 40.0 29.0 41.0 54-55 35.63346601177113 39.0 35.0 40.0 28.0 41.0 56-57 35.34983916279688 38.0 35.0 40.0 28.0 41.0 58-59 35.031961560289716 38.0 34.0 40.0 27.0 41.0 60-61 34.69280747792233 38.0 34.0 40.0 26.5 41.0 62-63 34.30797083688722 37.0 34.0 40.0 26.0 41.0 64-65 33.903382210849784 36.5 33.0 39.0 26.0 41.0 66-67 33.52393440587184 36.0 33.0 39.0 25.0 40.0 68-69 33.0795901783098 35.5 32.5 39.0 23.5 40.0 70-71 32.6050120423656 35.0 32.0 38.0 23.0 40.0 72-73 32.26929256684848 35.0 31.5 37.5 22.0 39.5 74-75 31.827992971746465 35.0 31.0 37.0 21.5 39.0 76-77 30.425495828472272 33.5 29.5 35.5 20.0 38.0 78-79 31.158525678942148 34.0 31.0 36.0 20.0 38.0 80-81 31.005247769902038 34.0 31.0 36.0 20.0 37.5 82-83 30.682740583153326 34.0 31.0 35.0 18.5 37.0 84-85 30.309116250494277 34.0 30.5 35.0 17.5 36.5 86-87 29.955559259222948 34.0 30.0 35.0 12.0 36.0 88-89 29.60236337552246 34.0 30.0 35.0 7.0 36.0 90-91 29.198443588243688 34.0 29.0 35.0 3.5 35.0 92-93 28.75640271500606 34.0 29.0 35.0 2.0 35.0 94-95 28.28525101605758 34.0 29.0 35.0 2.0 35.0 96-97 27.666593137975774 33.0 27.5 35.0 2.0 35.0 98-99 26.97207652648286 33.0 26.5 35.0 2.0 35.0 100-101 24.00180063594148 29.5 19.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2129672506988598 1101 2 0.14939534934990206 1101 3 0.14472740762862202 1101 4 0.17502421962299763 1101 5 0.2515657562668068 1101 6 0.16222601457694452 1101 7 0.2267980287842022 1101 8 0.22268585540280128 1101 9 0.31723762465050953 1101 10-11 0.3250274489526106 1101 12-13 0.18806143118792562 1101 14-15 0.29653577177060697 1101 16-17 0.2809695665841687 1101 18-19 0.2598863151065629 1101 20-21 0.2868688676584057 1101 22-23 0.25698540158298044 1101 24-25 0.2776556819697973 1101 26-27 0.23571599008008803 1101 28-29 0.2799431578202771 1101 30-31 0.2724817132561981 1101 32-33 0.36886087535688716 1101 34-35 0.2732166413327377 1101 36-37 0.23090544006044667 1101 38-39 0.22179372175340006 1101 40-41 0.19622703118690055 1101 42-43 0.10802358167143211 1101 44-45 0.29349887075375847 1101 46-47 0.46604276037103176 1101 48-49 0.37559936666342963 1101 50-51 0.27495571302587507 1101 52-53 0.030563443055008577 1101 54-55 0.25731946008473017 1101 56-57 0.3953401956017615 1101 58-59 0.2573409602461467 1101 60-61 0.2955178807751011 1101 62-63 0.40831126806642004 1101 64-65 0.29590953217557114 1101 66-67 0.4217076470102157 1101 68-69 0.4769596916869645 1101 70-71 0.42050037396598583 1101 72-73 0.5493279358470176 1101 74-75 0.31067538936407857 1101 76-77 0.12570346644717745 1101 78-79 0.331674708278058 1101 80-81 0.38470886970295126 1101 82-83 0.4365735393700838 1101 84-85 0.39423972788521056 1101 86-87 0.36654091666520117 1101 88-89 0.16856500138411334 1101 90-91 0.3674374538714176 1101 92-93 0.5502105738130005 1101 94-95 0.5271635376601722 1101 96-97 0.6717399887726678 1101 98-99 0.4357166456239767 1101 100-101 0.30046241640121707 1102 1 0.2805008971082117 1102 2 0.13666441461935364 1102 3 0.1718421373304011 1102 4 0.19231235556509318 1102 5 0.07937960674161815 1102 6 0.07326590655439702 1102 7 0.18446998645616475 1102 8 0.19309440828558166 1102 9 0.31636642527707437 1102 10-11 0.28568479538120783 1102 12-13 0.3533168372038844 1102 14-15 0.44537424121790536 1102 16-17 0.36512032472831635 1102 18-19 0.4128505027916205 1102 20-21 0.3279593231623963 1102 22-23 0.27979482144550616 1102 24-25 0.24500834738703503 1102 26-27 0.4592766585931045 1102 28-29 0.39506574838539166 1102 30-31 0.44155254027295143 1102 32-33 0.5167190525381571 1102 34-35 0.4396584325556958 1102 36-37 0.4024169632545238 1102 38-39 0.3898050097802468 1102 40-41 0.44967838038810015 1102 42-43 0.35501503640456633 1102 44-45 0.5033606624089799 1102 46-47 0.5247903122133124 1102 48-49 0.3205272675823849 1102 50-51 0.42881065447078015 1102 52-53 0.575036550113218 1102 54-55 0.5156087188602712 1102 56-57 0.39844960033536125 1102 58-59 0.4164079175527391 1102 60-61 0.3085513939058657 1102 62-63 0.3505869766813632 1102 64-65 0.3671227480590886 1102 66-67 0.4596166553043659 1102 68-69 0.2650971775486326 1102 70-71 0.17174799527779072 1102 72-73 0.5009804873342318 1102 74-75 0.41489435114965545 1102 76-77 0.49462809869606517 1102 78-79 0.586130792728305 1102 80-81 0.49689084076451806 1102 82-83 0.4619175679236065 1102 84-85 0.45636452809598893 1102 86-87 0.4436665041973207 1102 88-89 0.4675433361531489 1102 90-91 0.3471227620434014 1102 92-93 0.28517633819911126 1102 94-95 0.4718199928204214 1102 96-97 0.5728822736924322 1102 98-99 0.7730604292330767 1102 100-101 0.7713171180475946 1103 1 -0.0258397910288366 1103 2 -0.16628580091382972 1103 3 0.07334806757521051 1103 4 0.0908604487184661 1103 5 0.07266030649032729 1103 6 0.011191677060310212 1103 7 0.12532669443615418 1103 8 0.18412674835054332 1103 9 0.11198313262941184 1103 10-11 0.1893286847968838 1103 12-13 0.2063238781787291 1103 14-15 0.13009167194578453 1103 16-17 0.32093826363480815 1103 18-19 0.26973669275223244 1103 20-21 0.22072343817619355 1103 22-23 0.18312432158336378 1103 24-25 0.2681666762931414 1103 26-27 0.28539865284106725 1103 28-29 0.2928376754963935 1103 30-31 0.16915804841792692 1103 32-33 0.22293600243537526 1103 34-35 0.18759780978471952 1103 36-37 0.3298950344622895 1103 38-39 0.38018951739395845 1103 40-41 0.2389478988625413 1103 42-43 0.30516276013653254 1103 44-45 0.4454306552133289 1103 46-47 0.2822336604983491 1103 48-49 0.267671200709934 1103 50-51 0.2142021447768272 1103 52-53 0.33071517141817 1103 54-55 0.24238594295426452 1103 56-57 0.20621820982689343 1103 58-59 0.3146220071239796 1103 60-61 0.38601164411713995 1103 62-63 0.32841216503472737 1103 64-65 0.1777696166073781 1103 66-67 0.33902286866764797 1103 68-69 0.3588221693106206 1103 70-71 0.38853929683819644 1103 72-73 0.38555703131622465 1103 74-75 0.4697101672280084 1103 76-77 0.4160431082342235 1103 78-79 0.34505025751558804 1103 80-81 0.5017742421314679 1103 82-83 0.4377079123059566 1103 84-85 0.5155649818028678 1103 86-87 0.28529444662317616 1103 88-89 0.5040091299272333 1103 90-91 0.6787558153965492 1103 92-93 0.7267683023867519 1103 94-95 0.6069334083320825 1103 96-97 0.9529256717417596 1103 98-99 0.9562406589109784 1103 100-101 1.046221680446859 1104 1 0.12918704546880377 1104 2 -0.014146891997704358 1104 3 0.0884325825155301 1104 4 0.12386934081171574 1104 5 0.05723532852029223 1104 6 0.07064853839171548 1104 7 0.18284091377445577 1104 8 0.15523946164061897 1104 9 0.11839985937778863 1104 10-11 0.12012656992065018 1104 12-13 0.10649811514099383 1104 14-15 0.2364563278778533 1104 16-17 0.23160677280727526 1104 18-19 0.3120682636887082 1104 20-21 0.10589126642260993 1104 22-23 0.18678329779100977 1104 24-25 0.16565726349173104 1104 26-27 0.1955664810874822 1104 28-29 0.3008385566948206 1104 30-31 0.22293318260041417 1104 32-33 0.2199339196027239 1104 34-35 -0.03787883845688356 1104 36-37 0.19852837580652505 1104 38-39 0.18999685537361444 1104 40-41 0.10461921184819545 1104 42-43 0.3069431815095953 1104 44-45 0.15509179436257625 1104 46-47 0.12742512088446745 1104 48-49 0.001593334812312719 1104 50-51 -0.2032275596211548 1104 52-53 0.040858966980131584 1104 54-55 -0.053474427149076575 1104 56-57 -0.06588585169678396 1104 58-59 -0.014747935998663309 1104 60-61 0.054596920092471635 1104 62-63 0.04933061412548767 1104 64-65 -0.11578361578867913 1104 66-67 -0.15548165748792542 1104 68-69 -0.17641575426544165 1104 70-71 -0.16781935092276257 1104 72-73 -0.031720466080237486 1104 74-75 -0.13801433809923935 1104 76-77 -0.2668716558449269 1104 78-79 -0.27951915688800355 1104 80-81 -0.17581928506552202 1104 82-83 -0.20373044385842576 1104 84-85 0.057203610337676736 1104 86-87 -0.12939953719481778 1104 88-89 -0.057063929433496696 1104 90-91 0.1293586352315259 1104 92-93 0.0725946261871222 1104 94-95 -0.09254388078110765 1104 96-97 0.044217829075734016 1104 98-99 0.16906009334180538 1104 100-101 0.33503246311128265 1105 1 0.05654334408482242 1105 2 -0.09785400045984005 1105 3 -0.10361375213106783 1105 4 -0.043014519510876426 1105 5 -0.0237004562391121 1105 6 -0.020145144471868548 1105 7 0.052169465480019994 1105 8 -0.0012780317088783022 1105 9 0.06359971121467822 1105 10-11 0.040409763509224206 1105 12-13 0.12536786629431163 1105 14-15 0.20695839879410727 1105 16-17 0.02762573095119336 1105 18-19 -0.09321930744641804 1105 20-21 -0.08363797895571423 1105 22-23 0.13292011295752104 1105 24-25 -0.005037587840050151 1105 26-27 -0.17155393511617234 1105 28-29 -0.059993345517519 1105 30-31 0.007668172666754458 1105 32-33 0.04071632186558105 1105 34-35 0.023596226364475115 1105 36-37 -0.16063584953610643 1105 38-39 -0.07613861285550172 1105 40-41 -0.016085741514096696 1105 42-43 0.1405703155635436 1105 44-45 0.09488200886235632 1105 46-47 0.06419567009820071 1105 48-49 -0.141937998318987 1105 50-51 0.03122288991920641 1105 52-53 0.12140498172909275 1105 54-55 0.1707157267385213 1105 56-57 -0.025365765492786352 1105 58-59 0.031005002008548388 1105 60-61 0.06207073899673787 1105 62-63 0.11931158863470159 1105 64-65 -0.048249016334182215 1105 66-67 0.07134596819817318 1105 68-69 -0.03387189461631834 1105 70-71 -0.17014365977551194 1105 72-73 -0.21771147527307733 1105 74-75 -0.34228497149655013 1105 76-77 -0.263624694872437 1105 78-79 -0.1832468061455046 1105 80-81 0.09027998422094186 1105 82-83 0.10965727207247511 1105 84-85 0.1698841841330072 1105 86-87 0.13748348129189836 1105 88-89 0.10328475898620937 1105 90-91 0.304111135484078 1105 92-93 0.15578316374983814 1105 94-95 0.16956761196226466 1105 96-97 0.3198971906274046 1105 98-99 0.12057455608214696 1105 100-101 0.13986039185829569 1106 1 0.30620694948893856 1106 2 0.1975162344282282 1106 3 0.12386645868577517 1106 4 0.1989342452252103 1106 5 0.2512680560790983 1106 6 0.18803505400811105 1106 7 0.27629146841217533 1106 8 0.1926244038166871 1106 9 0.2953174897766573 1106 10-11 0.3950026614948783 1106 12-13 0.33896408949392054 1106 14-15 0.37668919704629644 1106 16-17 0.3317070796281385 1106 18-19 0.28530207692134013 1106 20-21 0.35499833903687517 1106 22-23 0.21445814634736848 1106 24-25 0.17146915870061008 1106 26-27 0.26160180482283124 1106 28-29 0.28617605964502246 1106 30-31 0.1418085661264996 1106 32-33 0.3898910433538063 1106 34-35 0.4325896534005622 1106 36-37 0.4006525202483431 1106 38-39 0.5512961237238585 1106 40-41 0.3803075919913823 1106 42-43 0.451221759119548 1106 44-45 0.6866668081301768 1106 46-47 0.6309122580098006 1106 48-49 0.3240423946817188 1106 50-51 0.42616233889194177 1106 52-53 0.35322030755128964 1106 54-55 0.5371419680991352 1106 56-57 0.38972149626954433 1106 58-59 0.16927967787908216 1106 60-61 0.3954119621820027 1106 62-63 0.4148152337462889 1106 64-65 0.5518251482739487 1106 66-67 0.3898176895343397 1106 68-69 0.501255160690107 1106 70-71 0.4813565597058229 1106 72-73 0.5390745146451223 1106 74-75 0.8984953778947897 1106 76-77 0.6318204218830239 1106 78-79 0.8091638735580702 1106 80-81 0.7754670024020243 1106 82-83 0.8683547579352933 1106 84-85 0.7649626379515517 1106 86-87 0.5131049864461268 1106 88-89 0.7712245608505093 1106 90-91 1.0297106925047572 1106 92-93 1.1203957433297518 1106 94-95 1.0396074055063593 1106 96-97 1.215353066271863 1106 98-99 1.1445439262424415 1106 100-101 1.0178603287394807 1107 1 0.043846800918032613 1107 2 0.05885948758762538 1107 3 -0.04838294114210129 1107 4 0.04779278578779156 1107 5 0.016153457175263952 1107 6 0.011624083314629274 1107 7 0.08580476279093574 1107 8 -0.03909865638171084 1107 9 0.09676243247720606 1107 10-11 0.06600642106365484 1107 12-13 -0.0012570682720820514 1107 14-15 -0.04255084915563856 1107 16-17 0.11385026031391732 1107 18-19 0.11036039553347621 1107 20-21 0.13079158540187308 1107 22-23 0.0410961632880813 1107 24-25 0.05089118162039341 1107 26-27 0.22450720410714808 1107 28-29 0.1815536018835573 1107 30-31 0.18167188541806922 1107 32-33 0.14873508919336587 1107 34-35 0.24298905096363654 1107 36-37 0.30366527107499763 1107 38-39 0.0892060526091285 1107 40-41 0.1292204877652594 1107 42-43 0.1171438726313454 1107 44-45 0.04396739244869252 1107 46-47 0.09227759297378668 1107 48-49 0.14963294140455474 1107 50-51 0.13956882528049164 1107 52-53 0.22426539972866522 1107 54-55 0.12705454680095585 1107 56-57 0.3310971360756767 1107 58-59 0.13350432343780483 1107 60-61 0.2661399822934314 1107 62-63 0.09298425580545455 1107 64-65 0.10333520398803842 1107 66-67 0.06982403097565992 1107 68-69 0.313981640737218 1107 70-71 0.24681382292093446 1107 72-73 0.3261011713466715 1107 74-75 0.23370283361793298 1107 76-77 0.36860814213487103 1107 78-79 0.3551166751162498 1107 80-81 0.30244360701316 1107 82-83 0.2076331925052095 1107 84-85 0.33236309997084845 1107 86-87 0.5002522437727528 1107 88-89 0.379173444303877 1107 90-91 0.7924700121586952 1107 92-93 0.906977932938851 1107 94-95 0.5744683467574347 1107 96-97 0.8263084723505862 1107 98-99 0.7009912469834845 1107 100-101 0.5458844035684862 1108 1 -0.0974221604728811 1108 2 -0.2365290669472735 1108 3 -0.09558844566468139 1108 4 -0.02397893842295673 1108 5 0.005652102161711525 1108 6 0.05792771193533497 1108 7 0.004446757652445399 1108 8 -0.0021173450101557023 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64-65 -0.6648076733838124 2316 66-67 -0.8191120588950085 2316 68-69 -0.9599551571481797 2316 70-71 -0.6893232487301475 2316 72-73 -0.46125250560981357 2316 74-75 -0.544110344896179 2316 76-77 -0.8363208203875452 2316 78-79 -0.613117064145527 2316 80-81 -0.678044687143359 2316 82-83 -0.6795800615304728 2316 84-85 -0.7145074749371112 2316 86-87 -0.6696948911276515 2316 88-89 -0.6870908339931141 2316 90-91 -0.6917353593130358 2316 92-93 -0.9639627826280339 2316 94-95 -0.7269217221559998 2316 96-97 -0.7882778110441073 2316 98-99 -0.6800563788071301 2316 100-101 -0.8437065999243849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 4.0 5 17.0 6 39.0 7 109.0 8 257.0 9 465.0 10 778.0 11 1293.0 12 1923.0 13 2515.0 14 3357.0 15 4491.0 16 6084.0 17 8374.0 18 7887.0 19 5354.0 20 4561.0 21 4917.0 22 4761.0 23 5317.0 24 6267.0 25 7335.0 26 8957.0 27 11046.0 28 13689.0 29 16972.0 30 21379.0 31 26712.0 32 34185.0 33 45003.0 34 61859.0 35 89648.0 36 137798.0 37 206811.0 38 154548.0 39 13291.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52810696499254 19.73627010578177 15.325881723581523 22.409741205644167 2 15.722135195961595 20.89029680191201 39.76226216957557 23.625305832550822 3 19.566249888343496 24.838070837064294 29.922853526275773 25.672825748316434 4 13.379825252257877 17.695796010714492 36.653126494402564 32.27125224262507 5 13.99380609347069 37.37041107887917 33.98391501515797 14.65186781249217 6 31.684656686372353 36.659008789674175 17.1359975773658 14.520336946587669 7 27.826742439344276 33.28126412704015 20.688250333057738 18.203743100557837 8 26.09495113882326 35.404772720093156 19.442837706384143 19.057438434699442 9 26.150179355540086 17.103209227796242 20.145445197160377 36.6011662195033 10-11 24.800042701129673 27.108409126930745 27.091742614583637 20.99980555735595 12-13 25.866225275216063 24.436005864885416 27.61893713112986 22.07883172876866 14-15 22.92521097287717 25.82872281208572 25.99239223144871 25.2536739835884 16-17 22.067212848675776 28.858268274058315 26.665206985988156 22.409311891277756 18-19 21.98556876893364 27.228327826851373 29.317740893607798 21.468362510607193 20-21 22.802085875135077 26.38030728204413 29.524098459232405 21.293508383588385 22-23 22.4362179455757 26.27730229224332 29.48130138157687 21.80517838060411 24-25 22.32175780554268 26.416866061609905 29.53412662836178 21.727249504485638 26-27 22.09339692111948 26.54575836931751 29.46347260772378 21.897372101839224 28-29 21.8771803786213 26.83699196667496 29.606580038856695 21.67924761584704 30-31 22.230490034955416 26.539533611836358 29.372191568973623 21.857784784234603 32-33 22.161783256318586 26.54868268834844 29.354328838809334 21.93520521652364 34-35 22.309356467784173 26.696467087002556 29.460912683715396 21.533263761497874 36-37 22.568597365856792 26.47297037473985 29.29943729925429 21.658994960149066 38-39 22.2570626823269 26.821907332727662 29.16402503720068 21.757004947744758 40-41 22.412935716304887 26.775259522073064 29.125242174304493 21.686562587317557 42-43 22.375407890429642 26.864149815002524 28.844246450515833 21.916195844052 44-45 22.393934690076062 26.892645212593578 28.881082126235352 21.832337971095008 46-47 22.629541354759436 27.2713309687933 28.32551568178055 21.773611994666712 48-49 22.59728531925358 27.354703142726894 28.14075250937219 21.90725902864734 50-51 22.57336257051946 27.510514602798015 28.014921422338997 21.90120140434353 52-53 22.794665115460806 27.65694385212128 27.680963577708166 21.867427454709745 54-55 22.669044360068042 27.712855394446372 27.67156893449887 21.94653131098672 56-57 22.507121420907296 27.905730360945746 27.52697425395286 22.060173964194092 58-59 22.414501168307016 27.555651174570666 27.904400350764437 22.125447306357877 60-61 22.600036928164915 27.623029885125085 27.885339681927583 21.891593504782414 62-63 22.35441046874268 27.516887089342262 28.149669556616548 21.979032885298512 64-65 22.365340152449733 27.600101306361875 27.952222612560355 22.082335928628034 66-67 22.32766781153562 27.789596834017967 27.80555528322707 22.077180071219345 68-69 22.358822190196392 27.667103481556282 27.82036995280003 22.1537043754473 70-71 22.433028906066177 27.693603977307387 27.71185000566444 22.161517110961995 72-73 22.365793799407196 27.720806658758246 27.56547049103004 22.347929050804513 74-75 22.36649611604269 27.960080914415684 27.412536069986395 22.26088689955523 76-77 22.39987799630721 27.954531838061886 27.389883497366576 22.255706668264335 78-79 22.47112494784898 28.020973696279007 27.171198040973543 22.33670331489847 80-81 22.55295947780201 27.922439393180138 27.21135641189325 22.3132447171246 82-83 22.469154361363337 28.141658183537338 27.29198359256578 22.097203862533544 84-85 22.428333094584357 28.324815532372998 26.949403554218804 22.29744781882384 86-87 22.361692312133407 27.939697069221793 27.15984830040572 22.538762318239083 88-89 22.342550295506697 27.955952045725514 27.15764006407411 22.543857594693677 90-91 22.166388709454285 28.09156139332271 27.12822451244925 22.613825384773754 92-93 22.207516484043186 27.990357393010306 27.213404225884496 22.588721897062012 94-95 22.123838857525673 28.089912119454365 27.163013374073742 22.623235648946224 96-97 22.07349899073964 28.119366143100372 27.218166904321244 22.588967961838737 98-99 21.879447592955202 28.160776485390514 26.984555805802398 22.975220115851883 100-101 21.624155027731376 28.620588047866917 26.884156759750915 22.871100164650787 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 329.0 1 233.0 2 178.0 3 212.5 4 188.5 5 192.0 6 324.5 7 695.5 8 1166.5 9 1407.5 10 1280.5 11 1116.5 12 1140.0 13 1223.5 14 1383.5 15 1456.0 16 1482.0 17 1541.0 18 1554.0 19 1683.5 20 1906.0 21 2121.5 22 2474.5 23 3038.5 24 3718.5 25 4611.0 26 5864.0 27 7538.0 28 8869.5 29 10003.0 30 11487.5 31 13072.5 32 14872.0 33 16547.0 34 18231.5 35 19855.0 36 21918.5 37 23916.5 38 24984.0 39 25832.0 40 26711.5 41 27712.0 42 28245.0 43 28842.0 44 30058.0 45 30982.5 46 31672.0 47 32909.0 48 34289.0 49 34251.0 50 33021.5 51 32320.5 52 32375.0 53 31988.0 54 31317.5 55 29646.5 56 26679.0 57 23800.5 58 22025.0 59 19949.0 60 16554.5 61 13432.5 62 10637.0 63 7902.5 64 5527.5 65 3886.5 66 2873.0 67 2290.0 68 1824.5 69 1548.5 70 1217.5 71 759.5 72 499.0 73 298.0 74 184.0 75 131.0 76 64.0 77 30.0 78 23.0 79 14.0 80 7.0 81 7.5 82 7.0 83 5.0 84 4.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019171925329708097 2 0.002505422060132308 3 0.00163397090878194 4 0.0 5 0.00108931393918796 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.4465696959398E-5 12-13 3.26794181756388E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0027232848479699 22-23 0.0 24-25 0.002559887757091706 26-27 0.001416108120944348 28-29 0.002233093575335318 30-31 0.013670889936808898 32-33 3.26794181756388E-4 34-35 0.001416108120944348 36-37 0.002777750544929298 38-39 0.00163397090878194 40-41 0.002559887757091706 42-43 0.002559887757091706 44-45 1.63397090878194E-4 46-47 1.08931393918796E-4 48-49 5.4465696959398E-5 50-51 0.0 52-53 0.001470573817903746 54-55 0.0035402703023608697 56-57 4.35725575675184E-4 58-59 0.001525039514863144 60-61 0.001143779636147358 62-63 0.0 64-65 1.63397090878194E-4 66-67 0.0 68-69 0.0 70-71 1.08931393918796E-4 72-73 0.0 74-75 2.17862787837592E-4 76-77 4.35725575675184E-4 78-79 2.17862787837592E-4 80-81 0.004684049938508229 82-83 0.001143779636147358 84-85 0.003213476120604482 86-87 0.001143779636147358 88-89 0.001034848242228562 90-91 2.17862787837592E-4 92-93 0.0 94-95 1.63397090878194E-4 96-97 0.00108931393918796 98-99 0.0028866819388480938 100-101 5.99122666553378E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 918009.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.04842214089004 #Duplication Level Percentage of deduplicated Percentage of total 1 82.23097386183649 54.312260746830724 2 10.74474189423059 14.193464968500969 3 3.061405725643835 6.066030531355855 4 1.3169254292672006 3.479233867212516 5 0.7037455593833464 2.3240641892964033 6 0.4358371605106024 1.7271814057254673 7 0.28895696698230167 1.3359606215058801 8 0.20454923212112797 1.0808123225384934 9 0.15265381403827216 0.9074289195915054 >10 0.8001401180971215 9.476047200115698 >50 0.04264929153609544 1.9269380572373196 >100 0.01659137757313028 1.903902442031773 >500 1.6591375599244647E-4 0.06355838240966444 >1k 6.636550239697859E-4 1.2031163456477687 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4106 0.44727230343057633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3596 0.3917172925319904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2268 0.24705640140782933 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.10991177646406518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.08931393918796E-4 0.0 20-21 0.0 0.0 0.0 1.63397090878194E-4 0.0 22-23 0.0 0.0 0.0 4.901912726345821E-4 0.0 24-25 0.0 0.0 0.0 9.259168483097659E-4 1.08931393918796E-4 26-27 0.0 0.0 0.0 0.001416108120944348 1.08931393918796E-4 28-29 2.7232848479699E-4 0.0 0.0 0.0029411476358074925 1.08931393918796E-4 30-31 3.2679418175638803E-4 0.0 0.0 0.005010844120264616 1.08931393918796E-4 32-33 3.2679418175638803E-4 0.0 0.0 0.007951991756072108 1.08931393918796E-4 34-35 3.2679418175638803E-4 0.0 0.0 0.010729742301001407 1.08931393918796E-4 36-37 3.2679418175638803E-4 0.0 0.0 0.014487875391199868 1.08931393918796E-4 38-39 3.2679418175638803E-4 0.0 0.0 0.018954062541870507 1.08931393918796E-4 40-41 3.2679418175638803E-4 0.0 0.0 0.02401937235909452 1.08931393918796E-4 42-43 3.2679418175638803E-4 0.0 0.0 0.03387766350874556 1.08931393918796E-4 44-45 3.2679418175638803E-4 0.0 0.0 0.047820881930351444 1.08931393918796E-4 46-47 3.81259878715786E-4 0.0 0.0 0.05920421259486563 1.08931393918796E-4 48-49 5.4465696959398E-4 0.0 0.0 0.07178578859248658 1.08931393918796E-4 50-51 5.4465696959398E-4 0.0 0.0 0.0845307616809857 1.08931393918796E-4 52-53 5.4465696959398E-4 0.0 0.0 0.11328864967554783 1.08931393918796E-4 54-55 5.4465696959398E-4 0.0 0.0 0.1392687871251807 1.08931393918796E-4 56-57 5.4465696959398E-4 0.0 0.0 0.18948615972174565 1.08931393918796E-4 58-59 5.4465696959398E-4 0.0 0.0 0.2625791250412578 1.08931393918796E-4 60-61 5.4465696959398E-4 0.0 0.0 0.3271754416351038 1.08931393918796E-4 62-63 5.4465696959398E-4 0.0 0.0 0.41540987070932855 1.08931393918796E-4 64-65 5.4465696959398E-4 0.0 0.0 0.4734703036680468 1.08931393918796E-4 66-67 5.4465696959398E-4 0.0 0.0 0.580386466799345 1.08931393918796E-4 68-69 5.4465696959398E-4 0.0 0.0 0.7048950500485289 1.08931393918796E-4 70-71 5.4465696959398E-4 0.0 0.0 0.7875195123359358 1.08931393918796E-4 72-73 5.99122666553378E-4 0.0 0.0 0.9536398880620996 1.08931393918796E-4 74-75 6.535883635127761E-4 0.0 0.0 1.1035839517913222 1.08931393918796E-4 76-77 6.535883635127761E-4 0.0 0.0 1.1965568965010147 1.08931393918796E-4 78-79 6.535883635127761E-4 0.0 0.0 1.2968282446032664 1.08931393918796E-4 80-81 6.535883635127761E-4 0.0 0.0 1.478743672447656 1.08931393918796E-4 82-83 6.535883635127761E-4 0.0 0.0 1.6300493786008634 1.08931393918796E-4 84-85 6.535883635127761E-4 0.0 0.0 1.8760709317664643 1.08931393918796E-4 86-87 6.535883635127761E-4 0.0 0.0 2.268496278358927 2.17862787837592E-4 88-89 6.535883635127761E-4 0.0 0.0 2.775245122869166 2.17862787837592E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2620 0.0 42.430595 1 GTATCAA 4195 0.0 34.88055 1 CTAGCGT 65 0.005846579 29.227585 4 TATCAAC 5155 0.0 28.28499 2 ATCAACG 5195 0.0 27.884354 3 TCAACGC 5245 0.0 27.527985 4 CAACGCA 5260 0.0 27.44948 5 AACGCAG 5470 0.0 26.395662 6 TACATGG 3405 0.0 23.43357 2 GTACATG 3595 0.0 23.39047 1 ACGCAGA 6130 0.0 23.321276 7 GTCTAGA 430 0.0 23.201468 1 CGCAGAG 6145 0.0 23.18706 8 GAGTACT 3605 0.0 22.726374 12-13 ACATGGG 3485 0.0 21.80537 3 GTACTTT 3855 0.0 21.129921 14-15 GCAGAGT 6675 0.0 20.563303 9 AGAGTAC 5600 0.0 20.31252 10-11 AGTACTT 3855 0.0 19.897314 12-13 CAGAGTA 6200 0.0 19.227743 10-11 >>END_MODULE