##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937016_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065329 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.712821540781153 33.0 31.0 34.0 30.0 34.0 2 31.79992921224657 33.0 31.0 34.0 30.0 34.0 3 31.813838860539896 33.0 31.0 34.0 30.0 34.0 4 35.41107687927686 37.0 35.0 37.0 33.0 37.0 5 35.414356744131325 37.0 35.0 37.0 33.0 37.0 6 35.48824763512254 37.0 35.0 37.0 33.0 37.0 7 35.42676687346181 37.0 35.0 37.0 33.0 37.0 8 35.38752518363902 37.0 35.0 37.0 33.0 37.0 9 36.99982375689297 39.0 37.0 39.0 33.0 39.0 10-11 36.872876427920204 39.0 37.0 39.0 33.0 39.0 12-13 36.78622727904368 39.0 37.0 39.0 33.0 39.0 14-15 37.91283713151755 40.0 38.0 41.0 33.0 41.0 16-17 37.843805756855204 40.0 38.0 41.0 33.0 41.0 18-19 37.81503745892301 40.0 38.0 41.0 32.5 41.0 20-21 37.75936085727746 40.0 37.5 41.0 32.0 41.0 22-23 37.648438093882376 40.0 37.0 41.0 32.0 41.0 24-25 37.598739474437245 40.0 37.0 41.0 32.0 41.0 26-27 37.619971442806445 40.0 37.0 41.0 32.0 41.0 28-29 37.542997507903095 40.0 37.0 41.0 32.0 41.0 30-31 37.37572464241775 40.0 37.0 41.0 32.0 41.0 32-33 37.17804742004785 40.0 36.0 41.0 31.0 41.0 34-35 37.000649049134545 39.0 36.0 41.0 31.0 41.0 36-37 36.820625672713646 39.0 36.0 41.0 30.0 41.0 38-39 36.66145563249245 39.0 35.5 41.0 30.0 41.0 40-41 36.47891038183263 39.0 35.0 41.0 30.0 41.0 42-43 36.248962756054844 39.0 35.0 41.0 30.0 41.0 44-45 35.98221155079893 39.0 35.0 40.0 29.0 41.0 46-47 35.62492997483694 39.0 35.0 40.0 27.5 41.0 48-49 35.37119630819109 38.5 35.0 40.0 26.0 41.0 50-51 34.6759588908111 38.0 34.0 39.5 25.0 40.5 52-53 34.86171985189769 38.5 34.5 40.0 26.0 40.5 54-55 35.25294444613909 39.0 35.0 40.5 26.0 41.0 56-57 35.13810293662657 39.0 35.0 41.0 26.0 41.0 58-59 34.924779054571935 38.5 34.5 40.0 26.0 41.0 60-61 34.6436015763106 38.0 34.0 40.0 25.5 41.0 62-63 34.34046125338868 38.0 34.0 40.0 24.5 41.0 64-65 34.007009779071524 37.0 34.0 40.0 23.0 41.0 66-67 33.64390249689033 37.0 33.5 39.5 22.5 41.0 68-69 33.25836803724734 36.0 33.0 39.0 22.0 41.0 70-71 32.84038063669275 36.0 32.5 39.0 21.0 40.5 72-73 32.44597083563926 35.0 32.0 38.5 20.0 40.0 74-75 32.04468367993671 35.0 32.0 37.5 19.0 39.5 76-77 31.58832345839331 35.0 31.5 37.0 18.0 39.0 78-79 31.127713792814607 35.0 31.0 36.5 14.0 39.0 80-81 30.690225140885545 35.0 31.0 36.0 9.5 37.5 82-83 30.28893193287849 34.5 30.5 35.5 7.0 37.0 84-85 29.9199021076061 34.0 30.0 35.0 4.5 37.0 86-87 29.573193907605038 34.0 30.0 35.0 2.0 36.0 88-89 29.210983092766334 34.0 29.0 35.0 2.0 36.0 90-91 28.842787517146178 34.0 29.0 35.0 2.0 36.0 92-93 28.46771918662838 34.0 29.0 35.0 2.0 35.0 94-95 28.102212770943517 34.0 29.0 35.0 2.0 35.0 96-97 27.623652696495327 34.0 27.5 35.0 2.0 35.0 98-99 27.067135066616505 34.0 26.5 35.0 2.0 35.0 100-101 24.915742963953928 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18035738576838156 1101 2 0.12588506591215776 1101 3 0.15909604371368502 1101 4 0.10054117967674614 1101 5 0.12021812779152441 1101 6 0.17192149042546134 1101 7 0.04377055439628208 1101 8 0.1409075343873596 1101 9 0.04289181277419374 1101 10-11 0.09969461687701653 1101 12-13 0.20024253004050507 1101 14-15 0.39306659864715954 1101 16-17 0.4055683269584165 1101 18-19 0.38606215740878724 1101 20-21 0.4108736244775031 1101 22-23 0.34361597956608847 1101 24-25 0.3783275599211251 1101 26-27 0.34484180603234904 1101 28-29 0.25160508031942896 1101 30-31 0.3915469970164551 1101 32-33 0.24272662600982642 1101 34-35 0.3584338234656883 1101 36-37 0.3026359832591936 1101 38-39 0.22215653755355902 1101 40-41 0.19872608747547105 1101 42-43 0.1381813327292818 1101 44-45 0.10413991840928105 1101 46-47 0.13419898390600338 1101 48-49 0.21314626933049396 1101 50-51 0.3564682108009052 1101 52-53 0.33188590915366945 1101 54-55 0.41596019161071496 1101 56-57 0.2310896318693878 1101 58-59 0.18788522189827006 1101 60-61 0.22608413347047218 1101 62-63 0.12147362656997984 1101 64-65 0.08413219971705388 1101 66-67 0.13391439475225297 1101 68-69 0.10232967241535817 1101 70-71 -0.10958187875378655 1101 72-73 -0.10523493151106322 1101 74-75 -0.15422383570823683 1101 76-77 -0.12145095232282088 1101 78-79 -0.04502373115085945 1101 80-81 0.07471712239707173 1101 82-83 -0.00622227126562791 1101 84-85 0.046332174905163725 1101 86-87 0.08050933899898283 1101 88-89 0.11277233204629056 1101 90-91 0.11566119103144601 1101 92-93 0.14582537497424397 1101 94-95 0.20631931855847796 1101 96-97 0.19189673304600774 1101 98-99 0.0802949513408251 1101 100-101 0.462020011212374 1102 1 0.0695659953984844 1102 2 0.08400200063761787 1102 3 0.14807325116947112 1102 4 0.12360381975136647 1102 5 0.1329935353259657 1102 6 -0.055837239560368346 1102 7 -0.09533482985228403 1102 8 0.015355072778653778 1102 9 0.13749391692544322 1102 10-11 0.11955062063618982 1102 12-13 -0.06798482417897844 1102 14-15 -0.09770074590839783 1102 16-17 0.023420760892591375 1102 18-19 -0.003045759977979401 1102 20-21 -0.15525101922077766 1102 22-23 -0.09998916055128149 1102 24-25 -6.332391319858743E-4 1102 26-27 -0.07536172845537692 1102 28-29 -0.16245519823711874 1102 30-31 -0.10151837884742321 1102 32-33 -0.17843863022660145 1102 34-35 -0.15394174403642324 1102 36-37 -0.06315906749539835 1102 38-39 0.12254918980624296 1102 40-41 -0.00934960459371581 1102 42-43 -0.009083447973779357 1102 44-45 0.1347213084562 1102 46-47 0.2342274658786181 1102 48-49 0.17405891785946892 1102 50-51 0.1804848465527229 1102 52-53 0.3266456378292304 1102 54-55 0.16387391482357572 1102 56-57 0.4042361188183463 1102 58-59 0.12916755344932795 1102 60-61 0.05756822314609167 1102 62-63 -0.0761045824886466 1102 64-65 0.14724327972685813 1102 66-67 -0.18216987705601184 1102 68-69 0.16865087180563165 1102 70-71 0.06966013030345408 1102 72-73 0.24084858352215832 1102 74-75 0.4519856617868072 1102 76-77 0.17288743630997416 1102 78-79 0.08291589656648313 1102 80-81 0.11656337800718575 1102 82-83 0.21891268167182787 1102 84-85 0.08493308468174732 1102 86-87 0.21382317772294002 1102 88-89 0.14147389606914373 1102 90-91 -0.01154738883428763 1102 92-93 0.15046307605000564 1102 94-95 0.0871011273331419 1102 96-97 -0.14480950018382543 1102 98-99 -0.17558628310807833 1102 100-101 -0.08586746235591391 1103 1 0.21268732861845052 1103 2 0.24356273101768622 1103 3 0.23894363315229228 1103 4 0.10994506411834237 1103 5 0.1351424544229758 1103 6 0.042920705129702696 1103 7 0.20136613095036893 1103 8 0.17853209854176555 1103 9 0.11195399685061602 1103 10-11 0.13284402680913843 1103 12-13 0.12406610155012032 1103 14-15 0.27206246778516885 1103 16-17 0.3951133518712453 1103 18-19 0.3918800674389118 1103 20-21 0.28168102843673637 1103 22-23 0.4213950754830691 1103 24-25 0.37560263845070097 1103 26-27 0.3945158728327769 1103 28-29 0.4209341553467638 1103 30-31 0.4738482327289759 1103 32-33 0.34047982908808194 1103 34-35 0.37617567263733065 1103 36-37 0.2579302983110807 1103 38-39 0.3146937778514811 1103 40-41 0.3871073691967837 1103 42-43 0.4283776921291036 1103 44-45 0.389836865169066 1103 46-47 0.31247635255917316 1103 48-49 0.28182305887639814 1103 50-51 0.3015918819876404 1103 52-53 0.28377453577583367 1103 54-55 0.2938811952419016 1103 56-57 0.34642720588576736 1103 58-59 0.3106220162926263 1103 60-61 0.46410385999669757 1103 62-63 0.4557459191693738 1103 64-65 0.4368312186776251 1103 66-67 0.35116859331215977 1103 68-69 0.48313638005323156 1103 70-71 0.4291493850436936 1103 72-73 0.43211666499907864 1103 74-75 0.33356394488348684 1103 76-77 0.33542975042017176 1103 78-79 0.5446618152808789 1103 80-81 0.45282703543427516 1103 82-83 0.48327423681169535 1103 84-85 0.5151234383931644 1103 86-87 0.476409134486822 1103 88-89 0.3096130317718888 1103 90-91 0.29753759012158554 1103 92-93 0.23772214468787567 1103 94-95 0.557651968035465 1103 96-97 0.626118381161298 1103 98-99 0.6407781177719478 1103 100-101 0.7861277044028654 1104 1 0.23260072848415092 1104 2 0.1996830623674022 1104 3 0.20840945185931403 1104 4 0.23847730472315476 1104 5 0.2430494562406551 1104 6 0.11964572510099458 1104 7 0.10292215475382704 1104 8 0.13269944056598604 1104 9 0.2406819705464045 1104 10-11 0.34427266853002436 1104 12-13 0.23435663313370725 1104 14-15 -0.10329887865589171 1104 16-17 0.3136523870554413 1104 18-19 0.22316510764198227 1104 20-21 0.21117605153396823 1104 22-23 0.0833969510474688 1104 24-25 0.08298320508533408 1104 26-27 0.10317376974041537 1104 28-29 0.13177939668096172 1104 30-31 0.28307241694529495 1104 32-33 0.011538249446061855 1104 34-35 -0.005936023301266857 1104 36-37 -0.11941696113620992 1104 38-39 0.05603231722675872 1104 40-41 0.05796453903376886 1104 42-43 0.1311628727100711 1104 44-45 0.082177912532849 1104 46-47 -0.07621365760013532 1104 48-49 -0.1789664654596379 1104 50-51 -0.06833470544029296 1104 52-53 0.05444038646585625 1104 54-55 0.242600210802145 1104 56-57 0.10546168095928721 1104 58-59 0.28666207898510265 1104 60-61 0.2133097118734213 1104 62-63 -0.012772529669398125 1104 64-65 0.11968065832380859 1104 66-67 -0.059548896904800586 1104 68-69 -0.098774662332211 1104 70-71 0.09940063252557252 1104 72-73 0.1820035124589623 1104 74-75 0.1603323806975041 1104 76-77 0.34377183325181093 1104 78-79 0.34210592461933587 1104 80-81 0.20987740649501063 1104 82-83 0.22737908896628056 1104 84-85 0.261924414381312 1104 86-87 0.27297244491588657 1104 88-89 0.2339489864573281 1104 90-91 0.2457669111556946 1104 92-93 0.2101354468679304 1104 94-95 0.23313428186833107 1104 96-97 -0.011950330198597925 1104 98-99 -0.09989697325660174 1104 100-101 -0.08205266165057523 1105 1 0.09864443669112433 1105 2 0.12003187317914055 1105 3 0.16027867950928965 1105 4 0.16677628251130017 1105 5 0.1175815230687931 1105 6 0.16222412103155648 1105 7 0.07564033960367311 1105 8 0.11602039222972138 1105 9 0.17856291237787048 1105 10-11 0.23380511502790569 1105 12-13 0.14033641200152402 1105 14-15 0.2254574446066755 1105 16-17 0.1861922641207343 1105 18-19 0.14418401597796304 1105 20-21 0.17622617968228838 1105 22-23 0.24503948454843538 1105 24-25 0.12191295522350032 1105 26-27 0.32595712470578775 1105 28-29 0.2742467489972569 1105 30-31 0.33608762095710887 1105 32-33 0.012925511800197853 1105 34-35 -0.010157500709738088 1105 36-37 -0.03079930344019033 1105 38-39 0.1772382618372461 1105 40-41 0.1253944723528022 1105 42-43 0.29476696797664204 1105 44-45 0.1343831870458203 1105 46-47 0.16880258991635344 1105 48-49 0.22269594003391546 1105 50-51 -0.05833989947893059 1105 52-53 0.14062316730796454 1105 54-55 -0.06636592500533567 1105 56-57 -0.026316402467848832 1105 58-59 0.0022645874670033095 1105 60-61 0.11133707314523633 1105 62-63 -0.004657480343645659 1105 64-65 -0.11105345073612227 1105 66-67 -0.16002849415740172 1105 68-69 -0.1061696112372772 1105 70-71 -0.24802272115596935 1105 72-73 -0.2582477232290543 1105 74-75 -0.02144899059132399 1105 76-77 0.04597728390022482 1105 78-79 -0.022690451061134098 1105 80-81 -0.14343689507705903 1105 82-83 -0.11144738708791024 1105 84-85 -0.05839805439574519 1105 86-87 0.047441572677495714 1105 88-89 0.1444106296366563 1105 90-91 0.28376950386947897 1105 92-93 0.2660082528073744 1105 94-95 0.29709141128326166 1105 96-97 0.020007978932415682 1105 98-99 0.17174058148238203 1105 100-101 0.3084611528482668 1106 1 0.43204117398271435 1106 2 0.40037728232770675 1106 3 0.2958019291696381 1106 4 0.2972032853967974 1106 5 0.2296993858941505 1106 6 0.35888519773921246 1106 7 0.367921598116574 1106 8 0.47840828217491094 1106 9 0.49839257623423094 1106 10-11 0.5341606182022929 1106 12-13 0.4958514068569073 1106 14-15 0.45185110987888777 1106 16-17 0.5705370460255779 1106 18-19 0.4906251236053478 1106 20-21 0.4545121169301538 1106 22-23 0.4545656726243834 1106 24-25 0.5082416231244196 1106 26-27 0.4248874702884464 1106 28-29 0.3719098106753549 1106 30-31 0.5326005623050492 1106 32-33 0.5018516887714242 1106 34-35 0.576321341309864 1106 36-37 0.40540359038298135 1106 38-39 0.45617963840731335 1106 40-41 0.5349184698473053 1106 42-43 0.670677360615592 1106 44-45 0.3728528121397332 1106 46-47 0.6042803145225264 1106 48-49 0.5121702178360792 1106 50-51 0.586242922718732 1106 52-53 0.6083367077907695 1106 54-55 0.6227191016546954 1106 56-57 0.607038605331482 1106 58-59 0.6374709619288979 1106 60-61 0.734469006010201 1106 62-63 0.8186826445151567 1106 64-65 0.552645231097749 1106 66-67 0.5572049619641675 1106 68-69 0.6879343845568044 1106 70-71 0.8384214185831738 1106 72-73 0.7856403593137955 1106 74-75 0.9424760368668572 1106 76-77 0.7627741419557559 1106 78-79 0.7266438073771617 1106 80-81 0.8291826803058377 1106 82-83 0.7865914569097257 1106 84-85 0.7550136395787241 1106 86-87 1.0414554292980824 1106 88-89 1.162146856735319 1106 90-91 1.1529813803779447 1106 92-93 1.2786319664372172 1106 94-95 1.2205099849848295 1106 96-97 1.2407846463336156 1106 98-99 1.1728938900845591 1106 100-101 1.4915360698664415 1107 1 0.269739750926707 1107 2 0.30305119497080923 1107 3 0.27646766658329014 1107 4 0.12024674089975917 1107 5 0.3094003609312992 1107 6 0.2964039176964093 1107 7 0.19252444422858872 1107 8 0.23635620652544276 1107 9 0.32066227350647125 1107 10-11 0.35731627228365426 1107 12-13 0.45069269069944085 1107 14-15 0.2810557940120191 1107 16-17 0.3285644544662034 1107 18-19 0.4885643531718742 1107 20-21 0.5193953674029359 1107 22-23 0.5217011338695912 1107 24-25 0.513245122363017 1107 26-27 0.5622042328045254 1107 28-29 0.43824231297990934 1107 30-31 0.46595521256050887 1107 32-33 0.5044695748241921 1107 34-35 0.3251602193046921 1107 36-37 0.26377311967238626 1107 38-39 0.4825615710920417 1107 40-41 0.5149035035747218 1107 42-43 0.6632027058624956 1107 44-45 0.6675317158021059 1107 46-47 0.6404243716812772 1107 48-49 0.6554685216262115 1107 50-51 0.43878721328930226 1107 52-53 0.5330457669414699 1107 54-55 0.5557098941013763 1107 56-57 0.7858515152924923 1107 58-59 0.7162352121176738 1107 60-61 0.7755820304476799 1107 62-63 0.7497091136864853 1107 64-65 0.7258503931047429 1107 66-67 0.6719184363591353 1107 68-69 0.6281274533502881 1107 70-71 0.6619352357290538 1107 72-73 0.6373568521655528 1107 74-75 0.7077832671080344 1107 76-77 0.8041515479246151 1107 78-79 0.7533733617912901 1107 80-81 0.6710326046505557 1107 82-83 0.8366637680453479 1107 84-85 0.7888396934941397 1107 86-87 0.7632154348425821 1107 88-89 0.648796679883219 1107 90-91 0.7842497186661106 1107 92-93 0.6386173491191798 1107 94-95 0.6009064215773705 1107 96-97 0.5269330654241422 1107 98-99 0.752535615548446 1107 100-101 0.8526966296670722 1108 1 0.10948293013951016 1108 2 0.19727924590130996 1108 3 0.11416559315181019 1108 4 0.18337578989159198 1108 5 0.18957147415073905 1108 6 0.20425913338799262 1108 7 0.1856105662447689 1108 8 0.06920289080083109 1108 9 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58-59 -1.9509077838060875 2316 60-61 -1.9870045919698356 2316 62-63 -2.1196051095623787 2316 64-65 -2.6768049973560437 2316 66-67 -2.2342359502530726 2316 68-69 -2.254294696867852 2316 70-71 -1.9239340500370972 2316 72-73 -2.1781275857283084 2316 74-75 -2.5427613428236917 2316 76-77 -2.630102012646798 2316 78-79 -2.835769225920348 2316 80-81 -2.765223991998571 2316 82-83 -3.2325110154354064 2316 84-85 -3.1589832405697855 2316 86-87 -3.3370151166951807 2316 88-89 -2.932659302877667 2316 90-91 -3.2556512997053986 2316 92-93 -3.440117055901826 2316 94-95 -3.611691387422532 2316 96-97 -3.700893214801706 2316 98-99 -4.016414602197397 2316 100-101 -4.325732057832806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4163.0 3 1611.0 4 1491.0 5 1718.0 6 1877.0 7 2241.0 8 2827.0 9 3561.0 10 4317.0 11 5566.0 12 7283.0 13 8954.0 14 10038.0 15 12314.0 16 15866.0 17 20900.0 18 18703.0 19 11841.0 20 11498.0 21 12083.0 22 13203.0 23 14623.0 24 16448.0 25 18906.0 26 21919.0 27 26102.0 28 31222.0 29 37823.0 30 45717.0 31 57226.0 32 72464.0 33 94774.0 34 128330.0 35 184271.0 36 276622.0 37 414688.0 38 383702.0 39 68392.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.041991343668755 18.797393801074875 14.705882352941178 22.4547325023152 2 15.212520813294935 20.298616902671753 40.91692795282417 23.571934331209142 3 19.09071625539136 25.263284319945296 30.048651784754355 25.59734763990899 4 12.562779991009284 16.927345723985056 37.995070608113345 32.51480367689231 5 13.57516016787338 37.2361470539773 34.27907495178002 14.9096178263693 6 30.860146934153974 36.30210057578373 18.148796399206827 14.688956090855465 7 27.858889618782424 32.99679105215122 21.28655361713919 17.857765711927172 8 25.97046719171806 34.426417776864625 20.295531603632554 19.307583427784756 9 26.412333572356054 16.913021570185467 20.812612647531928 35.86203220992655 10-11 24.38480308078136 26.838201805133217 27.801430501208397 20.97556461287703 12-13 25.73358766568829 24.384171057081367 28.225077351080397 21.65716392614995 14-15 22.744599222615562 25.395462722027652 26.981649638268667 24.878288417088118 16-17 21.565634497652017 28.796977802924538 27.43210132508141 22.205286374342037 18-19 21.4693707887378 27.092162813186132 30.217071974916042 21.221394423160028 20-21 22.424589763663562 26.11427029478107 30.232426886079484 21.22871305547589 22-23 22.07363002585396 26.177328721144356 30.215520706900946 21.53352054610074 24-25 21.93986613514564 26.35391988688432 30.28217181138832 21.424042166581724 26-27 21.61660624683153 26.525526190025428 30.19615827656516 21.66170928657789 28-29 21.39408300126066 26.53187812910753 30.448741771622938 21.625297098008872 30-31 21.64666490738342 26.265897101298403 30.391626134556116 21.695811856762063 32-33 21.46853261980091 26.470111145651455 30.284236337277708 21.777119897269927 34-35 21.975120933182904 26.41480929115481 30.035638367414137 21.574431408248152 36-37 21.87491526277256 26.204382318875385 30.10374741914616 21.81695499920589 38-39 21.653855083649535 26.420533847297676 30.227620967595502 21.69799010145728 40-41 21.827091878276192 26.44186339857935 30.086333732571465 21.644710990572992 42-43 21.74906386354717 26.39697802297568 29.80270623145975 22.051251882017393 44-45 21.778322873911225 26.59441829521668 29.64639712814176 21.98086170273033 46-47 21.996301262843478 26.807540504415943 29.216343682841156 21.97981454989942 48-49 22.04260257848538 26.901871428218666 28.948090877882905 22.10743511541305 50-51 21.89631734972165 27.069724990527977 28.86213454026488 22.171823119485495 52-53 22.125210178638223 27.206467146763703 28.573400009442153 22.094922665155924 54-55 22.00290351666423 27.03558502761634 28.63741003989188 22.324101415827556 56-57 22.032245794955717 27.37713653873764 28.530843851240224 22.05977381506642 58-59 22.195725378534313 27.02437567047438 28.62019851973298 22.159700431258326 60-61 22.05338133948194 27.041031990165067 28.93645495961586 21.96913171073713 62-63 22.003795849261348 27.066017706699824 28.941328973360537 21.988857470678287 64-65 21.92605378918648 27.165669629131088 28.732499388704223 22.17577719297821 66-67 21.90852849867148 27.36907381950991 28.396729227570706 22.325668454247904 68-69 21.820007997405565 27.23462939581805 28.69380472331326 22.251557883463125 70-71 21.914161216771667 27.138236498838403 28.62078395646511 22.32681832792482 72-73 22.03021797244997 27.038350116522803 28.595175137670253 22.336256773356975 74-75 21.827772507579642 27.487190513062266 28.401686915365136 22.283350063992955 76-77 21.933764212373713 27.394153591944697 28.27362820749993 22.398453988181657 78-79 22.03286978753122 27.331113795736194 28.209024941103312 22.42699147562928 80-81 21.795366575550617 27.640094260657804 28.218527350441637 22.346011813349943 82-83 22.03002622821086 27.484021762319767 28.295568277498397 22.190383731970982 84-85 21.904408410642013 27.419715674510364 28.12258788687026 22.553288027977363 86-87 22.00260709718399 27.316958941972835 28.302187893586073 22.378246067257102 88-89 22.07222535601516 27.22252591353428 28.157948976791715 22.547299753658848 90-91 21.948788742968897 27.303265338249812 28.344177429022942 22.403768489758356 92-93 22.035490605427974 27.1338684130221 28.352910404022303 22.477730577527623 94-95 21.754900873617675 27.18790600511067 28.384608717000848 22.672584404270804 96-97 21.78184091551833 27.218990070779114 28.35070043040829 22.64846858329427 98-99 21.727096200149084 27.24291272660167 28.133480837031822 22.89651023621742 100-101 21.615412979529697 27.240833619614897 28.34565434275002 22.798099058105386 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 435.0 1 332.5 2 327.0 3 444.5 4 370.5 5 370.0 6 710.5 7 1572.0 8 2809.0 9 3428.0 10 3086.5 11 2617.0 12 2532.5 13 2760.5 14 3018.0 15 3131.5 16 3249.0 17 3362.5 18 3522.5 19 3835.5 20 4198.5 21 4734.5 22 5546.0 23 6689.0 24 8211.5 25 10096.5 26 12593.0 27 15489.0 28 17859.0 29 20405.5 30 24131.0 31 28369.0 32 32633.5 33 37174.5 34 42929.5 35 47320.0 36 51813.0 37 57185.5 38 59791.5 39 60939.0 40 62485.0 41 66286.0 42 69376.0 43 71929.5 44 75879.5 45 78615.5 46 78855.5 47 79687.0 48 84707.0 49 84586.0 50 78374.5 51 73554.5 52 69399.0 53 66393.0 54 63562.5 55 58401.5 56 52389.5 57 46735.5 58 42121.0 59 37627.0 60 32034.5 61 25815.0 62 19863.5 63 15218.5 64 11554.0 65 8716.0 66 6329.5 67 4810.0 68 3428.5 69 2102.5 70 1426.5 71 1005.0 72 683.5 73 482.0 74 346.5 75 269.5 76 185.0 77 90.0 78 50.0 79 32.0 80 21.5 81 14.5 82 12.5 83 10.5 84 7.5 85 10.0 86 9.0 87 7.0 88 3.5 89 2.5 90 4.0 91 2.0 92 1.0 93 1.5 94 1.5 95 2.5 96 2.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0345223448661206 2 0.02629121074656871 3 0.021836714634811207 4 0.04730481196942472 5 0.0904940568790735 6 0.10787627540212721 7 0.05330869803309787 8 0.08027776688362967 9 0.06628483888039145 10-11 0.06957729252821221 12-13 0.06599432826440726 14-15 0.05945783940476311 16-17 0.04856369130535619 18-19 0.059118910352781565 20-21 0.05582645670496081 22-23 0.031714075578273486 24-25 0.009030038313508404 26-27 0.0028082692878471176 28-29 0.006948045565621749 30-31 0.0045513329837522255 32-33 0.005374446395707415 34-35 0.006730162603633611 36-37 0.005858630755681056 38-39 0.0036555919178009894 40-41 0.0070448824376164775 42-43 0.0028324785058458 44-45 0.0022272480558787485 46-47 0.001428343861922241 48-49 3.8734748797891283E-4 50-51 0.0017672729139037897 52-53 0.005882839973679738 54-55 0.011959353691348932 56-57 0.007771158977576937 58-59 0.004527123765753544 60-61 0.0018399005678998359 62-63 0.008642690825529492 64-65 0.0021304111838840204 66-67 0.0210378104408547 68-69 0.04403656753960265 70-71 0.05546331843498057 72-73 0.0032924536478207587 74-75 0.012491956487319938 76-77 0.01951262970693773 78-79 0.023458732240722906 80-81 0.07153823918610545 82-83 0.02747746242850413 84-85 0.011184658715391108 86-87 0.028106902096469863 88-89 0.044327078155586834 90-91 0.04074411389178189 92-93 0.040623067801788475 94-95 0.011523587767372657 96-97 0.015154970467174964 98-99 0.03621699012602835 100-101 0.0731118383560198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2065329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.68528810904673 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09417672167133 46.202556600465144 2 11.732229935003382 13.535541279245052 3 3.4014915434437 5.886480590521076 4 1.4755564247492758 3.4047158993126763 5 0.7665440057522623 2.210915591004101 6 0.47985293260729717 1.6608272800453752 7 0.3177461617105288 1.2830495218670923 8 0.24389018685468267 1.125510055654529 9 0.18288099015159118 0.9494588345906047 >10 1.1459767010834219 12.596383999151225 >50 0.11002382266167539 4.357922993532766 >100 0.04736816290572207 4.662742875809984 >500 0.0015920672851012598 0.5785364819472186 >1k 4.1896507502664515E-4 0.38596489333683026 >5k 1.6758603001065805E-4 0.6363949637661499 >10k+ 8.379301500532903E-5 0.5229981397500943 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10820 0.5238874774914796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8034 0.3889937148028232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5132 0.24848341353847256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.8418435997364104E-5 0.0 3 0.0 0.0 0.0 4.8418435997364104E-5 0.0 4 0.0 0.0 0.0 4.8418435997364104E-5 0.0 5 0.0 0.0 0.0 4.8418435997364104E-5 0.0 6 0.0 0.0 0.0 4.8418435997364104E-5 0.0 7 0.0 0.0 0.0 4.8418435997364104E-5 0.0 8 0.0 0.0 0.0 4.8418435997364104E-5 0.0 9 0.0 0.0 0.0 4.8418435997364104E-5 0.0 10-11 0.0 0.0 0.0 4.8418435997364104E-5 0.0 12-13 0.0 0.0 0.0 4.8418435997364104E-5 7.262765399604616E-5 14-15 0.0 0.0 0.0 9.683687199472821E-5 9.683687199472821E-5 16-17 0.0 0.0 0.0 1.452553079920923E-4 9.683687199472821E-5 18-19 2.4209217998682052E-5 0.0 0.0 1.452553079920923E-4 9.683687199472821E-5 20-21 4.8418435997364104E-5 0.0 0.0 1.6946452599077435E-4 9.683687199472821E-5 22-23 4.8418435997364104E-5 0.0 0.0 2.6630139798550256E-4 9.683687199472821E-5 24-25 4.8418435997364104E-5 0.0 0.0 7.020673219617795E-4 9.683687199472821E-5 26-27 4.8418435997364104E-5 0.0 0.0 9.441595019486001E-4 1.2104608999341026E-4 28-29 4.8418435997364104E-5 0.0 0.0 0.0015251807339169692 1.452553079920923E-4 30-31 4.8418435997364104E-5 4.8418435997364104E-5 0.0 0.0030745706858326203 1.452553079920923E-4 32-33 4.8418435997364104E-5 4.8418435997364104E-5 0.0 0.004672379073745635 1.452553079920923E-4 34-35 4.8418435997364104E-5 4.8418435997364104E-5 0.0 0.007020673219617794 1.452553079920923E-4 36-37 9.683687199472821E-5 4.8418435997364104E-5 0.0 0.009490013455483363 1.452553079920923E-4 38-39 9.683687199472821E-5 4.8418435997364104E-5 0.0 0.01169305229336343 1.9367374398945641E-4 40-41 9.683687199472821E-5 4.8418435997364104E-5 0.0 0.014210810965226365 1.9367374398945641E-4 42-43 1.452553079920923E-4 4.8418435997364104E-5 0.0 0.01854426098699045 1.9367374398945641E-4 44-45 1.9367374398945641E-4 4.8418435997364104E-5 0.0 0.024766030012651735 2.4209217998682052E-4 46-47 2.1788296198813845E-4 7.262765399604616E-5 0.0 0.03875895801588996 2.905106159841846E-4 48-49 2.420921799868205E-4 9.683687199472821E-5 0.0 0.06984359392619771 2.905106159841846E-4 50-51 2.420921799868205E-4 9.683687199472821E-5 0.0 0.09151084403501815 2.905106159841846E-4 52-53 2.420921799868205E-4 9.683687199472821E-5 0.0 0.11279074665585967 2.905106159841846E-4 54-55 2.420921799868205E-4 9.683687199472821E-5 0.0 0.14293122306421882 2.905106159841846E-4 56-57 2.420921799868205E-4 9.683687199472821E-5 0.0 0.16379956897908277 2.905106159841846E-4 58-59 2.420921799868205E-4 9.683687199472821E-5 0.0 0.18832350681174767 2.905106159841846E-4 60-61 2.420921799868205E-4 9.683687199472821E-5 0.0 0.2311496134514162 2.905106159841846E-4 62-63 2.420921799868205E-4 9.683687199472821E-5 0.0 0.2611932529877806 2.905106159841846E-4 64-65 2.420921799868205E-4 9.683687199472821E-5 0.0 0.2935851866700172 2.905106159841846E-4 66-67 2.420921799868205E-4 9.683687199472821E-5 0.0 0.3639855926101846 2.905106159841846E-4 68-69 2.420921799868205E-4 9.683687199472821E-5 0.0 0.4418908561299435 2.905106159841846E-4 70-71 2.420921799868205E-4 1.2104608999341026E-4 0.0 0.5078125567403546 2.905106159841846E-4 72-73 2.6630139798550256E-4 1.452553079920923E-4 0.0 0.5847010331041689 2.905106159841846E-4 74-75 2.905106159841846E-4 1.452553079920923E-4 0.0 0.6508890351125656 2.905106159841846E-4 76-77 2.905106159841846E-4 1.452553079920923E-4 0.0 0.6968381308740641 2.905106159841846E-4 78-79 2.905106159841846E-4 1.452553079920923E-4 0.0 0.7641881753463976 2.905106159841846E-4 80-81 3.8734748797891283E-4 1.452553079920923E-4 0.0 0.8720644507485248 2.905106159841846E-4 82-83 3.8734748797891283E-4 1.452553079920923E-4 0.0 0.9736705386889934 2.905106159841846E-4 84-85 4.1155670597759484E-4 1.452553079920923E-4 0.0 1.1023667415699872 2.905106159841846E-4 86-87 4.357659239762769E-4 1.452553079920923E-4 0.0 1.3520121975723964 2.905106159841846E-4 88-89 4.357659239762769E-4 1.452553079920923E-4 0.0 1.6642869005373961 2.905106159841846E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7145 0.0 37.255756 1 GTATCAA 11800 0.0 36.899548 1 ATCAACG 14060 0.0 30.700092 3 TCAACGC 14190 0.0 30.318333 4 TATCAAC 14295 0.0 30.228659 2 CAACGCA 14270 0.0 30.148363 5 AACGCAG 14610 0.0 29.47644 6 ACGCAGA 16165 0.0 26.546934 7 CGCAGAG 16195 0.0 26.233658 8 GTACATG 9375 0.0 25.554466 1 GCAGAGT 16975 0.0 24.38373 9 TACATGG 9670 0.0 24.186762 2 GAGTACT 8600 0.0 24.148815 12-13 ACATGGG 9845 0.0 23.080824 3 GTACTTT 9190 0.0 22.367104 14-15 AGTACTT 8990 0.0 21.806053 12-13 AGAGTAC 14090 0.0 21.28163 10-11 CATGGGA 5995 0.0 19.98253 4 TAACGCG 110 1.3443423E-6 19.435034 76-77 CAGAGTA 16815 0.0 19.330618 10-11 >>END_MODULE