FastQCFastQC Report
Thu 26 May 2016
SRR937018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943608
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102460.5271639137109952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83260.42837856193224144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54520.28050923848841947No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27860.1433416614872958No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22270.11458071792254404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81300.034.6541981
GTATCAA123650.031.8147091
ATCAACG151050.025.8148063
TATCAAC151700.025.7681372
TCAACGC152950.025.4604534
CAACGCA153850.025.3115145
AACGCAG158150.024.6833656
GTACATG93000.022.8358041
GAGTACT90250.022.4429112-13
ACGCAGA176850.022.0196577
CGCAGAG177450.021.8649148
TACATGG96500.021.3122522
GTACTTT95750.020.90630514-15
ACATGGG94900.020.8196563
GCAGAGT185450.020.5887999
AGTACTT93750.020.49059712-13
AGAGTAC147600.020.33445210-11
TGCACCG4250.018.9959835
CGTCGAT901.2349422E-418.4709344-45
ACTTTTT113600.017.72603616-17
Produced by FastQC (version 0.11.5)