##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937021_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700046 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81793482142602 33.0 31.0 34.0 30.0 34.0 2 31.943436574167983 33.0 31.0 34.0 30.0 34.0 3 31.934691434562872 33.0 31.0 34.0 30.0 34.0 4 35.49183196532799 37.0 35.0 37.0 33.0 37.0 5 35.48331395365447 37.0 35.0 37.0 33.0 37.0 6 35.58019901549327 37.0 35.0 37.0 33.0 37.0 7 35.52103576050717 37.0 35.0 37.0 33.0 37.0 8 35.48893501284201 37.0 35.0 37.0 33.0 37.0 9 37.10896426806239 39.0 37.0 39.0 33.0 39.0 10-11 36.98731869048605 39.0 37.0 39.0 33.0 39.0 12-13 36.897489593540996 39.0 37.0 39.0 33.0 39.0 14-15 38.086517885967496 40.0 38.0 41.0 33.0 41.0 16-17 38.004949674735656 40.0 38.0 41.0 33.0 41.0 18-19 37.95659570942481 40.0 38.0 41.0 33.0 41.0 20-21 37.85600303408633 40.0 38.0 41.0 32.5 41.0 22-23 37.71758855846616 40.0 37.5 41.0 32.0 41.0 24-25 37.82807629784328 40.0 38.0 41.0 33.0 41.0 26-27 37.77990803461487 40.0 38.0 41.0 33.0 41.0 28-29 37.68116952314563 40.0 37.5 41.0 32.0 41.0 30-31 37.487010139333705 40.0 37.0 41.0 32.0 41.0 32-33 37.26200563962939 40.0 36.5 41.0 31.5 41.0 34-35 37.070184673578595 40.0 36.0 41.0 31.0 41.0 36-37 36.87631955614346 39.0 36.0 41.0 30.5 41.0 38-39 36.71924416395494 39.0 36.0 41.0 30.0 41.0 40-41 36.52399628024445 39.0 35.5 41.0 30.0 41.0 42-43 36.297641869248594 39.0 35.0 41.0 30.0 41.0 44-45 36.02503264071218 39.0 35.0 40.5 29.5 41.0 46-47 35.67878753681901 39.0 35.0 40.0 27.5 41.0 48-49 35.418208946269246 39.0 35.0 40.0 27.0 41.0 50-51 34.707069963973794 38.0 34.0 39.5 25.5 40.5 52-53 34.894719775557604 38.5 34.5 40.0 26.0 40.5 54-55 35.30158018187376 39.0 35.0 40.5 26.5 41.0 56-57 35.19148313110853 39.0 35.0 41.0 26.0 41.0 58-59 34.94140027941021 38.5 35.0 40.0 26.0 41.0 60-61 34.67426854806684 38.0 34.0 40.0 25.5 41.0 62-63 34.33254528988095 38.0 34.0 40.0 24.5 41.0 64-65 33.988502184142185 37.0 34.0 40.0 23.5 41.0 66-67 33.61149838724884 37.0 33.5 39.5 22.5 41.0 68-69 33.21051045217028 36.0 33.0 39.0 22.0 41.0 70-71 32.81683846490088 36.0 33.0 39.0 20.5 40.5 72-73 32.40324564385769 35.0 32.0 38.5 20.0 40.0 74-75 31.967529990886312 35.0 32.0 37.5 18.5 39.5 76-77 31.53659902349274 35.0 31.5 37.0 17.0 39.0 78-79 31.10676584110187 35.0 31.0 36.5 12.5 39.0 80-81 30.693980824117272 35.0 31.0 36.0 9.0 38.5 82-83 30.287131131382793 34.5 30.5 35.5 7.0 37.0 84-85 29.893024886936 34.0 30.0 35.0 4.0 37.0 86-87 29.54072589515546 34.0 30.0 35.0 2.0 36.0 88-89 29.199584027335348 34.0 29.5 35.0 2.0 36.0 90-91 28.808635432528718 34.0 29.0 35.0 2.0 36.0 92-93 28.45066467060736 34.0 29.0 35.0 2.0 35.0 94-95 28.057069821126042 34.0 29.0 35.0 2.0 35.0 96-97 27.55008242315505 34.0 27.5 35.0 2.0 35.0 98-99 26.909290960879712 34.0 26.5 35.0 2.0 35.0 100-101 24.42286792582202 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21898899950424422 1101 2 0.34151165935403327 1101 3 0.21498608500539618 1101 4 0.29999997471222173 1101 5 0.3676573518212862 1101 6 0.11815708841995587 1101 7 0.17378784961812954 1101 8 0.13619270594890764 1101 9 0.27135955691222335 1101 10-11 0.34580747814702306 1101 12-13 0.2965832392648764 1101 14-15 0.3793120956735976 1101 16-17 0.4596797891259641 1101 18-19 0.3972364863917264 1101 20-21 0.37341248276054273 1101 22-23 0.3700324576808782 1101 24-25 0.36754167070559873 1101 26-27 0.24356836439304885 1101 28-29 0.2994608954091902 1101 30-31 0.3276303163183272 1101 32-33 0.30844411427888474 1101 34-35 0.23644581352657923 1101 36-37 0.2579041778814073 1101 38-39 0.33602760837175794 1101 40-41 0.3630857885241383 1101 42-43 0.3458980725977483 1101 44-45 0.30830971683538877 1101 46-47 0.2565585558904644 1101 48-49 0.20797149683049554 1101 50-51 0.35928957636414793 1101 52-53 0.3927734543585544 1101 54-55 0.32688438748147064 1101 56-57 0.0391889461692827 1101 58-59 0.08796860786136307 1101 60-61 0.3941644593628695 1101 62-63 0.34364934658200497 1101 64-65 0.24143439946668366 1101 66-67 0.16953618355285727 1101 68-69 0.10853716568855276 1101 70-71 0.05196962522719417 1101 72-73 0.10865415808812173 1101 74-75 0.08501729213573483 1101 76-77 0.15731955440909218 1101 78-79 0.13773683607711718 1101 80-81 0.3123191333272075 1101 82-83 0.2512612625490185 1101 84-85 0.11878732794149016 1101 86-87 0.061613473740042224 1101 88-89 0.08273679985247995 1101 90-91 0.012310636534277108 1101 92-93 0.06388637038524436 1101 94-95 0.1975469625182278 1101 96-97 -0.0597700654040807 1101 98-99 -0.03850893906447439 1101 100-101 0.030611826475425374 1102 1 0.25015098460310625 1102 2 0.3273366699238025 1102 3 0.29088215253952754 1102 4 0.2915658719676557 1102 5 0.44193314685330165 1102 6 0.16967753885187875 1102 7 0.17383511022718068 1102 8 0.25344308371445123 1102 9 0.4003471708007069 1102 10-11 0.4298560007420633 1102 12-13 0.36976948566125856 1102 14-15 0.35353271075126 1102 16-17 0.4185617819397933 1102 18-19 0.27517829964078544 1102 20-21 0.38403079203141743 1102 22-23 0.3346285135914613 1102 24-25 0.24439457397590303 1102 26-27 0.33190578725926656 1102 28-29 0.2906196489042401 1102 30-31 0.2845880359556219 1102 32-33 0.4924651107624385 1102 34-35 0.31304791624048534 1102 36-37 0.2594388703617412 1102 38-39 0.30013062117172495 1102 40-41 0.47269846867327914 1102 42-43 0.47225857141431504 1102 44-45 0.301889937887303 1102 46-47 0.4593123166214923 1102 48-49 0.4037444185452159 1102 50-51 0.5454570986181508 1102 52-53 0.6010084877539228 1102 54-55 0.7796138792511016 1102 56-57 0.6746611626790724 1102 58-59 0.6445092206909564 1102 60-61 0.5893427915231939 1102 62-63 0.8159297220611563 1102 64-65 0.7228316018940859 1102 66-67 0.6490234825835017 1102 68-69 0.7128885904681894 1102 70-71 0.4924397788752657 1102 72-73 0.3789203375021657 1102 74-75 0.5638269762177224 1102 76-77 0.592420200346659 1102 78-79 0.5316968025093161 1102 80-81 0.5664825375216545 1102 82-83 0.48612541384427743 1102 84-85 0.5413923327122561 1102 86-87 0.41894199756130845 1102 88-89 0.5456362637638961 1102 90-91 0.3084060161072202 1102 92-93 0.31363250427582656 1102 94-95 0.429260619429197 1102 96-97 0.3831451861053843 1102 98-99 0.24653224791765282 1102 100-101 0.23841161520773113 1103 1 0.031922017931108826 1103 2 0.2899652364553873 1103 3 0.15878555192020016 1103 4 0.2071013069720351 1103 5 0.09018039011988321 1103 6 0.1906651341695138 1103 7 0.12220743857304228 1103 8 -1.541423302739986E-4 1103 9 0.02636997616733794 1103 10-11 0.09978465775020595 1103 12-13 0.260937226565396 1103 14-15 0.3001367085297204 1103 16-17 0.3006802188871873 1103 18-19 0.29181016551794414 1103 20-21 0.24384815792752335 1103 22-23 0.16437849175513009 1103 24-25 0.12951323999873665 1103 26-27 0.3427831626809592 1103 28-29 0.26203012737526876 1103 30-31 0.17749032291958144 1103 32-33 0.29693232907792577 1103 34-35 0.09346221318488546 1103 36-37 0.2568630076619769 1103 38-39 0.5184601173540742 1103 40-41 0.4705049477178207 1103 42-43 0.16545198195210986 1103 44-45 0.18542897217775334 1103 46-47 0.463558452415441 1103 48-49 0.24387242062832115 1103 50-51 0.37135687893645297 1103 52-53 0.3646697890013044 1103 54-55 0.46372255329981726 1103 56-57 0.5133092385277678 1103 58-59 0.3849951737519106 1103 60-61 0.32734187522143543 1103 62-63 0.22399218879165517 1103 64-65 0.13773996347731554 1103 66-67 0.009779810586572069 1103 68-69 0.09510014081452312 1103 70-71 0.17189566702052872 1103 72-73 0.035997697432421205 1103 74-75 0.19083900844649193 1103 76-77 0.13463888664020374 1103 78-79 0.1724183407097044 1103 80-81 0.29633790686872175 1103 82-83 0.3333634067478819 1103 84-85 0.26355266244905096 1103 86-87 0.20991395312396932 1103 88-89 0.1947326517880299 1103 90-91 0.16596311604508784 1103 92-93 -0.011114894729097102 1103 94-95 0.03561836934803253 1103 96-97 0.05701056225879597 1103 98-99 0.0025755547122940925 1103 100-101 0.0641264047467267 1104 1 0.11664415950244589 1104 2 0.16075246850212466 1104 3 0.2190920649878798 1104 4 0.2816927047734623 1104 5 0.2394614557240402 1104 6 0.14002593247926853 1104 7 0.14142815746276938 1104 8 0.33435470867006956 1104 9 0.30062591591889287 1104 10-11 0.3442240273928121 1104 12-13 0.30798287298099325 1104 14-15 0.36949634546673593 1104 16-17 0.14172123321227303 1104 18-19 0.34667944408212037 1104 20-21 0.41181559928936906 1104 22-23 0.36484761934086407 1104 24-25 0.3144802748801183 1104 26-27 0.17355552022295484 1104 28-29 0.16309847862798676 1104 30-31 0.11024014089673528 1104 32-33 0.2858339608066345 1104 34-35 0.1098208182868845 1104 36-37 0.09392073682091961 1104 38-39 -0.010468114277252027 1104 40-41 -0.07416602645056258 1104 42-43 -0.1351442266125673 1104 44-45 -0.2527580803028897 1104 46-47 -0.2588812790394428 1104 48-49 0.014571098003443694 1104 50-51 -0.08625469101615835 1104 52-53 0.014078158925329376 1104 54-55 0.18013152177748282 1104 56-57 0.1636813244872144 1104 58-59 0.2369285243569479 1104 60-61 0.3147599649419419 1104 62-63 0.42524747638774585 1104 64-65 0.06495818541564802 1104 66-67 0.078224866488803 1104 68-69 0.15946810836755532 1104 70-71 0.09738467194743805 1104 72-73 -0.0032125914367071573 1104 74-75 0.23396725526500362 1104 76-77 0.11310323919723331 1104 78-79 0.029666646668886187 1104 80-81 0.08365482315608119 1104 82-83 0.17340776225301013 1104 84-85 0.250935575975916 1104 86-87 0.12089529075735683 1104 88-89 0.3676454192946075 1104 90-91 0.09569950676661776 1104 92-93 -0.18076520986312516 1104 94-95 -0.019229133477228544 1104 96-97 0.012519935876873944 1104 98-99 -0.1475134466411525 1104 100-101 -0.4411193149246948 1105 1 0.14498304222534486 1105 2 0.12870687813244786 1105 3 0.21110663569278998 1105 4 0.22287486704330917 1105 5 0.23920533005171052 1105 6 0.18846356587805246 1105 7 0.11449512009687624 1105 8 0.3850135208161163 1105 9 0.22844116393391545 1105 10-11 0.21393218641840406 1105 12-13 0.1421742273166373 1105 14-15 0.15922143724510107 1105 16-17 0.3042265691637809 1105 18-19 0.2925216706679734 1105 20-21 0.1214537506311757 1105 22-23 0.13839054884938662 1105 24-25 0.07045582910761539 1105 26-27 -0.23924791755273134 1105 28-29 -0.16293029282253713 1105 30-31 -0.45944027118100905 1105 32-33 -0.13400510689591982 1105 34-35 -0.4155085877861424 1105 36-37 -0.12370284381598395 1105 38-39 -0.21328691959328694 1105 40-41 -0.3803763625443679 1105 42-43 -0.3377901470997742 1105 44-45 -0.5462311444196857 1105 46-47 -0.12207175116444091 1105 48-49 -0.19650704878502268 1105 50-51 -0.33092229681028584 1105 52-53 -0.3242553167000395 1105 54-55 -0.19593709144429283 1105 56-57 -0.08801195079096402 1105 58-59 -0.0335921222180815 1105 60-61 -0.4008594323089767 1105 62-63 -0.2672046423407295 1105 64-65 -0.3844961246102514 1105 66-67 -0.3706922910853976 1105 68-69 -0.19785993170188476 1105 70-71 -0.06205355907792409 1105 72-73 -0.4347964441359906 1105 74-75 -0.41822119631301646 1105 76-77 -0.186673362299679 1105 78-79 -0.38231292019414553 1105 80-81 -0.368452521959032 1105 82-83 -0.38974002225992166 1105 84-85 -0.2482455333328275 1105 86-87 -0.5385279996037795 1105 88-89 -0.5341702271381301 1105 90-91 -0.6391428336528584 1105 92-93 -0.7248992205065683 1105 94-95 -0.6318212675732475 1105 96-97 -1.0704320547887676 1105 98-99 -1.1924997866052927 1105 100-101 -0.5733648256108275 1106 1 0.02942015815405341 1106 2 0.25726286018359446 1106 3 0.22910775299109432 1106 4 0.28505071537907156 1106 5 0.38685089419725216 1106 6 0.19082070487356617 1106 7 0.303824921325905 1106 8 0.21238372499337288 1106 9 0.28926183755666557 1106 10-11 0.2726993738902408 1106 12-13 0.17468670681781617 1106 14-15 0.4307411431433579 1106 16-17 0.19750926365478705 1106 18-19 0.18094411755124895 1106 20-21 0.41514281732668223 1106 22-23 0.1587659250365192 1106 24-25 0.28822717941667264 1106 26-27 0.16795775543928215 1106 28-29 0.21139257034446501 1106 30-31 0.21949865585220607 1106 32-33 0.2459511022190668 1106 34-35 0.2496028717298202 1106 36-37 0.25032267383553375 1106 38-39 0.1704852236371508 1106 40-41 -0.07851750254905454 1106 42-43 0.13407195666479765 1106 44-45 0.2136650549640038 1106 46-47 0.3302421375742739 1106 48-49 0.27143459941017056 1106 50-51 0.10668019728785083 1106 52-53 0.30879869205813293 1106 54-55 0.03026085056431782 1106 56-57 0.25421471659653605 1106 58-59 0.15051971573762302 1106 60-61 0.2991432708320758 1106 62-63 0.32163048407616657 1106 64-65 0.10433539761858412 1106 66-67 0.13462388631273114 1106 68-69 0.2826837600679468 1106 70-71 0.3294018120633382 1106 72-73 0.09801878716277201 1106 74-75 0.2850505054043815 1106 76-77 0.3488576247072217 1106 78-79 0.3858783180246661 1106 80-81 0.39689951711036286 1106 82-83 0.5698669410314245 1106 84-85 0.5551126688981647 1106 86-87 0.6676432714131444 1106 88-89 0.4378946122051879 1106 90-91 0.4053058156776608 1106 92-93 0.04441111810690046 1106 94-95 -0.122139517379523 1106 96-97 0.029685982370409647 1106 98-99 0.04104889696501246 1106 100-101 0.11775445499323567 1107 1 -0.053339184058526 1107 2 0.22995255481889387 1107 3 0.14355140506919994 1107 4 0.05834806484251942 1107 5 0.037844365003770974 1107 6 0.1352734525580317 1107 7 -0.0227692651724567 1107 8 0.09915837173795694 1107 9 0.027389509124141398 1107 10-11 -0.03187853446191724 1107 12-13 -0.08224815924617701 1107 14-15 0.05975570038376077 1107 16-17 0.015519204768203565 1107 18-19 -0.11036616219202244 1107 20-21 0.07929289056125555 1107 22-23 0.3065812714168459 1107 24-25 0.26150082906075767 1107 26-27 0.008204959501135534 1107 28-29 0.22610887710126093 1107 30-31 0.3016644350211237 1107 32-33 0.13109345059360322 1107 34-35 0.022500953966051895 1107 36-37 0.14971053525777478 1107 38-39 0.031950171390789706 1107 40-41 0.154653490580138 1107 42-43 0.20912565490418444 1107 44-45 0.08638421423959386 1107 46-47 0.15031246838960044 1107 48-49 -0.030555544881153196 1107 50-51 0.09472139654141642 1107 52-53 0.3446814996540368 1107 54-55 0.39814289805789826 1107 56-57 0.4675045504415891 1107 58-59 0.2951409243003056 1107 60-61 0.4113555101874411 1107 62-63 0.3543842749797079 1107 64-65 0.3756993969524274 1107 66-67 0.09887345608628095 1107 68-69 0.2929910463745813 1107 70-71 0.05497818740015248 1107 72-73 0.14879511638102372 1107 74-75 0.14695422008417225 1107 76-77 0.11913698365913916 1107 78-79 0.2838644739139653 1107 80-81 0.21480910763726868 1107 82-83 0.23990033429042512 1107 84-85 0.04879164950701309 1107 86-87 0.1142065840927593 1107 88-89 -0.026424348931382013 1107 90-91 0.08134850097394875 1107 92-93 0.07561161003828332 1107 94-95 -0.03273888794648272 1107 96-97 -0.15234677889340986 1107 98-99 -0.009689000113716872 1107 100-101 -0.3922955494905125 1108 1 -0.2431582373505421 1108 2 0.09783155902097462 1108 3 -0.07994454929894346 1108 4 0.1800762716228661 1108 5 0.19469111308525555 1108 6 0.013816422475962042 1108 7 -0.1417115369842179 1108 8 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-2.2041689537385345 2316 76-77 -2.2692744732577665 2316 78-79 -2.2431493522742088 2316 80-81 -2.635670076933099 2316 82-83 -2.2295003858462046 2316 84-85 -2.1033160011288174 2316 86-87 -2.1881354916847577 2316 88-89 -2.1942629600032113 2316 90-91 -1.9957420165018718 2316 92-93 -2.4332497690995076 2316 94-95 -2.2376928187829073 2316 96-97 -2.209425765480592 2316 98-99 -2.387693286123266 2316 100-101 -2.601340770154735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1678.0 3 579.0 4 592.0 5 632.0 6 676.0 7 837.0 8 971.0 9 1236.0 10 1500.0 11 1914.0 12 2349.0 13 2833.0 14 3406.0 15 3982.0 16 5039.0 17 6779.0 18 6341.0 19 4073.0 20 4053.0 21 4239.0 22 4503.0 23 4851.0 24 5442.0 25 6289.0 26 7275.0 27 8663.0 28 10140.0 29 12445.0 30 15121.0 31 18850.0 32 23965.0 33 31125.0 34 43494.0 35 63166.0 36 96958.0 37 141893.0 38 128072.0 39 24067.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60119961879669 18.615380438898168 15.18483645263749 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30.427477546017002 21.397808214089522 20-21 22.321243612144105 25.681930850433094 30.61070545358956 21.386120083833244 22-23 22.036950395897097 25.497701761090298 30.62954245938349 21.83580538362911 24-25 21.82987732059494 25.699821152055037 30.703635241616144 21.766666285733876 26-27 21.670575933581507 25.91572553803607 30.557563360122046 21.856135168260373 28-29 21.604039275655992 26.02737182092392 30.689981022147016 21.678607881273066 30-31 21.809923919285303 25.761021418592495 30.539850238412903 21.889204423709298 32-33 21.586561949140197 25.83245005299682 30.533596555635235 22.047391442227752 34-35 21.96562797301892 25.937581244661068 30.3464343771695 21.75035640515052 36-37 22.019695848558523 25.66974170268811 30.487210840430485 21.823351608322884 38-39 21.72223091213357 26.096803707758998 30.322029068110574 21.858936311996864 40-41 21.968556352011152 25.917154015593262 30.3807892624199 21.733500369975687 42-43 21.83238220400117 25.962188145047822 30.096279524887688 22.109150126063323 44-45 21.880506338516568 26.12144496322025 30.097615083590995 21.90043361467219 46-47 22.072906637563815 26.38383763352694 29.57198526953943 21.971270459369812 48-49 22.08165189380683 26.41701604896828 29.52038797505875 21.980944082166147 50-51 22.086334326601396 26.608965696539943 29.27043365721681 22.03426631964185 52-53 22.12425218986235 26.734319557056413 29.03210369633909 22.109324556742145 54-55 22.060641730098517 26.583593977110965 29.150128673148775 22.205635619641743 56-57 21.86345829003239 26.946578243463794 29.008310209737264 22.181653256766552 58-59 22.037312153591223 26.574410033712358 29.1833323810068 22.20494543168962 60-61 22.078743936854 26.42663834401028 29.381589463857544 22.113028255278177 62-63 21.759371687660884 26.53335409422971 29.55327884074202 22.153995377367387 64-65 21.88805060285884 26.59599922575798 29.284133252480753 22.23181691890243 66-67 21.794774722778588 26.66047126926121 29.124463200482296 22.420290807477908 68-69 21.83835546767852 26.652932705495108 29.27583832403767 22.2328735027887 70-71 21.944704793146606 26.524325776236008 29.104278227673035 22.426691202944355 72-73 21.872754313503904 26.602786187072557 28.984588965259096 22.539870534164436 74-75 21.84817883029966 26.813186970184233 28.881053391785255 22.457580807730853 76-77 21.95363630453528 26.763964368151942 28.783311984420173 22.499087342892597 78-79 21.901049312471383 26.79114974329741 28.6972566718743 22.610544272356915 80-81 21.84866984486287 26.704136228007 28.726449337494714 22.720744589635423 82-83 21.94979409520489 26.8536432717286 28.796450035579664 22.400112597486846 84-85 21.945422893639577 26.783799595825673 28.579868004008883 22.690909506525866 86-87 21.830669500151796 26.68907262889083 28.705466364269515 22.77479150668786 88-89 21.963704314309265 26.480307097559475 28.65796252141154 22.898026066719716 90-91 21.792062819639995 26.4160958182391 29.0077917327765 22.78404962934441 92-93 21.81511495570878 26.459638511609167 28.907068806248137 22.81817772643392 94-95 21.72306769194504 26.502898328148603 28.845276064416815 22.928757915489538 96-97 21.58793068099585 26.58369093193756 28.981310100318108 22.84706828674848 98-99 21.583519678795195 26.84810642052396 28.673601337400783 22.89477256328006 100-101 21.681576847148797 26.885981238965623 28.350325056462438 23.08211685742314 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 168.0 1 131.5 2 125.0 3 141.5 4 120.0 5 136.0 6 251.5 7 582.0 8 1064.5 9 1278.5 10 1117.5 11 931.5 12 889.0 13 960.0 14 1039.0 15 1050.5 16 1071.0 17 1135.0 18 1226.5 19 1322.5 20 1483.5 21 1755.0 22 2053.5 23 2481.5 24 3021.0 25 3740.0 26 4925.5 27 6223.0 28 6976.0 29 7741.5 30 8861.5 31 10367.0 32 11979.0 33 13211.0 34 14315.5 35 15464.0 36 17005.5 37 18569.0 38 19644.5 39 20202.5 40 20703.0 41 21261.0 42 21657.5 43 22214.0 44 23016.0 45 23793.0 46 24426.0 47 25136.0 48 25840.5 49 25924.0 50 25203.5 51 24514.0 52 24275.5 53 24168.5 54 23650.0 55 21886.5 56 19773.5 57 17748.5 58 16206.5 59 14509.5 60 11966.0 61 9846.0 62 7674.5 63 5513.5 64 3918.5 65 2832.5 66 2061.5 67 1534.0 68 1186.0 69 938.5 70 696.0 71 451.0 72 303.5 73 187.0 74 110.5 75 71.5 76 50.0 77 32.5 78 21.0 79 15.0 80 12.0 81 7.5 82 5.0 83 6.0 84 4.5 85 4.5 86 5.0 87 2.5 88 1.5 89 1.0 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022427097647868852 2 0.008713713098853505 3 0.0115706682132317 4 0.021712858869274303 5 0.0504252577687752 6 0.04214008793707842 7 0.01742742619770701 8 0.04285432671567297 9 0.028283855632344163 10-11 0.02371272744933904 12-13 0.033854918105381646 14-15 0.0187130559991772 16-17 0.017070306808409732 18-19 0.016356068029815183 20-21 0.012142059236107342 22-23 0.007642354930961679 24-25 0.001428477557189099 26-27 0.0 28-29 0.002785531236518743 30-31 0.0 32-33 5.713910228756396E-4 34-35 0.0 36-37 0.0 38-39 7.142387785945495E-5 40-41 0.0 42-43 1.428477557189099E-4 44-45 7.142387785945495E-5 46-47 0.0 48-49 1.428477557189099E-4 50-51 0.0 52-53 2.856955114378198E-4 54-55 0.0023569879693620135 56-57 4.999671450161846E-4 58-59 8.570865343134594E-4 60-61 0.0024998357250809234 62-63 0.0017141730686269189 64-65 9.285104121729144E-4 66-67 0.009142256366010234 68-69 0.011142124946074972 70-71 0.018570208243458286 72-73 0.01328484128185862 74-75 0.013927656182593715 76-77 0.023784151327198497 78-79 0.015927524762658452 80-81 0.03971167608985695 82-83 0.02971233318953326 84-85 0.014356199449750445 86-87 0.011927787602528977 88-89 0.010427886167480423 90-91 0.019641566411350113 92-93 0.027069649708733424 94-95 0.010499310045339877 96-97 0.017498850075566463 98-99 0.025855443785122695 100-101 0.03492627627327347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 700046.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.9667823965272 #Duplication Level Percentage of deduplicated Percentage of total 1 85.47046402021343 61.51034285472906 2 9.146245437223863 13.164517102518388 3 2.354832378697579 5.084091281340754 4 1.0118443208228953 2.912767202232926 5 0.5491862357082987 1.9761583160193514 6 0.33559268572130757 1.4490915472302888 7 0.21814306963049193 1.0989338376385664 8 0.1696459167482012 0.976709662006173 9 0.1125728725130614 0.7291356677909538 >10 0.5962153987330702 7.539361271363052 >50 0.02489965153968605 1.2304171391318763 >100 0.009561242260820542 1.0620984269616842 >500 1.9919254682502143E-4 0.10694094017585312 >1k 5.975776404750643E-4 1.1594347508609921 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3630 0.518537353259643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2769 0.39554543558566146 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1689 0.2412698594092388 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 0.10656442576630679 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 1.428477557189099E-4 0.0 0.0 0.0 0.0 18-19 2.1427163357836486E-4 0.0 0.0 0.0 0.0 20-21 2.856955114378198E-4 0.0 0.0 0.0 0.0 22-23 2.856955114378198E-4 0.0 0.0 2.856955114378198E-4 0.0 24-25 4.285432671567297E-4 0.0 0.0 7.856626564540045E-4 0.0 26-27 4.285432671567297E-4 0.0 0.0 0.0017141730686269186 0.0 28-29 4.285432671567297E-4 0.0 0.0 0.0034997700151132927 0.0 30-31 4.285432671567297E-4 0.0 0.0 0.005785334106615851 0.0 32-33 4.285432671567297E-4 0.0 0.0 0.009213680243869688 0.0 34-35 4.285432671567297E-4 0.0 0.0 0.013641960671155896 0.0 36-37 4.285432671567297E-4 0.0 0.0 0.017855969464863737 0.0 38-39 4.285432671567297E-4 0.0 0.0 0.023212760304322858 0.0 40-41 4.285432671567297E-4 0.0 0.0 0.0274267690980307 0.0 42-43 4.285432671567297E-4 0.0 0.0 0.04014021935701368 0.0 44-45 4.285432671567297E-4 0.0 0.0 0.054924962073920856 0.0 46-47 4.285432671567297E-4 0.0 0.0 0.06663847804287146 0.0 48-49 4.285432671567297E-4 0.0 0.0 0.08070898198118409 0.0 50-51 4.285432671567297E-4 0.0 0.0 0.09635081123240473 0.0 52-53 4.285432671567297E-4 0.0 0.0 0.13277698894072676 7.142387785945495E-5 54-55 4.285432671567297E-4 0.0 0.0 0.16334640866457345 1.428477557189099E-4 56-57 4.285432671567297E-4 0.0 0.0 0.2265565405701911 1.428477557189099E-4 58-59 4.285432671567297E-4 0.0 0.0 0.3158363878945098 1.428477557189099E-4 60-61 4.285432671567297E-4 0.0 0.0 0.39040291637978075 1.428477557189099E-4 62-63 4.285432671567297E-4 0.0 0.0 0.5027526762527034 1.428477557189099E-4 64-65 4.285432671567297E-4 0.0 0.0 0.5761049988143636 1.428477557189099E-4 66-67 4.285432671567297E-4 0.0 0.0 0.7070249669307446 1.428477557189099E-4 68-69 4.285432671567297E-4 0.0 0.0 0.8655859757787345 1.428477557189099E-4 70-71 4.285432671567297E-4 0.0 0.0 0.9707219239878522 1.428477557189099E-4 72-73 4.285432671567297E-4 0.0 0.0 1.182922265108293 1.428477557189099E-4 74-75 5.713910228756396E-4 0.0 0.0 1.3811235261682804 1.428477557189099E-4 76-77 5.713910228756396E-4 0.0 0.0 1.5039011722086835 1.428477557189099E-4 78-79 5.713910228756396E-4 0.0 0.0 1.6298928927527618 1.428477557189099E-4 80-81 5.713910228756396E-4 0.0 0.0 1.8688771880704982 1.428477557189099E-4 82-83 5.713910228756396E-4 0.0 0.0 2.0657928193290154 1.428477557189099E-4 84-85 5.713910228756396E-4 0.0 0.0 2.3704870822774504 1.428477557189099E-4 86-87 5.713910228756396E-4 0.0 0.0 2.8404561985926637 1.428477557189099E-4 88-89 5.713910228756396E-4 0.0 0.0 3.4955560063195845 1.428477557189099E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1900 0.0 45.264225 1 GTATCAA 3320 0.0 40.072834 1 AATACCG 75 6.9001053E-6 38.014668 5 ATACCGT 80 1.0741611E-5 35.63875 6 TATCAAC 4080 0.0 32.72709 2 ATCAACG 4085 0.0 32.33806 3 TCAACGC 4175 0.0 31.9824 4 CAACGCA 4200 0.0 31.792028 5 AACGCAG 4355 0.0 30.551394 6 ACGCAGA 4755 0.0 27.27987 7 CGCAGAG 4780 0.0 27.037788 8 TACCGTG 110 9.451209E-5 25.91724 7 GCAGAGT 5030 0.0 24.843792 9 GTACATG 2500 0.0 23.947529 1 GAGTACT 3130 0.0 23.37545 12-13 TACATGG 2470 0.0 23.08583 2 GAGTACG 105 0.002191361 22.626162 1 AGAGTAC 4410 0.0 21.38558 10-11 AGTACTT 3295 0.0 21.339777 12-13 GTACTTT 3445 0.0 21.306267 14-15 >>END_MODULE