##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937015_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1424859 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.669891547163616 33.0 31.0 34.0 30.0 34.0 2 31.745558683350424 33.0 31.0 34.0 30.0 34.0 3 31.73946334339047 33.0 31.0 34.0 30.0 34.0 4 35.33097380161826 37.0 35.0 37.0 33.0 37.0 5 35.35957452632155 37.0 35.0 37.0 33.0 37.0 6 35.42804867007894 37.0 35.0 37.0 33.0 37.0 7 35.37088582098299 37.0 35.0 37.0 33.0 37.0 8 35.33589218301601 37.0 35.0 37.0 33.0 37.0 9 36.92092550912055 39.0 37.0 39.0 33.0 39.0 10-11 36.79407506286587 39.0 37.0 39.0 33.0 39.0 12-13 36.6807224434137 39.0 37.0 39.0 32.0 39.0 14-15 37.76551293847321 40.0 37.5 41.0 32.0 41.0 16-17 37.70788232379485 40.0 37.0 41.0 32.0 41.0 18-19 37.70813848949265 40.0 37.5 41.0 32.0 41.0 20-21 37.6710986841505 40.0 37.0 41.0 32.0 41.0 22-23 37.5330274785084 40.0 37.0 41.0 32.0 41.0 24-25 37.3510856161908 40.0 37.0 41.0 32.0 41.0 26-27 37.28599215782053 40.0 37.0 41.0 31.5 41.0 28-29 37.332972595884925 40.0 37.0 41.0 32.0 41.0 30-31 37.16518511656241 39.5 36.5 41.0 31.0 41.0 32-33 36.94526054858761 39.0 36.0 41.0 31.0 41.0 34-35 36.72546055434257 39.0 35.0 41.0 30.0 41.0 36-37 36.54150094851491 39.0 35.0 41.0 30.0 41.0 38-39 36.359255547391 39.0 35.0 41.0 30.0 41.0 40-41 36.12841761886615 39.0 35.0 41.0 30.0 41.0 42-43 35.88850370457708 39.0 35.0 40.0 29.0 41.0 44-45 35.576955684737925 38.5 35.0 40.0 27.5 41.0 46-47 35.137446933345686 38.0 35.0 40.0 25.5 41.0 48-49 34.70626777807489 38.0 34.0 40.0 24.0 41.0 50-51 34.01848989970236 37.5 33.5 39.5 23.0 40.5 52-53 34.31100445728314 38.0 34.0 40.0 23.5 40.5 54-55 34.60805349862688 38.0 34.0 40.0 23.0 41.0 56-57 34.489135416206096 38.0 34.0 40.0 23.0 41.0 58-59 34.243089316206024 38.0 34.0 40.0 22.5 41.0 60-61 33.94241535478247 38.0 34.0 40.0 22.0 41.0 62-63 33.63275032827809 37.0 33.5 40.0 20.5 41.0 64-65 33.3021386677559 37.0 33.0 40.0 20.0 41.0 66-67 32.92983446081331 36.0 33.0 39.0 18.5 41.0 68-69 32.487712117479695 36.0 32.0 39.0 17.0 41.0 70-71 32.12282303020861 35.0 32.0 39.0 14.0 40.0 72-73 31.736093536272712 35.0 31.5 38.0 11.5 40.0 74-75 31.313792803358087 35.0 31.0 37.0 7.5 39.0 76-77 30.882260279789087 35.0 31.0 36.5 7.0 39.0 78-79 30.460642070548737 35.0 30.5 36.0 2.0 39.0 80-81 30.044709687063772 34.5 30.0 36.0 2.0 37.5 82-83 29.643632808579653 34.0 30.0 35.0 2.0 37.0 84-85 29.31022192371315 34.0 29.0 35.0 2.0 36.5 86-87 28.972333753725806 34.0 29.0 35.0 2.0 36.0 88-89 28.599059275338824 34.0 29.0 35.0 2.0 36.0 90-91 28.263461858331247 34.0 29.0 35.0 2.0 35.5 92-93 27.915128795200086 34.0 27.5 35.0 2.0 35.0 94-95 27.53462728592794 34.0 27.0 35.0 2.0 35.0 96-97 27.083864438516372 34.0 26.5 35.0 2.0 35.0 98-99 26.484396701708732 33.5 25.0 35.0 2.0 35.0 100-101 24.41948747209373 31.0 13.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4952714625901713 1101 2 0.37106975310010526 1101 3 0.5507184073624316 1101 4 0.41628101905015313 1101 5 0.3379973540672907 1101 6 0.27944276813222046 1101 7 0.36468781911569437 1101 8 0.22791041870921447 1101 9 0.4204678921015699 1101 10-11 0.4086750633321259 1101 12-13 0.6059240719253367 1101 14-15 0.827525196461643 1101 16-17 0.8024424143181008 1101 18-19 0.7752720592207609 1101 20-21 0.7056918346703753 1101 22-23 0.8018106971486674 1101 24-25 0.8676423971920997 1101 26-27 0.9165677363056446 1101 28-29 0.8270672620939692 1101 30-31 0.708857306117018 1101 32-33 0.7650056946215784 1101 34-35 0.872843546946747 1101 36-37 0.8670299159676986 1101 38-39 0.9152387930903743 1101 40-41 0.8862704930587597 1101 42-43 0.9968703454366121 1101 44-45 1.0779949235384905 1101 46-47 1.103424887215212 1101 48-49 1.0765393386646238 1101 50-51 1.1025000596552843 1101 52-53 1.1178225778802968 1101 54-55 1.1814089250652913 1101 56-57 1.2255874298918243 1101 58-59 1.1233328790889772 1101 60-61 1.2379816530030467 1101 62-63 1.2682619106088637 1101 64-65 1.11108470876664 1101 66-67 1.2831232764501337 1101 68-69 1.1528352277453067 1101 70-71 1.1633631465778578 1101 72-73 1.1673254331195437 1101 74-75 1.0549907277849897 1101 76-77 1.0402189657198804 1101 78-79 1.2773328471864183 1101 80-81 1.252760690549195 1101 82-83 1.0182145932795557 1101 84-85 1.2355158302959843 1101 86-87 1.0674667809719267 1101 88-89 1.1410476961443834 1101 90-91 0.9822702504892185 1101 92-93 0.9434956413306423 1101 94-95 1.0157801364222614 1101 96-97 1.0767757894381411 1101 98-99 1.1576610595519945 1101 100-101 1.239434278172002 1102 1 0.25832892588736556 1102 2 0.2696721173097991 1102 3 0.298058791079967 1102 4 0.16349357439403178 1102 5 0.20211134963362554 1102 6 0.1732710501940673 1102 7 0.21819000894561924 1102 8 0.1923150016640136 1102 9 0.1967860111241393 1102 10-11 0.20916641646039835 1102 12-13 0.23981831206085502 1102 14-15 0.30267419854586564 1102 16-17 0.18479980877938118 1102 18-19 0.1732375031782709 1102 20-21 0.21319303605201156 1102 22-23 0.23833194707773941 1102 24-25 0.31862893146411153 1102 26-27 0.3198062715702079 1102 28-29 0.3950282081333043 1102 30-31 0.4161715157787924 1102 32-33 0.35459766235141643 1102 34-35 0.24611692398781315 1102 36-37 0.06488602223340934 1102 38-39 -0.11659354803501998 1102 40-41 -0.1400747980378867 1102 42-43 0.012255037066040586 1102 44-45 0.11095242778176839 1102 46-47 0.12320837876178814 1102 48-49 0.12477216266682234 1102 50-51 0.04139502096156633 1102 52-53 0.2098410630935703 1102 54-55 0.45888450608625675 1102 56-57 0.5182407504559947 1102 58-59 0.19399770283962425 1102 60-61 0.12322236340069281 1102 62-63 0.20725188872765443 1102 64-65 0.45486903700803794 1102 66-67 0.2584262536208044 1102 68-69 0.20949823045431515 1102 70-71 0.4029516280793146 1102 72-73 0.3098609054932382 1102 74-75 0.5800941354706879 1102 76-77 0.5400135516755782 1102 78-79 0.559674451083886 1102 80-81 0.5234143720405378 1102 82-83 0.4276533173473176 1102 84-85 0.5625966814005388 1102 86-87 0.6781846090653225 1102 88-89 0.49775157658998737 1102 90-91 0.27996988662267697 1102 92-93 0.453945098196769 1102 94-95 0.5826712888531524 1102 96-97 0.48389753878652186 1102 98-99 0.523226624912283 1102 100-101 0.830477815695783 1103 1 0.20647878759901417 1103 2 0.3477990840491536 1103 3 0.24147487063063977 1103 4 0.26815761704059327 1103 5 0.13297703813272932 1103 6 0.10761214786001005 1103 7 0.19219486009427555 1103 8 0.1010395850129413 1103 9 0.26085723387895143 1103 10-11 0.2954586896623965 1103 12-13 0.00954404866320857 1103 14-15 0.2711522782258058 1103 16-17 0.3749442546223847 1103 18-19 0.37009939512625323 1103 20-21 0.32842040387862426 1103 22-23 0.18569902526424187 1103 24-25 0.4864618355875976 1103 26-27 0.6494879191917491 1103 28-29 0.5418428132885893 1103 30-31 0.5630590922796372 1103 32-33 0.6503438921652247 1103 34-35 0.6093970284950245 1103 36-37 0.6237148746611965 1103 38-39 0.6473933069169959 1103 40-41 0.8896651926408268 1103 42-43 0.9621727104197575 1103 44-45 1.0671172307591377 1103 46-47 0.7350176599004854 1103 48-49 0.5243001452217371 1103 50-51 0.5830604686041951 1103 52-53 0.6494975024320979 1103 54-55 0.6699341368070222 1103 56-57 0.8205780447669468 1103 58-59 0.7409885278410684 1103 60-61 0.8126939079778452 1103 62-63 0.8846243079403777 1103 64-65 0.9596870168754279 1103 66-67 0.9522073599956755 1103 68-69 0.9811120368370112 1103 70-71 0.9035328473981821 1103 72-73 0.8314192604571673 1103 74-75 0.9652962609950109 1103 76-77 0.8496886335769815 1103 78-79 0.9109828324127491 1103 80-81 0.8513615469098212 1103 82-83 0.7663798397949328 1103 84-85 0.8195168823864165 1103 86-87 0.8766431957005061 1103 88-89 0.8175261308524355 1103 90-91 0.7653654117271991 1103 92-93 0.7193209601601609 1103 94-95 0.8379968363083421 1103 96-97 0.7584878616634683 1103 98-99 0.9162312413279459 1103 100-101 1.2175174923765901 1104 1 0.3726599144276257 1104 2 0.3756996451952723 1104 3 0.42551567589283934 1104 4 0.4036296860525326 1104 5 0.41538825146599123 1104 6 0.4208784682130329 1104 7 0.3993180780450558 1104 8 0.39311586252855335 1104 9 0.5305459388540328 1104 10-11 0.5011739711667005 1104 12-13 0.47315677485968166 1104 14-15 0.5755979274532379 1104 16-17 0.5911898311940362 1104 18-19 0.6285105937070625 1104 20-21 0.5178821793722292 1104 22-23 0.6319475707653908 1104 24-25 0.5884921407972357 1104 26-27 0.7118142322054339 1104 28-29 0.7516522670239993 1104 30-31 0.6164740066324725 1104 32-33 0.6343343547096936 1104 34-35 0.6140008976924776 1104 36-37 0.7302452287383119 1104 38-39 0.6866438458422408 1104 40-41 0.7378232577101755 1104 42-43 0.8060743263656818 1104 44-45 0.6963978831122475 1104 46-47 0.7379218630563997 1104 48-49 0.7912268237272357 1104 50-51 0.7696174877252062 1104 52-53 0.8034934787027836 1104 54-55 0.8084319322385625 1104 56-57 0.6851445165169352 1104 58-59 0.9094485385734714 1104 60-61 0.8344734953888491 1104 62-63 0.8449736089511006 1104 64-65 0.9147317075627655 1104 66-67 0.7596903531929584 1104 68-69 0.8481755442676331 1104 70-71 0.7792494013008451 1104 72-73 0.8966079165647614 1104 74-75 0.9123655956413117 1104 76-77 0.9702236678671952 1104 78-79 0.9912460897894313 1104 80-81 0.9136035865828305 1104 82-83 0.8316216047036633 1104 84-85 1.0074333392917616 1104 86-87 1.034028177342936 1104 88-89 0.838689619289287 1104 90-91 0.9708033472700777 1104 92-93 0.8999240769829271 1104 94-95 1.0776233781693243 1104 96-97 0.8300836889344652 1104 98-99 0.8303816019330164 1104 100-101 1.1092159229008764 1105 1 0.40720207029211153 1105 2 0.2654791753699577 1105 3 0.32983871276156407 1105 4 0.35608091069845926 1105 5 0.3473111148508181 1105 6 0.2391736278679204 1105 7 0.3551128182206327 1105 8 0.37290694272068237 1105 9 0.4315186635664361 1105 10-11 0.496251089515944 1105 12-13 0.4400072902776131 1105 14-15 0.5081953560344985 1105 16-17 0.5182531065139102 1105 18-19 0.6772116096753109 1105 20-21 0.7566043455930824 1105 22-23 0.6985258388065105 1105 24-25 0.8249943588338695 1105 26-27 0.6956250922803164 1105 28-29 0.6854340078015113 1105 30-31 0.6094263295707876 1105 32-33 0.628891531119109 1105 34-35 0.5132691558742337 1105 36-37 0.6363545040522425 1105 38-39 0.6749258242892893 1105 40-41 0.7847613986373787 1105 42-43 0.8611588615337595 1105 44-45 0.8029649537109336 1105 46-47 0.7629623348442465 1105 48-49 0.6755711765163426 1105 50-51 0.5580809785831136 1105 52-53 0.7498487664146083 1105 54-55 0.8972483379360057 1105 56-57 1.0851289692334447 1105 58-59 1.207036925517322 1105 60-61 0.974913254125596 1105 62-63 0.8227104087339541 1105 64-65 0.8160926895733667 1105 66-67 0.882479697271755 1105 68-69 0.9250942425946889 1105 70-71 0.8765728472415404 1105 72-73 0.6884168345035704 1105 74-75 0.8619140235178406 1105 76-77 0.5773726996240072 1105 78-79 0.757376502612285 1105 80-81 0.8618186012656679 1105 82-83 0.7909193687409264 1105 84-85 0.8909495656007387 1105 86-87 0.8479582035226905 1105 88-89 0.740863647764602 1105 90-91 0.6802396987729225 1105 92-93 0.6135080699076383 1105 94-95 0.6275029716800766 1105 96-97 0.7797062425205574 1105 98-99 0.7742969006723683 1105 100-101 1.0252828934617106 1106 1 0.5083737177122423 1106 2 0.536453590501555 1106 3 0.5169625782401255 1106 4 0.4627943671865964 1106 5 0.5019269073577846 1106 6 0.4014569767391123 1106 7 0.44788930801040294 1106 8 0.35333865629494454 1106 9 0.43045291092050775 1106 10-11 0.6041561893726666 1106 12-13 0.6561674190892077 1106 14-15 0.8785902027331005 1106 16-17 0.71432489720344 1106 18-19 0.6429020692523508 1106 20-21 0.784481157987635 1106 22-23 0.770220997954894 1106 24-25 0.7911865492120498 1106 26-27 0.7667002199900352 1106 28-29 0.7826216456989599 1106 30-31 0.7317167346039071 1106 32-33 0.8298416527436814 1106 34-35 0.7929302925967932 1106 36-37 0.7159158704141362 1106 38-39 0.8242921568700723 1106 40-41 0.9516943289808069 1106 42-43 0.9129588371407991 1106 44-45 1.080900523833634 1106 46-47 0.8562502393368874 1106 48-49 1.0108974742769519 1106 50-51 0.9196954053982012 1106 52-53 0.8815854250078772 1106 54-55 0.8715888773136271 1106 56-57 0.9569421149468482 1106 58-59 1.1616725569365087 1106 60-61 1.1238192087829475 1106 62-63 1.1823226234318085 1106 64-65 1.1510638236943578 1106 66-67 1.1994149569861463 1106 68-69 1.086022630403015 1106 70-71 1.0579656749774955 1106 72-73 0.9800234566173103 1106 74-75 0.9328383964328637 1106 76-77 1.0540572666776988 1106 78-79 0.9732678377187334 1106 80-81 1.0502471762088597 1106 82-83 1.2146447709761823 1106 84-85 1.156968963764566 1106 86-87 1.2848555442185834 1106 88-89 1.3016031733162308 1106 90-91 1.188088893726615 1106 92-93 1.329978607029176 1106 94-95 1.5361332649138575 1106 96-97 1.4608608986190355 1106 98-99 1.6964477528295525 1106 100-101 1.7438195041065718 1107 1 0.23354964666460631 1107 2 0.35061003946050917 1107 3 0.27240582980386563 1107 4 0.2193159605623336 1107 5 0.2654409607217332 1107 6 0.1868915401084621 1107 7 0.2580151052430324 1107 8 0.34243064220386543 1107 9 0.363520849248296 1107 10-11 0.4054328790309256 1107 12-13 0.4022522840310856 1107 14-15 0.3425808496543752 1107 16-17 0.46137747614448443 1107 18-19 0.5738194699457324 1107 20-21 0.46325430671779344 1107 22-23 0.4538746211554425 1107 24-25 0.4502673885307402 1107 26-27 0.39049860918203905 1107 28-29 0.31720149746886506 1107 30-31 0.4756770426856036 1107 32-33 0.44074380020832393 1107 34-35 0.5510660463325792 1107 36-37 0.6076224161724255 1107 38-39 0.4262390387581192 1107 40-41 0.6324679973264509 1107 42-43 0.6153300716745562 1107 44-45 0.7776755554715109 1107 46-47 0.9080251731976574 1107 48-49 0.7984163662109012 1107 50-51 0.6689638929539754 1107 52-53 0.7937528082410594 1107 54-55 1.003983271053663 1107 56-57 1.0743707446426 1107 58-59 1.0628324727923228 1107 60-61 0.9819750766665223 1107 62-63 0.857940085864449 1107 64-65 1.0130450113789067 1107 66-67 1.007719027028081 1107 68-69 1.0465310154883767 1107 70-71 1.0244739427303244 1107 72-73 0.8634432528498195 1107 74-75 1.0461838541275057 1107 76-77 1.077581879969241 1107 78-79 1.1646595411914973 1107 80-81 1.2334644341476633 1107 82-83 1.1812737236157425 1107 84-85 1.0341463901034835 1107 86-87 1.0787467099172545 1107 88-89 1.0709687675077113 1107 90-91 0.969854580666663 1107 92-93 1.2316128815370035 1107 94-95 1.2917916261136675 1107 96-97 1.3027170433515032 1107 98-99 1.3863588315345332 1107 100-101 1.6056738609147878 1108 1 0.1510226416900693 1108 2 0.3289468978612611 1108 3 0.19542476656464558 1108 4 0.22078613553386361 1108 5 0.217922558883771 1108 6 0.21337046612148214 1108 7 0.24173668900898093 1108 8 0.031179021412377494 1108 9 0.023224644981731046 1108 10-11 0.16052499914944462 1108 12-13 0.09352811192891863 1108 14-15 0.4341214273710179 1108 16-17 0.3488930677888433 1108 18-19 0.39454588034246285 1108 20-21 0.3549514171485413 1108 22-23 0.36695631890679437 1108 24-25 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58-59 -1.7740089769523522 2316 60-61 -1.8362318399274926 2316 62-63 -1.879293691691906 2316 64-65 -2.01556447368219 2316 66-67 -2.103887510384908 2316 68-69 -1.507572857970331 2316 70-71 -1.7128698526779864 2316 72-73 -1.8037894176086589 2316 74-75 -2.0376428296991804 2316 76-77 -2.456667576860859 2316 78-79 -2.5230281464961912 2316 80-81 -2.1803853603472945 2316 82-83 -2.3989604275861502 2316 84-85 -2.0528493614083985 2316 86-87 -2.0830999293487373 2316 88-89 -2.5946793691081673 2316 90-91 -2.50286929528302 2316 92-93 -2.969244427384819 2316 94-95 -2.956472997957409 2316 96-97 -3.2362341197932096 2316 98-99 -3.119930868303552 2316 100-101 -3.007158787401732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4185.0 3 1383.0 4 1330.0 5 1367.0 6 1564.0 7 1840.0 8 2345.0 9 2848.0 10 3416.0 11 4350.0 12 5711.0 13 7122.0 14 8193.0 15 10271.0 16 13245.0 17 17439.0 18 15443.0 19 9848.0 20 8822.0 21 9094.0 22 9887.0 23 10644.0 24 12092.0 25 13946.0 26 16065.0 27 19045.0 28 22462.0 29 26780.0 30 32756.0 31 41024.0 32 51547.0 33 67469.0 34 92118.0 35 131119.0 36 193368.0 37 274656.0 38 238013.0 39 42032.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.59025000649541 18.284748852419415 14.475630984991747 21.649370156093433 2 15.470932029559695 20.311191660344488 40.784231082637895 23.433645227457923 3 19.66787483294368 25.733239414265256 29.885225374160505 24.713660378630557 4 12.547755080817371 16.55029311799835 37.57299843234747 33.328953368836814 5 13.394594024974973 37.75428982910937 33.54022867379736 15.310887472118306 6 30.743573135657876 35.87733705754497 18.032874410422924 15.346215396374232 7 27.98043254666344 33.837811862434876 20.4373521129927 17.744403477908982 8 26.75275521522254 33.483495402062275 19.83087625292135 19.932873129793833 9 27.38936517326806 16.609030089525227 20.366532095446736 35.63507264175998 10-11 24.746591597266814 26.771259398311358 26.963335520593734 21.51881348382809 12-13 26.664503172539362 24.421177516800448 27.592592982833235 21.32172632782696 14-15 23.49467328098636 25.004003995566105 27.134094513964108 24.36722820948343 16-17 21.492577997938742 28.69197971340513 27.392769300050652 22.422672988605477 18-19 21.304183677948988 26.698274726721984 30.869204091487774 21.12833750384126 20-21 22.705989681729243 25.505694994388577 30.58964858043012 21.198666743452055 22-23 22.155962610101202 25.752557922633574 30.538700947178466 21.55277852008676 24-25 21.927254303060607 25.757163457841575 30.915253380317843 21.40032885877998 26-27 21.80045965776352 25.843601200194087 30.76673527125496 21.589203870787436 28-29 21.377454849864737 26.01621163046135 31.146535329331225 21.45979819034269 30-31 21.563522969999198 25.897931555539326 30.996263065578212 21.54228240888326 32-33 21.39189128936595 26.013588829256324 30.741979006231286 21.852540875146442 34-35 21.906600142391532 25.86842685442502 30.61112507565428 21.61384792752917 36-37 22.08018497960548 25.597283164707264 30.698827446693027 21.623704408994232 38-39 21.974022271639857 25.606066244752107 30.77176940798782 21.648142075620218 40-41 21.83594006489587 25.844792757730396 30.68708792684695 21.632179250526782 42-43 21.66556357080032 26.087579374623644 30.28807480218887 21.958782252387163 44-45 21.827170583948064 26.291144093323844 29.94132854749537 21.94035677523273 46-47 22.135485959045724 26.328886685964097 29.33291864678324 22.202708708206934 48-49 22.182751238719526 26.568243142194543 29.05744274366984 22.191562875416096 50-51 22.264518506818302 26.802892293652047 28.88085083978308 22.051738359746572 52-53 22.25973369467916 26.927167987193755 28.791937120209504 22.02116119791758 54-55 21.846592085963703 26.767078889701025 28.88896378321183 22.497365241123443 56-57 22.171708678261183 27.23272575475696 28.365716966008858 22.229848600973 58-59 22.351560722541645 26.774895301119876 28.49554520660298 22.377998769735495 60-61 22.16435829260954 26.523556720558183 29.14909595460797 22.162989032224313 62-63 22.326714109354132 26.541255335195647 29.081749452611383 22.050281102838838 64-65 22.08546551284117 26.825775790376433 28.899839254991296 22.188919441791104 66-67 21.998341287475526 27.00973176890946 28.67409739915 22.317829544465013 68-69 21.907659251015097 27.05997180729956 28.802793289224454 22.22957565246089 70-71 21.99331422510226 27.003749341004706 28.528617068100974 22.474319365792052 72-73 21.959396496444743 27.052471199436468 28.320071543818308 22.66806076030048 74-75 21.892761266118523 27.378359898009613 28.259894359078043 22.468984476793818 76-77 22.150843999987362 27.08305614250774 28.340727234046888 22.425372623458014 78-79 22.127227617435125 27.06722406406184 28.260939789985805 22.544608528517223 80-81 22.047860501886532 27.38670622745393 28.237120394072623 22.328312876586914 82-83 22.058172413345126 27.258430414343 28.258939183322205 22.424457988989662 84-85 22.08149400764897 27.07221720499276 28.084097973148538 22.762190814209728 86-87 22.316990738708686 26.8803581617873 28.29158583154106 22.511065267962955 88-89 22.306621660397 26.83377661769525 28.083343313523972 22.776258408383782 90-91 21.920884235837423 26.939129079105932 28.517574882511077 22.62241180254557 92-93 22.020961742677507 27.140280594901643 28.25129650127262 22.587461161148227 94-95 21.838827360282302 27.181666722857173 28.082751761572432 22.896754155288086 96-97 21.83769575399797 27.086157749589418 27.898711574475378 23.17743492193723 98-99 21.808735818584683 27.20109021194701 27.821167579333896 23.16900639013441 100-101 21.94853163322518 26.944449518788264 28.14291194577041 22.964106902216145 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 734.0 1 559.5 2 488.5 3 628.5 4 567.0 5 583.0 6 1000.5 7 2008.5 8 3344.5 9 3967.0 10 3400.5 11 2632.5 12 2461.0 13 2648.0 14 2867.5 15 2937.0 16 2930.5 17 2982.5 18 3085.5 19 3283.0 20 3550.5 21 3954.0 22 4547.5 23 5314.5 24 6160.5 25 7376.5 26 9092.0 27 11032.0 28 12685.0 29 14352.5 30 16648.5 31 19003.0 32 21318.5 33 23660.5 34 26264.0 35 28601.5 36 31277.0 37 34484.5 38 36156.5 39 36677.0 40 38214.5 41 40890.5 42 42997.0 43 44977.5 44 47942.0 45 50515.5 46 51214.0 47 53197.5 48 62421.0 49 62971.5 50 54338.5 51 50768.0 52 48727.5 53 47954.0 54 46492.0 55 42953.0 56 38651.5 57 34857.0 58 32352.0 59 29186.5 60 24696.0 61 20280.5 62 16294.5 63 12683.5 64 9392.5 65 6889.5 66 5011.0 67 3760.5 68 2718.5 69 1899.0 70 1344.5 71 935.0 72 685.5 73 524.5 74 386.5 75 304.0 76 206.0 77 96.0 78 49.5 79 32.0 80 26.5 81 23.5 82 19.0 83 14.0 84 11.0 85 12.5 86 11.0 87 6.5 88 4.5 89 4.5 90 5.0 91 2.5 92 2.5 93 2.5 94 2.0 95 1.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05446152917586933 2 0.09116691546321426 3 0.06583107521516164 4 0.12015223962511379 5 0.1006415371626245 6 0.09692187086581901 7 0.1035190148639269 8 0.09025454448475252 9 0.11039688839386914 10-11 0.10281719103434094 12-13 0.0867454253368228 14-15 0.08990363256995955 16-17 0.10218554958771359 18-19 0.08221866163599346 20-21 0.06856818815054683 22-23 0.07007710938415661 24-25 0.059198840025574455 26-27 0.053057881516697436 28-29 0.06618198712995461 30-31 0.04891712092214037 32-33 0.04733801730557199 34-35 0.043302530285452806 36-37 0.04926803283693334 38-39 0.05025058619835366 40-41 0.06214650010983543 42-43 0.0587075633448643 44-45 0.04610982560379658 46-47 0.0343893676497113 48-49 0.04253052407290827 50-51 0.026950035056100285 52-53 0.03888104015906135 54-55 0.04421490126391454 56-57 0.0497242183261642 58-59 0.05439134679291074 60-61 0.05140859551717047 62-63 0.057900465940840466 64-65 0.03884594896758206 66-67 0.06056739649326705 68-69 0.05133841313421188 70-71 0.024002374971839317 72-73 0.019475611271009975 74-75 0.02884495939598234 76-77 0.04207433858367741 78-79 0.06035684934439126 80-81 0.038810857776102756 82-83 0.058497016195988516 84-85 0.061549949854687376 86-87 0.06467306589634483 88-89 0.08460486265658566 90-91 0.06281323274794208 92-93 0.07207730729847656 94-95 0.07937627512617038 96-97 0.08849998491078766 98-99 0.11576584069020163 100-101 0.11067761792570353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1424859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.384919924928234 #Duplication Level Percentage of deduplicated Percentage of total 1 80.54845271018377 47.83363414271131 2 11.512060835127174 13.672856217298596 3 3.3607114796148307 5.987267463231414 4 1.4913659396232553 3.542585876131695 5 0.7858109596356142 2.3332660457045984 6 0.46768449430074693 1.6664043746508248 7 0.3122961048405228 1.2981975425175007 8 0.22742448960358497 1.0804468083261254 9 0.17499911051365868 0.9353077348308558 >10 1.0032337728399752 10.976285485740176 >50 0.07451264120687286 3.0445288882223895 >100 0.038013289224328124 4.087728731971761 >500 0.002249975601004808 0.9969057188737646 >1k 8.289383793175609E-4 0.8663853313011273 >5k 2.3683953694787454E-4 0.9250351175271938 >10k+ 1.1841976847393727E-4 0.753164520960639 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10710 0.7516533214865471 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7992 0.5608976046050872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 0.3622814608322648 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2634 0.1848603967129379 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2104 0.1476637337448828 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.13966294208760305 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1835 0.1287846727290209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.01823829585945E-5 0.0 0.0 0.0 0.0 3 7.01823829585945E-5 0.0 0.0 0.0 0.0 4 7.01823829585945E-5 0.0 0.0 0.0 0.0 5 7.01823829585945E-5 0.0 0.0 0.0 0.0 6 1.40364765917189E-4 0.0 0.0 0.0 0.0 7 2.105471488757835E-4 0.0 0.0 0.0 0.0 8 2.105471488757835E-4 0.0 0.0 0.0 0.0 9 2.105471488757835E-4 0.0 0.0 1.40364765917189E-4 0.0 10-11 2.105471488757835E-4 0.0 0.0 1.40364765917189E-4 0.0 12-13 2.105471488757835E-4 0.0 0.0 1.40364765917189E-4 0.0 14-15 2.80729531834378E-4 0.0 0.0 1.40364765917189E-4 0.0 16-17 3.1582072331367525E-4 0.0 0.0 1.40364765917189E-4 0.0 18-19 4.5618548923086427E-4 0.0 0.0 2.8072953183437804E-4 0.0 20-21 4.912766807101615E-4 0.0 0.0 5.263678721894588E-4 0.0 22-23 4.912766807101615E-4 0.0 0.0 9.123709784617285E-4 0.0 24-25 4.912766807101615E-4 0.0 0.0 0.0014387388506511872 0.0 26-27 4.912766807101615E-4 0.0 0.0 0.0023511098291129156 0.0 28-29 5.965502551480532E-4 0.0 0.0 0.0045267637008293456 0.0 30-31 6.316414466273505E-4 0.0 0.0 0.007614788551007503 0.0 32-33 6.316414466273505E-4 0.0 0.0 0.011088816507457931 0.0 34-35 7.018238295859449E-4 0.0 0.0 0.015019029953139224 0.0 36-37 7.720062125445395E-4 0.0 0.0 0.020142343909116622 0.0 38-39 7.720062125445395E-4 0.0 0.0 0.02845895628971007 0.0 40-41 7.720062125445395E-4 0.0 0.0 0.04161815309444654 0.0 42-43 8.070974040238367E-4 0.0 0.0 0.0548826234736209 0.0 44-45 9.123709784617285E-4 0.0 0.0 0.0676558171720851 0.0 46-47 0.0010527357443789174 0.0 0.0 0.09250038073942754 0.0 48-49 0.0010527357443789174 0.0 0.0 0.10783523141588044 0.0 50-51 0.0010527357443789174 0.0 0.0 0.12583701264475994 0.0 52-53 0.0010527357443789174 7.01823829585945E-5 0.0 0.1467864539579004 0.0 54-55 0.0010527357443789174 7.01823829585945E-5 0.0 0.17464885999246244 0.0 56-57 0.001122918127337512 7.01823829585945E-5 0.0 0.24062029997354123 0.0 58-59 0.001122918127337512 7.01823829585945E-5 0.0 0.2989769514036126 0.0 60-61 0.001122918127337512 7.01823829585945E-5 0.0 0.34519205058184704 0.0 62-63 0.001122918127337512 7.01823829585945E-5 0.0 0.39716210516268624 0.0 64-65 0.001122918127337512 7.01823829585945E-5 0.0 0.44569322297855435 0.0 66-67 0.0014738300421304844 7.01823829585945E-5 0.0 0.5275960638912341 0.0 68-69 0.0014738300421304844 7.01823829585945E-5 0.0 0.6299570694363443 0.0 70-71 0.0014738300421304844 7.01823829585945E-5 0.0 0.6816814856768283 0.0 72-73 0.001544012425089079 7.01823829585945E-5 0.0 0.7412312376171959 0.0 74-75 0.0016141948080476735 7.01823829585945E-5 0.0 0.852224676266213 0.0 76-77 0.0016141948080476735 7.01823829585945E-5 0.0 0.9829393645265952 0.0 78-79 0.0016141948080476735 1.0527357443789175E-4 0.0 1.110636210319758 0.0 80-81 0.0017545595739648626 1.40364765917189E-4 0.0 1.2107513796101932 0.0 82-83 0.0017545595739648626 1.40364765917189E-4 0.0 1.3747676085844285 0.0 84-85 0.001824741956923457 1.40364765917189E-4 0.0 1.6468997985063785 0.0 86-87 0.0018949243398820514 1.40364765917189E-4 0.0 1.8906783057130565 0.0 88-89 0.0018949243398820514 1.40364765917189E-4 0.0 2.1802508178002173 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13320 0.0 28.868362 1 GTGTAGC 750 0.0 25.983665 1 GGTATCA 9255 0.0 25.421818 1 GTACATG 9515 0.0 25.2267 1 TATCAAC 15765 0.0 24.241259 2 ATCAACG 15795 0.0 24.22531 3 TACATGG 9670 0.0 24.1842 2 GAGTACT 7415 0.0 24.123953 12-13 TCAACGC 16040 0.0 23.855288 4 ACATGGG 9630 0.0 23.840422 3 CAACGCA 16000 0.0 23.824125 5 AACGCAG 16280 0.0 23.41355 6 GTACTTT 8015 0.0 22.259155 14-15 AGTACTT 7700 0.0 21.935299 12-13 CATGGGG 3530 0.0 21.813904 4 ACGCAGA 17790 0.0 21.34608 7 CGCAGAG 17885 0.0 21.152227 8 GTACCTG 1215 0.0 21.124931 1 ATGGGGA 1875 0.0 20.532711 5 CATGGGA 5945 0.0 20.468273 4 >>END_MODULE