FastQCFastQC Report
Thu 26 May 2016
SRR937016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065329
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105450.5105724075922045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85510.4140260462134604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.26731818514144723No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28320.13712101074453514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.11591373577768967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82000.034.93881
GTATCAA129750.034.054941
ATCAACG158150.027.7504863
TCAACGC159950.027.4381944
TATCAAC160750.027.4198322
CAACGCA162450.026.9867025
AACGCAG165100.026.6430726
ACGCAGA185000.023.5717247
CGCAGAG185950.023.428598
GAGTACT96250.022.95085712-13
GTACATG98000.022.6894681
GCAGAGT194500.021.9101729
TACATGG101600.021.364382
GTACTTT102850.021.22197714-15
AGAGTAC157000.020.95526110-11
AGTACTT102200.020.73149512-13
ACATGGG99250.020.625983
GTATAGG7550.020.1375921
TACTTTT115700.018.2286414-15
ACTTTTT121350.018.18171116-17
Produced by FastQC (version 0.11.5)