FastQCFastQC Report
Thu 26 May 2016
SRR937022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657720
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.554643313264003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26890.4088365869975065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.2563400839262908No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.11555069026333394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.048.1027571
GTATCAA29950.039.198451
ATCAACG35450.032.7146533
TATCAAC36250.032.254912
TCAACGC36300.031.817674
CAACGCA36500.031.5155055
AACGCAG37300.030.8348756
CGCAGAG40100.027.9749558
ACGCAGA40750.027.6432747
AGGATCG908.906475E-426.4076215
TACATGG20900.025.243262
GGATAGG1151.2754388E-424.7983151
GCAGAGT44550.024.5404199
GTACATG22900.024.0763841
GAGTACT28000.023.25668512-13
TGGTATC9150.022.8560052
ACATGGG21400.022.6545333
GTACTTT29700.021.83799414-15
GTACTAG1100.002865515221.6045911
CAGAGTA42300.021.51940310-11
Produced by FastQC (version 0.11.5)