FastQCFastQC Report
Thu 26 May 2016
SRR937020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760767
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.5021248292841303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.3793539940612566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17790.2338429506011696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.10449980085887006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.044.8990331
GTATCAA32100.039.0923731
TCAACGC40250.030.830614
ATCAACG40300.030.792363
TATCAAC40800.030.4150032
CAACGCA41300.030.0467835
AACGCAG42600.029.345356
CGCTAAT856.369569E-427.967822
CGCAGAG47100.026.4372588
ACGCAGA47400.026.3684627
GAGTACT29950.025.06994412-13
GCAGAGT50200.024.6153269
TGGTATC10050.023.1812862
TACATGG24750.023.0522612
AGAGTAC43000.022.32347110-11
AGTACTT32050.022.01869412-13
GTACTTT33350.022.01825714-15
GTGGTAT10900.021.8040261
GTATAGG1754.022131E-621.7292691
GTACATG26900.021.5576151
Produced by FastQC (version 0.11.5)