FastQCFastQC Report
Thu 26 May 2016
SRR937015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424859
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103450.72603675170666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86290.6056037825497119No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56400.39582863988647293No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29880.20970496028028035No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27410.19236991168950754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22750.15966492123080248No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18930.1328552509406194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.11439728422250903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA550.002579701834.545812
GGTATCA105750.026.1907061
GTATCAA144550.025.5365371
GTACATG91700.024.8156281
TACCTGG10350.023.864982
TACATGG95000.023.5002442
GAGTACT87000.023.39371512-13
ACATGGG95450.022.6919483
TGCACCG2755.2750693E-1122.453995
GTACTTT91400.022.32068314-15
GTACCTG12250.021.717561
TATCAAC172450.021.5122572
TTAGGGC6450.021.3560623
AGTACTT89800.021.34198612-13
ATCAACG173650.021.3354933
CATGGGG37050.021.2815254
TCAACGC175200.021.0925164
CAACGCA176450.020.970015
AACGCAG180150.020.5385996
ACTTTTT103300.019.70373716-17
Produced by FastQC (version 0.11.5)