##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937020_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 760767 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.179357148772226 33.0 31.0 34.0 31.0 34.0 2 32.31772934420131 33.0 31.0 34.0 31.0 34.0 3 32.439526162412406 34.0 31.0 34.0 31.0 34.0 4 36.09445467534738 37.0 35.0 37.0 35.0 37.0 5 35.9280029233655 37.0 35.0 37.0 35.0 37.0 6 36.01572229079337 37.0 35.0 37.0 35.0 37.0 7 35.97524472013113 37.0 35.0 37.0 35.0 37.0 8 35.929430430079115 37.0 35.0 37.0 35.0 37.0 9 37.639725434988634 39.0 37.0 39.0 35.0 39.0 10-11 37.558071656630744 39.0 37.0 39.0 35.0 39.0 12-13 37.4548159949104 39.0 37.0 39.0 35.0 39.0 14-15 38.679872418230545 40.0 38.0 41.0 34.5 41.0 16-17 38.67138427402871 40.0 38.0 41.0 34.5 41.0 18-19 38.77270898974325 40.0 38.0 41.0 35.0 41.0 20-21 38.76864598490734 40.0 38.0 41.0 34.5 41.0 22-23 38.675275741455664 40.0 38.0 41.0 34.0 41.0 24-25 38.5796584236698 40.0 38.0 41.0 34.0 41.0 26-27 38.386057754871075 40.0 38.0 41.0 34.0 41.0 28-29 38.230750019388324 40.0 38.0 41.0 34.0 41.0 30-31 38.2328866788386 40.0 38.0 41.0 34.0 41.0 32-33 38.111218677992085 40.0 37.5 41.0 33.5 41.0 34-35 37.99081650492201 40.0 37.0 41.0 33.0 41.0 36-37 37.85484846214412 40.0 37.0 41.0 33.0 41.0 38-39 37.68377440136073 40.0 37.0 41.0 33.0 41.0 40-41 37.32484781805731 40.0 36.5 41.0 32.0 41.0 42-43 37.14819780563563 40.0 36.0 41.0 31.0 41.0 44-45 36.946774768095885 39.0 36.0 41.0 31.0 41.0 46-47 36.60240257529572 39.0 36.0 41.0 30.5 41.0 48-49 36.24056774281745 39.0 35.0 40.0 30.0 41.0 50-51 35.95811003894754 39.0 35.0 40.0 29.0 41.0 52-53 35.69226780341418 39.0 35.0 40.0 28.5 41.0 54-55 35.32223400857293 38.0 35.0 40.0 28.0 41.0 56-57 35.10047491544717 38.0 34.0 40.0 27.0 41.0 58-59 34.76526387185564 38.0 34.0 40.0 26.5 41.0 60-61 34.376025773988616 37.0 34.0 40.0 26.0 41.0 62-63 33.97839154432303 37.0 33.0 39.5 26.0 40.5 64-65 33.65767639763554 36.0 33.0 39.0 25.0 40.0 66-67 33.32302860139833 36.0 32.5 39.0 24.5 40.0 68-69 32.86483115066768 35.5 32.0 38.5 23.0 40.0 70-71 32.4168378754599 35.0 31.5 38.0 22.0 40.0 72-73 32.00249616505448 35.0 31.0 37.0 21.5 39.5 74-75 31.522615334261346 35.0 31.0 37.0 20.0 39.0 76-77 30.087229729996174 33.5 29.5 35.0 18.5 37.5 78-79 30.764483738122184 34.0 30.5 36.0 19.0 38.0 80-81 30.645290213692235 34.0 30.5 35.5 18.0 37.0 82-83 30.302580159234033 34.0 30.0 35.0 17.0 37.0 84-85 29.86579465197623 34.0 30.0 35.0 11.5 36.0 86-87 29.494970864929734 34.0 29.0 35.0 7.0 36.0 88-89 29.110838798212853 34.0 29.0 35.0 4.5 35.5 90-91 28.677195514526787 33.5 29.0 35.0 2.0 35.0 92-93 28.17531188918552 33.0 28.5 35.0 2.0 35.0 94-95 27.6244717502205 33.0 27.0 34.5 2.0 35.0 96-97 26.863067798682117 32.5 25.5 34.0 2.0 35.0 98-99 26.017512589268463 32.0 24.5 34.0 2.0 35.0 100-101 22.86006950879836 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17897810459042063 1101 2 0.28889856188029484 1101 3 0.20454955118361084 1101 4 0.08180430223402624 1101 5 0.05895735153833925 1101 6 0.10757395559084415 1101 7 0.09418053507856428 1101 8 0.1664656126057409 1101 9 0.22633308631897364 1101 10-11 0.23128767345331624 1101 12-13 0.23388490528971317 1101 14-15 0.3604480595550612 1101 16-17 0.2843152576545833 1101 18-19 0.23238736875259747 1101 20-21 0.07594885016522568 1101 22-23 0.1508071701276137 1101 24-25 0.17192943154065432 1101 26-27 0.10063794418471161 1101 28-29 0.1851490777723015 1101 30-31 0.2558488027009531 1101 32-33 0.3623190060041708 1101 34-35 0.07216047829946604 1101 36-37 0.14064507829855444 1101 38-39 0.21494476576364718 1101 40-41 0.36447727842251965 1101 42-43 0.211104911098829 1101 44-45 0.21148441624245606 1101 46-47 -0.07604729464895144 1101 48-49 -0.056782792928324 1101 50-51 -0.157223194676412 1101 52-53 -0.13930377726929777 1101 54-55 -0.1894943944844627 1101 56-57 -0.1614551687927701 1101 58-59 0.010872471641718562 1101 60-61 -0.01749139246847875 1101 62-63 0.2104856196171241 1101 64-65 0.2748808261015725 1101 66-67 -0.022310304473350584 1101 68-69 -0.19193183344734166 1101 70-71 -0.25736361213034087 1101 72-73 -0.2824621502763769 1101 74-75 -0.30720567484926065 1101 76-77 -0.20313486195715313 1101 78-79 -0.16593999388440395 1101 80-81 -0.2668302372416562 1101 82-83 -0.11089489628248472 1101 84-85 -0.38680052287594435 1101 86-87 -0.38687819186797157 1101 88-89 -0.10642619795507713 1101 90-91 -0.029168382847011287 1101 92-93 -0.35842432504813004 1101 94-95 -0.6286890602442696 1101 96-97 -0.5539804870334883 1101 98-99 -0.35005891759171703 1101 100-101 -0.09047555789159745 1102 1 -0.08642574319328844 1102 2 -0.09168453838243806 1102 3 0.0616137091631046 1102 4 0.07002364285611407 1102 5 -3.697100770452266E-4 1102 6 0.09964955521792973 1102 7 0.02408984942724146 1102 8 0.12332636351683846 1102 9 0.09818663322973009 1102 10-11 0.11655337393640508 1102 12-13 0.18786405606520873 1102 14-15 0.16979630940674184 1102 16-17 0.21399150140405254 1102 18-19 0.21764035629391287 1102 20-21 0.22460551598436496 1102 22-23 0.0367297059357341 1102 24-25 0.12688324118554561 1102 26-27 0.2210930675002416 1102 28-29 0.1066890387859516 1102 30-31 0.2059293179988515 1102 32-33 0.09680921703900935 1102 34-35 0.24600995706884987 1102 36-37 0.1250929128608007 1102 38-39 0.2437819553559919 1102 40-41 0.3887589972122498 1102 42-43 0.2656010548398271 1102 44-45 0.08984275169461853 1102 46-47 0.15939638701898673 1102 48-49 0.04420034123558736 1102 50-51 -0.019859858800494123 1102 52-53 0.08219804491902494 1102 54-55 0.2519201321703335 1102 56-57 0.06381815945537994 1102 58-59 -0.016057776684412772 1102 60-61 -0.03306474614252153 1102 62-63 0.07630051918489045 1102 64-65 0.03200007349541778 1102 66-67 0.11954493749631467 1102 68-69 0.11078449844519156 1102 70-71 0.039822590090182075 1102 72-73 0.14409942273828236 1102 74-75 -0.06754553415936826 1102 76-77 0.08231305735804284 1102 78-79 -0.3897725220647601 1102 80-81 -0.022953637529816717 1102 82-83 0.17948988208856065 1102 84-85 0.12773460721982843 1102 86-87 -0.12304227356981201 1102 88-89 -0.21696522668632667 1102 90-91 -0.3377962326647932 1102 92-93 -0.17148051625077443 1102 94-95 -0.20043242832631236 1102 96-97 -0.023427050301563668 1102 98-99 -0.3412657995308628 1102 100-101 -0.13897543076218355 1103 1 0.08394720475190809 1103 2 0.2068888417813639 1103 3 0.10558781403241113 1103 4 0.10929638690157617 1103 5 0.0336610607399237 1103 6 0.029110150203592866 1103 7 0.09106066992357142 1103 8 0.11587174579153015 1103 9 0.05122864634279978 1103 10-11 0.12795812508512228 1103 12-13 0.11129380426328339 1103 14-15 0.12580592511746858 1103 16-17 0.31860678479483795 1103 18-19 0.24033997250658956 1103 20-21 0.15916089110967846 1103 22-23 0.19841419178416686 1103 24-25 0.17601385232853772 1103 26-27 0.11782354949316698 1103 28-29 0.015576855946356716 1103 30-31 0.1259512409510748 1103 32-33 0.0353086208052531 1103 34-35 -0.055512372190555936 1103 36-37 0.10175568603286678 1103 38-39 0.08697213832310524 1103 40-41 0.18742734065917688 1103 42-43 -0.012936429202952127 1103 44-45 0.013197546729941223 1103 46-47 -0.16554694025025896 1103 48-49 -0.08657766581068671 1103 50-51 -0.2942566045444366 1103 52-53 -0.3372079733112159 1103 54-55 -0.25072696934677197 1103 56-57 -0.3084290923750359 1103 58-59 -0.385211599567036 1103 60-61 -0.52446819493764 1103 62-63 -0.3450444457940094 1103 64-65 -0.46182086916439147 1103 66-67 -0.2645377918027094 1103 68-69 -0.36773446775737284 1103 70-71 -0.1894106356633145 1103 72-73 -0.06125053730930219 1103 74-75 -0.443054435610545 1103 76-77 -0.29978867002407483 1103 78-79 -0.30689942149999894 1103 80-81 -0.2325097927106725 1103 82-83 -0.03570743126449827 1103 84-85 -0.26708541700937616 1103 86-87 -0.31921471061254536 1103 88-89 -0.24250051436402842 1103 90-91 -0.4976574234772322 1103 92-93 -0.5214666787438063 1103 94-95 -0.003749224482195501 1103 96-97 0.14933897237838067 1103 98-99 -0.07498095309327368 1103 100-101 0.2303506826483961 1104 1 -0.07894299119517711 1104 2 0.019818069131339655 1104 3 0.05302294847820832 1104 4 0.08444252923351314 1104 5 -0.30093553317976784 1104 6 -0.09096487253251695 1104 7 0.13301449006308985 1104 8 -0.02706734890320206 1104 9 -0.07758786484233582 1104 10-11 0.039735131681389646 1104 12-13 0.14352154841994036 1104 14-15 0.09043334053439622 1104 16-17 0.03638140227421616 1104 18-19 0.23140182418381983 1104 20-21 0.16957441787388206 1104 22-23 0.13160479642311174 1104 24-25 0.10798799971352224 1104 26-27 0.15850165678358508 1104 28-29 0.13519071557242768 1104 30-31 0.1333916826371251 1104 32-33 -0.0565281051255937 1104 34-35 -0.015313891710121652 1104 36-37 -0.04393750567101051 1104 38-39 0.1300153167588718 1104 40-41 0.26570355245756616 1104 42-43 0.18080145667764214 1104 44-45 0.3274626013009083 1104 46-47 0.03767055268845354 1104 48-49 0.02249814442370024 1104 50-51 0.09090776961772207 1104 52-53 0.10014039892040927 1104 54-55 0.3329118738122574 1104 56-57 0.5072653960817206 1104 58-59 0.10034592258725894 1104 60-61 0.1592301614495497 1104 62-63 -0.006678251368676058 1104 64-65 0.34894595835115894 1104 66-67 -0.058752127019339184 1104 68-69 -0.007332920928710962 1104 70-71 -0.011360024514843303 1104 72-73 0.12042497046391176 1104 74-75 -0.08596433189607566 1104 76-77 0.32538061634103954 1104 78-79 0.5706420209185907 1104 80-81 0.46310022646581217 1104 82-83 0.69393462200199 1104 84-85 0.5783321932382357 1104 86-87 0.5665306260150551 1104 88-89 0.1786049545838928 1104 90-91 0.32793231998039474 1104 92-93 0.2931629265538156 1104 94-95 0.25148738139166227 1104 96-97 0.34954344712047103 1104 98-99 0.25144867401727566 1104 100-101 0.7294485376817299 1105 1 0.010802578672716834 1105 2 0.06702819279406214 1105 3 0.10965450358070683 1105 4 0.03366976592252513 1105 5 0.05306316944278677 1105 6 0.18116519470458314 1105 7 0.19849086440796526 1105 8 0.09453604487144673 1105 9 0.03328642340198229 1105 10-11 0.08321272103054156 1105 12-13 0.058234426435575415 1105 14-15 -0.0551097560790339 1105 16-17 -0.10361695026237072 1105 18-19 0.16881880747236266 1105 20-21 0.31524622256709733 1105 22-23 0.19924861483145406 1105 24-25 0.10482692927416082 1105 26-27 -0.05162200593946409 1105 28-29 -0.06443036784070699 1105 30-31 0.12695113032501837 1105 32-33 0.049244194836305155 1105 34-35 -0.0853104938168201 1105 36-37 -0.12882305844646424 1105 38-39 -0.041449587121931586 1105 40-41 0.020730890053705764 1105 42-43 -0.11867650335077684 1105 44-45 -0.15859573949749972 1105 46-47 -0.16712974385497148 1105 48-49 -0.46446115293314705 1105 50-51 -0.18968614094561786 1105 52-53 0.12465156563340685 1105 54-55 0.3146610680996744 1105 56-57 0.05527865816225841 1105 58-59 -0.21055101827910505 1105 60-61 -0.15170281767914418 1105 62-63 -0.2340127585196896 1105 64-65 -0.06858305245002327 1105 66-67 -0.11459598867359944 1105 68-69 -0.11532929650918788 1105 70-71 -0.20628583717765991 1105 72-73 -0.08434443614035558 1105 74-75 0.035721435049627104 1105 76-77 -0.08425601500813329 1105 78-79 0.21123757894534023 1105 80-81 0.14678724960237588 1105 82-83 0.10071908230327153 1105 84-85 -0.23348629747933813 1105 86-87 -0.12475017382843845 1105 88-89 0.18620902793720262 1105 90-91 0.06055930337189963 1105 92-93 0.15110524474078346 1105 94-95 0.15927576471521476 1105 96-97 0.22843944069536448 1105 98-99 0.21698579857580924 1105 100-101 0.3732360560869843 1106 1 -0.013481480557580028 1106 2 0.139803436268231 1106 3 0.15186876084872836 1106 4 0.04175238422019589 1106 5 -0.03222519810364588 1106 6 0.07776108189186459 1106 7 0.07297042705957324 1106 8 0.21663969788907878 1106 9 0.22242693243496348 1106 10-11 0.2486439146875412 1106 12-13 0.32137660086807784 1106 14-15 0.2389712873299672 1106 16-17 0.11062674113185977 1106 18-19 0.13811526668817464 1106 20-21 -0.019664262208983985 1106 22-23 0.1648431805604318 1106 24-25 0.13753528523520941 1106 26-27 0.40386928691741275 1106 28-29 0.30234445928358156 1106 30-31 0.3514542257958553 1106 32-33 0.05448024711536448 1106 34-35 -0.1129389287033149 1106 36-37 0.08012122307845715 1106 38-39 0.18392467521469769 1106 40-41 0.3644096127646961 1106 42-43 0.2663769885575533 1106 44-45 -0.08461896457986029 1106 46-47 -0.14403590221273532 1106 48-49 -0.22116574364311958 1106 50-51 -0.10150821473005323 1106 52-53 -0.3543929113949318 1106 54-55 -0.239093321676215 1106 56-57 -0.2330300562217218 1106 58-59 -0.09139801874976428 1106 60-61 -0.3835010871118172 1106 62-63 -0.2619806090681962 1106 64-65 -0.4115580533551295 1106 66-67 -0.44012728732317896 1106 68-69 -0.53122435823758 1106 70-71 -0.6128220777192794 1106 72-73 -0.29653353139396543 1106 74-75 -0.15395987627146823 1106 76-77 -0.1891988121552295 1106 78-79 -0.34213213974695833 1106 80-81 -0.19617191190517502 1106 82-83 -0.12162759232835185 1106 84-85 0.07743202553576012 1106 86-87 0.1589560641788772 1106 88-89 0.13029595455101273 1106 90-91 -0.2165622707926822 1106 92-93 0.009435410194605254 1106 94-95 0.08190030763545764 1106 96-97 0.1381684512308219 1106 98-99 0.13098239536810397 1106 100-101 0.6072065857564404 1107 1 0.3066148349904978 1107 2 0.3273193614580592 1107 3 0.1706222643904951 1107 4 0.0974141077079409 1107 5 0.05493018078847456 1107 6 0.024373475511602294 1107 7 0.11781576161595808 1107 8 0.28406695162193785 1107 9 0.22875878791067805 1107 10-11 0.14024433256452085 1107 12-13 0.09571029092565908 1107 14-15 0.05579656888732387 1107 16-17 0.20891064267596704 1107 18-19 0.24397000347664743 1107 20-21 0.2781081222378461 1107 22-23 0.19681777669761402 1107 24-25 0.23707090669981312 1107 26-27 0.15378647593031047 1107 28-29 0.26873275363205096 1107 30-31 0.1811183953927582 1107 32-33 0.10261891092824271 1107 34-35 0.15885310105768013 1107 36-37 -0.0185257727937298 1107 38-39 0.0014251848207749163 1107 40-41 0.20733628045056918 1107 42-43 0.1814415811274941 1107 44-45 -0.1088220680808547 1107 46-47 -0.32553847572341965 1107 48-49 -0.3217970181679064 1107 50-51 -0.5440087243361305 1107 52-53 0.008322397171546925 1107 54-55 -0.0041776614815489665 1107 56-57 -0.03799744143295669 1107 58-59 0.05073748550545787 1107 60-61 0.2149548064824316 1107 62-63 0.13484964114839215 1107 64-65 0.19127726744189744 1107 66-67 0.2336229037790929 1107 68-69 0.13652840637537622 1107 70-71 0.09065407271643267 1107 72-73 0.16275531332906112 1107 74-75 0.06147927676323661 1107 76-77 -0.12535154109575686 1107 78-79 0.07871966680657394 1107 80-81 0.17727378956758955 1107 82-83 0.2412023776048926 1107 84-85 -0.18624452748875342 1107 86-87 0.1264559367773579 1107 88-89 0.2642777616141494 1107 90-91 0.3786639943044001 1107 92-93 0.4465850560183817 1107 94-95 0.9369205644769174 1107 96-97 0.9974725841360872 1107 98-99 0.5232261909141442 1107 100-101 1.080070062289085 1108 1 0.12260811572011932 1108 2 0.13731031628638846 1108 3 0.09820461921965773 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-0.7801079285086701 2316 64-65 -1.0199048105564472 2316 66-67 -1.1865623158021634 2316 68-69 -1.3607913620302483 2316 70-71 -1.2331466634485153 2316 72-73 -1.2300514004313925 2316 74-75 -1.127189454999904 2316 76-77 -1.1631344183703085 2316 78-79 -0.9421909328902522 2316 80-81 -1.199684207488552 2316 82-83 -1.1426359457125272 2316 84-85 -0.9866032231626356 2316 86-87 -0.8665628787679864 2316 88-89 -1.6344686010000125 2316 90-91 -1.583552129825076 2316 92-93 -1.5519549966675434 2316 94-95 -1.5944952648670245 2316 96-97 -1.3129329856328162 2316 98-99 -1.6314593212557433 2316 100-101 -2.305222216766566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 10.0 6 25.0 7 93.0 8 228.0 9 416.0 10 679.0 11 1034.0 12 1588.0 13 2253.0 14 2830.0 15 3982.0 16 5115.0 17 7089.0 18 6717.0 19 4512.0 20 4003.0 21 4166.0 22 4393.0 23 4829.0 24 5498.0 25 6505.0 26 8126.0 27 9859.0 28 12107.0 29 15160.0 30 18920.0 31 23331.0 32 30026.0 33 39778.0 34 54297.0 35 78695.0 36 120874.0 37 171795.0 38 106216.0 39 5611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.578305776431485 19.827427531658884 15.234785855526106 22.359480836383526 2 15.780867151104708 20.93871262868981 39.75174303050697 23.528677189698506 3 19.507257845084474 24.842427930906208 30.043784989543393 25.606529234465924 4 13.415276950761534 17.82963772087906 36.68350493646543 32.071580391893974 5 13.930594807755503 37.381268485047656 34.06585606309563 14.622280644101215 6 31.628343500703892 36.80036068862083 17.135864200208474 14.435431610466805 7 27.721138957314075 33.41001043684918 20.76499212635686 18.103858479479893 8 26.042796283224696 35.34669616321423 19.455365440404222 19.155142113156852 9 26.13835773633714 17.14519688682606 20.16767288801959 36.54877248881721 10-11 24.728349631588667 27.25869238458844 27.090506745499443 20.922451238323454 12-13 25.81025745515732 24.563864259282386 27.633805038680713 21.99207324687958 14-15 22.846371298227304 25.887186511439285 26.0626750366587 25.20376715367471 16-17 22.0252061406449 28.969053599853833 26.60748954673376 22.39825071276751 18-19 21.886614626567265 27.4340971095333 29.344424880662025 21.33486338323741 20-21 22.825909073880677 26.439529917759923 29.501071625038367 21.233489383321032 22-23 22.42366086099244 26.486953664147222 29.349260598093984 21.740124876766348 24-25 22.247555076502444 26.486999842263 29.60664335664336 21.658801724591196 26-27 21.90568453374625 26.66910718933286 29.513937268489915 21.911271008430976 28-29 21.798555666262686 26.964007329454788 29.697305084612086 21.54013191967044 30-31 22.153234827483317 26.710740607833937 29.33638025834454 21.79964430633821 32-33 22.152292657855803 26.665509937030528 29.3696283545981 21.812569050515567 34-35 22.228224936149964 26.906858473093603 29.4959560548762 21.368960535880234 36-37 22.50601212349056 26.623897722589877 29.278959243750773 21.591130910168786 38-39 22.26801331929365 26.965785505737472 29.186697532933437 21.579503642035437 40-41 22.3314913205444 26.84906711954593 29.15267849866318 21.66676306124649 42-43 22.297234238603934 27.00800639354756 28.894063491187183 21.800695876661315 44-45 22.33934179065436 27.070854052718264 28.748056222650742 21.841747933976634 46-47 22.531121613690424 27.397798664634504 28.34053331808553 21.730546403589543 48-49 22.44506034374542 27.55096340335701 28.144115178573188 21.85986107432438 50-51 22.54886625041078 27.693328951692408 28.0302990469931 21.727505750903713 52-53 22.686076102444385 27.82328028140133 27.744083579138866 21.74656003701542 54-55 22.546850744051728 27.828888477023845 27.702568591960482 21.921692186963945 56-57 22.449537570126875 28.007502983842556 27.563147046915994 21.97981239911457 58-59 22.381857173747164 27.655506606192333 27.89605442427246 22.06658179578805 60-61 22.48991443868544 27.755438447513676 27.860664799189212 21.893982314611673 62-63 22.245327574229414 27.719740551141513 28.084109907573477 21.950821967055596 64-65 22.20597397885815 27.820183867312682 28.04430008701756 21.929542066811607 66-67 22.17466057281664 28.05346446415263 27.77584989885208 21.99602506417865 68-69 22.303507254530302 27.854425670968467 27.82807065510288 22.013996419398342 70-71 22.30727572187396 27.936952769785073 27.7823711195208 21.97340038882017 72-73 22.27071882391834 27.945807074077557 27.507628849175862 22.275845252828244 74-75 22.271005260681076 28.11776196272567 27.405887440879777 22.205345335713478 76-77 22.209961164065884 28.187500862619114 27.453239471610463 22.149298501704536 78-79 22.347848775803815 28.245666037255944 27.1321808522874 22.27430433465284 80-81 22.355687706540685 28.11115668041633 27.200800267106235 22.33235534593675 82-83 22.285433365844426 28.348467202792975 27.307871277107548 22.05822815425505 84-85 22.287793823431212 28.466597261109488 26.97687533026792 22.268733585191377 86-87 22.187447215856178 28.0721275402934 27.152787955783893 22.587637288066528 88-89 22.142519597578424 28.14427182845226 27.238514376377353 22.474694197591962 90-91 22.039982832764707 28.155843268136838 27.228470794996788 22.57570310410167 92-93 22.08692017398231 28.11412692716693 27.243032360762232 22.555920538088532 94-95 21.859189475884204 28.275345802328438 27.25755717585016 22.60790754593719 96-97 21.981272760851432 28.22953519720649 27.202343435246302 22.58684860669577 98-99 21.65245498245831 28.400189810439315 26.91627703385698 23.031078173245405 100-101 21.454678338550803 28.842760160921095 26.786684903164065 22.915876597364033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 252.0 1 186.5 2 144.0 3 176.5 4 161.0 5 155.5 6 259.0 7 528.5 8 888.5 9 1121.0 10 1091.0 11 951.5 12 947.0 13 1033.5 14 1154.0 15 1186.0 16 1217.0 17 1300.0 18 1370.5 19 1471.5 20 1587.5 21 1822.0 22 2109.0 23 2548.0 24 3119.0 25 3841.5 26 4934.5 27 6414.5 28 7547.0 29 8481.5 30 9788.5 31 11247.5 32 12691.0 33 14013.5 34 15512.5 35 16758.5 36 18311.0 37 20024.5 38 21061.0 39 21675.5 40 22239.5 41 23196.0 42 23625.0 43 24117.0 44 25192.0 45 25809.5 46 26249.5 47 27181.5 48 28417.5 49 28510.5 50 27466.0 51 26684.5 52 26590.5 53 26289.5 54 25694.0 55 24250.5 56 21534.5 57 19216.5 58 17890.0 59 16052.0 60 13272.5 61 10766.5 62 8459.0 63 6196.5 64 4314.0 65 3032.5 66 2273.5 67 1855.0 68 1526.0 69 1295.5 70 977.5 71 600.0 72 408.5 73 250.0 74 154.0 75 99.0 76 52.0 77 25.0 78 16.0 79 10.5 80 7.0 81 4.5 82 4.5 83 2.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02089996017177401 2 0.0030232646789358636 3 5.257851615540632E-4 4 0.0 5 0.0022345869366047683 6 0.0 7 1.314462903885158E-4 8 0.0 9 0.0 10-11 6.57231451942579E-5 12-13 0.006835207100202822 14-15 0.004403450728015279 16-17 0.0 18-19 9.858471779138685E-4 20-21 8.544008875253527E-4 22-23 0.0022345869366047683 24-25 9.201240327196106E-4 26-27 1.314462903885158E-4 28-29 1.314462903885158E-4 30-31 0.0 32-33 1.9716943558277373E-4 34-35 0.0 36-37 0.0016430786298564475 38-39 0.008675455165642044 40-41 6.57231451942579E-4 42-43 0.0 44-45 7.886777423310949E-4 46-47 1.9716943558277373E-4 48-49 6.57231451942579E-5 50-51 0.0022345869366047683 52-53 3.9433887116554746E-4 54-55 0.0 56-57 3.9433887116554746E-4 58-59 4.600620163598053E-4 60-61 0.003417603550101411 62-63 6.57231451942579E-5 64-65 1.314462903885158E-4 66-67 0.0 68-69 1.314462903885158E-4 70-71 5.257851615540632E-4 72-73 4.600620163598053E-4 74-75 0.0038119424212669584 76-77 3.286157259712895E-4 78-79 3.286157259712895E-4 80-81 0.002760372098158832 82-83 1.314462903885158E-4 84-85 0.002760372098158832 86-87 4.600620163598053E-4 88-89 0.0029575415337416053 90-91 0.002037417501021995 92-93 0.0 94-95 0.0 96-97 4.600620163598053E-4 98-99 0.0010515703231081263 100-101 0.0028260952433530897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 760767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.99528638032267 #Duplication Level Percentage of deduplicated Percentage of total 1 83.52036259452048 56.7899097320281 2 10.072806872385495 13.698067758830684 3 2.755485410837818 5.620800588801556 4 1.1922167667217456 3.242604819226698 5 0.6705696627567818 2.279778812855189 6 0.3939227878428703 1.6070935662666634 7 0.2641557904120601 1.2572944032661966 8 0.1925633807879168 1.0474721778430023 9 0.14397148542817972 0.8810444144060582 >10 0.7383406741412167 8.848763048462228 >50 0.04102339911519177 1.887125749071827 >100 0.013803512383278255 1.6751559005570873 >500 1.9441566675222751E-4 0.1130254334168142 >1k 5.832470002566825E-4 1.0518635949679422 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3378 0.44402556893240636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2772 0.3643691169569658 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1807 0.23752344673204803 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.11238657828218102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.57231451942579E-5 0.0 18-19 0.0 0.0 0.0 1.314462903885158E-4 0.0 20-21 0.0 0.0 0.0 2.628925807770316E-4 0.0 22-23 0.0 0.0 0.0 9.858471779138685E-4 0.0 24-25 0.0 0.0 0.0 0.001314462903885158 0.0 26-27 0.0 0.0 0.0 0.0017088017750507053 0.0 28-29 0.0 0.0 0.0 0.002628925807770316 0.0 30-31 0.0 0.0 0.0 0.006177975648260243 0.0 32-33 0.0 0.0 0.0 0.010449980085887007 0.0 34-35 0.0 0.0 0.0 0.013999029926376933 0.0 36-37 0.0 0.0 0.0 0.017088017750507056 0.0 38-39 1.314462903885158E-4 0.0 0.0 0.022740208237213233 0.0 40-41 1.314462903885158E-4 0.0 0.0 0.027143658965228513 0.0 42-43 1.314462903885158E-4 0.0 0.0 0.04028828800408009 0.0 44-45 1.314462903885158E-4 0.0 0.0 0.05586467341511921 0.0 46-47 1.314462903885158E-4 0.0 0.0 0.06815490156644544 0.0 48-49 1.314462903885158E-4 0.0 0.0 0.08149670004087979 0.0 50-51 1.314462903885158E-4 0.0 0.0 0.09858471779138685 0.0 52-53 1.314462903885158E-4 0.0 0.0 0.13387804676070333 0.0 54-55 1.314462903885158E-4 0.0 0.0 0.1640449704048677 0.0 56-57 2.628925807770316E-4 0.0 0.0 0.22181561503062042 0.0 58-59 2.628925807770316E-4 0.0 0.0 0.30291797620033467 0.0 60-61 2.628925807770316E-4 0.0 0.0 0.37475337389765856 0.0 62-63 2.628925807770316E-4 0.0 0.0 0.47741292669108937 0.0 64-65 2.628925807770316E-4 0.0 0.0 0.543070348740153 0.0 66-67 2.628925807770316E-4 0.0 0.0 0.6728735604988123 0.0 68-69 2.628925807770316E-4 0.0 0.0 0.8356040679997949 0.0 70-71 2.628925807770316E-4 0.0 0.0 0.9310340748218574 0.0 72-73 2.628925807770316E-4 0.0 0.0 1.118476484915881 0.0 74-75 2.628925807770316E-4 0.0 0.0 1.3005295971039752 0.0 76-77 2.628925807770316E-4 0.0 0.0 1.4076583237706157 0.0 78-79 2.628925807770316E-4 0.0 0.0 1.5194533937460484 0.0 80-81 5.257851615540632E-4 0.0 0.0 1.736208326596711 0.0 82-83 5.257851615540632E-4 0.0 0.0 1.9201674099954387 0.0 84-85 5.257851615540632E-4 0.0 0.0 2.1994250539258404 0.0 86-87 5.257851615540632E-4 0.0 0.0 2.644502193181355 0.0 88-89 5.257851615540632E-4 0.0 0.0 3.2447516782405126 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1915 0.0 43.659306 1 GTATCAA 3420 0.0 38.336807 1 GACCGGT 50 0.0016157964 37.99845 7 CGCAGTA 55 0.002577933 34.548588 9 TAGACCG 90 2.412571E-5 31.665377 5 TATCAAC 4205 0.0 30.837029 2 ATCAACG 4290 0.0 30.226038 3 TCAACGC 4360 0.0 29.849699 4 CAACGCA 4400 0.0 29.578337 5 CTATGCG 65 0.005840258 29.23342 9 AACGCAG 4505 0.0 28.888945 6 TCGTACC 90 8.9400203E-4 26.391281 9 ACGCAGA 5060 0.0 25.626423 7 CGCAGAG 5175 0.0 25.060244 8 GTAAGGT 330 0.0 23.029362 4 GCAGAGT 5550 0.0 23.02461 9 GAGTACT 3270 0.0 22.154877 12-13 AGTACTT 3345 0.0 21.942173 12-13 CGATCCG 55 0.0028773735 21.591446 70-71 AGAGTAC 4770 0.0 21.511385 10-11 >>END_MODULE