FastQCFastQC Report
Thu 26 May 2016
SRR937021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700046
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36300.518537353259643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27690.39554543558566146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.2412698594092388No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.10656442576630679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.045.2642251
GTATCAA33200.040.0728341
AATACCG756.9001053E-638.0146685
ATACCGT801.0741611E-535.638756
TATCAAC40800.032.727092
ATCAACG40850.032.338063
TCAACGC41750.031.98244
CAACGCA42000.031.7920285
AACGCAG43550.030.5513946
ACGCAGA47550.027.279877
CGCAGAG47800.027.0377888
TACCGTG1109.451209E-525.917247
GCAGAGT50300.024.8437929
GTACATG25000.023.9475291
GAGTACT31300.023.3754512-13
TACATGG24700.023.085832
GAGTACG1050.00219136122.6261621
AGAGTAC44100.021.3855810-11
AGTACTT32950.021.33977712-13
GTACTTT34450.021.30626714-15
Produced by FastQC (version 0.11.5)