FastQCFastQC Report
Thu 26 May 2016
SRR937017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021629
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105170.5202240371502387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83720.4141214832197203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54320.2686942065037651No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27590.13647410083650363No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.1156493105312597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88350.032.6905561
GTATCAA129900.029.511391
ATCAACG156300.024.3091833
TCAACGC158250.024.0096384
CAACGCA159650.023.7419435
TATCAAC160150.023.6951332
AACGCAG163400.023.2552076
GAGTACT93100.022.3951612-13
GTACATG95200.022.2547721
GTACTTT98450.021.13043814-15
TACATGG98150.021.049332
ACGCAGA181950.020.8067
ACATGGG96600.020.6987613
CGCAGAG183250.020.6843198
AGTACTT97550.020.59455512-13
AGAGTAC149850.020.15963610-11
GTATAGG6350.019.4502371
GCAGAGT191550.019.4160969
ACTTTTT117350.017.91025516-17
TACTTTT111600.017.85327114-15
Produced by FastQC (version 0.11.5)