FastQCFastQC Report
Thu 26 May 2016
SRR937021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700046
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31460.4493990394916905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25540.3648331681060959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.2262708450587533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.1025646886061773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.041.096481
GTATCAA31850.035.0655251
CACGACT953.4883058E-530.0007677
TAAGGTG3200.029.6882615
ATCAACG40350.027.0724123
TATCAAC41050.026.8459992
TCAACGC40800.026.6574134
CAACGCA41750.026.0545585
ACGACTC1301.0011785E-525.5775768
AACGCAG43050.025.2677766
GGACCGT950.001225892925.0006396
GTAAGGT3550.022.7438094
ACGCAGA48200.022.4694547
CGCAGAG48700.021.9461468
GGTAAGG3700.021.8217663
GTACATG24900.021.7584251
GAGTACT29550.021.617612-13
GACGCAT550.002877249421.59146172-73
GGACCAT3301.8189894E-1221.5914616
TACATGG24550.021.0901532
Produced by FastQC (version 0.11.5)