FastQCFastQC Report
Thu 26 May 2016
SRR937015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424859
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107100.7516533214865471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79920.5608976046050872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.3622814608322648No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26340.1848603967129379No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21040.1476637337448828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.13966294208760305No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18350.1287846727290209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133200.028.8683621
GTGTAGC7500.025.9836651
GGTATCA92550.025.4218181
GTACATG95150.025.22671
TATCAAC157650.024.2412592
ATCAACG157950.024.225313
TACATGG96700.024.18422
GAGTACT74150.024.12395312-13
TCAACGC160400.023.8552884
ACATGGG96300.023.8404223
CAACGCA160000.023.8241255
AACGCAG162800.023.413556
GTACTTT80150.022.25915514-15
AGTACTT77000.021.93529912-13
CATGGGG35300.021.8139044
ACGCAGA177900.021.346087
CGCAGAG178850.021.1522278
GTACCTG12150.021.1249311
ATGGGGA18750.020.5327115
CATGGGA59450.020.4682734
Produced by FastQC (version 0.11.5)