FastQCFastQC Report
Thu 26 May 2016
SRR937016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065329
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108200.5238874774914796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80340.3889937148028232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.24848341353847256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71450.037.2557561
GTATCAA118000.036.8995481
ATCAACG140600.030.7000923
TCAACGC141900.030.3183334
TATCAAC142950.030.2286592
CAACGCA142700.030.1483635
AACGCAG146100.029.476446
ACGCAGA161650.026.5469347
CGCAGAG161950.026.2336588
GTACATG93750.025.5544661
GCAGAGT169750.024.383739
TACATGG96700.024.1867622
GAGTACT86000.024.14881512-13
ACATGGG98450.023.0808243
GTACTTT91900.022.36710414-15
AGTACTT89900.021.80605312-13
AGAGTAC140900.021.2816310-11
CATGGGA59950.019.982534
TAACGCG1101.3443423E-619.43503476-77
CAGAGTA168150.019.33061810-11
Produced by FastQC (version 0.11.5)