##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937019_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2206651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.669859438579095 33.0 31.0 34.0 30.0 34.0 2 31.76974247400246 33.0 31.0 34.0 30.0 34.0 3 31.770850941086742 33.0 31.0 34.0 30.0 34.0 4 35.344639909074886 37.0 35.0 37.0 33.0 37.0 5 35.369797489498794 37.0 35.0 37.0 33.0 37.0 6 35.425156945978316 37.0 35.0 37.0 33.0 37.0 7 35.36372131342927 37.0 35.0 37.0 33.0 37.0 8 35.330730142646026 37.0 35.0 37.0 33.0 37.0 9 36.93429228273977 39.0 37.0 39.0 33.0 39.0 10-11 36.79778927433473 39.0 37.0 39.0 33.0 39.0 12-13 36.687698915687164 39.0 37.0 39.0 32.0 39.0 14-15 37.814409709555335 40.0 37.5 41.0 32.5 41.0 16-17 37.74637380356023 40.0 37.0 41.0 32.0 41.0 18-19 37.712547883648114 40.0 37.5 41.0 32.0 41.0 20-21 37.662333780919596 40.0 37.0 41.0 32.0 41.0 22-23 37.52251624747184 40.0 37.0 41.0 32.0 41.0 24-25 37.353918222682246 40.0 37.0 41.0 31.5 41.0 26-27 37.30662188991372 40.0 37.0 41.0 31.5 41.0 28-29 37.37308912918264 40.0 37.0 41.0 32.0 41.0 30-31 37.22778794653074 40.0 37.0 41.0 31.0 41.0 32-33 37.034027809563 39.5 36.0 41.0 31.0 41.0 34-35 36.835993095419255 39.0 36.0 41.0 30.0 41.0 36-37 36.67977944858521 39.0 36.0 41.0 30.0 41.0 38-39 36.52210725665273 39.0 35.0 41.0 30.0 41.0 40-41 36.30903618197893 39.0 35.0 41.0 30.0 41.0 42-43 36.096079987274834 39.0 35.0 40.5 29.5 41.0 44-45 35.839497727551844 39.0 35.0 40.0 28.5 41.0 46-47 35.47938459683928 39.0 35.0 40.0 27.0 41.0 48-49 35.12577249415517 38.0 34.5 40.0 25.5 41.0 50-51 34.47023996998166 37.5 33.5 39.5 24.0 40.5 52-53 34.78100637572503 38.0 34.5 40.0 26.0 40.5 54-55 35.08713407784013 39.0 35.0 40.0 26.0 41.0 56-57 34.979245925159894 39.0 35.0 40.5 25.5 41.0 58-59 34.7393314574892 38.0 34.0 40.0 25.0 41.0 60-61 34.454832458780295 38.0 34.0 40.0 24.0 41.0 62-63 34.15170273867503 38.0 34.0 40.0 23.0 41.0 64-65 33.84088512410889 37.0 33.5 40.0 22.5 41.0 66-67 33.47429498366529 36.5 33.0 39.5 22.0 41.0 68-69 33.034672451602 36.0 33.0 39.0 21.0 41.0 70-71 32.67608221689791 36.0 32.0 39.0 20.0 40.5 72-73 32.29657861619259 35.0 32.0 38.5 19.5 40.0 74-75 31.86347229353441 35.0 31.5 37.5 18.0 39.5 76-77 31.4229746797296 35.0 31.0 37.0 15.5 39.0 78-79 30.989656044385814 35.0 31.0 36.5 12.0 39.0 80-81 30.554568438778947 35.0 31.0 36.0 7.5 37.5 82-83 30.148979154383724 34.0 30.0 35.5 7.0 37.0 84-85 29.798360501955226 34.0 30.0 35.0 2.0 37.0 86-87 29.470620863924562 34.0 30.0 35.0 2.0 36.0 88-89 29.10096975008735 34.0 29.0 35.0 2.0 36.0 90-91 28.764671894196226 34.0 29.0 35.0 2.0 36.0 92-93 28.40880479060803 34.0 29.0 35.0 2.0 35.0 94-95 28.014539680266612 34.0 29.0 35.0 2.0 35.0 96-97 27.580379724750312 34.0 27.5 35.0 2.0 35.0 98-99 26.981903799014887 34.0 26.5 35.0 2.0 35.0 100-101 24.855067475554584 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3819722667890524 1101 2 0.24413683955404153 1101 3 0.33813112405972845 1101 4 0.31717825968664926 1101 5 0.26024270661949345 1101 6 0.13464610788601306 1101 7 0.269176494762398 1101 8 0.14147569954047157 1101 9 0.2405130639517239 1101 10-11 0.1946932833921622 1101 12-13 0.3927738097547291 1101 14-15 0.5418392111501831 1101 16-17 0.5996531919679668 1101 18-19 0.5401877634995955 1101 20-21 0.41495895382949755 1101 22-23 0.48924317993951405 1101 24-25 0.6306576146552629 1101 26-27 0.7170335707266204 1101 28-29 0.6623996140595523 1101 30-31 0.4811739918587037 1101 32-33 0.4550178304062982 1101 34-35 0.4601353598028908 1101 36-37 0.510414077709548 1101 38-39 0.5645969647643554 1101 40-41 0.5809309995419767 1101 42-43 0.6323341944994425 1101 44-45 0.6975843359840681 1101 46-47 0.6298479556354053 1101 48-49 0.6045686950942439 1101 50-51 0.7507992365520906 1101 52-53 0.7012934726097981 1101 54-55 0.7184083344108032 1101 56-57 0.6186216633587236 1101 58-59 0.6968540650614372 1101 60-61 0.6879492493524992 1101 62-63 0.7267655565711806 1101 64-65 0.7165956555956754 1101 66-67 0.5877482168686683 1101 68-69 0.6796149418147266 1101 70-71 0.7165605905602206 1101 72-73 0.7874029843552037 1101 74-75 0.7307968168385663 1101 76-77 0.6925098162819339 1101 78-79 0.798177439614399 1101 80-81 0.8360649969758462 1101 82-83 0.7116389157627978 1101 84-85 0.6997261728211477 1101 86-87 0.7921984598470111 1101 88-89 0.7816409638745405 1101 90-91 0.8671805304030293 1101 92-93 0.7647780270029365 1101 94-95 0.8321655596244462 1101 96-97 0.8247632072539766 1101 98-99 0.8820916398961707 1101 100-101 1.1494131129650036 1102 1 0.3508713573822355 1102 2 0.41568606185795076 1102 3 0.3568005581094127 1102 4 0.23695961121284626 1102 5 0.19676294310781373 1102 6 0.23748559100316413 1102 7 0.26278803203894086 1102 8 0.2952955470034553 1102 9 0.2767801227168505 1102 10-11 0.3758173987869924 1102 12-13 0.36120405271235256 1102 14-15 0.5123646120543199 1102 16-17 0.4235290411140724 1102 18-19 0.28607565454412764 1102 20-21 0.4339104056418108 1102 22-23 0.44698046609180153 1102 24-25 0.44695846196931655 1102 26-27 0.5794238741695352 1102 28-29 0.5516984622703305 1102 30-31 0.5367848372894812 1102 32-33 0.6458847572636941 1102 34-35 0.4736144535322282 1102 36-37 0.3611687648069406 1102 38-39 0.3506604490981502 1102 40-41 0.5285336909637977 1102 42-43 0.5372726966612689 1102 44-45 0.6336560547007011 1102 46-47 0.5409004673585684 1102 48-49 0.4754094452819757 1102 50-51 0.298017315316919 1102 52-53 0.4526144015742659 1102 54-55 0.4944621975183239 1102 56-57 0.5451653737479987 1102 58-59 0.4144525565540178 1102 60-61 0.49728664550669066 1102 62-63 0.5585079598605205 1102 64-65 0.4746472666585859 1102 66-67 0.37905255099077095 1102 68-69 0.38145313973776496 1102 70-71 0.5050071604472777 1102 72-73 0.5823730766039361 1102 74-75 0.6755828765405987 1102 76-77 0.5918724198913523 1102 78-79 0.6205098738634405 1102 80-81 0.551035262580001 1102 82-83 0.5931753806831459 1102 84-85 0.6826760360673525 1102 86-87 0.6823136545291 1102 88-89 0.7116065528220261 1102 90-91 0.6612249046647918 1102 92-93 0.5453920046511165 1102 94-95 0.6387817483423213 1102 96-97 0.5998613303662736 1102 98-99 0.7560570428805633 1102 100-101 1.1426902318608292 1103 1 0.2887301557337274 1103 2 0.33249706133515744 1103 3 0.33390068184981203 1103 4 0.23000854925494707 1103 5 0.17004254713853584 1103 6 0.12589672770113935 1103 7 0.20486463332741067 1103 8 0.15592846690812223 1103 9 0.3550350019095916 1103 10-11 0.3269730994139408 1103 12-13 0.06830296476989872 1103 14-15 0.30008931956925977 1103 16-17 0.4026715167339958 1103 18-19 0.4749332390435157 1103 20-21 0.2673105984574349 1103 22-23 0.2786519190606853 1103 24-25 0.6303234973885736 1103 26-27 0.4945874033213542 1103 28-29 0.5143905612614148 1103 30-31 0.5948939395688413 1103 32-33 0.4922765299681018 1103 34-35 0.3673301115426497 1103 36-37 0.47276382597522826 1103 38-39 0.3970462098722862 1103 40-41 0.512638798098564 1103 42-43 0.5051290127673198 1103 44-45 0.5345411719813811 1103 46-47 0.3961045987322578 1103 48-49 0.3355910830495432 1103 50-51 0.24472232818213513 1103 52-53 0.3357518513444262 1103 54-55 0.3401737544006309 1103 56-57 0.4736011686568844 1103 58-59 0.43168316707173915 1103 60-61 0.36930210174556777 1103 62-63 0.5017069947287922 1103 64-65 0.42639145468697137 1103 66-67 0.46581779781924837 1103 68-69 0.5662560801294845 1103 70-71 0.41243040684623367 1103 72-73 0.45970606967441796 1103 74-75 0.4005287769154755 1103 76-77 0.371602344770114 1103 78-79 0.44199211217325995 1103 80-81 0.5527093062569861 1103 82-83 0.467307873089986 1103 84-85 0.6404634535931244 1103 86-87 0.5767815010835662 1103 88-89 0.5372379526999929 1103 90-91 0.46782459408971633 1103 92-93 0.44690419105070234 1103 94-95 0.4056108145996795 1103 96-97 0.2739725640918387 1103 98-99 0.30936062955477794 1103 100-101 0.6217372603403319 1104 1 0.39932596047277613 1104 2 0.3126933803946521 1104 3 0.3356398617832781 1104 4 0.34300786449888676 1104 5 0.3150981563621471 1104 6 0.2160421002402444 1104 7 0.2585371190253909 1104 8 0.3055150182321782 1104 9 0.4369626320676545 1104 10-11 0.3401051803189574 1104 12-13 0.4551447660299175 1104 14-15 0.5199759581201917 1104 16-17 0.43664579736343256 1104 18-19 0.48785763293395235 1104 20-21 0.46845936464085725 1104 22-23 0.5652168880122801 1104 24-25 0.5008467581667801 1104 26-27 0.5434825854179124 1104 28-29 0.5284499927297119 1104 30-31 0.5405364466801004 1104 32-33 0.4137808837777115 1104 34-35 0.5518165361282357 1104 36-37 0.5156745917133136 1104 38-39 0.5151927476686424 1104 40-41 0.6327358024163772 1104 42-43 0.5956761981520486 1104 44-45 0.661630891767139 1104 46-47 0.6215295576720123 1104 48-49 0.6489146110332982 1104 50-51 0.6133464372877171 1104 52-53 0.7360425796804435 1104 54-55 0.7383707866779261 1104 56-57 0.8049942203082523 1104 58-59 0.8100136514403786 1104 60-61 0.830535700666033 1104 62-63 0.6857861652531341 1104 64-65 0.6505593253969906 1104 66-67 0.6222797854869384 1104 68-69 0.7364244062273926 1104 70-71 0.7215324723316883 1104 72-73 0.6855979686251814 1104 74-75 0.717566562390946 1104 76-77 0.8541515291737483 1104 78-79 0.8957121457246302 1104 80-81 0.9660116553553344 1104 82-83 0.9940280924797982 1104 84-85 0.9478604932369841 1104 86-87 0.8816569976991282 1104 88-89 0.8426381499231326 1104 90-91 0.9185021510006415 1104 92-93 0.8440054462135791 1104 94-95 0.9038638495423292 1104 96-97 0.9040425632379865 1104 98-99 0.8611790001032382 1104 100-101 1.1989382320577313 1105 1 0.37547678144180807 1105 2 0.3906557721396986 1105 3 0.32369482886464596 1105 4 0.3443765265684817 1105 5 0.26762134773902346 1105 6 0.3953567993323617 1105 7 0.36698167081090816 1105 8 0.31759659406674245 1105 9 0.4497731340815676 1105 10-11 0.5325193465514033 1105 12-13 0.4124829969053536 1105 14-15 0.38782837386484204 1105 16-17 0.5754804663324364 1105 18-19 0.5481160375497822 1105 20-21 0.6243441170950206 1105 22-23 0.5626158215322263 1105 24-25 0.5816394677639636 1105 26-27 0.7115815581131315 1105 28-29 0.6356810621383957 1105 30-31 0.4435118651517982 1105 32-33 0.5365532184333688 1105 34-35 0.5062866050843127 1105 36-37 0.5425287608363618 1105 38-39 0.5519615869777894 1105 40-41 0.77815467348411 1105 42-43 0.6813995713325838 1105 44-45 0.5564068891199625 1105 46-47 0.5941306431691729 1105 48-49 0.6480573192992765 1105 50-51 0.5864725854462876 1105 52-53 0.43188011262759574 1105 54-55 0.41877107802019253 1105 56-57 0.6012120005540282 1105 58-59 0.5208015877406424 1105 60-61 0.49645169824705704 1105 62-63 0.37400467955971806 1105 64-65 0.5624333293753736 1105 66-67 0.6224840293472624 1105 68-69 0.7652868782246927 1105 70-71 0.7424579594551943 1105 72-73 0.6690576390204725 1105 74-75 0.706354924674681 1105 76-77 0.6613621551792761 1105 78-79 0.5950877186153463 1105 80-81 0.6681695278721733 1105 82-83 0.6718054476549256 1105 84-85 0.7426103420508845 1105 86-87 0.7429618192995342 1105 88-89 0.7748005675314644 1105 90-91 0.7921444237745945 1105 92-93 0.8225808547963886 1105 94-95 0.8912593980150625 1105 96-97 1.1267955955176419 1105 98-99 1.2444989897579255 1105 100-101 1.5047617493880345 1106 1 0.5443351453552161 1106 2 0.55294490486774 1106 3 0.6016384052457084 1106 4 0.5000747891393758 1106 5 0.5696270099366458 1106 6 0.6260237304753531 1106 7 0.46799374355391166 1106 8 0.5331207530771209 1106 9 0.6872468742876734 1106 10-11 0.7818126988789373 1106 12-13 0.6535936622135736 1106 14-15 0.9373408207817349 1106 16-17 0.9704255123603289 1106 18-19 1.0179824168989953 1106 20-21 1.022420277259478 1106 22-23 1.1563885311823086 1106 24-25 1.0860677931780387 1106 26-27 1.0240861789352351 1106 28-29 0.915886812695156 1106 30-31 1.0169062985263153 1106 32-33 1.053037832222806 1106 34-35 1.0645943924092833 1106 36-37 0.9669197139429073 1106 38-39 1.057185595416989 1106 40-41 0.9976272686279657 1106 42-43 1.2086594914487208 1106 44-45 1.444958971420185 1106 46-47 1.1863460230683742 1106 48-49 1.279345920069268 1106 50-51 1.194358187415439 1106 52-53 1.2174690082285764 1106 54-55 1.228260894179094 1106 56-57 1.3295651395573742 1106 58-59 1.3571940555752136 1106 60-61 1.5389488355914125 1106 62-63 1.500279801053523 1106 64-65 1.437757012328376 1106 66-67 1.4721292403723183 1106 68-69 1.433702094028348 1106 70-71 1.4340016296051346 1106 72-73 1.342402933896544 1106 74-75 1.2878146085653661 1106 76-77 1.4008183407695327 1106 78-79 1.4594160990284735 1106 80-81 1.5569952530031372 1106 82-83 1.6742979867177787 1106 84-85 1.9003311722661032 1106 86-87 1.7678617390550144 1106 88-89 1.8708710654982994 1106 90-91 1.8094199870640786 1106 92-93 1.740124425767707 1106 94-95 1.8583399851525009 1106 96-97 1.8684150020688435 1106 98-99 2.091164108637024 1106 100-101 2.2564687486937807 1107 1 0.3064170911392985 1107 2 0.3370530363170552 1107 3 0.31153324485322287 1107 4 0.3172857975385952 1107 5 0.13912181293398618 1107 6 0.22506834561084332 1107 7 0.24522643644379372 1107 8 0.28347805434369633 1107 9 0.29687981030669164 1107 10-11 0.33919152740014624 1107 12-13 0.30429570933274874 1107 14-15 0.24712547783119732 1107 16-17 0.35965977291685647 1107 18-19 0.48123882192022194 1107 20-21 0.40440020737158733 1107 22-23 0.4849289819099383 1107 24-25 0.3605745531537181 1107 26-27 0.5176935957820135 1107 28-29 0.3691292776866675 1107 30-31 0.5456810778678474 1107 32-33 0.5181157109914807 1107 34-35 0.5286322839445887 1107 36-37 0.5712188284928672 1107 38-39 0.4857807267325356 1107 40-41 0.5415089097965833 1107 42-43 0.619836274024145 1107 44-45 0.6771526955906424 1107 46-47 0.6553858976550444 1107 48-49 0.5168325139380059 1107 50-51 0.6353238043556217 1107 52-53 0.6714095307684786 1107 54-55 0.8354719260463739 1107 56-57 0.8273732224278021 1107 58-59 0.8068662067283086 1107 60-61 0.7301762387797837 1107 62-63 0.7034702842844212 1107 64-65 0.7989694663603046 1107 66-67 0.7956646572003265 1107 68-69 0.8065395706482903 1107 70-71 0.7632408419230501 1107 72-73 0.6668667473160426 1107 74-75 0.9487394614792741 1107 76-77 0.8196289323759345 1107 78-79 0.8283354651091415 1107 80-81 0.8498055770736244 1107 82-83 0.8891118497722417 1107 84-85 0.9527404036635545 1107 86-87 0.863179508368642 1107 88-89 0.8383492578003064 1107 90-91 0.9303085583518431 1107 92-93 0.9790550095827193 1107 94-95 0.9527960071749284 1107 96-97 0.8715996182838666 1107 98-99 0.831168239016197 1107 100-101 1.0180309942119656 1108 1 -0.06551328849111471 1108 2 0.1542756530040208 1108 3 0.12078726502120674 1108 4 0.10873923864050994 1108 5 0.07508240406698974 1108 6 0.028744470847065884 1108 7 0.05204989977880814 1108 8 -0.09073756376211861 1108 9 -0.002024109119489026 1108 10-11 -0.006564686032668021 1108 12-13 -0.23252593905568375 1108 14-15 0.07812881218237067 1108 16-17 0.13523519069180878 1108 18-19 0.21843639724969677 1108 20-21 0.1526594528688605 1108 22-23 0.1174810911108537 1108 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-1.134516523518684 2316 70-71 -1.5458318042458643 2316 72-73 -1.3765856908542595 2316 74-75 -1.6336197366458087 2316 76-77 -1.8849117106620135 2316 78-79 -2.1250264886313097 2316 80-81 -2.0799399633611415 2316 82-83 -2.1046565457913076 2316 84-85 -1.6452824880847068 2316 86-87 -1.5564101361020484 2316 88-89 -1.9339680224714115 2316 90-91 -1.9614874780479141 2316 92-93 -2.369220785403158 2316 94-95 -2.366237421826778 2316 96-97 -2.3821315627399215 2316 98-99 -2.4652855655182115 2316 100-101 -2.3858320169157743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6046.0 3 1872.0 4 2091.0 5 2162.0 6 2234.0 7 2749.0 8 3423.0 9 4325.0 10 5229.0 11 6487.0 12 8195.0 13 9822.0 14 11079.0 15 13551.0 16 16878.0 17 22179.0 18 19559.0 19 13460.0 20 12564.0 21 13342.0 22 14167.0 23 15866.0 24 17945.0 25 20574.0 26 24235.0 27 28534.0 28 34051.0 29 41141.0 30 50396.0 31 62119.0 32 78675.0 33 102136.0 34 138236.0 35 198587.0 36 296268.0 37 434986.0 38 398729.0 39 72715.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07498509411676 18.824033497997057 14.60685874663626 22.494122661249918 2 15.196596036510693 20.306532699784643 40.91305759813927 23.583813665565394 3 19.080969726760443 25.297819189481974 29.960263567318634 25.66094751643895 4 12.552660820531017 16.88839603931645 37.9293118804626 32.62963125968993 5 13.575906143809943 37.323149823609114 34.13586611621158 14.965077916369355 6 30.926153405101164 36.296311752783836 18.146568226313622 14.630966615801382 7 27.883406249036007 33.016886442034284 21.18394717407044 17.91576013485927 8 26.03571337854657 34.39671532714282 20.13118552305476 19.436385771255846 9 26.440154937615745 16.882266103268154 20.778843702279516 35.89873525683659 10-11 24.417196783326357 26.871641076053276 27.712486317070173 20.9986758235502 12-13 25.779660574915837 24.423615749124338 28.095493495316376 21.70123018064345 14-15 22.72444301012548 25.413193854633686 26.93613216034668 24.92623097489415 16-17 21.637396127161495 28.818832341821594 27.283297147766856 22.26047438325006 18-19 21.500328364924094 27.104188739078694 30.12510068648225 21.270382209514963 20-21 22.473341089175022 26.16757651562577 30.086075331730015 21.273007063469194 22-23 22.063841573095107 26.244727919730828 30.081190206203217 21.610240300970847 24-25 21.95401308600215 26.425449236196446 30.15373558658027 21.466802091221133 26-27 21.738199005933794 26.536217674429146 30.057433676968152 21.668149642668904 28-29 21.433080092396516 26.59134131841881 30.32599518065296 21.649583408531715 30-31 21.6691674202175 26.278994327570075 30.32350509607405 21.728333156138376 32-33 21.525757575414108 26.50412810792541 30.174506947204062 21.79560736945642 34-35 22.009792587555253 26.462110393290267 29.90778646718803 21.62031055196645 36-37 21.987341178166766 26.234604633407166 29.971022219925096 21.807031968500972 38-39 21.724080085598736 26.50704557407374 30.05193503926298 21.716939301064546 40-41 21.92223517806321 26.452708623488874 29.952644801550043 21.672411396897868 42-43 21.784363406518967 26.527698302653164 29.691009783979812 21.99692850684806 44-45 21.91211840258966 26.600595277225548 29.500610918554017 21.98667540163078 46-47 22.06439612876143 26.83723580619146 29.0683568542492 22.030011210797905 48-49 22.10535358932052 26.953321481249098 28.84934516970143 22.09197975972895 50-51 21.98943256169835 27.067586865879477 28.767633078307515 22.175347494114657 52-53 22.188256244214124 27.232249313401656 28.48718969336323 22.09230474902099 54-55 22.045682309308898 27.04091405421371 28.54630897159607 22.36709466488132 56-57 22.08221100943636 27.410266605670884 28.421910966409854 22.0856114184829 58-59 22.24119478155876 27.101920654248 28.46096246040341 22.195922103789826 60-61 22.166061610125958 26.996267193331093 28.85382931194031 21.983841884602636 62-63 22.013053716845114 27.12279331131339 28.814416991188324 22.049735980653164 64-65 21.985320135161203 27.162351434994076 28.621565633131222 22.2307627967135 66-67 21.990731713952762 27.401343085287277 28.30935398595247 22.29857121480749 68-69 21.852870572867726 27.274007095245363 28.558419250608065 22.31470308127885 70-71 21.96898082475297 27.15742776504696 28.50382215364629 22.369769256553777 72-73 22.06779425172298 27.104964309809482 28.48672549725703 22.340515941210505 74-75 21.858549448038865 27.52906448333056 28.27995646503717 22.3324296035934 76-77 21.999342642125306 27.40518179345362 28.190815123764622 22.40466044065645 78-79 22.08704791866084 27.354727047410815 28.1270418788936 22.43118315503475 80-81 21.85114564377918 27.653463497253195 28.14874102040317 22.34664983856446 82-83 22.008629119048607 27.54090511211031 28.25853985112718 22.191925917713906 84-85 21.959853853243356 27.448065868066617 28.024037355165415 22.56804292352462 86-87 22.03584812532719 27.346115538638976 28.167874081515638 22.4501622545182 88-89 22.135744783913744 27.2710532498433 28.04331793974458 22.54988402649838 90-91 22.006201676225526 27.34074508535183 28.224519088960747 22.428534149461893 92-93 22.093976367481513 27.264851044571103 28.212954327832772 22.428218260114612 94-95 21.831211877341726 27.219603518684938 28.262428788762122 22.686755815211217 96-97 21.861361368058038 27.26672999848738 28.217659781126496 22.65424885232808 98-99 21.743408031160076 27.31882931941686 28.01509911630698 22.922663533116086 100-101 21.771434508171026 27.22674668393075 28.192089524002228 22.809729283895997 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 536.0 1 434.5 2 395.0 3 507.5 4 456.0 5 449.5 6 816.5 7 1716.5 8 3014.5 9 3614.0 10 3133.0 11 2692.5 12 2762.0 13 2973.5 14 3130.0 15 3307.5 16 3461.5 17 3587.0 18 3779.5 19 4024.0 20 4426.0 21 4987.5 22 5813.0 23 6985.0 24 8611.5 25 10644.0 26 13279.5 27 16360.5 28 18899.0 29 21582.5 30 25489.0 31 30133.5 32 34670.5 33 39504.0 34 45849.0 35 50620.5 36 55359.0 37 60667.0 38 63331.0 39 64778.5 40 66754.5 41 70810.0 42 73897.0 43 76781.5 44 80673.0 45 83544.5 46 83888.5 47 84789.5 48 90472.0 49 90086.0 50 83414.0 51 78867.0 52 74706.0 53 71198.5 54 67669.0 55 62536.5 56 56767.5 57 50606.5 58 45500.0 59 40771.0 60 34572.0 61 27833.5 62 21705.0 63 16643.5 64 12614.0 65 9603.5 66 6967.5 67 5176.5 68 3660.5 69 2316.5 70 1586.0 71 1103.0 72 760.5 73 546.0 74 408.0 75 320.0 76 227.0 77 123.0 78 58.0 79 33.5 80 30.5 81 22.0 82 15.5 83 13.0 84 10.0 85 8.0 86 7.0 87 8.0 88 6.0 89 6.0 90 6.0 91 2.5 92 1.5 93 1.5 94 3.5 95 4.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051933903458226974 2 0.08732690398255093 3 0.0620850329299921 4 0.12181355366118159 5 0.09842970184229405 6 0.09675295277776141 7 0.10327867886675329 8 0.09004595651963088 9 0.10916995936375982 10-11 0.10250828064791397 12-13 0.08580876631601463 14-15 0.08907162936051057 16-17 0.1030067736130453 18-19 0.08098244806269772 20-21 0.06487206178049904 22-23 0.0689053230438343 24-25 0.056239069975270214 26-27 0.04819520621974205 28-29 0.0622209855568461 30-31 0.04452448529468411 32-33 0.04438853266783012 34-35 0.04083110559848386 36-37 0.04511361334438477 38-39 0.04581603524979709 40-41 0.05875419357206917 42-43 0.05478890862216092 44-45 0.04332357042414047 46-47 0.033625616375222 48-49 0.03854256971310824 50-51 0.024222226351153852 52-53 0.03847459339968124 54-55 0.04157884504618084 56-57 0.0468583387223444 58-59 0.05105021138367598 60-61 0.048580405329161705 62-63 0.0555366480698579 64-65 0.03808939429026158 66-67 0.05805177166665684 68-69 0.04937346231914336 70-71 0.02263611237119055 72-73 0.016880784501038 74-75 0.02755306570907679 76-77 0.03856522848425057 78-79 0.05746264361695619 80-81 0.03702443204657193 82-83 0.05490220247787258 84-85 0.06043094263660181 86-87 0.05979649704461648 88-89 0.08324832517693101 90-91 0.05798379535322985 92-93 0.0684974651632723 94-95 0.07611081226709615 96-97 0.08435860496290533 98-99 0.1154917565124707 100-101 0.11148115402027779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2206651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.13406864913738 #Duplication Level Percentage of deduplicated Percentage of total 1 79.89631534064941 45.64801565485792 2 11.785229679388468 13.46676243096064 3 3.4531962160294074 5.918854489966967 4 1.480808906017439 3.384185507706175 5 0.7808587433207854 2.230681852308445 6 0.4823651296094655 1.6535689457434346 7 0.34294451585789854 1.371567085830925 8 0.24462641282169392 1.1181201810837504 9 0.19273138625664932 0.9910375427907734 >10 1.1802009324645872 12.876231963160652 >50 0.1082678501499765 4.274001863952353 >100 0.05006881060005722 4.886297344380621 >500 0.001749789678422318 0.6422785360922677 >1k 3.9767947236868997E-4 0.38003221999005454 >5k 1.59071788947476E-4 0.6343261698961105 >10k+ 7.9535894473738E-5 0.5240382112788843 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11532 0.5226018976267656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8553 0.38760093916074634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5406 0.2449866335909031 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2210 0.10015176844911135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.531754228466577E-5 2 0.0 0.0 0.0 0.0 4.531754228466577E-5 3 0.0 0.0 0.0 0.0 4.531754228466577E-5 4 0.0 0.0 0.0 0.0 4.531754228466577E-5 5 0.0 0.0 0.0 0.0 4.531754228466577E-5 6 0.0 0.0 0.0 0.0 9.063508456933153E-5 7 0.0 0.0 0.0 0.0 9.063508456933153E-5 8 0.0 0.0 0.0 0.0 9.063508456933153E-5 9 0.0 0.0 0.0 0.0 9.063508456933153E-5 10-11 0.0 0.0 0.0 0.0 9.063508456933153E-5 12-13 0.0 0.0 0.0 0.0 1.3595262685399731E-4 14-15 0.0 0.0 0.0 0.0 1.3595262685399731E-4 16-17 0.0 0.0 0.0 4.531754228466577E-5 1.3595262685399731E-4 18-19 2.2658771142332883E-5 0.0 0.0 4.531754228466577E-5 1.3595262685399731E-4 20-21 4.531754228466577E-5 0.0 0.0 6.797631342699866E-5 1.3595262685399731E-4 22-23 9.063508456933153E-5 0.0 0.0 2.2658771142332885E-4 2.0392894028099594E-4 24-25 1.3595262685399731E-4 0.0 0.0 4.5317542284665764E-4 2.2658771142332885E-4 26-27 1.3595262685399731E-4 0.0 0.0 9.74327159120314E-4 2.2658771142332885E-4 28-29 1.3595262685399731E-4 0.0 0.0 0.001971313089382961 2.2658771142332885E-4 30-31 1.3595262685399731E-4 4.531754228466577E-5 0.0 0.003330839357922934 2.2658771142332885E-4 32-33 1.3595262685399731E-4 9.063508456933153E-5 0.0 0.0050302471935979 2.2658771142332885E-4 34-35 1.3595262685399731E-4 9.063508456933153E-5 0.0 0.007001560282980861 2.2658771142332885E-4 36-37 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.00926743739721415 2.2658771142332885E-4 38-39 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.011012162775173782 2.2658771142332885E-4 40-41 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.013844509167965393 2.2658771142332885E-4 42-43 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.01796840551586998 2.2658771142332885E-4 44-45 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.024539449147146515 2.4924648256566175E-4 46-47 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.039924754752790545 2.7190525370799463E-4 48-49 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.06990230897409694 2.7190525370799463E-4 50-51 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.08841452499738292 2.945640248503275E-4 52-53 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.10724396381666154 4.078578805619919E-4 54-55 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.134366512874034 4.078578805619919E-4 56-57 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.15419293762357528 4.078578805619919E-4 58-59 1.8127016913866307E-4 9.063508456933153E-5 0.0 0.1799106428701231 4.078578805619919E-4 60-61 2.2658771142332885E-4 9.063508456933153E-5 0.0 0.22275837910027457 4.078578805619919E-4 62-63 2.4924648256566175E-4 9.063508456933153E-5 0.0 0.25028878603820903 4.078578805619919E-4 64-65 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.28309868665230703 4.078578805619919E-4 66-67 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.34835594754222576 4.078578805619919E-4 68-69 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.42331116248106293 4.078578805619919E-4 70-71 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.48757143744071896 4.078578805619919E-4 72-73 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.5639994725038078 4.078578805619919E-4 74-75 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.6292114158514419 4.078578805619919E-4 76-77 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.677678527324892 4.078578805619919E-4 78-79 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.7419388022845479 4.531754228466577E-4 80-81 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.8485030029669395 4.531754228466577E-4 82-83 2.7190525370799463E-4 9.063508456933153E-5 0.0 0.9549539097936194 4.984929651313234E-4 84-85 2.7190525370799463E-4 9.063508456933153E-5 0.0 1.0853324789466028 4.984929651313234E-4 86-87 2.7190525370799463E-4 9.063508456933153E-5 0.0 1.3302737949952212 4.984929651313234E-4 88-89 2.7190525370799463E-4 9.063508456933153E-5 0.0 1.6290976688203074 4.984929651313234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12245 0.0 35.045303 1 GGTATCA 7605 0.0 34.30631 1 ATCAACG 14710 0.0 29.10672 3 TCAACGC 14820 0.0 28.922743 4 CAACGCA 14905 0.0 28.820911 5 TATCAAC 14970 0.0 28.57269 2 AACGCAG 15170 0.0 28.348131 6 GTACATG 9990 0.0 26.686905 1 ACGCAGA 17000 0.0 25.04497 7 TACATGG 10325 0.0 25.040285 2 CGCAGAG 17105 0.0 24.864578 8 ACATGGG 10185 0.0 24.495111 3 GCAGAGT 17645 0.0 23.053307 9 GAGTACT 8640 0.0 22.574415 12-13 CATGGGG 4620 0.0 20.983086 4 AGAGTAC 14400 0.0 20.839016 10-11 GTACTTT 9360 0.0 20.71125 14-15 AGTACTT 8985 0.0 20.65 12-13 CATGGGA 6015 0.0 19.59283 4 GTGTAGC 1170 0.0 19.090303 1 >>END_MODULE