FastQCFastQC Report
Thu 26 May 2016
SRR937019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206651
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113800.5157136311994964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90960.41220836462131977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.26655778371840405No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29500.133686749739764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.11565036791046704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85400.034.3227921
GTATCAA129050.032.1742741
ATCAACG159250.025.7399643
TCAACGC160000.025.6489964
TATCAAC161050.025.6003252
CAACGCA162850.025.2584515
AACGCAG167150.024.608676
GTACATG104650.023.8782141
GTACCGT1401.780983E-523.7490716
TACATGG108650.022.6020222
ACATGGG107200.021.755213
ACGCAGA189200.021.6402747
GAGTACT96450.021.32320412-13
CGCAGAG190500.021.293138
AGTACTT102200.020.3094112-13
GCAGAGT200100.019.9629889
GTACTTT105300.019.689414-15
AGAGTAC163600.019.6261510-11
CATGGGG45650.019.3530224
CATGGGA63050.018.3061834
Produced by FastQC (version 0.11.5)