FastQCFastQC Report
Thu 26 May 2016
SRR937022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657720
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.4573374688317217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.38177339901477836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.24022380344219424No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.1188955786656936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.044.2582551
GTATCAA31250.036.3347741
ATCAACG39300.028.6394923
TATCAAC39550.028.4627822
TCAACGC39700.028.3487764
CAACGCA40100.028.1844165
AACGCAG40750.027.6183176
GGGTACG350.008399934527.137684-85
TTGACGC700.00839693527.13763
GGACCAT3800.024.9932586
ACGCAGA45500.024.6307187
CGCAGAG45650.024.3417368
GTACCCG1302.3646862E-1123.74720648-49
TACATGG22250.023.2686962
CTCACGA1453.6379788E-1222.93967424-25
GAGTACT28250.022.60980412-13
GTACATG23700.022.0505261
ACATGGG22750.021.918833
GTCTAGA3059.094947E-1221.8073461
GTACTTT29650.021.62312914-15
Produced by FastQC (version 0.11.5)