##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937020_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 760767 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74879693782722 33.0 31.0 34.0 30.0 34.0 2 31.84415202026376 33.0 31.0 34.0 30.0 34.0 3 31.85544851445975 33.0 31.0 34.0 30.0 34.0 4 35.45037179583236 37.0 35.0 37.0 33.0 37.0 5 35.44840141593944 37.0 35.0 37.0 33.0 37.0 6 35.511027686532145 37.0 35.0 37.0 33.0 37.0 7 35.45746989551334 37.0 35.0 37.0 33.0 37.0 8 35.413622041965546 37.0 35.0 37.0 33.0 37.0 9 37.04345745806535 39.0 37.0 39.0 33.0 39.0 10-11 36.917223013090734 39.0 37.0 39.0 33.0 39.0 12-13 36.83350355627938 39.0 37.0 39.0 33.0 39.0 14-15 37.99501029881685 40.0 38.0 41.0 33.0 41.0 16-17 37.91854141938333 40.0 38.0 41.0 33.0 41.0 18-19 37.875279816290664 40.0 38.0 41.0 33.0 41.0 20-21 37.81753677538589 40.0 38.0 41.0 32.5 41.0 22-23 37.705775224214506 40.0 37.5 41.0 32.0 41.0 24-25 37.6662723277955 40.0 37.0 41.0 32.0 41.0 26-27 37.68445397868204 40.0 37.0 41.0 32.0 41.0 28-29 37.597725059052244 40.0 37.0 41.0 32.0 41.0 30-31 37.432612087537976 40.0 37.0 41.0 32.0 41.0 32-33 37.218820611304125 40.0 36.5 41.0 31.0 41.0 34-35 37.04819478237095 40.0 36.0 41.0 31.0 41.0 36-37 36.860542715443756 39.0 36.0 41.0 30.5 41.0 38-39 36.70505161238592 39.0 36.0 41.0 30.0 41.0 40-41 36.5138235491287 39.0 35.0 41.0 30.0 41.0 42-43 36.292372697553915 39.0 35.0 41.0 30.0 41.0 44-45 36.03675041109827 39.0 35.0 40.5 29.5 41.0 46-47 35.68137156317243 39.0 35.0 40.0 27.5 41.0 48-49 35.43293413094942 39.0 35.0 40.0 27.0 41.0 50-51 34.73672425328648 38.0 34.0 39.5 25.0 40.5 52-53 34.92414563197405 38.5 34.5 40.0 26.5 40.5 54-55 35.314639041914276 39.0 35.0 40.5 27.0 41.0 56-57 35.186133205041756 39.0 35.0 41.0 26.0 41.0 58-59 34.96884788640938 38.5 34.5 40.0 26.0 41.0 60-61 34.68963756314351 38.0 34.0 40.0 26.0 41.0 62-63 34.373016311170176 38.0 34.0 40.0 25.0 41.0 64-65 34.04165401496122 37.0 34.0 40.0 24.0 41.0 66-67 33.67028341134671 37.0 33.5 39.5 23.0 41.0 68-69 33.27482330332414 36.0 33.0 39.0 22.0 41.0 70-71 32.85566408637598 36.0 33.0 39.0 21.5 40.5 72-73 32.46585748330304 35.0 32.0 38.5 20.0 40.0 74-75 32.08465206824165 35.0 32.0 37.5 19.5 39.5 76-77 31.63257409956005 35.0 31.5 37.0 18.0 39.0 78-79 31.179265136368954 35.0 31.0 36.5 15.0 39.0 80-81 30.752664087690448 35.0 31.0 36.0 9.5 38.5 82-83 30.364762141365226 34.5 31.0 35.5 7.0 37.0 84-85 30.007890063580568 34.0 30.0 35.0 6.0 37.0 86-87 29.64273095441837 34.0 30.0 35.0 2.0 36.0 88-89 29.283178686772693 34.0 29.5 35.0 2.0 36.0 90-91 28.919229540713516 34.0 29.0 35.0 2.0 36.0 92-93 28.54950793081193 34.0 29.0 35.0 2.0 35.0 94-95 28.176924077937137 34.0 29.0 35.0 2.0 35.0 96-97 27.710473114632997 34.0 28.0 35.0 2.0 35.0 98-99 27.15221743319571 34.0 27.0 35.0 2.0 35.0 100-101 25.006054416135296 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3787849664029679 1101 2 0.19112164528921483 1101 3 0.2969180131060263 1101 4 0.42525061069720493 1101 5 0.3413060787633668 1101 6 0.4132074454294212 1101 7 0.35744254405202724 1101 8 0.2674026715566171 1101 9 0.38867993187425043 1101 10-11 0.39471587656896645 1101 12-13 0.43128254661159104 1101 14-15 0.4652696591350036 1101 16-17 0.38503996320237377 1101 18-19 0.4988978887349518 1101 20-21 0.4480509757417295 1101 22-23 0.4890495791710947 1101 24-25 0.49240768321274686 1101 26-27 0.49403494120753066 1101 28-29 0.5192807510430413 1101 30-31 0.49897911007195717 1101 32-33 0.36491503859524954 1101 34-35 0.5977880771978121 1101 36-37 0.4938224498853856 1101 38-39 0.5098759526879135 1101 40-41 0.44050662015215636 1101 42-43 0.5216164363800928 1101 44-45 0.6493284128464367 1101 46-47 0.8304307326606946 1101 48-49 0.8927971725293773 1101 50-51 1.1212793186903625 1101 52-53 1.0757049858914485 1101 54-55 1.1529342707524322 1101 56-57 1.1184814437134918 1101 58-59 1.1754939930814317 1101 60-61 1.1783542069033004 1101 62-63 1.2073837906416074 1101 64-65 1.0098606877899883 1101 66-67 1.2015161765319178 1101 68-69 1.1565423346941444 1101 70-71 1.0365980790303198 1101 72-73 0.9478079388573022 1101 74-75 0.9517993954949766 1101 76-77 0.9501394840220883 1101 78-79 0.9194022672541315 1101 80-81 1.0073841134095964 1101 82-83 0.9572869611342512 1101 84-85 0.9147698652514364 1101 86-87 1.0574057581492227 1101 88-89 1.3963969841537924 1101 90-91 1.1507135148571948 1101 92-93 1.0779156463984343 1101 94-95 1.0231302146399663 1101 96-97 1.0832384491125246 1101 98-99 0.923548597358959 1101 100-101 1.1099124550256363 1102 1 0.20504142881503284 1102 2 0.23146404718767855 1102 3 0.18173876062700245 1102 4 0.2302340838728938 1102 5 0.22227056727934524 1102 6 0.12284385529466135 1102 7 0.05186580026018106 1102 8 0.11730520566967328 1102 9 0.2542193843702236 1102 10-11 0.19946627914545445 1102 12-13 0.08222754195018211 1102 14-15 -0.09768059088618486 1102 16-17 0.14107861054536386 1102 18-19 -0.1596324951958863 1102 20-21 -0.00849195162499683 1102 22-23 -0.08029951821004033 1102 24-25 -0.10431661545663928 1102 26-27 0.0470902848807242 1102 28-29 -0.24222446126307773 1102 30-31 -0.157707897301556 1102 32-33 -0.04795893377529126 1102 34-35 -0.06902690712288972 1102 36-37 -0.0073005266380477 1102 38-39 0.12393222864350406 1102 40-41 -0.20249278657723124 1102 42-43 -0.05622914375381782 1102 44-45 0.04607813739936262 1102 46-47 0.044198848602725604 1102 48-49 0.33827984055209015 1102 50-51 0.5389842089352967 1102 52-53 0.5070551003079231 1102 54-55 0.15204436482820682 1102 56-57 0.35617107842829654 1102 58-59 0.40019523894972764 1102 60-61 0.16691255930604854 1102 62-63 0.08720411439786346 1102 64-65 0.26613240573218633 1102 66-67 -0.02807955191964595 1102 68-69 -0.057310534193042884 1102 70-71 0.05615482112710879 1102 72-73 0.25313042381365136 1102 74-75 0.44773125412706705 1102 76-77 0.28807066463061304 1102 78-79 0.2115456692373492 1102 80-81 0.061774315969135074 1102 82-83 0.12613601613891134 1102 84-85 0.04903971862884049 1102 86-87 0.11334270195801821 1102 88-89 0.2933585910696337 1102 90-91 0.2987980982964693 1102 92-93 0.37569511923511456 1102 94-95 0.23929260128290153 1102 96-97 -0.19748449350973019 1102 98-99 -0.18279516058663248 1102 100-101 -0.2885535166668802 1103 1 0.21464086012062822 1103 2 0.1990340860005304 1103 3 0.15056774125042338 1103 4 0.30061408623340924 1103 5 0.04622017162160574 1103 6 0.19019588554182576 1103 7 0.05069843018690534 1103 8 0.25216256403533066 1103 9 0.28633935826889 1103 10-11 0.2474074751673072 1103 12-13 0.05091475595808248 1103 14-15 0.4129413914859157 1103 16-17 0.3586306741653402 1103 18-19 0.34693828568719454 1103 20-21 0.20281865021159007 1103 22-23 0.21906782283159743 1103 24-25 0.3090344760106447 1103 26-27 0.3357250791563402 1103 28-29 0.2937225329455089 1103 30-31 0.3114761781698405 1103 32-33 0.4912609021552896 1103 34-35 0.42420051501739664 1103 36-37 0.3925874845187849 1103 38-39 0.6767267267422028 1103 40-41 0.5872893676512803 1103 42-43 0.6371330584175823 1103 44-45 0.5880441079851622 1103 46-47 0.39624028786624876 1103 48-49 0.5097146814953746 1103 50-51 0.5579958582168985 1103 52-53 0.5110537328366433 1103 54-55 0.537473876974687 1103 56-57 0.5436258860801715 1103 58-59 0.6778051352091268 1103 60-61 0.7283755093811308 1103 62-63 0.8112916868243261 1103 64-65 0.9415713056775061 1103 66-67 0.772171965118801 1103 68-69 0.7961824866839535 1103 70-71 0.7290744808329706 1103 72-73 0.714466123089359 1103 74-75 0.7516476755849659 1103 76-77 0.7792740122220039 1103 78-79 0.900207867203239 1103 80-81 0.7316192002292432 1103 82-83 0.8930415154626381 1103 84-85 0.9411502368977409 1103 86-87 0.9065533698619248 1103 88-89 0.5719920558592833 1103 90-91 0.5670256221831274 1103 92-93 0.7777547371842601 1103 94-95 0.939912711521707 1103 96-97 1.0206146264519589 1103 98-99 1.16116761651962 1103 100-101 1.2967560704191996 1104 1 0.24392514699671608 1104 2 0.08991963081278698 1104 3 0.04820155369529999 1104 4 0.06908530046643335 1104 5 0.27040985135799644 1104 6 0.05286202663712203 1104 7 0.14391177523395982 1104 8 0.03293403753305313 1104 9 0.05649892757517705 1104 10-11 0.2799040164439859 1104 12-13 0.14655113958052368 1104 14-15 0.23124656123602705 1104 16-17 0.2962663072169107 1104 18-19 0.3104291100870995 1104 20-21 0.2692673593413346 1104 22-23 0.1536565160052703 1104 24-25 0.020751711745717216 1104 26-27 0.04209685906560168 1104 28-29 0.2535429455533915 1104 30-31 0.40034067779927796 1104 32-33 0.10912715111295057 1104 34-35 0.19240035580018144 1104 36-37 0.30154212135497716 1104 38-39 0.3379736533949469 1104 40-41 0.17905739144283217 1104 42-43 0.3940064927602336 1104 44-45 0.4194142776880341 1104 46-47 0.3958697257024042 1104 48-49 0.3738875463831661 1104 50-51 0.4276588828114072 1104 52-53 0.5335365079221575 1104 54-55 0.7944456412542067 1104 56-57 0.8082016890149575 1104 58-59 0.5924015058468655 1104 60-61 0.7069116728990963 1104 62-63 0.5484755174323297 1104 64-65 0.647889412040449 1104 66-67 0.6389859978742649 1104 68-69 0.6653585848661763 1104 70-71 0.8154768675452857 1104 72-73 0.9619531276503182 1104 74-75 0.9949545843579735 1104 76-77 0.8811618611322558 1104 78-79 0.7881533898418809 1104 80-81 0.7746486382371565 1104 82-83 0.7061970304839313 1104 84-85 0.7820502520980881 1104 86-87 0.9797168543313752 1104 88-89 0.7102911798211302 1104 90-91 0.7847079211628127 1104 92-93 0.6443798390566506 1104 94-95 0.5919200592155072 1104 96-97 0.4995896248846883 1104 98-99 0.40601173565943327 1104 100-101 0.19968681780400743 1105 1 0.057576440770336745 1105 2 0.10644574825633768 1105 3 -0.022526110304536218 1105 4 0.06012721811385546 1105 5 0.07922172133194749 1105 6 0.10293461799412285 1105 7 0.01353563874161523 1105 8 0.07036234713261535 1105 9 0.01308826642751626 1105 10-11 0.15751292922109172 1105 12-13 0.13422090553775234 1105 14-15 0.3944020714863328 1105 16-17 0.36925408509765134 1105 18-19 0.2650003062485098 1105 20-21 0.2764956051016938 1105 22-23 0.3017943675070356 1105 24-25 0.24271651780838255 1105 26-27 0.3858401018914108 1105 28-29 0.3472939108639821 1105 30-31 0.20960045189085008 1105 32-33 0.17294576539027418 1105 34-35 -0.05383879410869241 1105 36-37 0.1625013569319549 1105 38-39 0.35714600895872906 1105 40-41 0.5300134350594234 1105 42-43 0.6382274999781856 1105 44-45 0.5327443160945293 1105 46-47 0.418558386005742 1105 48-49 0.4437914786724235 1105 50-51 0.3012932900849563 1105 52-53 0.42244084178062735 1105 54-55 0.5168274614953603 1105 56-57 0.5508473093364117 1105 58-59 0.23154008841051876 1105 60-61 0.4349309070345413 1105 62-63 0.5604144789125698 1105 64-65 0.6873616059468901 1105 66-67 0.5510938243551777 1105 68-69 0.3698963169268481 1105 70-71 0.3421915165840943 1105 72-73 0.45446746573040997 1105 74-75 0.38529530038676185 1105 76-77 0.4121496184197113 1105 78-79 0.4357197774694299 1105 80-81 0.3554721640148095 1105 82-83 0.40668303219317536 1105 84-85 0.38714297339876325 1105 86-87 0.7036174299139049 1105 88-89 0.7803907483916461 1105 90-91 0.5815653846869431 1105 92-93 0.8223110368655249 1105 94-95 0.7226771716239959 1105 96-97 0.5451471368030596 1105 98-99 0.5982398269700937 1105 100-101 0.7965731119380095 1106 1 0.323723575197036 1106 2 0.3102716415983622 1106 3 0.2664161799818423 1106 4 0.260816910739031 1106 5 0.23324402320371007 1106 6 0.1595627270415818 1106 7 0.33409789209956386 1106 8 0.2627521954364411 1106 9 0.3804401001771964 1106 10-11 0.445966460954196 1106 12-13 0.31969572542116964 1106 14-15 0.2961593395070423 1106 16-17 0.5464471628283718 1106 18-19 0.42531763726476157 1106 20-21 0.5180034252385468 1106 22-23 0.21930504778653415 1106 24-25 0.1872786263489843 1106 26-27 -0.016068771791658776 1106 28-29 0.20399261836914206 1106 30-31 0.2790811006725704 1106 32-33 0.20586229629068242 1106 34-35 0.21511417645718467 1106 36-37 0.20550984684903995 1106 38-39 -0.16124126883714496 1106 40-41 0.06901908293603753 1106 42-43 -0.09828864715623808 1106 44-45 -0.12020543228533143 1106 46-47 -0.2545096600461747 1106 48-49 -0.09334059474996792 1106 50-51 -0.01554217930427626 1106 52-53 0.08571335182732298 1106 54-55 0.10429035204965231 1106 56-57 0.13410144568194937 1106 58-59 0.28105857072470286 1106 60-61 0.18003200764639615 1106 62-63 0.3968568597097928 1106 64-65 0.3141039396784677 1106 66-67 0.25902890997843997 1106 68-69 0.3547719550282906 1106 70-71 0.23576886715068923 1106 72-73 0.017512793560676698 1106 74-75 0.4074184132356855 1106 76-77 0.22689202455996593 1106 78-79 0.26040027419293565 1106 80-81 0.6612788871602433 1106 82-83 0.6744322085444985 1106 84-85 0.5600910925834413 1106 86-87 0.6923126255983512 1106 88-89 0.6409022251665633 1106 90-91 0.6863656272560021 1106 92-93 0.7751548875588377 1106 94-95 1.0193332485220061 1106 96-97 1.0583820848498355 1106 98-99 0.934539653189276 1106 100-101 1.100234728099629 1107 1 0.2729795503282624 1107 2 0.2578013261521974 1107 3 0.34445598021625656 1107 4 0.29415788816348254 1107 5 0.3989806587844029 1107 6 0.3484545886325776 1107 7 0.34679982350658634 1107 8 0.3145791332135417 1107 9 0.3496662633530647 1107 10-11 0.2797011090711976 1107 12-13 0.3456346746134429 1107 14-15 0.31476951634034833 1107 16-17 0.5068889912949572 1107 18-19 0.5867672117436911 1107 20-21 0.6433633345286651 1107 22-23 0.7746641926675437 1107 24-25 0.5864912849638841 1107 26-27 0.5998602400821653 1107 28-29 0.5344657876093208 1107 30-31 0.5453566446770566 1107 32-33 0.5177125485456884 1107 34-35 0.5079720473230012 1107 36-37 0.4523216238454211 1107 38-39 0.29761441072255224 1107 40-41 0.48187007411985405 1107 42-43 0.3662395850202884 1107 44-45 0.36898454885924536 1107 46-47 0.1629054905424141 1107 48-49 0.1574047041339881 1107 50-51 0.13010005175112127 1107 52-53 0.1986547980893647 1107 54-55 -0.042600413888280286 1107 56-57 0.22299267026534864 1107 58-59 0.10576682124704462 1107 60-61 0.20465553615884602 1107 62-63 0.18623029904119193 1107 64-65 0.16319684086585795 1107 66-67 0.15108797332247548 1107 68-69 0.3539948057646711 1107 70-71 0.21163901952291297 1107 72-73 0.04222231809821153 1107 74-75 0.20986006334015528 1107 76-77 0.22791123670897662 1107 78-79 0.22421824290312387 1107 80-81 0.23781853451283652 1107 82-83 0.24608282261981884 1107 84-85 0.4155922901577185 1107 86-87 0.5627325410037436 1107 88-89 0.5935566358071362 1107 90-91 0.685445151283929 1107 92-93 0.680622960738269 1107 94-95 0.7432382324694125 1107 96-97 0.9377487719007469 1107 98-99 1.1486092398987502 1107 100-101 0.896147050374509 1108 1 0.0816533280838101 1108 2 0.28852156357753245 1108 3 0.17118467091723844 1108 4 0.025697609805618526 1108 5 0.3030726611019716 1108 6 0.29953563253708637 1108 7 0.1418474878098408 1108 8 0.22425246496292317 1108 9 -0.001305154738112435 1108 10-11 -0.15571240533229513 1108 12-13 0.2710391021624545 1108 14-15 0.2534447113117366 1108 16-17 0.2699200825094721 1108 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-3.1427847508876035 2316 80-81 -2.818164752984938 2316 82-83 -3.4231712062156383 2316 84-85 -3.288168222561403 2316 86-87 -3.3098725762014674 2316 88-89 -3.1122659775984154 2316 90-91 -3.7745700673187166 2316 92-93 -3.8359267703398316 2316 94-95 -3.917830305166671 2316 96-97 -4.07138338828204 2316 98-99 -4.363165205231397 2316 100-101 -4.308082802452418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1665.0 3 605.0 4 627.0 5 680.0 6 686.0 7 830.0 8 1054.0 9 1378.0 10 1673.0 11 2052.0 12 2563.0 13 3175.0 14 3592.0 15 4429.0 16 5348.0 17 7394.0 18 6590.0 19 4462.0 20 4243.0 21 4521.0 22 4888.0 23 5321.0 24 6010.0 25 6893.0 26 8035.0 27 9406.0 28 11251.0 29 13623.0 30 16543.0 31 20657.0 32 25963.0 33 34042.0 34 46958.0 35 67963.0 36 103364.0 37 153755.0 38 141026.0 39 27482.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.486464843570225 18.615360137593505 15.163556655415311 22.734618363420957 2 15.191477836009875 19.693567091530166 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25.738610282870344 30.573724242515908 21.402830150214452 22-23 21.979417123117898 25.520374128929312 30.509614569094918 21.990594178857876 24-25 21.850823536216797 25.648791940926774 30.65357054121099 21.84681398164544 26-27 21.617507586318403 25.914548182734148 30.53357599036207 21.934368240585375 28-29 21.63887252994563 26.053223821893173 30.56767870100642 21.740224947154772 30-31 21.78585372073672 25.90003194329605 30.415473174861283 21.898641161105946 32-33 21.57676621280987 25.88653251435351 30.465326458109445 22.071374814727175 34-35 21.95205474950079 25.927006800306557 30.38710347429542 21.73383497589723 36-37 22.031797196251855 25.730055107427933 30.347471263159004 21.89067643316121 38-39 21.68836640382478 26.086942233281867 30.309435688525465 21.915255674367888 40-41 21.989791022251787 26.034602203488088 30.19207233309189 21.783534441168236 42-43 21.870760639808186 25.968677372201366 30.012501183077262 22.14806080491319 44-45 21.82923679269031 26.184318367491805 30.007683273008578 21.97876156680931 46-47 22.127695373500817 26.278478550058626 29.47493782435182 22.118888252088734 48-49 22.088358428686913 26.397770665054253 29.38226653434372 22.131604371915113 50-51 22.05255644474357 26.58989094886059 29.283504043410137 22.07404856298571 52-53 22.211778748509616 26.733481528416252 28.92623854851198 22.12850117456215 54-55 22.077578896857272 26.634435249032073 28.952139302828524 22.335846551282135 56-57 21.921954688838248 26.936195358455368 28.913719223819417 22.228130728886967 58-59 22.039025050725204 26.6391618953973 29.096253334849447 22.22555971902805 60-61 21.97332583182602 26.529045555882725 29.429197513986455 22.0684310983048 62-63 21.83657586525581 26.487798272840692 29.522618845729625 22.153007016173877 64-65 21.896147394877566 26.56211385875709 29.313477019502272 22.228261726863074 66-67 21.777573838774824 26.781084336082127 29.008475155671494 22.432866669471558 68-69 21.89780877908674 26.681422097375325 29.233243643460398 22.18752548007754 70-71 21.961264748316875 26.502450874015736 29.020268537406242 22.516015840261147 72-73 21.85707515887731 26.637361423950857 28.957965949744224 22.547597467427604 74-75 21.816735686583844 26.838624860316834 28.81298889108 22.531650562019326 76-77 21.938871771138267 26.826842356802 28.70284118138556 22.531444690674174 78-79 21.87491371341942 26.833844588171406 28.679029698361834 22.612212000047336 80-81 21.898340712912027 26.771690831169447 28.719912760488604 22.610055695429924 82-83 21.970511295763405 26.83620666418155 28.794713150131784 22.398568889923258 84-85 21.954913412585988 26.82536917560368 28.509723623623294 22.709993788187038 86-87 21.985074204909825 26.69200540963096 28.57820986448967 22.74471052096955 88-89 22.02550390028321 26.558222814897814 28.55409731934847 22.86217596547051 90-91 21.743272974988262 26.502328370614002 28.98147429718189 22.772924357215846 92-93 21.790185437613964 26.53130200863898 28.789812602866554 22.8886999508805 94-95 21.72526061780503 26.58778180871616 28.7830254474988 22.90393212598001 96-97 21.63942400925582 26.49248457637202 28.991687510887747 22.87640390348441 98-99 21.56395128260259 26.819094284588978 28.704023334273593 22.912931098534838 100-101 21.547866570983736 26.87694986315192 28.600623956208615 22.97455960965573 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 139.0 2 140.5 3 179.5 4 155.5 5 171.0 6 283.0 7 605.5 8 1111.5 9 1362.0 10 1185.5 11 987.0 12 974.0 13 1018.5 14 1073.5 15 1128.0 16 1201.5 17 1264.0 18 1353.0 19 1479.5 20 1618.0 21 1877.0 22 2208.0 23 2676.5 24 3303.5 25 4050.0 26 5278.5 27 6736.5 28 7725.5 29 8601.0 30 9753.5 31 11364.5 32 12970.0 33 14131.5 34 15165.5 35 16558.5 36 18428.5 37 20014.0 38 20920.0 39 21485.0 40 22172.0 41 23088.5 42 23687.5 43 24108.0 44 25111.5 45 26132.5 46 26867.5 47 27447.0 48 28186.5 49 28435.0 50 27521.5 51 26693.0 52 26705.5 53 26433.0 54 25472.0 55 23645.0 56 21591.0 57 19365.0 58 17534.5 59 15851.5 60 13092.5 61 10637.5 62 8339.0 63 6114.5 64 4338.0 65 3058.0 66 2209.0 67 1702.0 68 1326.5 69 1015.5 70 757.5 71 474.5 72 305.0 73 215.0 74 137.5 75 73.5 76 44.5 77 29.5 78 20.5 79 11.0 80 8.0 81 7.5 82 7.5 83 5.5 84 4.0 85 5.5 86 3.5 87 2.5 88 2.0 89 1.5 90 1.0 91 0.5 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0349647132433452 2 0.027472274691199804 3 0.024317563721875424 4 0.0482407885725853 5 0.09332686617584622 6 0.11606707441305945 7 0.061253971321048366 8 0.08780612197952856 9 0.07505583181184251 10-11 0.07788192705519562 12-13 0.07466149294067698 14-15 0.06848351729241674 16-17 0.05422159478526276 18-19 0.06559169890386939 20-21 0.05967661583638617 22-23 0.03687068445397868 24-25 0.011041488392635327 26-27 0.0029575415337416053 28-29 0.007229545971368369 30-31 0.005783636777094695 32-33 0.008478285730059269 34-35 0.008675455165642044 36-37 0.009332686617584622 38-39 0.005717913631900437 40-41 0.00841256258486501 42-43 0.005126405325152116 44-45 0.003088987824130121 46-47 0.0024974795173818 48-49 2.628925807770316E-4 50-51 0.0030232646789358636 52-53 0.007623884842533917 54-55 0.016036447427398928 56-57 0.012290228151326228 58-59 0.006638037664620048 60-61 0.00407483500204399 62-63 0.012618843877297519 64-65 0.004600620163598053 66-67 0.02510624146420652 68-69 0.04850368115336233 70-71 0.05599611970550773 72-73 0.005060682179957859 74-75 0.015379215975456348 76-77 0.024383286867069684 78-79 0.028918183885473476 80-81 0.07374136890795736 82-83 0.03134994025766102 84-85 0.015707831701427637 86-87 0.03568766784048204 88-89 0.04909518946011065 90-91 0.04784644970141975 92-93 0.0494895283312762 94-95 0.016102170572593187 96-97 0.021622914768910848 98-99 0.044757461877289635 100-101 0.08116808431490852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 760767.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.29946810804225 #Duplication Level Percentage of deduplicated Percentage of total 1 85.30048063466604 60.81878898612047 2 9.089576830650643 12.961639867051506 3 2.4273575529865603 5.192079073079421 4 1.0680378071793184 3.0460211028466073 5 0.571492904537817 2.0373570060533255 6 0.34531111350268806 1.477229923472248 7 0.24167944525955803 1.2062131129757239 8 0.16022620697481524 0.9139234667418733 9 0.1333761959230072 0.8558686644826002 >10 0.6227781384300866 7.73013728856822 >50 0.02860933867556335 1.3689063128974877 >100 0.010335575802972159 1.1706517350275676 >500 1.845638527524581E-4 0.10461647103910927 >1k 5.536915582573743E-4 1.1165669896438266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3820 0.5021248292841303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2886 0.3793539940612566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1779 0.2338429506011696 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 795 0.10449980085887006 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.314462903885158E-4 9 0.0 0.0 0.0 0.0 1.314462903885158E-4 10-11 0.0 0.0 0.0 1.314462903885158E-4 1.314462903885158E-4 12-13 0.0 0.0 0.0 1.314462903885158E-4 1.314462903885158E-4 14-15 0.0 0.0 0.0 1.314462903885158E-4 1.314462903885158E-4 16-17 0.0 0.0 0.0 1.971694355827737E-4 1.314462903885158E-4 18-19 6.57231451942579E-5 0.0 0.0 2.628925807770316E-4 1.314462903885158E-4 20-21 1.314462903885158E-4 0.0 0.0 3.943388711655474E-4 1.314462903885158E-4 22-23 1.314462903885158E-4 0.0 0.0 0.0010515703231081263 1.971694355827737E-4 24-25 1.314462903885158E-4 0.0 0.0 0.001314462903885158 2.628925807770316E-4 26-27 1.314462903885158E-4 0.0 0.0 0.0017088017750507053 2.628925807770316E-4 28-29 1.314462903885158E-4 0.0 0.0 0.002694648952964574 2.628925807770316E-4 30-31 1.314462903885158E-4 0.0 0.0 0.006375145083843016 2.628925807770316E-4 32-33 1.314462903885158E-4 0.0 0.0 0.010647149521469779 3.943388711655474E-4 34-35 1.971694355827737E-4 0.0 0.0 0.013867583635988417 3.943388711655474E-4 36-37 2.628925807770316E-4 0.0 0.0 0.016759402024535765 3.943388711655474E-4 38-39 2.628925807770316E-4 0.0 0.0 0.022871654527601752 3.943388711655474E-4 40-41 2.628925807770316E-4 0.0 0.0 0.02727510525561703 3.943388711655474E-4 42-43 2.628925807770316E-4 0.0 0.0 0.04055118058485713 3.943388711655474E-4 44-45 2.628925807770316E-4 0.0 0.0 0.05593039656031347 4.600620163598053E-4 46-47 2.628925807770316E-4 0.0 0.0 0.06815490156644544 5.257851615540632E-4 48-49 2.628925807770316E-4 0.0 0.0 0.08097091487932573 5.257851615540632E-4 50-51 2.628925807770316E-4 0.0 0.0 0.09819037892022131 5.257851615540632E-4 52-53 2.628925807770316E-4 0.0 0.0 0.1336151541799263 5.257851615540632E-4 54-55 2.628925807770316E-4 6.57231451942579E-5 0.0 0.16319056951734234 5.257851615540632E-4 56-57 2.628925807770316E-4 1.314462903885158E-4 0.0 0.22023825954595821 5.257851615540632E-4 58-59 2.628925807770316E-4 1.314462903885158E-4 0.0 0.3004204966829529 5.257851615540632E-4 60-61 2.628925807770316E-4 1.314462903885158E-4 0.0 0.3710728777667801 6.57231451942579E-4 62-63 2.628925807770316E-4 1.314462903885158E-4 0.0 0.47327236854385113 6.57231451942579E-4 64-65 2.628925807770316E-4 1.314462903885158E-4 0.0 0.538732621157332 6.57231451942579E-4 66-67 2.628925807770316E-4 1.314462903885158E-4 0.0 0.6691273412227396 6.57231451942579E-4 68-69 2.628925807770316E-4 1.314462903885158E-4 0.0 0.8311348941265855 6.57231451942579E-4 70-71 2.628925807770316E-4 1.314462903885158E-4 0.0 0.9264334546582593 6.57231451942579E-4 72-73 2.628925807770316E-4 1.314462903885158E-4 0.0 1.1135472490263116 7.22954597136837E-4 74-75 3.2861572597128947E-4 1.314462903885158E-4 0.0 1.2938915594393552 7.886777423310948E-4 76-77 3.943388711655474E-4 1.314462903885158E-4 0.0 1.4002973315088587 7.886777423310948E-4 78-79 3.943388711655474E-4 1.314462903885158E-4 0.0 1.513012525517011 7.886777423310948E-4 80-81 5.257851615540632E-4 1.314462903885158E-4 0.0 1.7302932435292278 7.886777423310948E-4 82-83 5.257851615540632E-4 1.314462903885158E-4 0.0 1.9136608186212074 8.544008875253526E-4 84-85 5.257851615540632E-4 1.314462903885158E-4 0.0 2.1925241236804434 9.201240327196106E-4 86-87 5.257851615540632E-4 1.314462903885158E-4 0.0 2.6385213869686774 9.201240327196106E-4 88-89 5.257851615540632E-4 1.314462903885158E-4 0.0 3.243042876465462 9.201240327196106E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1895 0.0 44.899033 1 GTATCAA 3210 0.0 39.092373 1 TCAACGC 4025 0.0 30.83061 4 ATCAACG 4030 0.0 30.79236 3 TATCAAC 4080 0.0 30.415003 2 CAACGCA 4130 0.0 30.046783 5 AACGCAG 4260 0.0 29.34535 6 CGCTAAT 85 6.369569E-4 27.96782 2 CGCAGAG 4710 0.0 26.437258 8 ACGCAGA 4740 0.0 26.368462 7 GAGTACT 2995 0.0 25.069944 12-13 GCAGAGT 5020 0.0 24.615326 9 TGGTATC 1005 0.0 23.181286 2 TACATGG 2475 0.0 23.052261 2 AGAGTAC 4300 0.0 22.323471 10-11 AGTACTT 3205 0.0 22.018694 12-13 GTACTTT 3335 0.0 22.018257 14-15 GTGGTAT 1090 0.0 21.804026 1 GTATAGG 175 4.022131E-6 21.729269 1 GTACATG 2690 0.0 21.557615 1 >>END_MODULE