##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937022_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 657720 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.261991424922456 33.0 31.0 34.0 31.0 34.0 2 32.3305981268625 33.0 31.0 34.0 31.0 34.0 3 32.46603721948549 34.0 31.0 34.0 31.0 34.0 4 36.12652800583835 37.0 35.0 37.0 35.0 37.0 5 36.04080763850879 37.0 35.0 37.0 35.0 37.0 6 36.06952198503922 37.0 35.0 37.0 35.0 37.0 7 36.03167609317035 37.0 35.0 37.0 35.0 37.0 8 36.01683999270206 37.0 35.0 37.0 35.0 37.0 9 37.720417502888765 39.0 37.0 39.0 35.0 39.0 10-11 37.624713403880065 39.0 37.0 39.0 35.0 39.0 12-13 37.551580459770115 39.0 37.0 39.0 35.0 39.0 14-15 38.81021863406921 40.0 38.0 41.0 35.0 41.0 16-17 38.73521863406921 40.0 38.0 41.0 35.0 41.0 18-19 38.76037827647023 40.0 38.0 41.0 34.5 41.0 20-21 38.8317597153804 40.0 38.0 41.0 35.0 41.0 22-23 38.766436325488044 40.0 38.0 41.0 35.0 41.0 24-25 38.68149592531776 40.0 38.0 41.0 34.0 41.0 26-27 38.51190248129903 40.0 38.0 41.0 34.0 41.0 28-29 38.3621601897464 40.0 38.0 41.0 34.0 41.0 30-31 38.3237760749255 40.0 38.0 41.0 34.0 41.0 32-33 38.24307988201666 40.0 38.0 41.0 34.0 41.0 34-35 38.12828635285531 40.0 38.0 41.0 34.0 41.0 36-37 37.982868089764644 40.0 37.0 41.0 33.0 41.0 38-39 37.83886076141823 40.0 37.0 41.0 33.0 41.0 40-41 37.6249133369823 40.0 37.0 41.0 33.0 41.0 42-43 37.420745149911816 40.0 37.0 41.0 32.5 41.0 44-45 37.17878656571185 40.0 37.0 41.0 32.0 41.0 46-47 36.84162561576355 40.0 36.0 41.0 31.0 41.0 48-49 36.48167077175698 39.0 36.0 41.0 30.0 41.0 50-51 36.247071702244114 39.0 35.5 41.0 30.0 41.0 52-53 36.022767286991424 39.0 35.0 40.5 29.5 41.0 54-55 35.76215182752539 39.0 35.0 40.0 29.0 41.0 56-57 35.50077768655355 38.0 35.0 40.0 28.0 41.0 58-59 35.16327768655355 38.0 34.5 40.0 27.5 41.0 60-61 34.8241394514383 38.0 34.0 40.0 27.0 41.0 62-63 34.4793513957307 37.0 34.0 40.0 26.0 41.0 64-65 34.06606458675424 37.0 33.5 39.5 26.0 41.0 66-67 33.63848446147297 36.0 33.0 39.0 25.5 40.0 68-69 33.19366219667944 36.0 32.5 39.0 24.5 40.0 70-71 32.81538876725658 35.0 32.0 38.0 24.0 40.0 72-73 32.42581645685094 35.0 32.0 37.5 22.5 40.0 74-75 31.948503922641855 35.0 31.5 37.0 21.5 39.0 76-77 30.490469348659005 33.5 29.5 35.5 20.0 38.0 78-79 31.18452456972572 34.0 31.0 36.0 20.0 38.5 80-81 30.854511798333636 34.0 31.0 36.0 18.0 37.5 82-83 30.708477011494253 34.0 31.0 35.0 18.0 37.0 84-85 30.402111840904944 34.0 31.0 35.0 17.5 36.5 86-87 30.054920026759106 34.0 30.0 35.0 13.5 36.0 88-89 29.709066928176124 34.0 30.0 35.0 7.5 36.0 90-91 29.2827464574591 34.0 29.5 35.0 4.5 35.0 92-93 28.881470078452836 34.0 29.0 35.0 2.0 35.0 94-95 28.402726844249834 34.0 29.0 35.0 2.0 35.0 96-97 27.820136988384114 33.0 28.5 35.0 2.0 35.0 98-99 27.116881043605183 33.0 27.0 35.0 2.0 35.0 100-101 23.991791339779844 29.5 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19203746983465209 1101 2 0.1195672171074662 1101 3 0.11317614251552044 1101 4 0.08481820310561972 1101 5 0.1832790232010737 1101 6 0.22807860047847583 1101 7 0.1036933994654774 1101 8 0.19619889679276525 1101 9 0.2711515926183594 1101 10-11 0.19277703831743764 1101 12-13 0.17321565790431492 1101 14-15 0.21823593993533308 1101 16-17 0.2527037693919141 1101 18-19 0.330413789026089 1101 20-21 0.19856882026897438 1101 22-23 0.2972959897619063 1101 24-25 0.2808439640107494 1101 26-27 0.31835481752826666 1101 28-29 0.3383180063573832 1101 30-31 0.2253692037802537 1101 32-33 0.22182013877836226 1101 34-35 0.22834291025147735 1101 36-37 0.3112492596158489 1101 38-39 0.30235854875871127 1101 40-41 0.22219919265749155 1101 42-43 0.20592869328917374 1101 44-45 0.03563112194159146 1101 46-47 0.03875883553393322 1101 48-49 -0.05805374059269752 1101 50-51 -0.1973045929775381 1101 52-53 0.015986170319287396 1101 54-55 -0.010035669049365481 1101 56-57 0.12333524575716837 1101 58-59 0.005866556703587378 1101 60-61 0.05516891763840448 1101 62-63 -0.06435696748917508 1101 64-65 0.054625750692089525 1101 66-67 -0.08501078009680185 1101 68-69 0.007073718406658713 1101 70-71 -0.01216416279903143 1101 72-73 0.12237972256626506 1101 74-75 0.12818354800215204 1101 76-77 -0.07083432144993651 1101 78-79 0.06892397818689844 1101 80-81 0.07443116005637407 1101 82-83 0.03743785454814841 1101 84-85 -0.07551910209767954 1101 86-87 -0.12371371284906374 1101 88-89 -0.22114991629817737 1101 90-91 -0.34392254335298134 1101 92-93 -0.2888921819647514 1101 94-95 -0.40464138057523513 1101 96-97 -0.392752552664021 1101 98-99 0.1413576056274799 1101 100-101 0.10621056708656695 1102 1 0.04055977892272722 1102 2 0.06844283308132049 1102 3 0.02363907454765979 1102 4 4.259583040209236E-4 1102 5 0.0737654545974209 1102 6 0.027567247781867366 1102 7 0.028876431210029807 1102 8 0.05890654878173507 1102 9 0.1130744855846757 1102 10-11 0.10389038703126374 1102 12-13 0.01594885031403237 1102 14-15 0.06743724049655242 1102 16-17 0.16173932255564694 1102 18-19 0.17613883013637377 1102 20-21 -0.01336394460744117 1102 22-23 -0.007351427927389409 1102 24-25 0.11158118682676132 1102 26-27 0.2741227412362903 1102 28-29 0.1188802766109589 1102 30-31 0.30413323519346847 1102 32-33 0.28088287201159545 1102 34-35 0.1919012065679837 1102 36-37 0.21969936973084714 1102 38-39 0.007609915075974527 1102 40-41 0.12581880231223153 1102 42-43 0.07891476579974466 1102 44-45 0.1238319782328432 1102 46-47 0.10043003901569847 1102 48-49 0.2919779298011633 1102 50-51 0.11671467595683538 1102 52-53 0.16680119821032235 1102 54-55 0.2072681360923312 1102 56-57 0.14879742600015078 1102 58-59 0.29685794548607447 1102 60-61 0.19971075827679385 1102 62-63 0.22870167138464836 1102 64-65 0.07941846439241118 1102 66-67 0.351087821983576 1102 68-69 0.30816085315109376 1102 70-71 0.2839825946084744 1102 72-73 0.37138998023604586 1102 74-75 0.31596721233816183 1102 76-77 0.2704061782823466 1102 78-79 0.27392667578327945 1102 80-81 0.4418601159811182 1102 82-83 0.3847885971865672 1102 84-85 0.31137540997755053 1102 86-87 0.16286593223841805 1102 88-89 0.2935712039425056 1102 90-91 0.2517274889297312 1102 92-93 0.2396281813160961 1102 94-95 0.2946671094715505 1102 96-97 0.1987514134489956 1102 98-99 0.3960028802074156 1102 100-101 0.49644186641635457 1103 1 0.08791676105594348 1103 2 0.013052786845292985 1103 3 0.04435438542349601 1103 4 0.09252801192904059 1103 5 0.10981968805783282 1103 6 0.10682717902621874 1103 7 0.13429383901434022 1103 8 0.1562771888597183 1103 9 0.10478836123434121 1103 10-11 0.1596741840495426 1103 12-13 0.13569796437477066 1103 14-15 0.13748057135409653 1103 16-17 0.16429870264026647 1103 18-19 0.2206381499798553 1103 20-21 0.0651696945907716 1103 22-23 0.12013383839340008 1103 24-25 0.13514274030607254 1103 26-27 0.041912100098564053 1103 28-29 0.23201602196475335 1103 30-31 0.20504233357726065 1103 32-33 0.1370962319482274 1103 34-35 0.13579309045992005 1103 36-37 0.20018906414918547 1103 38-39 -0.02209817033597261 1103 40-41 -0.07867397633496154 1103 42-43 0.04926880348106266 1103 44-45 0.05607121426496775 1103 46-47 0.035209013272073264 1103 48-49 -0.09713554625083987 1103 50-51 -0.03607752869125136 1103 52-53 -0.03251983292339844 1103 54-55 0.053156272500025636 1103 56-57 0.07603392587544278 1103 58-59 0.09531738413084412 1103 60-61 0.17689422512909658 1103 62-63 -0.04878254432500029 1103 64-65 -0.04627162724828793 1103 66-67 -0.185668950575554 1103 68-69 -0.280036581347872 1103 70-71 6.624624324587103E-4 1103 72-73 0.11618009097045956 1103 74-75 -0.25250751041469854 1103 76-77 -0.07671528424144114 1103 78-79 0.019321573102033085 1103 80-81 0.47036273931144734 1103 82-83 0.36357767192704316 1103 84-85 0.19216804583299307 1103 86-87 0.1788260857555457 1103 88-89 -0.2204677015582206 1103 90-91 -0.21918017514146726 1103 92-93 -0.09626635925165061 1103 94-95 0.04415710628317626 1103 96-97 0.17948274842859036 1103 98-99 0.3994278725105467 1103 100-101 0.3171157923491812 1104 1 0.1628620290016869 1104 2 0.13959484075491702 1104 3 0.1866126795337948 1104 4 0.14574262722157982 1104 5 0.13399832112719423 1104 6 0.14876461008828556 1104 7 0.15509523727192231 1104 8 0.0964844306087258 1104 9 0.1800759860626826 1104 10-11 0.18372411152393653 1104 12-13 0.19588391821837092 1104 14-15 0.22095708966183025 1104 16-17 0.26248562692404676 1104 18-19 0.102873817646568 1104 20-21 -0.03170571968036029 1104 22-23 0.10615319314076999 1104 24-25 0.1501794149653577 1104 26-27 0.1798388074104551 1104 28-29 0.05294857122359531 1104 30-31 0.32760545401867347 1104 32-33 0.32895187060469055 1104 34-35 0.13756922767039015 1104 36-37 0.2509531249136643 1104 38-39 0.14098018434318504 1104 40-41 0.12176825608931807 1104 42-43 0.11930910120283755 1104 44-45 0.19415558593068027 1104 46-47 0.34325491483546244 1104 48-49 0.5723961069596513 1104 50-51 0.473807704529726 1104 52-53 0.3700255776281409 1104 54-55 0.20814226289601123 1104 56-57 0.3616850539156289 1104 58-59 0.3403393734817399 1104 60-61 0.25664278587878897 1104 62-63 0.3629402186752273 1104 64-65 0.2422921084058629 1104 66-67 0.2793668874973676 1104 68-69 0.31354332616429303 1104 70-71 0.14060984758523887 1104 72-73 0.3275629424944029 1104 74-75 0.08790784828136466 1104 76-77 0.05783225767475031 1104 78-79 0.49744419607057466 1104 80-81 0.6149776328654859 1104 82-83 0.4426691906144562 1104 84-85 0.5764224607566639 1104 86-87 0.22094113053865883 1104 88-89 0.1731230172239151 1104 90-91 0.35104031041326067 1104 92-93 0.5621830987047325 1104 94-95 0.6803372786714483 1104 96-97 1.1301505374080492 1104 98-99 1.3361613502515581 1104 100-101 1.5938527708823749 1105 1 0.20819199001345368 1105 2 0.09814338813417578 1105 3 0.04944620840083758 1105 4 0.12952771959644593 1105 5 0.2235239783659182 1105 6 0.18273330099436436 1105 7 0.13252675391937174 1105 8 0.1290073571473087 1105 9 0.104688008253639 1105 10-11 0.24564396471282635 1105 12-13 0.18241664006448133 1105 14-15 0.35971661029105206 1105 16-17 0.4418205075977042 1105 18-19 0.30933709540092735 1105 20-21 0.16852671995389557 1105 22-23 0.07159790111838049 1105 24-25 0.19357435411289003 1105 26-27 0.17708102034411866 1105 28-29 0.19959546443848808 1105 30-31 0.23488861898986357 1105 32-33 0.36433246722797463 1105 34-35 0.40082531249307607 1105 36-37 0.3326091853581232 1105 38-39 0.3535710313342548 1105 40-41 0.4427601113909887 1105 42-43 0.6912748159335536 1105 44-45 0.7220855865570712 1105 46-47 0.5651687411845998 1105 48-49 0.9385333936289939 1105 50-51 0.6672768787680283 1105 52-53 0.5660180677795097 1105 54-55 0.4888736436009822 1105 56-57 0.29886117476392116 1105 58-59 0.3407676854079469 1105 60-61 0.4459972876402887 1105 62-63 0.7834883563648205 1105 64-65 0.7840838404620456 1105 66-67 0.664303343275428 1105 68-69 0.6010320470014108 1105 70-71 0.8124111785687802 1105 72-73 0.9392076095030433 1105 74-75 0.6368446020155467 1105 76-77 0.4078739884612368 1105 78-79 0.6799888767919668 1105 80-81 0.6951752319485927 1105 82-83 0.9121506961731995 1105 84-85 0.7508309078127375 1105 86-87 0.6589671035092941 1105 88-89 0.5830717044393943 1105 90-91 0.6306721314499768 1105 92-93 0.6501089347171352 1105 94-95 0.6658525480762627 1105 96-97 0.7217051742655585 1105 98-99 0.9770683557755966 1105 100-101 1.4102957165172363 1106 1 0.04178837182407591 1106 2 0.046853177780249666 1106 3 0.054738998326925525 1106 4 0.07459725773423287 1106 5 0.13223554995328612 1106 6 0.031177072260980765 1106 7 0.04663506200854073 1106 8 0.02584780551767807 1106 9 0.19511304368032967 1106 10-11 0.17124579417256314 1106 12-13 0.0878761687834313 1106 14-15 0.21761739858794016 1106 16-17 0.08038235368888991 1106 18-19 0.14988198759348137 1106 20-21 -0.001239251755656312 1106 22-23 0.12699294053322063 1106 24-25 0.09851839267071227 1106 26-27 0.17386716193208684 1106 28-29 0.12848115714695751 1106 30-31 0.10675219016993509 1106 32-33 0.27035521667853857 1106 34-35 0.32501431706672435 1106 36-37 0.34151995252628353 1106 38-39 0.36081971397049983 1106 40-41 0.4748997561906947 1106 42-43 0.49345493625701664 1106 44-45 0.42308962562401575 1106 46-47 0.8573965072241947 1106 48-49 1.0344824470787515 1106 50-51 0.6046066397333334 1106 52-53 0.5715972411284582 1106 54-55 0.8293952008479692 1106 56-57 0.7835608752280763 1106 58-59 0.8085728852823593 1106 60-61 0.747620551308195 1106 62-63 0.7023262336484564 1106 64-65 0.970126997383197 1106 66-67 0.9141484840912142 1106 68-69 0.5961592860954994 1106 70-71 0.7011851605287447 1106 72-73 0.7616307951049563 1106 74-75 0.7218559300709408 1106 76-77 0.35889818043571253 1106 78-79 0.5226720985418325 1106 80-81 0.9945816757877459 1106 82-83 0.8114901050778123 1106 84-85 0.477178819122976 1106 86-87 0.7774661998104939 1106 88-89 0.6495593284328116 1106 90-91 0.7737768671711116 1106 92-93 1.0142146971758699 1106 94-95 1.1289249967181725 1106 96-97 1.1465242223694858 1106 98-99 1.0564655080636918 1106 100-101 1.0162692504462463 1107 1 0.1865978013202323 1107 2 0.05505084939031235 1107 3 -0.02781498457245135 1107 4 0.041811401978471 1107 5 0.17260316504435025 1107 6 0.060371314085045924 1107 7 0.06738043662812032 1107 8 0.10984616308494566 1107 9 0.15444351080752483 1107 10-11 0.0022998495322070767 1107 12-13 0.19053787594733507 1107 14-15 0.05648750148152004 1107 16-17 0.041911989938341776 1107 18-19 0.15403275325697052 1107 20-21 0.17663379785546596 1107 22-23 0.15627896536294372 1107 24-25 0.11051056696180694 1107 26-27 0.24497725323202246 1107 28-29 0.26416783894977414 1107 30-31 0.08849944875647964 1107 32-33 0.13809228252076622 1107 34-35 0.3046795506303184 1107 36-37 0.2860141798582987 1107 38-39 0.21995453837512002 1107 40-41 0.3617252718662627 1107 42-43 0.26403485993220244 1107 44-45 -0.1668396048479437 1107 46-47 0.019729244773103005 1107 48-49 0.17103140606624123 1107 50-51 0.08062349882226982 1107 52-53 0.148864039833839 1107 54-55 0.036644850623339664 1107 56-57 -0.00596016240294972 1107 58-59 0.17602977142312426 1107 60-61 0.2580065230003825 1107 62-63 0.09119029818639746 1107 64-65 0.01925988951401081 1107 66-67 -0.19610222843474645 1107 68-69 -0.22116113320791442 1107 70-71 0.29327612231803357 1107 72-73 0.27936182259155373 1107 74-75 0.17020625131145906 1107 76-77 0.07012227589003928 1107 78-79 0.20004637997751473 1107 80-81 0.21353486645486086 1107 82-83 0.4440126885278879 1107 84-85 0.18858044223296844 1107 86-87 0.30784154399676567 1107 88-89 0.16823958542522988 1107 90-91 -0.2828073558132864 1107 92-93 -0.08990587670944805 1107 94-95 -0.1285082190685074 1107 96-97 0.2913384825501524 1107 98-99 0.44479578196350644 1107 100-101 0.10825152183410225 1108 1 0.04629569622622398 1108 2 -0.11919790522964746 1108 3 -0.12673297696625951 1108 4 -0.12612474885981584 1108 5 0.019844581295885177 1108 6 0.10225090056530917 1108 7 -2.744993620211744E-4 1108 8 -0.06551941621764001 1108 9 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68-69 -0.9896623889391378 2316 70-71 -0.780847770538152 2316 72-73 -0.854037053059777 2316 74-75 -0.8290796353420369 2316 76-77 -0.8579444342728912 2316 78-79 -0.6369981381523857 2316 80-81 -0.5394757603382416 2316 82-83 -0.8942768357242592 2316 84-85 -1.364242495194972 2316 86-87 -1.0675648928547012 2316 88-89 -0.9596340728192523 2316 90-91 -1.0172563981506428 2316 92-93 -1.096141632427269 2316 94-95 -0.9214421613495567 2316 96-97 -0.78134288347092 2316 98-99 -1.0197210966729777 2316 100-101 -1.495388758091181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 8.0 6 23.0 7 64.0 8 186.0 9 363.0 10 589.0 11 888.0 12 1362.0 13 1853.0 14 2299.0 15 3258.0 16 4219.0 17 5885.0 18 5753.0 19 3805.0 20 3280.0 21 3297.0 22 3464.0 23 3754.0 24 4249.0 25 4946.0 26 6178.0 27 7297.0 28 9435.0 29 11541.0 30 14469.0 31 18171.0 32 23193.0 33 30972.0 34 42678.0 35 62741.0 36 98986.0 37 151966.0 38 116948.0 39 9593.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63192071450622 19.7813778850409 15.270852057490195 22.315849342962682 2 15.715152409031264 21.063571299327474 39.792540647223305 23.42873564441795 3 19.53787390368217 24.934464218375567 30.095216952327657 25.432444925614604 4 13.38046585173022 17.87569178373776 36.81049686796813 31.933345496563888 5 13.933987538277679 37.42272242106511 34.05626324704194 14.587026793615268 6 31.474639664294834 36.971507632427176 17.086450161162805 14.467402542115185 7 27.578452837073524 33.49875326886821 20.82846804111172 18.094325852946543 8 26.002177224889696 35.306620770603814 19.50760052180418 19.183601482702315 9 26.14486407589856 17.22359058565955 20.27777777777778 36.35376756066411 10-11 24.678662652800583 27.212187557015145 27.186036611323967 20.923113178860305 12-13 25.730249954387887 24.60393480508423 27.726084047923127 21.939731192604757 14-15 22.79412211883476 25.952761053335767 26.121145776318187 25.131971051511282 16-17 21.89100225019765 29.037888463175822 26.73911390865414 22.331995377972387 18-19 21.865043435423875 27.49404934312806 29.368534766204373 21.272372455243683 20-21 22.725417111075803 26.552872587171976 29.53405650071422 21.187653801037996 22-23 22.329866812625436 26.497825822538466 29.512938636501858 21.659368728334247 24-25 22.279992063087807 26.544333812538344 29.587196939575318 21.58847718479853 26-27 21.846136769849846 26.83228888147226 29.579363263220877 21.74221108545702 28-29 21.720223120848512 27.003339818343687 29.69959881125883 21.576838249548977 30-31 22.07492291372801 26.824577878996415 29.44273564066201 21.657763566613564 32-33 22.064977843797443 26.749671020872974 29.45075879957398 21.734592335755604 34-35 22.10800159344619 26.95873765003178 29.612099243721246 21.321161512800785 36-37 22.409300720089593 26.7800947802438 29.366213192442824 21.444391307223782 38-39 22.201830538008487 27.007543993960546 29.244893568332714 21.545731899698257 40-41 22.189328193958193 26.92675250031361 29.33328771757765 21.550631588150548 42-43 22.240012407513845 27.131172966216894 28.792426550863205 21.836388075406056 44-45 22.190089995575484 27.24308536275713 28.899466169174655 21.667358472492737 46-47 22.45076067422222 27.48568509333958 28.406765332838685 21.656788899599515 48-49 22.382703041305376 27.70548158689759 28.116528384047097 21.79528698774994 50-51 22.483856695195247 27.786250024707197 28.054380168556325 21.67551311154123 52-53 22.592619904318294 27.87510747656802 27.786237373641388 21.7460352454723 54-55 22.521628073162944 27.92689787292272 27.748779857383955 21.802694196530386 56-57 22.340915621764378 28.08852439575076 27.66813082222293 21.902429160261935 58-59 22.26606093225931 27.822655439902448 27.927868343991463 21.983415283846778 60-61 22.387355095903345 27.86628646406257 27.997801494406048 21.748556945628035 62-63 22.1489555958125 27.813984532920188 28.196822505642604 21.840237365624706 64-65 22.151999300558415 27.940638696605806 27.94147498545998 21.965887017375803 66-67 22.110919115998076 28.07149313492881 27.820015857968556 21.997571891104563 68-69 22.208589143609533 27.905670245729908 27.917605524589717 21.968135086070845 70-71 22.176296183670367 28.043484871521972 27.79435913030257 21.985859814505094 72-73 22.161686676089126 27.985846542643984 27.58776154739196 22.264705233874935 74-75 22.11041599670359 28.315585488808857 27.498705688357123 22.075292826130422 76-77 22.188958204412035 28.190469529467038 27.501782724976852 22.118789541144075 78-79 22.22239117229131 28.298891594624248 27.13681078192035 22.341906451164096 80-81 22.425272152283647 28.147692027002375 27.188773338198622 22.238262482515356 82-83 22.2110805665874 28.45952208632612 27.309059559793948 22.020337787292533 84-85 22.180816369350342 28.676771316538876 26.972923004878997 22.169489309231782 86-87 22.024417685337223 28.120932919783492 27.235145654685883 22.619503740193394 88-89 21.95110640795239 28.291324192582184 27.21866951288858 22.53889988657684 90-91 21.90485240567723 28.237534494423876 27.29419277346571 22.56342032643318 92-93 21.926841097794043 28.134659330200286 27.38205829541187 22.5564412765938 94-95 21.71277442484718 28.40099039631817 27.263494225855666 22.62274095297898 96-97 21.786186968760997 28.423286826679156 27.332472277315784 22.45805392724406 98-99 21.455428398536146 28.644234526399654 26.94494744700228 22.955389628061916 100-101 21.203517030198533 29.022036933986506 26.8360564014146 22.938389634400362 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 226.0 1 160.5 2 112.5 3 146.5 4 141.5 5 147.0 6 224.5 7 470.0 8 819.5 9 1016.5 10 991.5 11 872.5 12 873.5 13 935.5 14 985.0 15 1037.0 16 1080.0 17 1137.0 18 1161.0 19 1232.0 20 1398.5 21 1627.5 22 1863.0 23 2251.0 24 2814.5 25 3530.0 26 4450.5 27 5684.0 28 6608.0 29 7446.0 30 8665.5 31 9919.0 32 11160.5 33 12234.0 34 13460.0 35 14709.0 36 16318.0 37 17642.0 38 18401.0 39 19069.0 40 19737.5 41 20335.5 42 20303.5 43 20720.5 44 21554.5 45 22302.5 46 22973.0 47 23462.5 48 24327.5 49 24334.5 50 23383.5 51 22515.0 52 22505.0 53 22576.0 54 22145.0 55 20834.5 56 18323.0 57 16396.5 58 15221.5 59 13610.0 60 11246.0 61 9203.0 62 7284.5 63 5244.0 64 3663.5 65 2612.0 66 1916.0 67 1509.5 68 1221.5 69 1053.5 70 846.5 71 537.5 72 351.5 73 208.5 74 129.5 75 91.5 76 58.0 77 27.0 78 13.5 79 7.5 80 7.5 81 9.0 82 5.0 83 3.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02234993614303959 2 0.007449978714346531 3 0.009730584443228121 4 0.0 5 0.003953049930061425 6 0.0 7 0.0 8 3.040807638508788E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.002964787447546068 20-21 0.001748464392142553 22-23 0.0 24-25 0.00509335279450222 26-27 0.005853554704129417 28-29 0.007525998905309249 30-31 0.026987167791765493 32-33 5.321413367390379E-4 34-35 0.003953049930061425 36-37 0.014215775710028583 38-39 0.0067657969956820534 40-41 0.007982120051085569 42-43 0.008210180623973728 44-45 0.0031928480204342275 46-47 0.0021285653469561515 48-49 0.0013683634373289546 50-51 0.00258468649273247 52-53 0.002812747065620629 54-55 0.001520403819254394 56-57 4.561211457763182E-4 58-59 9.122422915526364E-4 60-61 7.60201909627197E-5 62-63 7.60201909627197E-5 64-65 0.007982120051085569 66-67 9.882624825153562E-4 68-69 9.122422915526364E-4 70-71 0.003040807638508788 72-73 0.010870887307668917 74-75 0.005245393176427659 76-77 0.0028887672565833483 78-79 0.0096545642522654 80-81 0.0 82-83 0.004257130693912303 84-85 4.561211457763182E-4 86-87 0.0 88-89 9.122422915526364E-4 90-91 7.60201909627197E-4 92-93 1.520403819254394E-4 94-95 5.321413367390379E-4 96-97 7.60201909627197E-5 98-99 4.561211457763182E-4 100-101 9.122422915526364E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 657720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.27112959505537 #Duplication Level Percentage of deduplicated Percentage of total 1 84.25571308956982 58.364884205513945 2 9.654834168064182 13.376025377494852 3 2.6174036444285336 5.439315211673375 4 1.1557342782452837 3.202360758631073 5 0.6230757855033533 2.1580581742571856 6 0.3666249254002689 1.5237913632107725 7 0.26449767429128634 1.282543687139571 8 0.1759472152565929 0.9750449879942841 9 0.1439196960938947 0.8972531927461048 >10 0.6952955869419252 8.433110683479738 >50 0.03269231717341084 1.5457236175279747 >100 0.013383980937249683 1.6040756043472653 >500 2.1940952354247565E-4 0.11885419012045544 >1k 6.58228570627427E-4 1.078958945863316 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.4573374688317217 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2511 0.38177339901477836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1580 0.24022380344219424 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 782 0.1188955786656936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.520403819254394E-4 0.0 5 0.0 0.0 0.0 1.520403819254394E-4 0.0 6 0.0 0.0 0.0 1.520403819254394E-4 0.0 7 0.0 0.0 0.0 1.520403819254394E-4 0.0 8 0.0 0.0 0.0 3.040807638508788E-4 0.0 9 0.0 0.0 0.0 3.040807638508788E-4 0.0 10-11 1.520403819254394E-4 0.0 0.0 3.040807638508788E-4 0.0 12-13 1.520403819254394E-4 0.0 0.0 4.561211457763182E-4 0.0 14-15 1.520403819254394E-4 0.0 0.0 4.561211457763182E-4 0.0 16-17 1.520403819254394E-4 0.0 0.0 4.561211457763182E-4 0.0 18-19 1.520403819254394E-4 0.0 0.0 6.081615277017576E-4 0.0 20-21 1.520403819254394E-4 0.0 0.0 8.362221005899167E-4 0.0 22-23 1.520403819254394E-4 0.0 0.0 0.0012923432463662348 0.0 24-25 1.520403819254394E-4 0.0 0.0 0.002280605728881591 0.0 26-27 1.520403819254394E-4 0.0 0.0 0.0028887672565833487 0.0 28-29 3.040807638508788E-4 0.0 0.0 0.004333150884875023 0.0 30-31 3.040807638508788E-4 0.0 0.0 0.008134160433011007 0.0 32-33 3.040807638508788E-4 0.0 0.0 0.012467311317886032 0.0 34-35 3.040807638508788E-4 0.0 0.0 0.01512801800158122 0.0 36-37 3.040807638508788E-4 0.0 0.0 0.019081067931642645 0.0 38-39 3.040807638508788E-4 0.0 0.0 0.025618804354436538 1.520403819254394E-4 40-41 3.040807638508788E-4 0.0 0.0 0.030103995621237 1.520403819254394E-4 42-43 3.040807638508788E-4 0.0 0.0 0.04409171075837742 1.520403819254394E-4 44-45 3.040807638508788E-4 0.0 0.0 0.06165237487076568 1.520403819254394E-4 46-47 3.040807638508788E-4 0.0 0.0 0.07343550446998723 1.520403819254394E-4 48-49 3.040807638508788E-4 0.0 0.0 0.08689107827038861 1.520403819254394E-4 50-51 3.040807638508788E-4 0.0 0.0 0.10437572219181415 1.520403819254394E-4 52-53 3.040807638508788E-4 0.0 0.0 0.1451985647387946 1.520403819254394E-4 54-55 3.040807638508788E-4 0.0 0.0 0.1783433679985404 1.520403819254394E-4 56-57 6.081615277017576E-4 0.0 0.0 0.24372073222647933 1.520403819254394E-4 58-59 6.081615277017576E-4 0.0 0.0 0.3369975065377364 1.520403819254394E-4 60-61 6.081615277017576E-4 0.0 0.0 0.41780696953110746 1.520403819254394E-4 62-63 6.081615277017576E-4 0.0 0.0 0.5460530316852157 1.520403819254394E-4 64-65 7.60201909627197E-4 0.0 0.0 0.6213890409292708 1.520403819254394E-4 66-67 7.60201909627197E-4 0.0 0.0 0.7709207565529405 1.520403819254394E-4 68-69 7.60201909627197E-4 0.0 0.0 0.9415860852642461 1.520403819254394E-4 70-71 7.60201909627197E-4 0.0 0.0 1.0431490603904399 1.520403819254394E-4 72-73 7.60201909627197E-4 0.0 0.0 1.258970382533601 1.520403819254394E-4 74-75 7.60201909627197E-4 0.0 0.0 1.4800370978531898 1.520403819254394E-4 76-77 7.60201909627197E-4 0.0 0.0 1.6042540898862736 1.520403819254394E-4 78-79 7.60201909627197E-4 0.0 0.0 1.7256583348537373 1.520403819254394E-4 80-81 7.60201909627197E-4 0.0 0.0 1.9683147844067386 1.520403819254394E-4 82-83 7.60201909627197E-4 0.0 0.0 2.17911877394636 1.520403819254394E-4 84-85 7.60201909627197E-4 0.0 0.0 2.501292343246366 1.520403819254394E-4 86-87 7.60201909627197E-4 0.0 0.0 3.000592957489509 1.520403819254394E-4 88-89 7.60201909627197E-4 0.0 0.0 3.688955786656936 1.520403819254394E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1900 0.0 44.258255 1 GTATCAA 3125 0.0 36.334774 1 ATCAACG 3930 0.0 28.639492 3 TATCAAC 3955 0.0 28.462782 2 TCAACGC 3970 0.0 28.348776 4 CAACGCA 4010 0.0 28.184416 5 AACGCAG 4075 0.0 27.618317 6 GGGTACG 35 0.0083999345 27.1376 84-85 TTGACGC 70 0.008396935 27.1376 3 GGACCAT 380 0.0 24.993258 6 ACGCAGA 4550 0.0 24.630718 7 CGCAGAG 4565 0.0 24.341736 8 GTACCCG 130 2.3646862E-11 23.747206 48-49 TACATGG 2225 0.0 23.268696 2 CTCACGA 145 3.6379788E-12 22.939674 24-25 GAGTACT 2825 0.0 22.609804 12-13 GTACATG 2370 0.0 22.050526 1 ACATGGG 2275 0.0 21.91883 3 GTCTAGA 305 9.094947E-12 21.807346 1 GTACTTT 2965 0.0 21.623129 14-15 >>END_MODULE