FastQCFastQC Report
Thu 26 May 2016
SRR937017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021629
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106470.5266544949642096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78140.3865199796797533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.24391221138992367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.037.0549771
GTATCAA117300.035.6063421
ATCAACG141850.029.04273
TATCAAC142850.028.939182
TCAACGC143250.028.7588624
CAACGCA145350.028.376055
AACGCAG149700.027.6446656
ACGCAGA166850.024.4038777
CGCAGAG167400.024.32378
GAGTACT86100.024.22240312-13
GTACATG93850.023.6438981
GCAGAGT175600.022.6985829
TACATGG96300.022.5988542
GTACTTT91550.022.59657914-15
ACATGGG96850.021.5873973
AGTACTT90100.020.80070712-13
AGAGTAC141450.020.47041510-11
GTGTAGG8800.019.9781631
CAGAGTA170550.018.99715210-11
CGATCGT2250.018.99567424-25
Produced by FastQC (version 0.11.5)