FastQCFastQC Report
Thu 26 May 2016
SRR937019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206651
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115320.5226018976267656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85530.38760093916074634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54060.2449866335909031No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.10015176844911135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122450.035.0453031
GGTATCA76050.034.306311
ATCAACG147100.029.106723
TCAACGC148200.028.9227434
CAACGCA149050.028.8209115
TATCAAC149700.028.572692
AACGCAG151700.028.3481316
GTACATG99900.026.6869051
ACGCAGA170000.025.044977
TACATGG103250.025.0402852
CGCAGAG171050.024.8645788
ACATGGG101850.024.4951113
GCAGAGT176450.023.0533079
GAGTACT86400.022.57441512-13
CATGGGG46200.020.9830864
AGAGTAC144000.020.83901610-11
GTACTTT93600.020.7112514-15
AGTACTT89850.020.6512-13
CATGGGA60150.019.592834
GTGTAGC11700.019.0903031
Produced by FastQC (version 0.11.5)