##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937021_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700046 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26773669158884 33.0 31.0 34.0 31.0 34.0 2 32.39621110612731 34.0 31.0 34.0 31.0 34.0 3 32.4990457769918 34.0 31.0 34.0 31.0 34.0 4 36.12351474045991 37.0 35.0 37.0 35.0 37.0 5 36.04082160315179 37.0 35.0 37.0 35.0 37.0 6 36.08074326544256 37.0 35.0 37.0 35.0 37.0 7 36.030549421038046 37.0 35.0 37.0 35.0 37.0 8 36.00518965896527 37.0 35.0 37.0 35.0 37.0 9 37.70592361073415 39.0 37.0 39.0 35.0 39.0 10-11 37.61886647448882 39.0 37.0 39.0 35.0 39.0 12-13 37.52892667053308 39.0 37.0 39.0 35.0 39.0 14-15 38.77194784342743 40.0 38.0 41.0 35.0 41.0 16-17 38.68482856840836 40.0 38.0 41.0 35.0 41.0 18-19 38.81779983029686 40.0 38.0 41.0 35.0 41.0 20-21 38.83766995311737 40.0 38.0 41.0 35.0 41.0 22-23 38.7548482528291 40.0 38.0 41.0 34.5 41.0 24-25 38.64666393351294 40.0 38.0 41.0 34.0 41.0 26-27 38.46589724103845 40.0 38.0 41.0 34.0 41.0 28-29 38.39139927947592 40.0 38.0 41.0 34.0 41.0 30-31 38.40063795807704 40.0 38.0 41.0 34.0 41.0 32-33 38.241606980112735 40.0 38.0 41.0 34.0 41.0 34-35 38.09261248546524 40.0 38.0 41.0 33.5 41.0 36-37 37.951481045531295 40.0 37.0 41.0 33.0 41.0 38-39 37.79116443776552 40.0 37.0 41.0 33.0 41.0 40-41 37.61783439945375 40.0 37.0 41.0 33.0 41.0 42-43 37.38459329815469 40.0 37.0 41.0 32.5 41.0 44-45 37.11984498161549 40.0 36.5 41.0 31.5 41.0 46-47 36.779633766923894 39.5 36.0 41.0 31.0 41.0 48-49 36.42417141159295 39.0 36.0 41.0 30.0 41.0 50-51 36.12808515440414 39.0 35.0 40.5 30.0 41.0 52-53 35.87229267790974 39.0 35.0 40.0 29.0 41.0 54-55 35.57558074755087 38.5 35.0 40.0 28.0 41.0 56-57 35.281947900566536 38.0 34.5 40.0 28.0 41.0 58-59 34.92850898369535 38.0 34.0 40.0 27.0 41.0 60-61 34.59510375032498 37.5 34.0 40.0 26.0 41.0 62-63 34.208017044594214 37.0 33.5 40.0 26.0 41.0 64-65 33.81985969493433 36.5 33.0 39.0 26.0 40.5 66-67 33.42254580413287 36.0 33.0 39.0 25.0 40.0 68-69 33.108314310773864 35.5 32.5 39.0 24.0 40.0 70-71 32.64850738380049 35.0 32.0 38.0 22.5 40.0 72-73 32.13200918225374 35.0 31.5 37.5 21.5 39.5 74-75 31.638505898183833 35.0 31.0 37.0 20.0 39.0 76-77 30.2280143019173 33.5 29.5 35.5 19.5 37.5 78-79 30.969925547749718 34.0 31.0 36.0 20.0 38.0 80-81 30.73654374141128 34.0 31.0 35.5 18.0 37.0 82-83 30.41733186104913 34.0 30.0 35.0 17.0 37.0 84-85 30.082717421426594 34.0 30.0 35.0 13.5 36.5 86-87 29.758757281664344 34.0 30.0 35.0 9.0 36.0 88-89 29.38700385403245 34.0 29.5 35.0 6.5 36.0 90-91 28.95975621602009 34.0 29.0 35.0 2.0 35.0 92-93 28.483924627810172 33.5 29.0 35.0 2.0 35.0 94-95 27.896417521134325 33.0 28.0 35.0 2.0 35.0 96-97 27.237998645803273 33.0 26.5 34.5 2.0 35.0 98-99 26.443566565625687 33.0 25.5 34.0 2.0 35.0 100-101 22.95442656625422 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10154949772430655 1101 2 0.05281832482211257 1101 3 0.1248414690922317 1101 4 0.11875387170378104 1101 5 0.19851412067945517 1101 6 0.17224630622124693 1101 7 0.12977211347911322 1101 8 0.0010412357703941666 1101 9 0.18846602282425096 1101 10-11 0.11032588347497807 1101 12-13 0.08885318852023971 1101 14-15 0.20636351984379786 1101 16-17 0.20186128336547426 1101 18-19 0.06469703286438744 1101 20-21 0.1822919865714283 1101 22-23 0.1952372514684484 1101 24-25 0.17986725232696443 1101 26-27 0.09422045100944132 1101 28-29 0.19624577389618025 1101 30-31 0.10968127706049557 1101 32-33 0.1485139096726229 1101 34-35 0.1731634078406188 1101 36-37 0.036283879795213636 1101 38-39 -0.058629579976177126 1101 40-41 -0.07101515970558836 1101 42-43 0.0830325295235852 1101 44-45 -0.027057549847114615 1101 46-47 -0.04378865908856966 1101 48-49 -0.12223918145411261 1101 50-51 -0.22733194506532328 1101 52-53 -0.3741347033277833 1101 54-55 -0.3792019028357885 1101 56-57 -0.17874549668227502 1101 58-59 -0.37037984568451776 1101 60-61 -0.06001034014139606 1101 62-63 -0.32134538171941784 1101 64-65 -0.2936137348549721 1101 66-67 -0.28574706086896384 1101 68-69 -0.4950336543195135 1101 70-71 -0.7248415847047944 1101 72-73 -0.6064008749889922 1101 74-75 -0.48854846689761544 1101 76-77 -0.5273559707232671 1101 78-79 -0.5742538329288642 1101 80-81 -0.38121656084071986 1101 82-83 -0.3579639781062234 1101 84-85 -0.5292033516102528 1101 86-87 -0.8330739588567546 1101 88-89 -0.5639267927074023 1101 90-91 -0.549530090549684 1101 92-93 -0.6176434433525415 1101 94-95 -0.7940489815153491 1101 96-97 -0.8175820158859892 1101 98-99 -0.3224115035156814 1101 100-101 0.05212743944534637 1102 1 0.21133908582893923 1102 2 0.23328615375914552 1102 3 0.26775946682627705 1102 4 0.13038572944168436 1102 5 0.14740177199323767 1102 6 0.10527546857165504 1102 7 0.0807417862185531 1102 8 0.11845702566760963 1102 9 0.15652870827424437 1102 10-11 0.09187157362675435 1102 12-13 0.16655729367892746 1102 14-15 0.2880573145258225 1102 16-17 0.3187814755245526 1102 18-19 0.17469578109690787 1102 20-21 0.21954132971283258 1102 22-23 0.1170806579109609 1102 24-25 0.17561028489974007 1102 26-27 0.2068740958881179 1102 28-29 0.2652178428762326 1102 30-31 0.32896762909073374 1102 32-33 0.21360965072536686 1102 34-35 0.277344949890427 1102 36-37 0.2136245253240432 1102 38-39 0.2981576821400935 1102 40-41 0.3219299809458178 1102 42-43 0.32937238694982085 1102 44-45 0.260818954349503 1102 46-47 0.21345690320411137 1102 48-49 0.27795726940188104 1102 50-51 0.37737840488032504 1102 52-53 0.3446611602193457 1102 54-55 0.31456659426407185 1102 56-57 0.3556883276078864 1102 58-59 0.2992764802757364 1102 60-61 0.40809417733417064 1102 62-63 0.4504152547915794 1102 64-65 0.46059150136504456 1102 66-67 0.2740797609317127 1102 68-69 0.10551903934360496 1102 70-71 0.3026583418496642 1102 72-73 0.41888227353832264 1102 74-75 0.307958846362304 1102 76-77 0.2868577851795209 1102 78-79 0.30640418802674674 1102 80-81 0.30230787991938257 1102 82-83 0.327868568614722 1102 84-85 0.3770312807085965 1102 86-87 0.21348700158100442 1102 88-89 0.27272968525318575 1102 90-91 0.48544691141610485 1102 92-93 0.3297532634771265 1102 94-95 0.28845079814803043 1102 96-97 0.420200797346471 1102 98-99 0.2604411340733641 1102 100-101 0.7959659972048385 1103 1 0.195848477098032 1103 2 0.1695207797481686 1103 3 0.05890446498872848 1103 4 0.0353960543168057 1103 5 0.12191918914291477 1103 6 0.12862138732074158 1103 7 0.002589980065820896 1103 8 0.09877301793508053 1103 9 0.10311045771844363 1103 10-11 0.21599956252565988 1103 12-13 0.17998787145992878 1103 14-15 0.19492990737965954 1103 16-17 0.1967641943369216 1103 18-19 0.2567496978749517 1103 20-21 0.15167773013011043 1103 22-23 0.30798990613151034 1103 24-25 0.2625324668186053 1103 26-27 0.19894586077430887 1103 28-29 0.18783937234208992 1103 30-31 0.3613253592332697 1103 32-33 0.4481326381987287 1103 34-35 0.3137077922057756 1103 36-37 0.19970077613363202 1103 38-39 0.15712755130341094 1103 40-41 0.27935655732270703 1103 42-43 0.4144702054594518 1103 44-45 0.35024901794677277 1103 46-47 0.33398807806447905 1103 48-49 0.3554589297868205 1103 50-51 0.40583814445945166 1103 52-53 0.37595838642887003 1103 54-55 0.4570061982766802 1103 56-57 0.34649976555893147 1103 58-59 0.40990302632896913 1103 60-61 0.28188473316347995 1103 62-63 0.5436967522527922 1103 64-65 0.5065994085878458 1103 66-67 0.15867023501899524 1103 68-69 0.14552319236599942 1103 70-71 0.12673746681443987 1103 72-73 0.3115060443178592 1103 74-75 0.42725194262178334 1103 76-77 0.21179973350735182 1103 78-79 0.3348029506897561 1103 80-81 0.4338120156375176 1103 82-83 0.5827421898360079 1103 84-85 0.38347958958798856 1103 86-87 0.26114911365657534 1103 88-89 0.25583331140206766 1103 90-91 0.27952992571930935 1103 92-93 0.23711161162895067 1103 94-95 0.3322577683039505 1103 96-97 0.3571687943536581 1103 98-99 0.2795808816289309 1103 100-101 0.38272296464151623 1104 1 0.04131778335649017 1104 2 0.08056744459832998 1104 3 0.04928070508914573 1104 4 0.0032394298020150813 1104 5 -0.0431665961014005 1104 6 -0.042548204367406584 1104 7 0.07120477506119727 1104 8 -0.013603195364424892 1104 9 0.1450918489536548 1104 10-11 0.1832399175483701 1104 12-13 -0.0911318035343811 1104 14-15 -0.13652475664860475 1104 16-17 0.0031064062159842365 1104 18-19 -0.01090484286913096 1104 20-21 0.10315580028596116 1104 22-23 0.002367410341484799 1104 24-25 0.03583641593261433 1104 26-27 0.0028424896665839583 1104 28-29 -0.2082088335603629 1104 30-31 -0.044354423500699625 1104 32-33 -0.09206760926300461 1104 34-35 0.010785488042245106 1104 36-37 0.03739000172135576 1104 38-39 -0.08298849663447783 1104 40-41 -0.01019879325553319 1104 42-43 -0.2009071513455254 1104 44-45 -0.16750993467368858 1104 46-47 0.019686554432773562 1104 48-49 0.14509914991162987 1104 50-51 0.19932429508950378 1104 52-53 0.1667571875348557 1104 54-55 0.09877519230454368 1104 56-57 -0.05517272286083852 1104 58-59 -0.030717325388302186 1104 60-61 0.05146000575106768 1104 62-63 0.12772151182630154 1104 64-65 0.37238547926183685 1104 66-67 0.14895323028863316 1104 68-69 0.11611645283063865 1104 70-71 0.05159888377851729 1104 72-73 0.3048432265595764 1104 74-75 0.38272334828481647 1104 76-77 0.17053079978153463 1104 78-79 -0.007854493486181724 1104 80-81 0.18658144294675338 1104 82-83 0.19196427110529868 1104 84-85 0.015817455252896906 1104 86-87 -0.07975342930305018 1104 88-89 -0.13609648808246178 1104 90-91 0.12618014705015312 1104 92-93 -0.06012164115276519 1104 94-95 -0.19549680683463322 1104 96-97 0.076310338890071 1104 98-99 0.15368137610571253 1104 100-101 0.09225638314218543 1105 1 -0.033569094471559424 1105 2 0.13535984051170402 1105 3 -0.03536313793427581 1105 4 -0.06183925769620657 1105 5 -0.08205927944469238 1105 6 -0.06882812126885085 1105 7 0.03755555339865424 1105 8 -0.08407759702639339 1105 9 -0.10837296222480575 1105 10-11 -0.0030943549124273773 1105 12-13 -0.03630596899156302 1105 14-15 -0.244383546027926 1105 16-17 -0.1843306295562357 1105 18-19 -0.201206928870441 1105 20-21 -0.08659205821884086 1105 22-23 -0.17274357744985736 1105 24-25 -0.1741702717932796 1105 26-27 -0.19319467701431137 1105 28-29 -0.036154990252356356 1105 30-31 -0.022613622681092238 1105 32-33 -0.1521361940263759 1105 34-35 -0.14320082346348784 1105 36-37 -0.21535196589297811 1105 38-39 -0.2669009585713624 1105 40-41 -0.33274710140316444 1105 42-43 -0.1226572011477387 1105 44-45 0.0339030252853334 1105 46-47 0.03425939372814213 1105 48-49 -0.12063052367042815 1105 50-51 0.11957031802697315 1105 52-53 0.00583911929159342 1105 54-55 -0.10077658776887688 1105 56-57 -0.27613305184004133 1105 58-59 -0.3572690200864983 1105 60-61 -0.17583658777749633 1105 62-63 0.02321583437390018 1105 64-65 -0.40658019989807315 1105 66-67 -0.4467033051847693 1105 68-69 -0.5556520949367965 1105 70-71 -0.6996347882098704 1105 72-73 -0.3964409591030744 1105 74-75 -0.48900464625117124 1105 76-77 -0.6674554832657904 1105 78-79 -0.3586171788215644 1105 80-81 -0.2600234689744738 1105 82-83 -0.23319651890010817 1105 84-85 -0.3243153032205939 1105 86-87 -0.22048523726570934 1105 88-89 -0.2858816271017872 1105 90-91 -0.5499795330773374 1105 92-93 -0.704021581390105 1105 94-95 -0.8278625542947218 1105 96-97 -0.8172673098762608 1105 98-99 -0.8213634458869485 1105 100-101 0.01392674940189309 1106 1 0.07445382816454327 1106 2 0.14301153177694914 1106 3 -0.020167881045772162 1106 4 0.008049553101940887 1106 5 0.0483780887771772 1106 6 -0.04639565474275287 1106 7 -0.0030798649574705905 1106 8 0.040241661952535424 1106 9 -0.01737622401500971 1106 10-11 -0.08263608239976605 1106 12-13 -0.04481816015769624 1106 14-15 0.14098435146896549 1106 16-17 -0.03895300075156882 1106 18-19 -0.14908406209925573 1106 20-21 0.04103584540517602 1106 22-23 -0.006376058392710604 1106 24-25 0.012701473174381306 1106 26-27 -0.031340502463486075 1106 28-29 0.08286521122175827 1106 30-31 0.03805297190181278 1106 32-33 -0.08691390572736424 1106 34-35 -0.26561346695522303 1106 36-37 -0.15997091310964606 1106 38-39 0.04419453439146537 1106 40-41 0.015545414500309107 1106 42-43 0.011414089692216578 1106 44-45 -0.18151569126329292 1106 46-47 -0.04051939922926806 1106 48-49 -0.09713301957066278 1106 50-51 -0.17942591559649657 1106 52-53 -0.2677097444881227 1106 54-55 -0.492967213275719 1106 56-57 -0.1463948961030681 1106 58-59 -0.34900551789695555 1106 60-61 -0.6107827952705946 1106 62-63 -0.3693045743410224 1106 64-65 -0.36628447626721083 1106 66-67 0.04572519673445896 1106 68-69 -0.2705004487238938 1106 70-71 -0.3847026370502178 1106 72-73 -0.4365429576253561 1106 74-75 -0.4358500806459382 1106 76-77 -0.26746269164192427 1106 78-79 -0.3373155263013352 1106 80-81 -0.28373452121718046 1106 82-83 -0.3257673862479642 1106 84-85 -0.3942374527583574 1106 86-87 -0.3202320430540375 1106 88-89 -0.24074908308564247 1106 90-91 -0.28578714151834816 1106 92-93 -0.33754323354991556 1106 94-95 -0.6275003333940354 1106 96-97 -0.6711273952955175 1106 98-99 -0.9276887959437126 1106 100-101 -0.7169674496574139 1107 1 0.10473194874751357 1107 2 0.058635904585784715 1107 3 0.04986401763002135 1107 4 -0.012904503410389623 1107 5 -0.07444462376322747 1107 6 -0.16825542867636756 1107 7 -0.007262562808264761 1107 8 0.01972316968807064 1107 9 -0.09500701072090578 1107 10-11 0.11589260719238581 1107 12-13 0.054875877309370935 1107 14-15 -0.0038076666026825023 1107 16-17 -0.11376009276238364 1107 18-19 -0.07164436853151557 1107 20-21 -0.005770230289058986 1107 22-23 -0.07356184362537732 1107 24-25 -0.15773652510898728 1107 26-27 -0.02709695372512755 1107 28-29 0.037537705339502736 1107 30-31 -0.014849935703047379 1107 32-33 0.16597063985528138 1107 34-35 0.10089463155061651 1107 36-37 0.2913366336814889 1107 38-39 0.2565128891542727 1107 40-41 0.38630453466053183 1107 42-43 0.27501266172471617 1107 44-45 -0.04862375816937714 1107 46-47 -0.09347354968682708 1107 48-49 -0.3292780880866317 1107 50-51 -0.1237996638830694 1107 52-53 -0.11506369168236574 1107 54-55 0.14646352133327412 1107 56-57 0.04947877318409866 1107 58-59 -0.13884256562540287 1107 60-61 -0.08718546160703511 1107 62-63 0.15847438961389315 1107 64-65 0.0820146880472663 1107 66-67 0.04103113791619961 1107 68-69 -0.1098573082202634 1107 70-71 -0.20089452445260036 1107 72-73 -0.1313405270060457 1107 74-75 -0.179025173203339 1107 76-77 0.06519736754357197 1107 78-79 0.1903588168293453 1107 80-81 -0.006520296201273368 1107 82-83 0.2839851961782962 1107 84-85 0.3143006740591652 1107 86-87 -0.2582698261174201 1107 88-89 0.04257585211350445 1107 90-91 -0.1292829289288342 1107 92-93 -0.141583091948835 1107 94-95 0.2114732722665913 1107 96-97 0.4001524803551604 1107 98-99 0.25945650743532767 1107 100-101 0.3399003736743147 1108 1 -0.011174071143415176 1108 2 -0.09608083165199588 1108 3 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2027.0 14 2494.0 15 3558.0 16 4711.0 17 6481.0 18 6167.0 19 3985.0 20 3577.0 21 3705.0 22 3863.0 23 4180.0 24 4757.0 25 5751.0 26 6786.0 27 8478.0 28 10479.0 29 13203.0 30 16415.0 31 20499.0 32 26176.0 33 34759.0 34 47633.0 35 70014.0 36 108893.0 37 160836.0 38 109900.0 39 7076.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49220177783747 19.814271568174966 15.277513828180481 22.416012825807076 2 15.761090833767849 20.90655200245709 39.72357735191389 23.608779811861176 3 19.482005468212087 24.89522117118018 30.009742216940026 25.6130311436677 4 13.330771582276494 17.815502498421523 36.80993426108719 32.04379165821479 5 13.922227967876397 37.39282847127189 34.089331272516375 14.595612288335339 6 31.688917585415815 36.74644237664382 17.034452021724285 14.530188016216078 7 27.62532747847998 33.49308474014565 20.714495904554845 18.167091876819523 8 26.01929016093228 35.42867183013688 19.50028998094411 19.051748027986733 9 25.99729160655157 17.092733906057603 20.193101596180824 36.71687289121001 10-11 24.741827667959342 27.137078311381156 27.10000521452037 21.021088806139133 12-13 25.807589786956857 24.607811486673732 27.604257434511446 21.980341291857965 14-15 22.880282152887098 25.949509032263595 26.01443333723784 25.15577547761147 16-17 21.93634418309654 29.019950117563702 26.625393188447617 22.41831251089214 18-19 21.954135707281047 27.39379767114472 29.284394469772234 21.367672151801994 20-21 22.7929218077603 26.492295055622023 29.471135474885273 21.243647661732407 22-23 22.375937637047503 26.466399345171858 29.45498127940048 21.702681738380157 24-25 22.285121608522633 26.50065038362276 29.5421313582053 21.67209664964931 26-27 21.94900351281198 26.717656487273732 29.49040655191576 21.842933447998526 28-29 21.749530387332243 27.012192073366712 29.618953067302815 21.619324471998226 30-31 22.133955974649602 26.760481894665716 29.298307140352144 21.807254990332538 32-33 22.07980514772845 26.746617048859495 29.392479473727445 21.781098329684614 34-35 22.206754984672436 26.92287363973225 29.40671041617265 21.46366095942267 36-37 22.43305811570569 26.666466664238065 29.310855917536145 21.589619302520102 38-39 22.24102485709918 26.99850420237699 29.19298360908679 21.56748733143704 40-41 22.302201950321304 26.936518491482968 29.17803806504928 21.58324149314645 42-43 22.25975150204415 27.039508832276738 28.848104267433854 21.85263539824526 44-45 22.2616085228685 27.16871462732449 28.851532613571106 21.718144236235904 46-47 22.528733826062858 27.51297771860706 28.238501469903404 21.719786985426673 48-49 22.434120021770713 27.593950484484083 28.12136172668612 21.850567767059083 50-51 22.543511971640346 27.726163325159625 28.02023118290706 21.71009352029297 52-53 22.70443656559712 27.851241204149442 27.721035474811657 21.723286755441784 54-55 22.608215546125756 27.81773261440817 27.701879857405398 21.872171982060678 56-57 22.451787091797375 28.032699065740978 27.5502128510614 21.965300991400248 58-59 22.31562091321207 27.802907096753 27.831048124729037 22.0504238653059 60-61 22.461884157551673 27.84495676657091 27.872098189095716 21.821060886781698 62-63 22.234301440625366 27.843804753799418 28.07251626230253 21.849377543272688 64-65 22.17232867554418 27.93573565165718 27.922665082008898 21.969270590789748 66-67 22.130710794047147 28.05070896556453 27.763722904887477 22.054857335500845 68-69 22.229483654990997 27.90482904325505 27.821548742188902 22.044138559565056 70-71 22.281068023921474 28.05309121400833 27.6918978259115 21.973942936158693 72-73 22.302391557126246 27.8671687289121 27.542332932407298 22.288106781554355 74-75 22.183542224368114 28.17621984840996 27.38112924007851 22.259108687143417 76-77 22.236895861129124 28.17643412004354 27.376343840261924 22.21032617856541 78-79 22.32488375581221 28.229802795574503 27.09828794274572 22.347025505867563 80-81 22.39882349411961 28.093577643531287 27.18869948674288 22.318899375606215 82-83 22.28918674888136 28.420143551880408 27.198852499299957 22.091817199938284 84-85 22.295635602330595 28.494693165007963 26.938321570128863 22.27134966253258 86-87 22.08949680368558 28.089711081747083 27.167886861183526 22.652905253383807 88-89 22.050765235427384 28.12529462349617 27.16471489016436 22.659225250912083 90-91 21.945549136911392 28.200513110604476 27.1983618172572 22.655575935226928 92-93 21.941962260970552 28.125926280400748 27.337547827316694 22.59456363131201 94-95 21.8261371395594 28.33485228113581 27.155929753187646 22.68308082611714 96-97 21.775147421832145 28.358703602121043 27.26561071493875 22.600538261108063 98-99 21.55467718803033 28.466821706312246 26.9873263660726 22.99117473958482 100-101 21.40638403184945 28.853921220609863 26.725910604126746 23.013784143413947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 200.0 1 152.5 2 116.5 3 138.5 4 135.5 5 141.0 6 236.5 7 477.5 8 829.5 9 1059.0 10 981.5 11 868.0 12 914.0 13 937.5 14 1022.0 15 1126.0 16 1135.0 17 1167.0 18 1208.5 19 1298.0 20 1445.0 21 1651.5 22 1928.0 23 2376.0 24 2942.0 25 3621.5 26 4589.5 27 5874.0 28 6949.0 29 7939.5 30 9117.0 31 10413.0 32 11820.0 33 12935.5 34 14404.5 35 15661.5 36 17035.0 37 18593.0 38 19495.5 39 20082.0 40 20653.5 41 21436.0 42 21540.5 43 21986.0 44 22842.5 45 23493.5 46 24308.0 47 25087.5 48 26098.5 49 25990.5 50 24871.5 51 24381.0 52 24345.5 53 24060.0 54 23689.5 55 22354.0 56 19832.0 57 17728.0 58 16335.0 59 14594.0 60 12124.0 61 9979.5 62 8010.5 63 5816.0 64 4023.0 65 2833.0 66 2083.0 67 1676.0 68 1359.0 69 1171.0 70 907.5 71 575.0 72 368.5 73 204.5 74 136.5 75 94.0 76 53.5 77 30.5 78 24.5 79 11.5 80 5.5 81 3.5 82 3.5 83 4.5 84 2.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029569485433814352 2 0.004428280427286207 3 0.0 4 5.713910228756396E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011070701068215517 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.5711938929727474E-4 20-21 0.0012142059236107342 22-23 0.0021427163357836485 24-25 0.010642157801058788 26-27 0.005285366961599666 28-29 1.428477557189099E-4 30-31 0.0024998357250809234 32-33 0.0040711610379889325 34-35 0.0 36-37 0.00778520268668059 38-39 0.012142059236107342 40-41 0.0010713581678918243 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0032854983815349278 50-51 0.005356790839459121 52-53 0.0 54-55 0.002785531236518743 56-57 0.0037140416486916576 58-59 7.142387785945495E-5 60-61 0.001285629801470189 62-63 0.005356790839459121 64-65 0.0 66-67 0.0017141730686269189 68-69 7.142387785945495E-5 70-71 7.856626564540045E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.001571325312908009 80-81 9.999342900323693E-4 82-83 0.012856298014701891 84-85 0.00749950717524277 86-87 0.002999802870097108 88-89 0.0 90-91 2.856955114378198E-4 92-93 2.1427163357836486E-4 94-95 0.0 96-97 0.0019998685800647386 98-99 0.001428477557189099 100-101 3.5711938929727474E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 700046.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.55309970739533 #Duplication Level Percentage of deduplicated Percentage of total 1 83.85861032757909 57.487676751101425 2 9.806976389325234 13.445972604909695 3 2.7611385744847556 5.678538240077667 4 1.1687272972510618 3.204795157568268 5 0.638523005724408 2.1886365638445553 6 0.40339382270954316 1.6592338169732805 7 0.261534614237612 1.2550305940736333 8 0.18604033259423106 1.020291317594345 9 0.14204579816582072 0.8763911788210255 >10 0.7200664235228098 8.62995661481539 >50 0.03897821588076179 1.8221500840582143 >100 0.013131455329802616 1.5879276905182087 >500 2.0843579869503918E-4 0.10259444625001693 >1k 6.253073960851176E-4 1.0408049393943222 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3146 0.4493990394916905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2554 0.3648331681060959 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1584 0.2262708450587533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.1025646886061773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.856955114378198E-4 0.0 24-25 0.0 0.0 0.0 7.856626564540045E-4 0.0 26-27 0.0 0.0 0.0 0.0017855969464863736 0.0 28-29 1.428477557189099E-4 0.0 0.0 0.0036426177708322027 0.0 30-31 1.428477557189099E-4 0.0 0.0 0.005999605740194216 0.0 32-33 1.428477557189099E-4 0.0 0.0 0.0093565279995886 0.0 34-35 1.428477557189099E-4 0.0 0.0 0.013784808426874805 0.0 36-37 1.428477557189099E-4 0.0 0.0 0.01821308885416101 0.0 38-39 4.285432671567297E-4 0.0 0.0 0.02335560806004177 0.0 40-41 4.285432671567297E-4 0.0 0.0 0.02756961685374961 0.0 42-43 4.285432671567297E-4 0.0 0.0 0.0404259148684515 0.0 44-45 4.285432671567297E-4 0.0 0.0 0.05513923370749922 0.0 46-47 4.285432671567297E-4 0.0 0.0 0.06656705416501202 0.0 48-49 4.285432671567297E-4 0.0 0.0 0.080851829736903 0.0 50-51 4.285432671567297E-4 0.0 0.0 0.09692220225528037 0.0 52-53 4.285432671567297E-4 1.428477557189099E-4 0.0 0.13406261874219694 0.0 54-55 4.285432671567297E-4 1.428477557189099E-4 0.0 0.16420349519888694 0.0 56-57 4.285432671567297E-4 1.428477557189099E-4 0.0 0.22791359424952073 0.0 58-59 4.999671450161846E-4 1.428477557189099E-4 0.0 0.31812195198601234 0.0 60-61 5.713910228756396E-4 1.428477557189099E-4 0.0 0.39368841476131566 0.0 62-63 5.713910228756396E-4 1.428477557189099E-4 0.0 0.5070381089242707 0.0 64-65 5.713910228756396E-4 1.428477557189099E-4 0.0 0.5809618225088066 0.0 66-67 5.713910228756396E-4 1.428477557189099E-4 0.0 0.7127388771595009 0.0 68-69 5.713910228756396E-4 1.428477557189099E-4 0.0 0.8717284292746477 0.0 70-71 5.713910228756396E-4 1.428477557189099E-4 0.0 0.9759358670715925 0.0 72-73 6.428149007350946E-4 1.428477557189099E-4 0.0 1.1867791545127035 0.0 74-75 7.142387785945495E-4 1.428477557189099E-4 0.0 1.3841233290383776 0.0 76-77 7.142387785945495E-4 1.428477557189099E-4 0.0 1.5066152795673426 0.0 78-79 7.142387785945495E-4 1.428477557189099E-4 0.0 1.6320356090885455 0.0 80-81 8.570865343134594E-4 1.428477557189099E-4 0.0 1.8704485133834061 0.0 82-83 8.570865343134594E-4 1.428477557189099E-4 0.0 2.06650705810761 0.0 84-85 8.570865343134594E-4 1.428477557189099E-4 0.0 2.372201255346077 0.0 86-87 8.570865343134594E-4 1.428477557189099E-4 0.0 2.843813120852058 0.0 88-89 8.570865343134594E-4 1.428477557189099E-4 0.0 3.502127003082655 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2070 0.0 41.09648 1 GTATCAA 3185 0.0 35.065525 1 CACGACT 95 3.4883058E-5 30.000767 7 TAAGGTG 320 0.0 29.688261 5 ATCAACG 4035 0.0 27.072412 3 TATCAAC 4105 0.0 26.845999 2 TCAACGC 4080 0.0 26.657413 4 CAACGCA 4175 0.0 26.054558 5 ACGACTC 130 1.0011785E-5 25.577576 8 AACGCAG 4305 0.0 25.267776 6 GGACCGT 95 0.0012258929 25.000639 6 GTAAGGT 355 0.0 22.743809 4 ACGCAGA 4820 0.0 22.469454 7 CGCAGAG 4870 0.0 21.946146 8 GGTAAGG 370 0.0 21.821766 3 GTACATG 2490 0.0 21.758425 1 GAGTACT 2955 0.0 21.6176 12-13 GACGCAT 55 0.0028772494 21.591461 72-73 GGACCAT 330 1.8189894E-12 21.591461 6 TACATGG 2455 0.0 21.090153 2 >>END_MODULE