##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937016_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065329 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.171152876854 33.0 31.0 34.0 31.0 34.0 2 32.30329211471877 33.0 31.0 34.0 31.0 34.0 3 32.42688210933948 34.0 31.0 34.0 31.0 34.0 4 36.085437235423505 37.0 35.0 37.0 35.0 37.0 5 35.92840414287506 37.0 35.0 37.0 35.0 37.0 6 36.02090804903238 37.0 35.0 37.0 35.0 37.0 7 35.97950302348924 37.0 35.0 37.0 35.0 37.0 8 35.93106764103927 37.0 35.0 37.0 35.0 37.0 9 37.633902395211614 39.0 37.0 39.0 35.0 39.0 10-11 37.54749437014635 39.0 37.0 39.0 35.0 39.0 12-13 37.44336907098094 39.0 37.0 39.0 34.5 39.0 14-15 38.65332641918067 40.0 38.0 41.0 34.0 41.0 16-17 38.65303736111777 40.0 38.0 41.0 34.5 41.0 18-19 38.783548044887766 40.0 38.0 41.0 35.0 41.0 20-21 38.786949440016585 40.0 38.0 41.0 35.0 41.0 22-23 38.69257561386104 40.0 38.0 41.0 34.0 41.0 24-25 38.5962977327099 40.0 38.0 41.0 34.0 41.0 26-27 38.406236488230206 40.0 38.0 41.0 34.0 41.0 28-29 38.25485697436099 40.0 38.0 41.0 34.0 41.0 30-31 38.237926984030146 40.0 38.0 41.0 34.0 41.0 32-33 38.10471067805662 40.0 37.0 41.0 34.0 41.0 34-35 37.98189707305713 40.0 37.0 41.0 33.0 41.0 36-37 37.83674659097896 40.0 37.0 41.0 33.0 41.0 38-39 37.67827038694561 40.0 37.0 41.0 33.0 41.0 40-41 37.35171950812679 40.0 36.5 41.0 32.5 41.0 42-43 37.15272191500725 40.0 36.0 41.0 32.0 41.0 44-45 36.92103994085204 39.5 36.0 41.0 31.0 41.0 46-47 36.53782956613692 39.0 36.0 41.0 30.5 41.0 48-49 36.1597832597131 39.0 35.5 40.5 30.0 41.0 50-51 35.87444760616832 39.0 35.0 40.0 29.0 41.0 52-53 35.62043238631715 39.0 35.0 40.0 28.0 41.0 54-55 35.226350135983175 38.0 35.0 40.0 27.0 41.0 56-57 35.03245148835852 38.0 34.0 40.0 27.0 41.0 58-59 34.71979016418207 38.0 34.0 40.0 26.0 41.0 60-61 34.34001677214623 37.5 34.0 40.0 26.0 41.0 62-63 33.97602803233771 37.0 33.0 39.5 25.5 41.0 64-65 33.65327243262453 36.5 33.0 39.0 25.0 40.0 66-67 33.32148098438554 36.0 32.5 39.0 23.5 40.0 68-69 32.87590161180131 35.5 32.0 39.0 22.5 40.0 70-71 32.42878035412276 35.0 32.0 38.0 22.0 40.0 72-73 32.012185467787454 35.0 31.0 37.5 21.0 39.5 74-75 31.518223246756328 35.0 31.0 37.0 20.0 39.0 76-77 30.08138461233053 33.5 29.5 35.5 18.5 37.5 78-79 30.7575265248297 34.0 30.5 36.0 18.0 38.0 80-81 30.614380081817473 34.0 30.5 35.5 18.0 37.0 82-83 30.263725779282623 34.0 30.0 35.0 15.0 37.0 84-85 29.821151012744217 34.0 30.0 35.0 9.5 36.0 86-87 29.441144001754683 34.0 29.0 35.0 7.0 36.0 88-89 29.071346502179555 34.0 29.0 35.0 2.0 35.5 90-91 28.615992657828365 33.5 29.0 35.0 2.0 35.0 92-93 28.129740104361098 33.0 28.5 35.0 2.0 35.0 94-95 27.575448996261613 33.0 27.0 34.5 2.0 35.0 96-97 26.815128727674864 32.5 25.5 34.0 2.0 35.0 98-99 25.952072284851468 32.0 24.5 34.0 2.0 35.0 100-101 22.737193202632607 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08530091263954631 1101 2 0.13153268503693027 1101 3 0.0740633797133512 1101 4 0.06777375528390905 1101 5 -0.00930318560247656 1101 6 0.033209336219755414 1101 7 0.04709172991059063 1101 8 0.15260830057449226 1101 9 0.1270092254391173 1101 10-11 0.13822394450216535 1101 12-13 0.08671448078256105 1101 14-15 0.2544935909118564 1101 16-17 0.2796724417329344 1101 18-19 0.05883080028610976 1101 20-21 -0.06675351920654293 1101 22-23 2.9146360810017313E-4 1101 24-25 0.03947008054947787 1101 26-27 0.047108647885394817 1101 28-29 0.1241423755626343 1101 30-31 0.19307816203076555 1101 32-33 0.14622462075065812 1101 34-35 0.03392094692771508 1101 36-37 0.12041496829176168 1101 38-39 0.08433158480818292 1101 40-41 0.29079364323537504 1101 42-43 0.2675732849050476 1101 44-45 0.05909578296038376 1101 46-47 -0.1714011791542447 1101 48-49 -0.018413736655013224 1101 50-51 0.07459412930572284 1101 52-53 0.04407691457114993 1101 54-55 -0.14306878450289418 1101 56-57 -0.1276293264093482 1101 58-59 0.026215027652092715 1101 60-61 -0.013633200307914706 1101 62-63 0.01311019078787723 1101 64-65 0.09299362595154292 1101 66-67 0.02517544793093407 1101 68-69 -0.21628044280808467 1101 70-71 -0.29541000796040606 1101 72-73 -0.2968845056653464 1101 74-75 -0.4064119762000047 1101 76-77 -0.3620732919651104 1101 78-79 -0.2712750715344612 1101 80-81 -0.2315061226499644 1101 82-83 0.041302757179742855 1101 84-85 -0.14275588852293808 1101 86-87 -0.4903915116974211 1101 88-89 -0.5275530971682194 1101 90-91 -0.1806965421065918 1101 92-93 -0.44168864811859976 1101 94-95 -0.49492819385170606 1101 96-97 -0.7171871217737156 1101 98-99 -0.6036550972803596 1101 100-101 -0.24199370802629616 1102 1 -0.1963112271719467 1102 2 -0.05134548558132224 1102 3 -0.011885049392660108 1102 4 0.0692806558690009 1102 5 -0.11264714967244771 1102 6 0.05127646944826125 1102 7 -0.011969495406354724 1102 8 0.07665484318916072 1102 9 0.12627588193101502 1102 10-11 0.16748525290604022 1102 12-13 0.207978497744449 1102 14-15 0.03826626012390477 1102 16-17 0.007680210867789583 1102 18-19 0.10194694973873197 1102 20-21 0.0813483053495645 1102 22-23 -0.061183129965712624 1102 24-25 -0.06985731426353681 1102 26-27 0.009507219448771309 1102 28-29 -0.07063758655428387 1102 30-31 -0.07274326502530215 1102 32-33 -0.18994640787443728 1102 34-35 -0.01970543038702033 1102 36-37 0.03810214662279776 1102 38-39 0.06716996932462393 1102 40-41 0.1441786916404766 1102 42-43 0.09805900148868574 1102 44-45 0.06072403669723769 1102 46-47 0.04145612790453157 1102 48-49 0.10071714046308244 1102 50-51 0.15864333804834274 1102 52-53 0.1358921709025651 1102 54-55 0.29239358287940576 1102 56-57 0.08943845213611468 1102 58-59 0.04635300135685583 1102 60-61 0.08497466609944127 1102 62-63 0.16669111410408988 1102 64-65 0.23796536246211275 1102 66-67 0.0947871146788799 1102 68-69 0.019065669363072857 1102 70-71 0.13769369111796692 1102 72-73 -0.004479534761603787 1102 74-75 0.12914022259561264 1102 76-77 0.13690909892471126 1102 78-79 -0.1518811438466905 1102 80-81 0.01512820745213972 1102 82-83 0.19549163366329125 1102 84-85 0.08738114158784072 1102 86-87 -0.1705078492355554 1102 88-89 -0.1750991353717417 1102 90-91 0.02227068098498819 1102 92-93 -0.03835961373188468 1102 94-95 -0.1772434536661862 1102 96-97 -0.10274809156033982 1102 98-99 -0.2527727894141414 1102 100-101 -0.25772615860318737 1103 1 0.012574337405894198 1103 2 0.14366012087221236 1103 3 0.06971475610856714 1103 4 0.04558076852949 1103 5 -0.10589682019366364 1103 6 -0.08918445543057629 1103 7 0.004004911531836797 1103 8 0.10073203688017429 1103 9 0.05445947069897272 1103 10-11 0.08055765586922092 1103 12-13 0.05574800607708141 1103 14-15 0.15844597220196022 1103 16-17 0.08451685581719914 1103 18-19 0.07708691179552574 1103 20-21 0.0783222154124843 1103 22-23 0.11484124349317426 1103 24-25 0.13012435183627957 1103 26-27 0.1674620728838505 1103 28-29 0.173855932810568 1103 30-31 0.21586221601372557 1103 32-33 0.13199887207625238 1103 34-35 0.17597308334480743 1103 36-37 0.16165137713040423 1103 38-39 0.13132134888418534 1103 40-41 0.1618204206601206 1103 42-43 0.006866779845118742 1103 44-45 -0.009233410054740432 1103 46-47 0.02293888872419103 1103 48-49 -0.0553123213173734 1103 50-51 -0.07948977935239299 1103 52-53 -0.09066368637997613 1103 54-55 0.12843271645648002 1103 56-57 0.11849303401690747 1103 58-59 -0.05504575565785785 1103 60-61 -0.20168848435545073 1103 62-63 -0.03364859446661228 1103 64-65 -0.024103126611954906 1103 66-67 -0.05210163574625426 1103 68-69 -0.012487570688797689 1103 70-71 -0.14052291195574895 1103 72-73 5.709233201933728E-4 1103 74-75 -0.054121723667456934 1103 76-77 0.13106085047645166 1103 78-79 0.05378635625959305 1103 80-81 -0.031698964982179945 1103 82-83 0.26555071399234365 1103 84-85 0.03432741794614458 1103 86-87 -0.14695509808769103 1103 88-89 0.27152829054867667 1103 90-91 -0.1315733267524486 1103 92-93 -0.15090750772473882 1103 94-95 -0.018648574404220852 1103 96-97 0.010278742361091275 1103 98-99 -0.018938174479188774 1103 100-101 0.4584806770187839 1104 1 -0.027011866197042878 1104 2 0.07901375014722589 1104 3 -0.08381144245812777 1104 4 -0.039231718445762453 1104 5 -0.17186393703438085 1104 6 -0.03140357663603055 1104 7 0.020621601739534867 1104 8 -0.03379376081358032 1104 9 -0.10592100403879101 1104 10-11 0.14071039642193028 1104 12-13 0.11345399254290811 1104 14-15 0.14366604349077505 1104 16-17 0.08016595522790482 1104 18-19 0.08289622290187992 1104 20-21 0.08832278343643196 1104 22-23 -0.006414411420337274 1104 24-25 0.044810411877499234 1104 26-27 0.04620460897688616 1104 28-29 -0.01001513588487768 1104 30-31 0.033492702329048996 1104 32-33 0.04995577316917377 1104 34-35 0.015117386028485669 1104 36-37 -0.05799371282721921 1104 38-39 0.0020155826813947897 1104 40-41 0.09303633922358046 1104 42-43 -0.06434886013334307 1104 44-45 -0.05452924922509794 1104 46-47 -0.09173175869285188 1104 48-49 -0.04460163291577146 1104 50-51 -0.023913430422155102 1104 52-53 0.10175406230597162 1104 54-55 0.13256952267131084 1104 56-57 -0.18227763337142733 1104 58-59 -0.3213694793768056 1104 60-61 -0.15436612027598784 1104 62-63 0.04206194197954005 1104 64-65 0.13035162829114455 1104 66-67 -0.20224112723096255 1104 68-69 -0.062006685960753316 1104 70-71 -0.13887736106659077 1104 72-73 0.034404708660684236 1104 74-75 -0.18578388473444463 1104 76-77 0.005617285283221918 1104 78-79 0.29417255103464157 1104 80-81 0.04182612648053308 1104 82-83 0.28669956593274293 1104 84-85 0.007971559267609507 1104 86-87 -0.03965456132315737 1104 88-89 -0.09008760008392258 1104 90-91 -0.019136062645198848 1104 92-93 -0.004248157833568911 1104 94-95 0.0038563894737961846 1104 96-97 -0.024058576305403534 1104 98-99 -0.2893062648478164 1104 100-101 0.4519931742691128 1105 1 -0.06477804599923331 1105 2 -0.02948940385152099 1105 3 -0.036606359737092475 1105 4 -0.03126824345777379 1105 5 -0.017218579886488783 1105 6 0.03411959261212871 1105 7 0.048020644815217395 1105 8 0.002677040626188898 1105 9 0.035792292856683616 1105 10-11 0.031374317463154 1105 12-13 0.05959206629434277 1105 14-15 0.06705113585619671 1105 16-17 -0.18346330783703735 1105 18-19 -0.07664403618161941 1105 20-21 0.03573245726489915 1105 22-23 -0.010079007256024397 1105 24-25 -0.05769464516690448 1105 26-27 -0.028331117554436958 1105 28-29 -0.07538242526210581 1105 30-31 -0.03955634317338763 1105 32-33 0.0486835859739827 1105 34-35 -0.050895244057322486 1105 36-37 -0.059059430136876756 1105 38-39 -0.16000485920721985 1105 40-41 -0.12498715098250557 1105 42-43 -0.1061803439140121 1105 44-45 -0.0686144095396628 1105 46-47 -0.22206836918927308 1105 48-49 -0.330410654338543 1105 50-51 -0.19069608554269024 1105 52-53 -0.21227909891041463 1105 54-55 -0.07170424739442893 1105 56-57 -0.25465883196923755 1105 58-59 -0.3572098137679873 1105 60-61 -0.36083140671919267 1105 62-63 -0.37238975620102366 1105 64-65 -0.29383996871721507 1105 66-67 -0.31207175538476406 1105 68-69 -0.3198228921441313 1105 70-71 -0.4045053700471506 1105 72-73 -0.4587979308659875 1105 74-75 -0.4465231565200547 1105 76-77 -0.6334608296134547 1105 78-79 -0.429726034871706 1105 80-81 -0.4556284227458036 1105 82-83 -0.4772125902312929 1105 84-85 -0.7930183806584594 1105 86-87 -0.5049070495299084 1105 88-89 -0.39154283115014366 1105 90-91 -0.45208922392420803 1105 92-93 -0.3958628188785127 1105 94-95 -0.24518034533510757 1105 96-97 -0.22870927622853188 1105 98-99 -0.2762041360356591 1105 100-101 0.1452161085134449 1106 1 -0.0160209081739211 1106 2 0.06276509052757717 1106 3 0.04445729986129976 1106 4 0.05877354676346869 1106 5 -0.011187323542479533 1106 6 0.0542859553446533 1106 7 0.1646422129765739 1106 8 0.169150603561377 1106 9 0.24884976330335462 1106 10-11 0.10141588211957497 1106 12-13 0.24663551441703646 1106 14-15 0.30315645120450085 1106 16-17 0.26259861230953874 1106 18-19 0.254119557135418 1106 20-21 0.12120395714950405 1106 22-23 0.24387640204869854 1106 24-25 0.19242106524950486 1106 26-27 0.2732547983515232 1106 28-29 0.20467159317521322 1106 30-31 0.22257969497136543 1106 32-33 0.16837814951472296 1106 34-35 0.1745713281034611 1106 36-37 0.17955182363916578 1106 38-39 0.3367542731316675 1106 40-41 0.23864470727255593 1106 42-43 0.22891778112177263 1106 44-45 0.015236060068943402 1106 46-47 0.06956970424422337 1106 48-49 0.07379511023078322 1106 50-51 0.16781968057087937 1106 52-53 0.3091819842171617 1106 54-55 0.47908243161467823 1106 56-57 0.42603496638622573 1106 58-59 0.3255377089229583 1106 60-61 0.2356048209000079 1106 62-63 0.23118711629064848 1106 64-65 0.2271558869629402 1106 66-67 0.3080731786895754 1106 68-69 0.19251843167846516 1106 70-71 0.19464284079002425 1106 72-73 0.24600419239304117 1106 74-75 0.28025223383722775 1106 76-77 0.2833204991715661 1106 78-79 0.22789400031641094 1106 80-81 0.24473831529188672 1106 82-83 0.30035251558463827 1106 84-85 0.5633038759961337 1106 86-87 0.5629384029965045 1106 88-89 0.6332660460402195 1106 90-91 0.37837007377104115 1106 92-93 0.5128625923868881 1106 94-95 0.8053722017255254 1106 96-97 0.9641099326982818 1106 98-99 0.8945883425920762 1106 100-101 1.0840958494956254 1107 1 0.13719339935001784 1107 2 0.21462332192902522 1107 3 0.11039419918694193 1107 4 0.0986488730601991 1107 5 -0.062206658532055314 1107 6 0.026077100235426087 1107 7 0.0597624714355689 1107 8 0.11259670413976863 1107 9 0.10294896138643139 1107 10-11 0.12752870784682813 1107 12-13 0.1540046831200783 1107 14-15 0.22253062546658242 1107 16-17 0.18516257034581685 1107 18-19 0.15208633957097817 1107 20-21 0.15498845154830576 1107 22-23 0.10665475387823875 1107 24-25 0.10993790472944909 1107 26-27 0.2257533613373397 1107 28-29 0.27029661437904906 1107 30-31 0.2338521383219856 1107 32-33 0.10302877816602063 1107 34-35 0.1804462443786079 1107 36-37 0.1508673968018499 1107 38-39 0.13220584454182216 1107 40-41 0.13136663024472028 1107 42-43 0.15074651132466244 1107 44-45 0.14791293882566237 1107 46-47 0.07796727106174473 1107 48-49 0.06134954956261396 1107 50-51 -0.13645922910126274 1107 52-53 -0.10448944891005851 1107 54-55 0.2363738852332915 1107 56-57 -0.03178212424405302 1107 58-59 -0.07373583557309615 1107 60-61 -0.08584652950560923 1107 62-63 -0.11980517472192531 1107 64-65 0.027109335457737416 1107 66-67 -0.037762097318925214 1107 68-69 -0.13191696471935188 1107 70-71 -0.14957100824642566 1107 72-73 -0.0019133486793911914 1107 74-75 0.00821151375014395 1107 76-77 -0.3013414462693902 1107 78-79 -0.07245904959475524 1107 80-81 0.01643819887677367 1107 82-83 0.015006691940445904 1107 84-85 0.06313606278762407 1107 86-87 0.27576965536527354 1107 88-89 0.19409282112835413 1107 90-91 0.0454615037382915 1107 92-93 0.151541368099263 1107 94-95 0.2550725854785867 1107 96-97 0.13786397112011883 1107 98-99 0.13094991474860151 1107 100-101 0.5575658534601367 1108 1 0.09466623376552263 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-0.9936482764558363 2316 52-53 -0.9345301818078298 2316 54-55 -0.7773561727210492 2316 56-57 -0.7254976690796795 2316 58-59 -0.7736214184234811 2316 60-61 -0.7979019364518862 2316 62-63 -0.7660780802306348 2316 64-65 -1.112262969704382 2316 66-67 -1.1288179340654736 2316 68-69 -1.031917749742675 2316 70-71 -0.8965682940282775 2316 72-73 -1.2722236193358079 2316 74-75 -1.0356367000274105 2316 76-77 -0.9667806962581054 2316 78-79 -1.1684440968629701 2316 80-81 -1.096429020315476 2316 82-83 -1.021184109941256 2316 84-85 -1.0113467049691138 2316 86-87 -0.9036572383563062 2316 88-89 -1.4324237458076432 2316 90-91 -1.419429064010206 2316 92-93 -1.2274740262081423 2316 94-95 -1.4857416603396914 2316 96-97 -1.2379387152296992 2316 98-99 -1.3445276738167458 2316 100-101 -1.909404780779834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 11.0 5 38.0 6 92.0 7 257.0 8 575.0 9 1074.0 10 1773.0 11 3109.0 12 4588.0 13 6455.0 14 8540.0 15 11815.0 16 15224.0 17 21736.0 18 19998.0 19 12041.0 20 10527.0 21 10610.0 22 11176.0 23 12175.0 24 14133.0 25 16942.0 26 20893.0 27 25782.0 28 32309.0 29 40243.0 30 50683.0 31 63323.0 32 81179.0 33 107069.0 34 147293.0 35 210651.0 36 323399.0 37 470205.0 38 294433.0 39 14970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93835300316683 19.758605642963584 15.069664094550806 22.23337725931877 2 15.552571831699252 21.208514684940873 39.817913221409796 23.42100026195008 3 19.363257290381558 25.480360466444974 30.045164826438853 25.111217416734615 4 13.105708582022524 17.628087341048328 37.20337050416665 32.0628335727625 5 13.55350019997269 37.793531343419446 33.93100796888754 14.72196048772032 6 31.081972896327898 36.91663652619026 17.50273201025115 14.498658567230693 7 27.893229239798927 33.42607109206216 20.87398478300236 17.806714885136554 8 26.3923084409312 34.86630943544588 19.657691341185835 19.083690782437085 9 26.512883183688015 16.983646181139267 20.465369181069544 36.03810145410317 10-11 24.600560007630744 27.25846584248805 27.279866791198888 20.86110735868232 12-13 25.897934884135328 24.712680689769716 27.886981039709745 21.502403386385215 14-15 22.746209425087667 25.84723235011287 26.694392069934253 24.712166154865212 16-17 21.65577493948906 29.232001293740613 27.111322215492063 22.00090155127827 18-19 21.470533123454537 27.43527755276546 30.032479244126453 21.061710079653555 20-21 22.440201442011826 26.42323739450126 30.15265512327695 20.983906040209963 22-23 22.103054996719614 26.566778272458546 30.05362404100121 21.276542689820634 24-25 22.007200338736936 26.692722360874434 30.210434753557504 21.089642546831133 26-27 21.84415215303522 26.772036506541212 30.12128579061394 21.26252554980963 28-29 21.471490955185306 26.883273205647342 30.324471621200903 21.320764217966452 30-31 21.757816793353506 26.608109410171455 30.252468250821057 21.381605545653986 32-33 21.6317371611704 26.831907691108402 30.12809354840506 21.408261599316138 34-35 21.998650094004393 26.71138593415383 30.01030344318024 21.279660528661534 36-37 22.071372612818852 26.469300857695387 29.980458063760167 21.478868465725593 38-39 21.73615033681581 26.75239734459902 30.07586133594686 21.435590982638303 40-41 21.993460587036505 26.742620887870807 29.844508582918994 21.419409942173694 42-43 21.87329476320722 26.880850460144607 29.57446489154997 21.67138988509821 44-45 21.92538510534609 26.98220099002015 29.37191314724195 21.720500757391804 46-47 22.199411714863277 27.27231791606648 28.869378911309358 21.658891457760884 48-49 22.329673543379066 27.417921027555913 28.55936199964364 21.69304342942138 50-51 22.17918294560056 27.456099534352685 28.500041398404203 21.864676121642553 52-53 22.39810257419597 27.75086917334298 28.117688043694827 21.733340208766226 54-55 22.239410767001285 27.49762870709703 28.223324225825525 22.03963630007616 56-57 22.226093019371067 27.890486836801514 28.102342370259365 21.78107777356805 58-59 22.433708980426807 27.48211540556571 28.17447617203104 21.909699441976443 60-61 22.29343912008355 27.548229374416245 28.505505530224127 21.652825975276073 62-63 22.13247865110111 27.631602519501737 28.504441665226217 21.731477164170936 64-65 22.119803730932812 27.647085596089337 28.34704705499562 21.88606361798223 66-67 22.153527187933346 27.834731335652258 28.009304091166147 22.002437385248253 68-69 22.039251421022293 27.670031832739276 28.2592122843531 22.031504461885323 70-71 22.193041832864154 27.664025905988144 28.161020551838607 21.98191170930909 72-73 22.269613398237027 27.542122478564423 28.102181917396194 22.086082205802356 74-75 22.109841668423915 28.005434681294727 27.859133099576834 22.02559055070453 76-77 22.210340873134708 28.000315688279127 27.733287949108338 22.056055489477824 78-79 22.25452011841253 27.862652893834472 27.698489630188416 22.184337357564583 80-81 22.11794270275374 28.167599347788986 27.621088156220758 22.09336979323652 82-83 22.279818711472497 28.166708750408226 27.66916029983637 21.884312238282906 84-85 22.186372804196246 28.033416381212277 27.53311968941659 22.247091125174887 86-87 22.17388420276403 27.889665363718986 27.671151692452057 22.26529874106493 88-89 22.19161015876121 27.93347024834265 27.477767450913838 22.397152141982303 90-91 22.128519996833372 27.94305592508944 27.634648798630895 22.293775279446297 92-93 22.24025808963124 27.847234024215993 27.583547221774353 22.32896066437841 94-95 22.008575873383855 27.75180128686519 27.651260404516663 22.588362435234288 96-97 22.01412420693263 27.80974081734085 27.659594556677554 22.516540419048965 98-99 21.912722902696792 27.82327276283865 27.400260590798364 22.863743743666195 100-101 21.74111983707869 28.193114628128768 27.30963159437784 22.756133940414706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 650.0 1 483.5 2 379.0 3 470.0 4 437.5 5 419.5 6 701.0 7 1445.0 8 2378.0 9 3023.5 10 2939.0 11 2595.0 12 2716.0 13 2930.0 14 3191.0 15 3442.0 16 3565.5 17 3726.0 18 3972.5 19 4235.5 20 4606.5 21 5205.0 22 5902.5 23 6936.5 24 8220.0 25 10030.0 26 12661.0 27 15604.5 28 18180.5 29 20846.5 30 24423.0 31 28461.0 32 32549.0 33 37017.0 34 42738.0 35 47207.5 36 52066.5 37 57220.5 38 59371.0 39 60852.0 40 62540.0 41 66026.0 42 69191.5 43 72103.0 44 76350.5 45 78320.5 46 77959.0 47 79874.5 48 85447.0 49 84510.0 50 78329.0 51 73903.0 52 69484.0 53 65798.5 54 62167.0 55 57442.0 56 51782.0 57 46180.5 58 41999.0 59 37791.5 60 32152.5 61 25708.5 62 20049.5 63 15484.5 64 11270.0 65 8458.0 66 6337.0 67 4883.5 68 3457.5 69 2039.0 70 1416.0 71 1035.0 72 752.5 73 524.0 74 397.0 75 288.0 76 179.5 77 94.5 78 52.0 79 30.0 80 19.5 81 13.5 82 7.0 83 3.5 84 1.0 85 1.0 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023821870510703138 2 0.002420921799868205 3 2.420921799868205E-4 4 0.0 5 0.0022756664918761127 6 0.0 7 3.389290519815487E-4 8 0.0 9 4.8418435997364104E-5 10-11 0.0 12-13 0.005713375447688964 14-15 0.003631382699802308 16-17 0.0 18-19 4.84184359973641E-4 20-21 9.68368719947282E-4 22-23 0.0011620424639367385 24-25 4.5997514197495897E-4 26-27 2.4209217998682052E-5 28-29 9.683687199472821E-5 30-31 0.0 32-33 1.2104608999341025E-4 34-35 0.0 36-37 0.0013072977719288308 38-39 0.008691109261526856 40-41 2.905106159841846E-4 42-43 0.0 44-45 8.473226299538718E-4 46-47 1.9367374398945641E-4 48-49 4.8418435997364104E-5 50-51 0.0015493899519156513 52-53 2.1788296198813845E-4 54-55 0.0 56-57 3.1471983398286663E-4 58-59 1.9367374398945641E-4 60-61 0.0020335743118892924 62-63 0.0 64-65 4.8418435997364104E-5 66-67 4.8418435997364104E-5 68-69 1.2104608999341025E-4 70-71 7.262765399604615E-4 72-73 6.052304499670513E-4 74-75 0.003607173481803625 76-77 2.4209217998682052E-5 78-79 3.389290519815487E-4 80-81 0.002251457273877431 82-83 1.2104608999341025E-4 84-85 0.0026388047618563434 86-87 5.810212319683692E-4 88-89 0.0027840600698484357 90-91 0.0019125282218958818 92-93 0.0 94-95 0.0 96-97 4.5997514197495897E-4 98-99 0.0010652055919420102 100-101 0.001815691349901154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2065329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.97603122261183 #Duplication Level Percentage of deduplicated Percentage of total 1 79.03487988304913 44.24058904008933 2 12.308135594303035 13.779211646376934 3 3.604252051110049 6.052551760412965 4 1.5378809238679276 3.4433788244436094 5 0.8242746978473008 2.306981311135472 6 0.5113289670430955 1.7173299734538099 7 0.33816315446864775 1.3250321905011753 8 0.24721087763169228 1.1070307043904704 9 0.19339067874036706 0.9742718404199593 >10 1.2310504300193157 13.147690860104147 >50 0.11342951530065391 4.366451294894699 >100 0.05323031225933358 5.177518306438504 >500 0.0022529929170736736 0.8452036586893857 >1k 2.599607212008077E-4 0.32270530509385764 >5k 1.7330714746720513E-4 0.6825656175080062 >10k+ 8.665357373360256E-5 0.511487666047607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10545 0.5105724075922045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8551 0.4140260462134604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5521 0.26731818514144723 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2832 0.13712101074453514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2394 0.11591373577768967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.8418435997364104E-5 0.0 3 0.0 0.0 0.0 4.8418435997364104E-5 0.0 4 0.0 0.0 0.0 4.8418435997364104E-5 0.0 5 0.0 0.0 0.0 4.8418435997364104E-5 0.0 6 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 7 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 8 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 9 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 10-11 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 12-13 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 14-15 0.0 0.0 0.0 4.8418435997364104E-5 4.8418435997364104E-5 16-17 0.0 0.0 0.0 9.683687199472821E-5 9.683687199472821E-5 18-19 0.0 0.0 0.0 9.683687199472821E-5 1.452553079920923E-4 20-21 0.0 0.0 0.0 1.2104608999341026E-4 1.452553079920923E-4 22-23 0.0 0.0 0.0 1.9367374398945641E-4 1.452553079920923E-4 24-25 9.683687199472821E-5 2.4209217998682052E-5 0.0 6.294396679657333E-4 1.452553079920923E-4 26-27 9.683687199472821E-5 4.8418435997364104E-5 0.0 8.715318479525538E-4 1.6946452599077435E-4 28-29 9.683687199472821E-5 4.8418435997364104E-5 0.0 0.0014767622979196052 1.9367374398945641E-4 30-31 9.683687199472821E-5 9.683687199472821E-5 0.0 0.0029535245958392103 1.9367374398945641E-4 32-33 9.683687199472821E-5 9.683687199472821E-5 0.0 0.004527123765753543 1.9367374398945641E-4 34-35 9.683687199472821E-5 9.683687199472821E-5 0.0 0.006875417911625702 2.420921799868205E-4 36-37 9.683687199472821E-5 9.683687199472821E-5 0.0 0.009393176583488635 2.905106159841846E-4 38-39 1.452553079920923E-4 9.683687199472821E-5 0.0 0.01157200620337002 2.905106159841846E-4 40-41 1.452553079920923E-4 9.683687199472821E-5 0.0 0.014113974093231636 2.905106159841846E-4 42-43 1.452553079920923E-4 9.683687199472821E-5 0.0 0.018495842550993086 2.905106159841846E-4 44-45 1.452553079920923E-4 9.683687199472821E-5 0.0 0.02442710096067019 2.905106159841846E-4 46-47 2.905106159841846E-4 9.683687199472821E-5 0.0 0.03842002896390841 2.905106159841846E-4 48-49 2.905106159841846E-4 9.683687199472821E-5 0.0 0.06935940956622408 2.905106159841846E-4 50-51 2.905106159841846E-4 9.683687199472821E-5 0.0 0.09100245045704583 2.905106159841846E-4 52-53 2.905106159841846E-4 9.683687199472821E-5 0.0 0.11218551620589262 3.389290519815487E-4 54-55 2.905106159841846E-4 9.683687199472821E-5 0.0 0.14208390043426494 3.389290519815487E-4 56-57 2.905106159841846E-4 9.683687199472821E-5 0.0 0.16312171087511967 3.389290519815487E-4 58-59 2.905106159841846E-4 1.2104608999341026E-4 0.0 0.18762143948978588 3.389290519815487E-4 60-61 2.905106159841846E-4 1.452553079920923E-4 0.0 0.22972126958949396 3.389290519815487E-4 62-63 2.905106159841846E-4 1.452553079920923E-4 0.0 0.25952281694587154 3.389290519815487E-4 64-65 2.905106159841846E-4 1.452553079920923E-4 0.0 0.29206000593610026 3.8734748797891283E-4 66-67 2.905106159841846E-4 1.452553079920923E-4 0.0 0.36236357500427296 3.8734748797891283E-4 68-69 2.905106159841846E-4 1.452553079920923E-4 0.0 0.44046251226802124 3.8734748797891283E-4 70-71 2.905106159841846E-4 1.452553079920923E-4 0.0 0.5064568405324286 4.1155670597759484E-4 72-73 2.905106159841846E-4 1.452553079920923E-4 0.0 0.5834179445502388 5.08393577972323E-4 74-75 2.905106159841846E-4 1.452553079920923E-4 0.0 0.6499932940466143 5.326027959710051E-4 76-77 3.147198339828667E-4 1.452553079920923E-4 0.0 0.6963781557320892 5.326027959710051E-4 78-79 3.389290519815487E-4 1.452553079920923E-4 0.0 0.7638250370764174 5.326027959710051E-4 80-81 3.8734748797891283E-4 1.452553079920923E-4 0.0 0.8726212627624945 5.326027959710051E-4 82-83 4.357659239762769E-4 1.452553079920923E-4 0.0 0.9742031414849643 5.326027959710051E-4 84-85 4.357659239762769E-4 1.452553079920923E-4 0.0 1.1030203904559515 5.326027959710051E-4 86-87 4.357659239762769E-4 1.9367374398945641E-4 0.0 1.3524721727143714 5.326027959710051E-4 88-89 4.357659239762769E-4 1.9367374398945641E-4 0.0 1.6637058793054278 5.326027959710051E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8200 0.0 34.9388 1 GTATCAA 12975 0.0 34.05494 1 ATCAACG 15815 0.0 27.750486 3 TCAACGC 15995 0.0 27.438194 4 TATCAAC 16075 0.0 27.419832 2 CAACGCA 16245 0.0 26.986702 5 AACGCAG 16510 0.0 26.643072 6 ACGCAGA 18500 0.0 23.571724 7 CGCAGAG 18595 0.0 23.42859 8 GAGTACT 9625 0.0 22.950857 12-13 GTACATG 9800 0.0 22.689468 1 GCAGAGT 19450 0.0 21.910172 9 TACATGG 10160 0.0 21.36438 2 GTACTTT 10285 0.0 21.221977 14-15 AGAGTAC 15700 0.0 20.955261 10-11 AGTACTT 10220 0.0 20.731495 12-13 ACATGGG 9925 0.0 20.62598 3 GTATAGG 755 0.0 20.137592 1 TACTTTT 11570 0.0 18.22864 14-15 ACTTTTT 12135 0.0 18.181711 16-17 >>END_MODULE