##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937019_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2206651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15486862217904 33.0 31.0 34.0 31.0 34.0 2 32.14592157980578 33.0 31.0 34.0 30.0 34.0 3 32.326869088043374 33.0 31.0 34.0 31.0 34.0 4 36.05734889658582 37.0 35.0 37.0 35.0 37.0 5 35.98688283738571 37.0 35.0 37.0 35.0 37.0 6 36.034675623829955 37.0 35.0 37.0 35.0 37.0 7 35.975753755351434 37.0 35.0 37.0 35.0 37.0 8 35.95930665973006 37.0 35.0 37.0 35.0 37.0 9 37.64324489917073 39.0 37.0 39.0 35.0 39.0 10-11 37.57230187283807 39.0 37.0 39.0 35.0 39.0 12-13 37.47718941509102 39.0 37.0 39.0 35.0 39.0 14-15 38.69036879869087 40.0 38.0 41.0 34.5 41.0 16-17 38.613176256689435 40.0 38.0 41.0 34.0 41.0 18-19 38.679648480888005 40.0 38.0 41.0 34.5 41.0 20-21 38.761623836302164 40.0 38.0 41.0 34.0 41.0 22-23 38.68980527505256 40.0 38.0 41.0 34.0 41.0 24-25 38.59428541260036 40.0 38.0 41.0 34.0 41.0 26-27 38.44244105660569 40.0 38.0 41.0 34.0 41.0 28-29 38.27261651253416 40.0 38.0 41.0 34.0 41.0 30-31 38.133832445638205 40.0 38.0 41.0 34.0 41.0 32-33 38.11096634673992 40.0 37.0 41.0 34.0 41.0 34-35 38.02422743786852 40.0 37.0 41.0 33.0 41.0 36-37 37.86497910181538 40.0 37.0 41.0 33.0 41.0 38-39 37.714595783383956 40.0 37.0 41.0 33.0 41.0 40-41 37.54016221867436 40.0 37.0 41.0 33.0 41.0 42-43 37.315444535633404 40.0 37.0 41.0 32.0 41.0 44-45 36.971202967755204 40.0 36.0 41.0 31.0 41.0 46-47 36.682298424173105 39.5 36.0 41.0 31.0 41.0 48-49 36.35183021692148 39.0 36.0 41.0 30.0 41.0 50-51 36.08916022515568 39.0 35.5 41.0 29.5 41.0 52-53 35.839863213530364 39.0 35.0 40.0 28.5 41.0 54-55 35.60196922848244 39.0 35.0 40.0 28.0 41.0 56-57 35.33804348761993 38.0 35.0 40.0 28.0 41.0 58-59 35.02185325182822 38.0 34.0 40.0 27.0 41.0 60-61 34.69400077311727 38.0 34.0 40.0 26.0 41.0 62-63 34.336732451121634 37.0 34.0 40.0 26.0 41.0 64-65 33.94032721984582 37.0 33.0 39.5 26.0 41.0 66-67 33.56072777253857 36.0 33.0 39.0 25.0 40.0 68-69 33.12544620785072 36.0 32.5 39.0 23.5 40.0 70-71 32.65062803315975 35.0 32.0 38.0 22.5 40.0 72-73 32.310638157098694 35.0 32.0 37.5 22.0 39.5 74-75 31.85893782025341 35.0 31.0 37.0 21.0 39.0 76-77 30.459922525129713 33.5 29.5 35.5 20.0 38.0 78-79 31.185348294768858 34.0 31.0 36.0 20.0 38.5 80-81 31.008048395509757 34.0 31.0 36.0 19.5 37.5 82-83 30.675291878960472 34.0 31.0 35.0 18.0 37.0 84-85 30.298488977187603 34.0 30.5 35.0 16.0 36.5 86-87 29.93839623936907 34.0 30.0 35.0 11.0 36.0 88-89 29.58902993722161 34.0 30.0 35.0 7.0 36.0 90-91 29.176737055383928 34.0 29.0 35.0 2.0 35.0 92-93 28.725235209373842 34.0 29.0 35.0 2.0 35.0 94-95 28.25952042257702 34.0 29.0 35.0 2.0 35.0 96-97 27.643235835662278 33.0 27.5 35.0 2.0 35.0 98-99 26.93034739068389 33.0 26.5 35.0 2.0 35.0 100-101 23.919934552405433 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21796926374086212 1101 2 0.12477969543178347 1101 3 0.17162378949453228 1101 4 0.23978996695060317 1101 5 0.1709768015119053 1101 6 0.1671767890473248 1101 7 0.191504421675738 1101 8 0.21568383427500493 1101 9 0.2520499110818335 1101 10-11 0.2516707939721812 1101 12-13 0.31374834883589386 1101 14-15 0.4067485001955049 1101 16-17 0.4351900453227273 1101 18-19 0.3326768714727848 1101 20-21 0.31331055796018603 1101 22-23 0.26400488905508723 1101 24-25 0.3071155743938405 1101 26-27 0.24817388158499654 1101 28-29 0.34032644353300867 1101 30-31 0.3820055195136334 1101 32-33 0.5049733199868243 1101 34-35 0.5464281030686422 1101 36-37 0.38241436138218177 1101 38-39 0.2505145502387265 1101 40-41 0.386385876446397 1101 42-43 0.46931785298918527 1101 44-45 0.4105274354069266 1101 46-47 0.3594408749454914 1101 48-49 0.29733173650547684 1101 50-51 0.29237619733084586 1101 52-53 0.1608623224677288 1101 54-55 0.19061587841179062 1101 56-57 0.12177298909139012 1101 58-59 0.1751132777772213 1101 60-61 0.2364877490495232 1101 62-63 0.16976985998844896 1101 64-65 0.2097859410501357 1101 66-67 0.34982776383442626 1101 68-69 0.23437666678980662 1101 70-71 0.2523502149506953 1101 72-73 0.3806101887012616 1101 74-75 0.22254058649944497 1101 76-77 0.037412478549054384 1101 78-79 0.1385521853988223 1101 80-81 0.3616549923601404 1101 82-83 0.4104077712173506 1101 84-85 0.44600513314820844 1101 86-87 0.29165628923643894 1101 88-89 0.362710807939127 1101 90-91 0.4762798499837366 1101 92-93 0.5360537950249942 1101 94-95 0.6491940517176573 1101 96-97 0.3723470105903175 1101 98-99 0.16129171672804077 1101 100-101 0.4195085303841388 1102 1 0.26236606170850507 1102 2 0.114032446858225 1102 3 0.1977862755374531 1102 4 0.1984377630909222 1102 5 0.2659936997262662 1102 6 0.1958513528417285 1102 7 0.23457000755011137 1102 8 0.21589028483049333 1102 9 0.3106454641999079 1102 10-11 0.3366444242476021 1102 12-13 0.27816470444776087 1102 14-15 0.4308159858877687 1102 16-17 0.41222951283789655 1102 18-19 0.34688697352397924 1102 20-21 0.3291025282943494 1102 22-23 0.32497627831760667 1102 24-25 0.3040422190443621 1102 26-27 0.3499851306187338 1102 28-29 0.31693007974191545 1102 30-31 0.3924092879454548 1102 32-33 0.36348944479958334 1102 34-35 0.3567145413946946 1102 36-37 0.36001439731403195 1102 38-39 0.34395099853271205 1102 40-41 0.37748596635482556 1102 42-43 0.5089859088632167 1102 44-45 0.5917767606997231 1102 46-47 0.6446534205202354 1102 48-49 0.6144613015645817 1102 50-51 0.5986586768492899 1102 52-53 0.6245789525327652 1102 54-55 0.5160775357369687 1102 56-57 0.5083538605806552 1102 58-59 0.44832978531148626 1102 60-61 0.4078807938721454 1102 62-63 0.5325792107802556 1102 64-65 0.446077033181453 1102 66-67 0.6138040330522543 1102 68-69 0.5225649370887453 1102 70-71 0.33965114185065204 1102 72-73 0.47513491509517536 1102 74-75 0.18024091642634588 1102 76-77 0.2092424578488412 1102 78-79 0.45063576793058147 1102 80-81 0.4006947136046115 1102 82-83 0.5307853866188772 1102 84-85 0.46541353410985664 1102 86-87 0.4087784472908851 1102 88-89 0.5789929013477568 1102 90-91 0.8256939480351413 1102 92-93 0.640559696595357 1102 94-95 0.7074911971053375 1102 96-97 0.8868399971812728 1102 98-99 0.8016202567837816 1102 100-101 1.0580144745053808 1103 1 -0.03498400958245185 1103 2 -0.2295478187637272 1103 3 -0.09165113484527865 1103 4 0.09298292802699137 1103 5 0.1116183315342738 1103 6 0.09411554742745665 1103 7 0.1808585238026197 1103 8 0.21140302965783064 1103 9 0.12831911369259785 1103 10-11 0.17386118563316444 1103 12-13 0.2204169377713967 1103 14-15 0.19674837584388172 1103 16-17 0.24507739829636677 1103 18-19 0.2917093070392127 1103 20-21 0.24019460162626416 1103 22-23 0.27564606622972576 1103 24-25 0.18612626397021614 1103 26-27 0.233215935749449 1103 28-29 0.23652229423023385 1103 30-31 0.2356604468689696 1103 32-33 0.2967323047801713 1103 34-35 0.2794307128983249 1103 36-37 0.3658658028506494 1103 38-39 0.3364374802116643 1103 40-41 0.3689629705665851 1103 42-43 0.2603678780926657 1103 44-45 0.3281416873464238 1103 46-47 0.38946549656682095 1103 48-49 0.41483414970415566 1103 50-51 0.21498565396088054 1103 52-53 0.26730062774164054 1103 54-55 0.27302937242814806 1103 56-57 0.20752221539114402 1103 58-59 0.31272008416330266 1103 60-61 0.2889828643623318 1103 62-63 0.30592719277049696 1103 64-65 0.09986042767223324 1103 66-67 0.19980926653052933 1103 68-69 0.30040761169115626 1103 70-71 0.1710704191360648 1103 72-73 0.2218383475045016 1103 74-75 0.18857611686689424 1103 76-77 0.13129259114944958 1103 78-79 0.14654846106771657 1103 80-81 0.296119433236381 1103 82-83 0.29577461627678403 1103 84-85 0.3479108217686324 1103 86-87 0.2153254548525929 1103 88-89 0.39103997290242987 1103 90-91 0.6155774498419753 1103 92-93 0.5452279914414895 1103 94-95 0.4942438467706367 1103 96-97 0.7195581922882468 1103 98-99 0.8193792354002092 1103 100-101 0.9002733550405786 1104 1 0.14353875897502633 1104 2 0.08049465620249663 1104 3 0.05934159401643768 1104 4 0.007867582626992942 1104 5 -0.02147363505498845 1104 6 0.08804047204569088 1104 7 0.12609729271503056 1104 8 0.1151614865625703 1104 9 0.1669008482851666 1104 10-11 0.12394464550963136 1104 12-13 0.19286806309849425 1104 14-15 0.1866195948268654 1104 16-17 0.17637292662166004 1104 18-19 0.2058366281362325 1104 20-21 0.19865502222386056 1104 22-23 0.10471437943613893 1104 24-25 0.1613839683129754 1104 26-27 0.1559597147657783 1104 28-29 0.19435345916938473 1104 30-31 0.2713648666428554 1104 32-33 0.22346834555078487 1104 34-35 0.0404600569556024 1104 36-37 0.07313811309034435 1104 38-39 0.012440212539019058 1104 40-41 0.2136773737154627 1104 42-43 0.1457176980302819 1104 44-45 0.11274539105632897 1104 46-47 0.2079191700570462 1104 48-49 0.30700943051273555 1104 50-51 0.24766927824577323 1104 52-53 0.15570866198948607 1104 54-55 0.10864815583930465 1104 56-57 -0.050702223870665364 1104 58-59 0.015662275014356908 1104 60-61 0.17136797518733715 1104 62-63 0.21279777387557886 1104 64-65 0.04044779434152446 1104 66-67 0.07016058489521271 1104 68-69 0.14284448940443895 1104 70-71 0.07350423302639086 1104 72-73 0.05794106426160539 1104 74-75 0.06911297231304658 1104 76-77 0.04186286403059469 1104 78-79 0.04467433898692619 1104 80-81 0.18245269011366005 1104 82-83 0.1841609693731705 1104 84-85 0.2456769097689957 1104 86-87 0.11885881285276767 1104 88-89 0.29502612375302206 1104 90-91 0.34250477026930426 1104 92-93 0.36901363768211226 1104 94-95 0.2645545652957182 1104 96-97 0.5722482455786739 1104 98-99 0.44133437299599976 1104 100-101 0.5303709788198638 1105 1 0.12658100360363989 1105 2 -0.023736842993081098 1105 3 0.08380787856579275 1105 4 0.008729438469622153 1105 5 0.12249945952475372 1105 6 0.09896086258125081 1105 7 0.13876649753320436 1105 8 0.0494432670213385 1105 9 0.12482851477053458 1105 10-11 0.19498965522738132 1105 12-13 0.22066395996485966 1105 14-15 0.36148561922202305 1105 16-17 0.36885669551041644 1105 18-19 0.31707412913463173 1105 20-21 0.27161172785026366 1105 22-23 0.32489496600016565 1105 24-25 0.31798490547661373 1105 26-27 0.333916971888641 1105 28-29 0.2134744118509886 1105 30-31 0.3958870615226786 1105 32-33 0.36762229293421456 1105 34-35 0.3547231816204217 1105 36-37 0.3501082866405767 1105 38-39 0.31450423557254936 1105 40-41 0.18437246843973298 1105 42-43 0.28169580552265217 1105 44-45 0.4042936127655068 1105 46-47 0.5017310945514808 1105 48-49 0.48491467581455794 1105 50-51 0.5682735662258196 1105 52-53 0.5657964830460074 1105 54-55 0.40535515147677614 1105 56-57 0.5124062764012862 1105 58-59 0.5123945634203793 1105 60-61 0.5801998106055848 1105 62-63 0.49629728513561133 1105 64-65 0.5954310402594913 1105 66-67 0.483781501444291 1105 68-69 0.40949663967010963 1105 70-71 0.3262876736796727 1105 72-73 0.3645251712747424 1105 74-75 0.42224661321658274 1105 76-77 0.32076555723502054 1105 78-79 0.40405234606890517 1105 80-81 0.6530477547908831 1105 82-83 0.7338454753654631 1105 84-85 0.6646205278139625 1105 86-87 0.790446761323981 1105 88-89 0.8540925353367292 1105 90-91 0.9327421419512909 1105 92-93 0.9410945098832677 1105 94-95 0.8322806122832134 1105 96-97 0.9865895306034567 1105 98-99 1.0572807028821636 1105 100-101 1.0903854888818216 1106 1 0.442578965933798 1106 2 0.277117445580636 1106 3 0.29698330751332236 1106 4 0.2635671011000227 1106 5 0.20685493373380126 1106 6 0.274122929463708 1106 7 0.27356737391156116 1106 8 0.26886424420123944 1106 9 0.4398712066586299 1106 10-11 0.44934111721041603 1106 12-13 0.4394625912998933 1106 14-15 0.6123236785669022 1106 16-17 0.6345577981963544 1106 18-19 0.6382994809933251 1106 20-21 0.5220661462578136 1106 22-23 0.49723495031190623 1106 24-25 0.5204068924752008 1106 26-27 0.5596249719968327 1106 28-29 0.6373327306832053 1106 30-31 0.547802110525943 1106 32-33 0.446171309713435 1106 34-35 0.46043744801419706 1106 36-37 0.4731652872987624 1106 38-39 0.5417019436447887 1106 40-41 0.5099066805557584 1106 42-43 0.5843898575102813 1106 44-45 0.5288227397231609 1106 46-47 0.36215454218065446 1106 48-49 0.2174617180376046 1106 50-51 0.29712688104581275 1106 52-53 0.3679310774505282 1106 54-55 0.5539616918560029 1106 56-57 0.3081163502442763 1106 58-59 0.32160690484728605 1106 60-61 0.5190108843493917 1106 62-63 0.4999693735668842 1106 64-65 0.5359901371930746 1106 66-67 0.6437929069856736 1106 68-69 0.6514478437887234 1106 70-71 0.6420500867856802 1106 72-73 0.6228178564006512 1106 74-75 0.5769521879566888 1106 76-77 0.6963759565642142 1106 78-79 0.6154653258454807 1106 80-81 0.8088959123226012 1106 82-83 0.8609830000474155 1106 84-85 0.7675641041950207 1106 86-87 0.5522167841350658 1106 88-89 0.9813689100876566 1106 90-91 1.1160251346210899 1106 92-93 0.9123690002403997 1106 94-95 1.1419532326725346 1106 96-97 1.3627427074750003 1106 98-99 1.473877118025836 1106 100-101 1.3650338555902621 1107 1 0.22694876629098104 1107 2 0.14007029120067926 1107 3 0.1293908257421421 1107 4 0.1526844907003806 1107 5 0.21766385441409142 1107 6 0.1328630230074097 1107 7 0.14536627138194547 1107 8 0.1311168028068579 1107 9 0.21099140773299752 1107 10-11 0.2065516797164335 1107 12-13 0.1966478082973353 1107 14-15 0.25359097746319037 1107 16-17 0.26593190024956925 1107 18-19 0.28251882759550284 1107 20-21 0.21872053912728262 1107 22-23 0.1893142442048159 1107 24-25 0.3062738379424559 1107 26-27 0.27417000838406835 1107 28-29 0.20268295632863698 1107 30-31 0.2313152092255777 1107 32-33 0.26359928035958546 1107 34-35 0.2563134052178455 1107 36-37 0.25558348349646565 1107 38-39 0.336706414310882 1107 40-41 0.24514538596349666 1107 42-43 0.27230896778905134 1107 44-45 0.310041952557377 1107 46-47 0.3162068900430697 1107 48-49 0.14560391572787523 1107 50-51 0.23549657814351832 1107 52-53 0.3258597626992028 1107 54-55 0.20903959409274364 1107 56-57 0.18875601925071095 1107 58-59 0.26396629007790295 1107 60-61 0.21421096132464612 1107 62-63 0.2891911960497566 1107 64-65 0.22381889542742783 1107 66-67 0.3137512042368584 1107 68-69 0.2587149643277442 1107 70-71 0.3390582607121857 1107 72-73 0.28542806829379685 1107 74-75 0.40112171186008894 1107 76-77 0.37204508972435235 1107 78-79 0.4852823453544808 1107 80-81 0.4708193313378324 1107 82-83 0.3640093241742264 1107 84-85 0.42794438069574525 1107 86-87 0.431293362773026 1107 88-89 0.4022818881614434 1107 90-91 0.5816160252575706 1107 92-93 0.6225497982014971 1107 94-95 0.5064836015200882 1107 96-97 0.7746144967505622 1107 98-99 0.8202598680604503 1107 100-101 0.7832062332101799 1108 1 -0.02585918906132889 1108 2 -0.1710219755326996 1108 3 -0.008637700803291182 1108 4 0.015977931998513384 1108 5 0.04818218108240302 1108 6 0.009488963744495038 1108 7 0.03480781967852664 1108 8 0.048476644170719396 1108 9 0.03478339523439189 1108 10-11 0.018067638369984707 1108 12-13 0.02419354679052077 1108 14-15 0.03853268456761327 1108 16-17 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-1.343872277037029 2316 50-51 -1.382557451600654 2316 52-53 -1.1619559658223295 2316 54-55 -1.1632010713746368 2316 56-57 -1.0236097474924009 2316 58-59 -0.7757735628949121 2316 60-61 -0.6103894858906713 2316 62-63 -0.6270529162255514 2316 64-65 -0.7131864555931102 2316 66-67 -0.8520391698768393 2316 68-69 -0.9855083137559646 2316 70-71 -0.8576529672760742 2316 72-73 -0.7273835977549155 2316 74-75 -0.7664389849169382 2316 76-77 -1.0901896479657829 2316 78-79 -0.7955549703465756 2316 80-81 -0.7220262534980435 2316 82-83 -0.5605265240014354 2316 84-85 -0.6300588624163588 2316 86-87 -0.5954068107117259 2316 88-89 -0.7204345443694642 2316 90-91 -0.6960608582421557 2316 92-93 -0.98518297576938 2316 94-95 -0.8574641836883892 2316 96-97 -0.6736765182039122 2316 98-99 -0.7498804417402916 2316 100-101 -0.5303674611911617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 12.0 5 67.0 6 85.0 7 228.0 8 589.0 9 1121.0 10 1868.0 11 3056.0 12 4814.0 13 6767.0 14 8344.0 15 11696.0 16 15926.0 17 22526.0 18 20365.0 19 12340.0 20 10581.0 21 10716.0 22 11095.0 23 12151.0 24 14038.0 25 16634.0 26 20574.0 27 25760.0 28 32585.0 29 40633.0 30 50937.0 31 64161.0 32 81857.0 33 107509.0 34 146516.0 35 210163.0 36 323762.0 37 498133.0 38 386792.0 39 32240.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94976070808108 19.76158284839648 15.039924289568829 22.248732153953608 2 15.509587191095765 21.250617468582746 39.76533927915924 23.474456061162247 3 19.345773554499534 25.475141461593054 30.065675521592887 25.11340946231452 4 13.098367705965016 17.583041072685326 37.21153658783069 32.10705463351896 5 13.581186843624609 37.81787217226662 33.859022779573884 14.741918204534887 6 31.132023741061644 36.83875174983153 17.511593412793474 14.517631096313355 7 27.91655771574209 33.42390799451295 20.86469496082525 17.794839328919707 8 26.34545578138808 34.83977975664469 19.693426687512748 19.12133777445449 9 26.52675932895596 16.97250720662216 20.464858285247644 36.03587517917423 10-11 24.57928845486719 27.255722875664155 27.26186340960058 20.903125259868077 12-13 25.95091449031763 24.641275713879796 27.917411955467113 21.490397840335458 14-15 22.775735718969607 25.787380967810495 26.62795340087762 24.80892991234228 16-17 21.642887796937533 29.215607724103176 27.054164886064903 22.087339592894388 18-19 21.494404869641823 27.389922556851992 29.977350292366122 21.138322281140063 20-21 22.440290438040822 26.330668295720383 30.155447435373368 21.07359383086543 22-23 22.106531572051946 26.512914819787998 30.04016040597267 21.34039320218739 24-25 22.050261909655276 26.664050251486294 30.14321021000131 21.142477628857126 26-27 21.858817110204644 26.735793725496947 30.069590816332543 21.335798347965866 28-29 21.496169527388858 26.839276047587635 30.267021635049513 21.397532789973994 30-31 21.797930285075868 26.51986262332344 30.229644762769155 21.45256232883154 32-33 21.648825792370925 26.819567513823074 30.12883405834308 21.402772635462924 34-35 22.053452932356656 26.66680413333572 29.949213618898433 21.33052931540919 36-37 22.178091646677238 26.437278815254174 29.862869422994738 21.52176011507385 38-39 21.77795844711083 26.75550431555742 30.023368430164016 21.44316880716774 40-41 22.015244045443453 26.75348782826169 29.86764731541062 21.36362081088424 42-43 21.95196505758628 26.768171151843422 29.560042998238924 21.719820792331376 44-45 22.015623739600716 26.914062181359732 29.322540765561428 21.747773313478127 46-47 22.22520311350066 27.187299469602767 28.852494824729636 21.735002592166943 48-49 22.328781556637388 27.296453103633457 28.602302814563725 21.772462525165437 50-51 22.221439235619776 27.45119809412501 28.423803407018916 21.903559263236293 52-53 22.423354052016574 27.680277129288257 28.145784724942878 21.750584093752288 54-55 22.221451800611263 27.366395296983935 28.302570851596858 22.109582050807948 56-57 22.25972767671421 27.782870986775382 28.145072920754703 21.812328415755704 58-59 22.436586502119976 27.451591296765926 28.18415700335535 21.927665197758753 60-61 22.323976294141172 27.471220221809073 28.5235727371294 21.681230746920356 62-63 22.214545934087447 27.523586647820615 28.50067817702029 21.76118924107165 64-65 22.15529311106242 27.56175490727672 28.298846235679342 21.984105745981516 66-67 22.17168056291651 27.836283996944687 27.992131060174685 21.999904379964114 68-69 22.067586144702116 27.605413725463094 28.257737235688207 22.06926289414658 70-71 22.151461333451763 27.57989603240026 28.21692530166782 22.051717332480152 72-73 22.292411574105543 27.464912876985476 28.129812158702105 22.112863390206876 74-75 22.16328150951468 27.91688551342469 27.880314124214927 22.039518852845703 76-77 22.184001676759323 27.865564688355022 27.838509950093755 22.111923684791908 78-79 22.31523683605296 27.818439099219745 27.65688143865624 22.209442626071056 80-81 22.187106778217306 28.05788179321256 27.652131863968403 22.10287956460173 82-83 22.301363245286492 28.04374765902168 27.70694135051988 21.94794774517195 84-85 22.191458125290467 27.942788296712123 27.592018676896085 22.27373490110132 86-87 22.210046680212333 27.774443099243435 27.7233919277516 22.292118292792633 88-89 22.25461208040248 27.832704582363053 27.520009643693182 22.392673693541287 90-91 22.151297558057596 27.9051229354971 27.61284665181758 22.330732854627726 92-93 22.25033421702581 27.791335281988534 27.632542541862097 22.32578795912356 94-95 22.0391540009485 27.7001901755402 27.65185889622959 22.608796927281706 96-97 22.045264363122786 27.7443341372043 27.679778230406804 22.53062326926611 98-99 21.980754049000407 27.787576690591926 27.37082580665043 22.860843453757237 100-101 21.7202722253756 28.087024203224153 27.41255603857017 22.780147532830078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 751.0 1 537.5 2 393.0 3 509.0 4 476.5 5 448.0 6 761.5 7 1587.0 8 2729.5 9 3361.5 10 3161.0 11 2829.5 12 2864.0 13 3062.5 14 3277.0 15 3497.5 16 3742.5 17 3934.0 18 4076.5 19 4354.0 20 4727.0 21 5291.5 22 6134.5 23 7339.5 24 8842.0 25 10718.0 26 13398.0 27 16536.5 28 19017.0 29 21800.0 30 25771.0 31 30150.5 32 34757.5 33 39509.0 34 45744.5 35 50531.5 36 55169.5 37 60720.5 38 63301.5 39 64780.0 40 66714.0 41 70466.0 42 73589.0 43 76841.0 44 81150.0 45 83876.0 46 83749.0 47 84760.5 48 90792.5 49 89842.5 50 83371.0 51 78949.0 52 74340.0 53 70489.5 54 66863.5 55 62325.0 56 56378.5 57 50073.0 58 45244.5 59 40580.5 60 34649.5 61 27982.0 62 21761.0 63 16670.0 64 12266.0 65 9218.0 66 6935.5 67 5399.5 68 3788.0 69 2253.5 70 1534.5 71 1039.5 72 754.0 73 589.0 74 443.5 75 315.0 76 190.0 77 99.5 78 53.5 79 34.5 80 20.0 81 11.5 82 11.0 83 8.0 84 4.5 85 2.5 86 1.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01586113979963302 2 0.002764370079364612 3 0.0020392894028099596 4 9.063508456933153E-5 5 0.0011329385571166443 6 4.531754228466577E-4 7 0.0 8 4.531754228466577E-5 9 0.0 10-11 1.1329385571166442E-4 12-13 5.438105074159891E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0024018297410872856 22-23 0.0 24-25 0.003149569188784271 26-27 0.0017220666068172993 28-29 0.003149569188784271 30-31 0.013980461794819389 32-33 6.344455919853208E-4 34-35 0.00251512359679895 36-37 0.003081592875357272 38-39 0.0018806780048136293 40-41 0.0038293323230542574 42-43 0.0022205595719486226 44-45 4.078578805619919E-4 46-47 1.359526268539973E-4 48-49 1.586113979963302E-4 50-51 2.2658771142332883E-5 52-53 0.0012462324128283085 54-55 0.0030362753330726066 56-57 4.078578805619919E-4 58-59 0.0010423034725473125 60-61 0.0010649622436896455 62-63 0.0 64-65 2.0392894028099594E-4 66-67 2.2658771142332883E-5 68-69 2.2658771142332883E-5 70-71 9.063508456933153E-5 72-73 4.531754228466577E-5 74-75 3.6254033827732614E-4 76-77 6.117868208429879E-4 78-79 3.85199109419659E-4 80-81 0.005415446303017559 82-83 0.0018806780048136293 84-85 0.0033761569002076 86-87 0.0018806780048136293 88-89 0.0012462324128283085 90-91 2.719052537079946E-4 92-93 4.531754228466577E-5 94-95 2.492464825656617E-4 96-97 0.0016540902933903005 98-99 0.0025830999102259486 100-101 0.0010649622436896455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2206651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.04030098433584 #Duplication Level Percentage of deduplicated Percentage of total 1 78.46471265024738 43.187214009190406 2 12.527500859422469 13.790348357682774 3 3.7265517798943075 6.153315947972854 4 1.6362221382365871 3.602326358631013 5 0.8558693929042281 2.3553654494364755 6 0.5230046599434197 1.727180033969762 7 0.3568516508572067 1.374885558895645 8 0.27993417073057575 1.2326128810249077 9 0.20148337841445815 0.9980735212145351 >10 1.2490676709733028 13.16160456761892 >50 0.11959062340452999 4.552752298463428 >100 0.05607942672842685 5.363726012673166 >500 0.00255472488243849 0.9337145266812074 >1k 3.2964192031463584E-4 0.3713085798111352 >5k 1.6482096015731792E-4 0.679386746690836 >10k+ 8.241048007865896E-5 0.5161851500428437 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11380 0.5157136311994964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9096 0.41220836462131977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5882 0.26655778371840405 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2950 0.133686749739764 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2552 0.11565036791046704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.2658771142332883E-5 16-17 0.0 0.0 0.0 4.531754228466577E-5 4.531754228466577E-5 18-19 0.0 0.0 0.0 4.531754228466577E-5 4.531754228466577E-5 20-21 0.0 0.0 0.0 1.1329385571166442E-4 9.063508456933153E-5 22-23 0.0 0.0 0.0 2.7190525370799463E-4 9.063508456933153E-5 24-25 0.0 0.0 0.0 5.438105074159893E-4 9.063508456933153E-5 26-27 0.0 0.0 0.0 0.0011329385571166443 9.063508456933153E-5 28-29 0.0 4.531754228466577E-5 0.0 0.002175242029663957 9.063508456933153E-5 30-31 0.0 4.531754228466577E-5 0.0 0.003512109527061597 9.063508456933153E-5 32-33 0.0 4.531754228466577E-5 0.0 0.005256834905021228 9.063508456933153E-5 34-35 2.2658771142332883E-5 4.531754228466577E-5 0.0 0.007318783078973522 9.063508456933153E-5 36-37 4.531754228466577E-5 4.531754228466577E-5 0.0 0.009607318964349142 9.063508456933153E-5 38-39 9.063508456933153E-5 4.531754228466577E-5 0.0 0.011284068028881777 9.063508456933153E-5 40-41 9.063508456933153E-5 4.531754228466577E-5 0.0 0.014184390735100385 9.063508456933153E-5 42-43 9.063508456933153E-5 4.531754228466577E-5 0.0 0.018602851107855298 1.1329385571166442E-4 44-45 9.063508456933153E-5 4.531754228466577E-5 0.0 0.0252192122814165 1.3595262685399731E-4 46-47 9.063508456933153E-5 4.531754228466577E-5 0.0 0.040672494200487526 1.3595262685399731E-4 48-49 9.063508456933153E-5 4.531754228466577E-5 0.0 0.07098992998892892 1.3595262685399731E-4 50-51 9.063508456933153E-5 4.531754228466577E-5 0.0 0.08970607495249588 1.3595262685399731E-4 52-53 9.063508456933153E-5 4.531754228466577E-5 0.0 0.10846753745834752 2.2658771142332885E-4 54-55 9.063508456933153E-5 4.531754228466577E-5 0.0 0.135522110202293 3.172227959926604E-4 56-57 9.063508456933153E-5 4.531754228466577E-5 0.0 0.15546182880754592 3.172227959926604E-4 58-59 9.063508456933153E-5 4.531754228466577E-5 0.0 0.18124751036752074 3.172227959926604E-4 60-61 9.063508456933153E-5 4.531754228466577E-5 0.0 0.22427651676681087 3.3988156713499326E-4 62-63 9.063508456933153E-5 4.531754228466577E-5 0.0 0.25171628862017603 3.6254033827732614E-4 64-65 9.063508456933153E-5 4.531754228466577E-5 0.0 0.2845035304631317 3.6254033827732614E-4 66-67 9.063508456933153E-5 4.531754228466577E-5 0.0 0.35010067292018543 3.6254033827732614E-4 68-69 9.063508456933153E-5 4.531754228466577E-5 0.0 0.42498791154559556 3.6254033827732614E-4 70-71 9.063508456933153E-5 4.531754228466577E-5 0.0 0.4895200917589596 4.078578805619919E-4 72-73 9.063508456933153E-5 4.531754228466577E-5 0.0 0.5656309040260558 4.3051665170432477E-4 74-75 9.063508456933153E-5 4.531754228466577E-5 0.0 0.6306615772045512 4.531754228466577E-4 76-77 9.063508456933153E-5 4.531754228466577E-5 0.0 0.6790607123645742 4.531754228466577E-4 78-79 9.063508456933153E-5 4.531754228466577E-5 0.0 0.7433663048665149 4.531754228466577E-4 80-81 1.3595262685399731E-4 9.063508456933153E-5 0.0 0.8505196335986072 4.531754228466577E-4 82-83 1.3595262685399731E-4 9.063508456933153E-5 0.0 0.9567439527138637 4.531754228466577E-4 84-85 1.3595262685399731E-4 9.063508456933153E-5 0.0 1.087258474493701 4.531754228466577E-4 86-87 1.3595262685399731E-4 9.063508456933153E-5 0.0 1.3334913404974325 4.531754228466577E-4 88-89 1.3595262685399731E-4 9.063508456933153E-5 0.0 1.6318620388996719 4.984929651313234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8540 0.0 34.322792 1 GTATCAA 12905 0.0 32.174274 1 ATCAACG 15925 0.0 25.739964 3 TCAACGC 16000 0.0 25.648996 4 TATCAAC 16105 0.0 25.600325 2 CAACGCA 16285 0.0 25.258451 5 AACGCAG 16715 0.0 24.60867 6 GTACATG 10465 0.0 23.878214 1 GTACCGT 140 1.780983E-5 23.749071 6 TACATGG 10865 0.0 22.602022 2 ACATGGG 10720 0.0 21.75521 3 ACGCAGA 18920 0.0 21.640274 7 GAGTACT 9645 0.0 21.323204 12-13 CGCAGAG 19050 0.0 21.29313 8 AGTACTT 10220 0.0 20.30941 12-13 GCAGAGT 20010 0.0 19.962988 9 GTACTTT 10530 0.0 19.6894 14-15 AGAGTAC 16360 0.0 19.62615 10-11 CATGGGG 4565 0.0 19.353022 4 CATGGGA 6305 0.0 18.306183 4 >>END_MODULE