FastQCFastQC Report
Thu 26 May 2016
SRR937018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943608
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106100.5458919699857173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78610.4044539845483246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.2549896892789081No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.10393042218389717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68750.037.8774381
GTATCAA111600.036.235821
ATCAACG136450.029.4988443
TCAACGC137050.029.335784
TATCAAC138700.029.0873372
CAACGCA138550.028.9518135
AACGCAG142350.028.3778136
ACGCAGA157050.025.5999267
CGCAGAG157800.025.4193338
GTACATG88700.024.804481
TACATGG89300.024.2121142
GCAGAGT165300.023.6047279
GAGTACT81450.023.6002712-13
ACATGGG91300.023.1624433
GTACTTT86950.021.94319714-15
AGAGTAC135050.021.51914210-11
AGTACTT86750.021.06281312-13
CATGGGA55250.020.2135164
CATGGGG37450.019.7960744
CAGAGTA159750.019.66453410-11
Produced by FastQC (version 0.11.5)