FastQCFastQC Report
Thu 26 May 2016
SRR937020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760767
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.44402556893240636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.3643691169569658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.23752344673204803No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.11238657828218102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.043.6593061
GTATCAA34200.038.3368071
GACCGGT500.001615796437.998457
CGCAGTA550.00257793334.5485889
TAGACCG902.412571E-531.6653775
TATCAAC42050.030.8370292
ATCAACG42900.030.2260383
TCAACGC43600.029.8496994
CAACGCA44000.029.5783375
CTATGCG650.00584025829.233429
AACGCAG45050.028.8889456
TCGTACC908.9400203E-426.3912819
ACGCAGA50600.025.6264237
CGCAGAG51750.025.0602448
GTAAGGT3300.023.0293624
GCAGAGT55500.023.024619
GAGTACT32700.022.15487712-13
AGTACTT33450.021.94217312-13
CGATCCG550.002877373521.59144670-71
AGAGTAC47700.021.51138510-11
Produced by FastQC (version 0.11.5)