##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937015_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1424859 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1231195507766 33.0 31.0 34.0 31.0 34.0 2 32.09620039596901 33.0 31.0 34.0 30.0 34.0 3 32.27303122624765 33.0 31.0 34.0 30.0 34.0 4 36.01614405355197 37.0 35.0 37.0 35.0 37.0 5 35.95210122545459 37.0 35.0 37.0 35.0 37.0 6 36.0144652909516 37.0 35.0 37.0 35.0 37.0 7 35.953325206213385 37.0 35.0 37.0 35.0 37.0 8 35.937049209781456 37.0 35.0 37.0 35.0 37.0 9 37.603389528367366 39.0 37.0 39.0 35.0 39.0 10-11 37.531396089016525 39.0 37.0 39.0 35.0 39.0 12-13 37.43576276670183 39.0 37.0 39.0 34.5 39.0 14-15 38.59621092332645 40.0 38.0 41.0 34.0 41.0 16-17 38.52290507341428 40.0 38.0 41.0 34.0 41.0 18-19 38.62023751122041 40.0 38.0 41.0 34.0 41.0 20-21 38.711097729670094 40.0 38.0 41.0 34.0 41.0 22-23 38.64188631997973 40.0 38.0 41.0 34.0 41.0 24-25 38.53318503795814 40.0 38.0 41.0 34.0 41.0 26-27 38.35857267280482 40.0 38.0 41.0 34.0 41.0 28-29 38.17062600580128 40.0 38.0 41.0 34.0 41.0 30-31 37.9957350165876 40.0 37.0 41.0 33.0 41.0 32-33 37.9413271769347 40.0 37.0 41.0 33.0 41.0 34-35 37.83364283764218 40.0 37.0 41.0 33.0 41.0 36-37 37.64352648226948 40.0 37.0 41.0 33.0 41.0 38-39 37.4648803846556 40.0 36.0 41.0 33.0 41.0 40-41 37.25628956970479 40.0 36.0 41.0 32.0 41.0 42-43 36.999853669731536 40.0 36.0 41.0 31.5 41.0 44-45 36.5978275745179 39.0 35.5 41.0 30.5 41.0 46-47 36.22792079777719 39.0 35.0 41.0 30.0 41.0 48-49 35.81337732365097 39.0 35.0 41.0 28.5 41.0 50-51 35.508845085724275 39.0 35.0 40.0 27.5 41.0 52-53 35.23079266088784 39.0 35.0 40.0 27.0 41.0 54-55 34.98084828042634 38.0 34.5 40.0 26.0 41.0 56-57 34.71020044790397 38.0 34.0 40.0 26.0 41.0 58-59 34.38676669059886 38.0 34.0 40.0 25.0 41.0 60-61 34.05186969377321 37.5 34.0 40.0 24.5 41.0 62-63 33.67312169133928 37.0 33.0 40.0 23.0 41.0 64-65 33.254466231395526 36.0 33.0 39.0 22.0 40.5 66-67 32.87107145338591 36.0 32.0 39.0 21.5 40.0 68-69 32.434777756957004 35.0 32.0 38.5 20.5 40.0 70-71 31.962894223217873 35.0 31.0 38.0 19.0 40.0 72-73 31.627089417268657 35.0 31.0 37.0 18.0 39.0 74-75 31.191791959765844 35.0 31.0 37.0 16.5 39.0 76-77 29.80091924885199 33.5 29.5 35.0 13.0 37.5 78-79 30.517260655264835 34.0 30.5 36.0 12.5 38.0 80-81 30.361833697228988 34.0 30.5 35.5 9.0 37.0 82-83 30.033289960620664 34.0 30.0 35.0 7.0 37.0 84-85 29.67603250567249 34.0 30.0 35.0 3.5 36.0 86-87 29.304842794971293 34.0 29.5 35.0 2.0 36.0 88-89 28.948006083408956 34.0 29.0 35.0 2.0 35.5 90-91 28.528027334634515 34.0 29.0 35.0 2.0 35.0 92-93 28.094146859443633 34.0 29.0 35.0 2.0 35.0 94-95 27.644347265238174 33.0 27.0 35.0 2.0 35.0 96-97 27.016980978468748 33.0 26.5 35.0 2.0 35.0 98-99 26.298372330174423 33.0 25.0 35.0 2.0 35.0 100-101 23.35872742495924 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24624816123422733 1101 2 0.21363758223660767 1101 3 0.19138886878389627 1101 4 0.1749806365010116 1101 5 0.1460558261645062 1101 6 0.18948168293785983 1101 7 0.22605199135210796 1101 8 0.2527298508347897 1101 9 0.2774236549555269 1101 10-11 0.3111059140622672 1101 12-13 0.3671937874847515 1101 14-15 0.44643591856952725 1101 16-17 0.4550699655241033 1101 18-19 0.49202400228094945 1101 20-21 0.44248762840976497 1101 22-23 0.3054567309641172 1101 24-25 0.28329667338514497 1101 26-27 0.3782534705844185 1101 28-29 0.28615632684913095 1101 30-31 0.46641997385680156 1101 32-33 0.5778774019744475 1101 34-35 0.5217407908323324 1101 36-37 0.45701740591466233 1101 38-39 0.42358003807649425 1101 40-41 0.33069662712340175 1101 42-43 0.5471323101015244 1101 44-45 0.5293654378269181 1101 46-47 0.5267745506237773 1101 48-49 0.37922526593089145 1101 50-51 0.4476696549473047 1101 52-53 0.483291512326808 1101 54-55 0.46196722282931546 1101 56-57 0.4254561131973773 1101 58-59 0.3563925166119759 1101 60-61 0.3210738478001858 1101 62-63 0.4903145093736896 1101 64-65 0.39142751192971303 1101 66-67 0.47036149933465055 1101 68-69 0.5048722064675886 1101 70-71 0.39028417636677304 1101 72-73 0.5736943699218635 1101 74-75 0.43505719556133826 1101 76-77 0.5307890061153913 1101 78-79 0.639414485045716 1101 80-81 0.846978139895679 1101 82-83 0.8094666721178498 1101 84-85 0.9074835868158786 1101 86-87 0.774256353329001 1101 88-89 0.7413543121834962 1101 90-91 0.6530882971788223 1101 92-93 0.5672480854765034 1101 94-95 0.6709541710148699 1101 96-97 0.8286859875669954 1101 98-99 0.7364932667129978 1101 100-101 0.5988565611972874 1102 1 0.17938469355805609 1102 2 0.057172235004848915 1102 3 0.1398593373534922 1102 4 0.14035213514856082 1102 5 0.12526491715438226 1102 6 0.12735777691290906 1102 7 0.1675435092217583 1102 8 0.13233944794299646 1102 9 0.21312222334609743 1102 10-11 0.2358891697757457 1102 12-13 0.23582672113225556 1102 14-15 0.3646061066686386 1102 16-17 0.29674273309042576 1102 18-19 0.2450281399555223 1102 20-21 0.21720652561320009 1102 22-23 0.23330752585835768 1102 24-25 0.22887784474421125 1102 26-27 0.20776682044657235 1102 28-29 0.10920955882208716 1102 30-31 0.1730800030180717 1102 32-33 0.22714471297814498 1102 34-35 0.07597385818694846 1102 36-37 0.16833496782427204 1102 38-39 0.2294653042500272 1102 40-41 0.23237046106545733 1102 42-43 0.34045314086290546 1102 44-45 0.6130825980683383 1102 46-47 0.5604503204479414 1102 48-49 0.4493213559548721 1102 50-51 0.5367803864468854 1102 52-53 0.48408480017511124 1102 54-55 0.5470382017553987 1102 56-57 0.4738852938858642 1102 58-59 0.4726464411822988 1102 60-61 0.4813381585967704 1102 62-63 0.5456264276947778 1102 64-65 0.4534835732162392 1102 66-67 0.5616244641736543 1102 68-69 0.3664701363315146 1102 70-71 0.34411096140114594 1102 72-73 0.43302488419523044 1102 74-75 0.23605200108105961 1102 76-77 0.2364749420549188 1102 78-79 0.38901596129253946 1102 80-81 0.4782291695293033 1102 82-83 0.5063186078974198 1102 84-85 0.5410232228399607 1102 86-87 0.4393502866764152 1102 88-89 0.4485625678457126 1102 90-91 0.4384511281056298 1102 92-93 0.5548724874345332 1102 94-95 0.6704548200685636 1102 96-97 0.6955935799571762 1102 98-99 0.6407710056409783 1102 100-101 0.701074144229338 1103 1 -0.0021343351235927344 1103 2 -0.1889823962091981 1103 3 -0.0796292848998732 1103 4 0.10527417997916189 1103 5 0.07449523105023559 1103 6 0.07654182459432235 1103 7 0.19545904616499143 1103 8 0.1527054273069055 1103 9 0.0651203454821001 1103 10-11 0.14010449818081838 1103 12-13 0.19140826363423713 1103 14-15 0.22206687393937585 1103 16-17 0.24232669096095094 1103 18-19 0.3293578411642315 1103 20-21 0.2662231984600325 1103 22-23 0.38235719740225704 1103 24-25 0.33367643362466737 1103 26-27 0.43367096773643254 1103 28-29 0.25247918539250236 1103 30-31 0.2245809334452531 1103 32-33 0.2529922181745974 1103 34-35 0.09992853090451348 1103 36-37 0.21741610719784177 1103 38-39 0.20719509595000574 1103 40-41 0.20381212991502196 1103 42-43 0.13472208364589022 1103 44-45 0.2639701609614704 1103 46-47 0.0544515315341485 1103 48-49 0.10164572294120688 1103 50-51 -0.03438895810374021 1103 52-53 0.02359784826816025 1103 54-55 0.12456557876721774 1103 56-57 0.09126710241857694 1103 58-59 0.20625289259766788 1103 60-61 0.24281847334309958 1103 62-63 0.09564037559765382 1103 64-65 0.06472303149330116 1103 66-67 0.0654383651299284 1103 68-69 0.23975190778321576 1103 70-71 0.3071832081361485 1103 72-73 0.18107796106859197 1103 74-75 0.13053747655146353 1103 76-77 0.2562671863405299 1103 78-79 0.22171647940610129 1103 80-81 0.36636290610444533 1103 82-83 0.5159001367916929 1103 84-85 0.47733743847454235 1103 86-87 0.2849825519179028 1103 88-89 0.5760264989090516 1103 90-91 0.839043743881728 1103 92-93 0.8113385310948722 1103 94-95 0.6932909390135578 1103 96-97 0.7388931434898716 1103 98-99 0.5288261576901263 1103 100-101 0.7855793848987034 1104 1 0.25249212375488383 1104 2 0.13347875414956434 1104 3 0.13938956688046744 1104 4 0.10816123341804484 1104 5 0.12103439971308916 1104 6 0.14814502808770413 1104 7 0.23560524289253237 1104 8 0.18902631510903234 1104 9 0.2222862533198935 1104 10-11 0.20236903533208306 1104 12-13 0.18119551494029196 1104 14-15 0.2779434181054228 1104 16-17 0.3736099876897896 1104 18-19 0.4137927211069936 1104 20-21 0.2808004579352499 1104 22-23 0.2165555772150114 1104 24-25 0.20545334715506414 1104 26-27 0.3098500107822133 1104 28-29 0.3264230486520887 1104 30-31 0.275164300432607 1104 32-33 0.3117831078023343 1104 34-35 0.194923210804113 1104 36-37 0.13028152986459673 1104 38-39 0.1483287844484451 1104 40-41 0.3228274779897333 1104 42-43 0.3010638277167885 1104 44-45 0.2385103918003466 1104 46-47 0.3863793256932766 1104 48-49 0.35702123720221124 1104 50-51 0.32544050363487287 1104 52-53 0.3473177509757903 1104 54-55 0.32970293324818556 1104 56-57 0.4481398054168295 1104 58-59 0.24559803157492155 1104 60-61 0.27460040270471353 1104 62-63 0.3783706105487852 1104 64-65 0.37336704209293003 1104 66-67 0.46837225758092416 1104 68-69 0.4011281633545707 1104 70-71 0.3046017147092819 1104 72-73 0.5357025758914737 1104 74-75 0.542399548569211 1104 76-77 0.6185040626578768 1104 78-79 0.5149965564138661 1104 80-81 0.6367588064000493 1104 82-83 0.7773503389923846 1104 84-85 0.7838499052123851 1104 86-87 0.6597857450845694 1104 88-89 0.6659026336947633 1104 90-91 0.7681964187765722 1104 92-93 0.7685839177287868 1104 94-95 0.7967644021315223 1104 96-97 0.922719485086418 1104 98-99 0.9883738477338895 1104 100-101 0.8407181224213609 1105 1 0.20265883226328896 1105 2 0.0927117378389326 1105 3 0.15810790150303689 1105 4 0.10120233729216466 1105 5 0.11905445740795528 1105 6 0.05657577619664522 1105 7 0.19312590145167263 1105 8 0.09693159901899406 1105 9 0.22066058517962972 1105 10-11 0.19217536970317894 1105 12-13 0.2787899763692181 1105 14-15 0.403441855093682 1105 16-17 0.3318604340369973 1105 18-19 0.2902105080488866 1105 20-21 0.2871805788217543 1105 22-23 0.40218461988040843 1105 24-25 0.32548262362441704 1105 26-27 0.32343986579742534 1105 28-29 0.20916445952133955 1105 30-31 0.3030856923477643 1105 32-33 0.24634815980289915 1105 34-35 0.32546389519893637 1105 36-37 0.349540706053979 1105 38-39 0.31678338871698486 1105 40-41 0.23602797125492003 1105 42-43 0.24439652811425816 1105 44-45 0.20291582077580728 1105 46-47 0.18857837425050405 1105 48-49 0.4011916643149718 1105 50-51 0.45214024438604383 1105 52-53 0.5589018365045391 1105 54-55 0.47055962255186046 1105 56-57 0.5459434755102706 1105 58-59 0.5922640100831345 1105 60-61 0.6306219378162936 1105 62-63 0.6116894585715329 1105 64-65 0.5951719797572466 1105 66-67 0.61018790842801 1105 68-69 0.6555208019282261 1105 70-71 0.4230208278429579 1105 72-73 0.5310900027268524 1105 74-75 0.49254438043553606 1105 76-77 0.47399524849506847 1105 78-79 0.5882688645355358 1105 80-81 0.6208714791777332 1105 82-83 0.6179556380673219 1105 84-85 0.6241340636774382 1105 86-87 0.7020431341859243 1105 88-89 0.895745644362492 1105 90-91 0.9940752969423663 1105 92-93 0.8645873313345298 1105 94-95 0.7219072056295772 1105 96-97 0.9479649176471021 1105 98-99 0.8712734925676671 1105 100-101 1.122860750481486 1106 1 0.3138452044486044 1106 2 0.2651909266106287 1106 3 0.2726009335431385 1106 4 0.23653041479688142 1106 5 0.14021038026547217 1106 6 0.18220843596662917 1106 7 0.21961843935105918 1106 8 0.32811564706789653 1106 9 0.4093872672602785 1106 10-11 0.28980715844545557 1106 12-13 0.3808533279309714 1106 14-15 0.4963379541563384 1106 16-17 0.48764981074444336 1106 18-19 0.5197252608188734 1106 20-21 0.5773709957372759 1106 22-23 0.5491294535711759 1106 24-25 0.5488708690427515 1106 26-27 0.43228823002938555 1106 28-29 0.45496674984261887 1106 30-31 0.5119847269412787 1106 32-33 0.4519528357141098 1106 34-35 0.36283616584357503 1106 36-37 0.28286311664408714 1106 38-39 0.46552026768443255 1106 40-41 0.4765784461063447 1106 42-43 0.4095855709626477 1106 44-45 0.3396567010267759 1106 46-47 0.21056318675184826 1106 48-49 -0.03340718921575103 1106 50-51 -0.11607342122201914 1106 52-53 0.011627907190735698 1106 54-55 0.03961526415746164 1106 56-57 0.1492364208663659 1106 58-59 -0.0711670120081962 1106 60-61 -0.016788770066455072 1106 62-63 0.10676454021729853 1106 64-65 0.059240254928717206 1106 66-67 0.007374970525908964 1106 68-69 0.15421713937189452 1106 70-71 -0.011148008104942875 1106 72-73 0.3000143096866488 1106 74-75 0.3429448490316851 1106 76-77 0.14587926990015276 1106 78-79 0.14577475208606927 1106 80-81 0.16422028756053564 1106 82-83 0.32679342852548743 1106 84-85 0.13751800106813405 1106 86-87 0.1394490105868833 1106 88-89 0.29784035082772675 1106 90-91 0.48557440157404486 1106 92-93 0.579250381281998 1106 94-95 0.42831930956453945 1106 96-97 0.6141268000300961 1106 98-99 0.46868490711351285 1106 100-101 0.6712095928888111 1107 1 0.10794226079894997 1107 2 0.010223430514734844 1107 3 0.03873597210922952 1107 4 0.022519234683066713 1107 5 0.10471561413434216 1107 6 0.15506048244148474 1107 7 0.14304778980376653 1107 8 0.05497613589756156 1107 9 0.1339877080953471 1107 10-11 0.1465393916466553 1107 12-13 0.1958065206328925 1107 14-15 0.20480405483323239 1107 16-17 0.27072152047493603 1107 18-19 0.26580621467645926 1107 20-21 0.06753880918973465 1107 22-23 0.17392433116820882 1107 24-25 0.18677318691891998 1107 26-27 0.27208909996627995 1107 28-29 0.23776608048847692 1107 30-31 0.21903948491500103 1107 32-33 0.1778383471023517 1107 34-35 0.30483255088423533 1107 36-37 0.3234925777246076 1107 38-39 0.37405090125215423 1107 40-41 0.42006551298868544 1107 42-43 0.3364211964707451 1107 44-45 0.3917045726985151 1107 46-47 0.3216312759357436 1107 48-49 0.2924207733607602 1107 50-51 0.33798530650225445 1107 52-53 0.603442566493392 1107 54-55 0.4276367304229609 1107 56-57 0.3306403325093825 1107 58-59 0.3044848120935839 1107 60-61 0.361655009493667 1107 62-63 0.36542521733773015 1107 64-65 0.3449251276927683 1107 66-67 0.28597714899985505 1107 68-69 0.25904509356750793 1107 70-71 0.4162399183778511 1107 72-73 0.37595136357859005 1107 74-75 0.5399327554003257 1107 76-77 0.4776649313064141 1107 78-79 0.37685613895655834 1107 80-81 0.39819671489678043 1107 82-83 0.4160600164117412 1107 84-85 0.4417668354253337 1107 86-87 0.36212603603966187 1107 88-89 0.41049466405529245 1107 90-91 0.5415255353694484 1107 92-93 0.7142090495019247 1107 94-95 0.6551873411996212 1107 96-97 0.9539866155945376 1107 98-99 0.8256076659923721 1107 100-101 0.9200350104668971 1108 1 0.026932760372496034 1108 2 -0.07394877026127489 1108 3 0.0408061480667854 1108 4 0.08138391646561871 1108 5 0.0077979528070457604 1108 6 0.06740807873991628 1108 7 0.07776502023471465 1108 8 0.10194728950668264 1108 9 0.22140099883205977 1108 10-11 0.22102332670652913 1108 12-13 0.16511846691381749 1108 14-15 0.13196778065694303 1108 16-17 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-1.095780967831157 2316 74-75 -1.1010229468456956 2316 76-77 -1.4771620154560168 2316 78-79 -1.1393123336635291 2316 80-81 -1.3608295925166232 2316 82-83 -1.2653490424166733 2316 84-85 -1.1134946472555036 2316 86-87 -1.316115338975905 2316 88-89 -1.5241547318844226 2316 90-91 -1.5484303191025361 2316 92-93 -1.6940222232454758 2316 94-95 -1.60125112491626 2316 96-97 -1.436259852071288 2316 98-99 -1.3864707718925153 2316 100-101 -1.457380192754755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 9.0 5 43.0 6 96.0 7 204.0 8 479.0 9 942.0 10 1584.0 11 2546.0 12 3900.0 13 5335.0 14 6878.0 15 9619.0 16 12856.0 17 18457.0 18 16880.0 19 9574.0 20 7749.0 21 7626.0 22 7964.0 23 8616.0 24 9979.0 25 11768.0 26 14151.0 27 17851.0 28 21703.0 29 27214.0 30 34189.0 31 42838.0 32 53894.0 33 70953.0 34 97151.0 35 139007.0 36 210615.0 37 310195.0 38 224705.0 39 17281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5684699817565 19.237100217110985 14.917061919098257 21.27736788203426 2 15.785894307316878 21.221353579973457 39.70953707427348 23.283215038436193 3 19.992293812099557 25.91012675907074 29.979176448542244 24.11840298028746 4 13.015719505296683 17.319388512787977 36.719481434589085 32.945410547326254 5 13.367849392635264 38.42357479134558 33.02066752570808 15.18790829031108 6 30.910162788494265 36.45283204255871 17.37131146678083 15.265693702166185 7 28.084463094243013 34.205489806359786 19.995732911116118 17.714314188281087 8 27.161634940720454 33.88012427896374 19.257694971923538 19.70054580839227 9 27.42411705298559 16.752745359365385 19.986328471799666 35.836809115849356 10-11 24.976374847046216 27.192454548995563 26.458627295271775 21.372543308686442 12-13 26.890418953019246 24.707767381748035 27.293935254214567 21.107878411018145 14-15 23.524327670316854 25.480556321713237 26.819355459031385 24.175760548938527 16-17 21.553781812796917 28.970831499818576 27.175425778971814 22.299960908412693 18-19 21.347656154047524 26.828830080730796 30.80789046495127 21.015623300270413 20-21 22.6794279864952 25.736080849141985 30.677700056813933 20.906791107548884 22-23 22.242025351280372 25.942496766346707 30.544215252175828 21.271262630197093 24-25 22.00616222513879 25.979955222099786 30.948512433236715 21.065370119524708 26-27 21.994597265365652 26.082406792650932 30.727719860699565 21.195276081283854 28-29 21.453577163647797 26.2146129745876 31.17399018539938 21.15781967636523 30-31 21.58535267105701 26.163555475691435 30.94261575890645 21.308476094345107 32-33 21.525654261392944 26.251762464987564 30.710263739857712 21.512319533761776 34-35 21.968038870752153 26.068543880316486 30.66932102866002 21.29409622027134 36-37 22.231381351031157 25.79084339784729 30.64798906238595 21.329786188735607 38-39 22.036644397327677 25.88191284708551 30.797836091208136 21.283606664378674 40-41 21.960792845955954 26.1073268031357 30.63868224434515 21.293198106563196 42-43 21.778233346937242 26.33370659279615 30.2836362513427 21.604423808923904 44-45 22.011756288098187 26.542850515614052 29.78539212249391 21.66000107379385 46-47 22.34529500153349 26.65355190029596 29.14860929903843 21.852543799132125 48-49 22.504012683378054 26.879609673111055 28.781660194672163 21.834717448838724 50-51 22.481967494995114 27.260831346292523 28.566681229526463 21.6905199291859 52-53 22.532918458233922 27.38084418216278 28.386706918955834 21.699530440647465 54-55 22.059375198489914 27.236272919992377 28.472990106473063 22.231361775044643 56-57 22.331929330328116 27.68486185782478 28.093289525351533 21.88991928649557 58-59 22.542552799510123 27.132658056388493 28.204948248847682 22.119840895253702 60-61 22.317106037020245 26.99860756490895 28.781577185400202 21.902709212670597 62-63 22.505665472864333 27.003233302382903 28.728912825760304 21.762188398992464 64-65 22.2919212243755 27.157953394621355 28.613888972717966 21.936236408285183 66-67 22.124189130292894 27.451172361616134 28.397792342961655 22.02684616512932 68-69 22.17872084185172 27.533426114443603 28.319679350728737 21.968173692975938 70-71 22.16999091488734 27.494624904858973 28.154234262560106 22.18114991769358 72-73 22.232067254397542 27.412967673632156 27.960039540768435 22.39492553120187 74-75 22.147672673831845 27.83232527683538 27.738105097083633 22.28189695224914 76-77 22.35789689403374 27.574218981657427 27.881583698546063 22.18630042576277 78-79 22.351950776762976 27.599114436516547 27.713125997688888 22.33580878903159 80-81 22.284616791017147 27.91749879895719 27.679641438275524 22.118242971750142 82-83 22.199389265188184 27.76864225552184 27.754254685259617 22.27771379403035 84-85 22.28924012853495 27.64754164805329 27.57290098127737 22.490317242134395 86-87 22.429376885526004 27.474594417807825 27.695953459857968 22.400075236808203 88-89 22.43778608435181 27.394546202315766 27.510488882276068 22.657178831056356 90-91 22.146339443530707 27.51746927852836 27.854099196516707 22.482092081424227 92-93 22.063103108133195 27.68253830117166 27.686924701645815 22.567433889049333 94-95 21.93500051759562 27.649887795797124 27.505943576813824 22.909168109793434 96-97 22.082627992959228 27.5340668873224 27.31656939285985 23.06673572685852 98-99 21.923117683760907 27.743403234217208 27.10557141323122 23.227907668790667 100-101 21.754550833105824 27.863268318256885 27.332124316331065 23.05005653230623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1165.0 1 827.5 2 561.0 3 645.0 4 577.0 5 549.0 6 933.0 7 1970.0 8 3190.5 9 3720.5 10 3306.5 11 2738.0 12 2645.5 13 2749.0 14 2909.5 15 3036.0 16 3135.0 17 3274.5 18 3412.0 19 3582.0 20 3773.5 21 4164.0 22 4732.5 23 5445.5 24 6362.0 25 7526.5 26 9180.5 27 11143.5 28 12828.5 29 14504.5 30 16632.0 31 19100.5 32 21414.5 33 23432.5 34 26344.5 35 28695.5 36 31263.0 37 34532.0 38 35758.5 39 36396.0 40 37819.0 41 40138.0 42 42830.0 43 45018.5 44 48116.0 45 50674.0 46 50615.0 47 52404.5 48 62682.0 49 63373.5 50 54008.5 51 50374.5 52 48225.5 53 47780.0 54 46542.5 55 42756.0 56 38447.5 57 34840.5 58 32364.0 59 29500.0 60 25126.0 61 20525.5 62 16441.5 63 12660.0 64 9186.0 65 6719.0 66 4919.0 67 3679.0 68 2691.0 69 1892.5 70 1394.0 71 1012.5 72 732.5 73 549.5 74 425.5 75 323.5 76 220.5 77 114.5 78 57.5 79 33.0 80 22.5 81 14.5 82 12.0 83 7.0 84 4.5 85 4.0 86 2.0 87 1.0 88 1.5 89 1.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01698413667597987 2 0.002105471488757835 3 0.002105471488757835 4 3.509119147929725E-4 5 0.0010527357443789174 6 2.8072953183437804E-4 7 0.0 8 0.0 9 0.0 10-11 3.5091191479297255E-5 12-13 6.667326381066478E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0022809274461543213 22-23 0.0 24-25 0.003438936764971131 26-27 0.0017545595739648623 28-29 0.0030178424672195636 30-31 0.015229577102015005 32-33 5.614590636687561E-4 34-35 0.0024212922120715103 36-37 0.00357930153088832 38-39 0.002105471488757835 40-41 0.002982751275740266 42-43 0.0016492859995269706 44-45 3.1582072331367525E-4 46-47 1.4036476591718902E-4 48-49 1.4036476591718902E-4 50-51 1.0527357443789174E-4 52-53 8.772797869824312E-4 54-55 0.0027020217439058885 56-57 3.1582072331367525E-4 58-59 8.070974040238368E-4 60-61 0.0011931005102961065 62-63 0.0 64-65 1.4036476591718902E-4 66-67 0.0 68-69 0.0 70-71 3.5091191479297255E-5 72-73 3.5091191479297255E-5 74-75 3.509119147929725E-4 76-77 3.1582072331367525E-4 78-79 2.4563834035508077E-4 80-81 0.003895122254201995 82-83 0.001263282893254701 84-85 0.0022809274461543213 86-87 0.0017194683824855654 88-89 0.0011931005102961065 90-91 3.5091191479297255E-5 92-93 3.5091191479297255E-5 94-95 1.0527357443789174E-4 96-97 0.0010527357443789174 98-99 0.0022458362546750243 100-101 7.01823829585945E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1424859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.25152147231821 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21190437568053 45.35002044227491 2 12.21312451361211 13.984399206703193 3 3.64256808489948 6.256276947810109 4 1.56644770541869 3.5872605776816697 5 0.8287341662208418 2.372314595611813 6 0.5239050658062406 1.7996617274677364 7 0.3582188359765282 1.4356001365789328 8 0.25086533228053914 1.1489937566175623 9 0.1780574777695626 0.9174655360647843 >10 1.0966833041772257 11.534968747092316 >50 0.08199947197021763 3.204113164646742 >100 0.043196609257544254 4.441949334995239 >500 0.0028224659828153184 1.082656161194261 >1k 0.0011044432106668638 1.1550205898788868 >5k 2.454318245926364E-4 1.0024932358261458 >10k+ 1.227159122963182E-4 0.7268058395557838 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10345 0.72603675170666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8629 0.6056037825497119 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5640 0.39582863988647293 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2988 0.20970496028028035 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2741 0.19236991168950754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2275 0.15966492123080248 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1893 0.1328552509406194 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1630 0.11439728422250903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.40364765917189E-4 0.0 10-11 0.0 0.0 0.0 1.40364765917189E-4 0.0 12-13 0.0 0.0 0.0 1.40364765917189E-4 0.0 14-15 0.0 0.0 0.0 1.40364765917189E-4 0.0 16-17 0.0 0.0 0.0 2.105471488757835E-4 0.0 18-19 1.40364765917189E-4 0.0 0.0 4.5618548923086427E-4 0.0 20-21 2.105471488757835E-4 0.0 0.0 7.369150210652423E-4 0.0 22-23 2.105471488757835E-4 0.0 0.0 0.001122918127337512 0.0 24-25 2.80729531834378E-4 0.0 0.0 0.0017194683824855654 0.0 26-27 2.80729531834378E-4 0.0 0.0 0.002526565786509402 0.0 28-29 2.80729531834378E-4 0.0 0.0 0.004737310849705129 0.0 30-31 2.80729531834378E-4 0.0 0.0 0.008141156423196962 0.0 32-33 2.80729531834378E-4 0.0 0.0 0.011650275571126687 0.0 34-35 2.80729531834378E-4 0.0 0.0 0.016071765697518142 0.0 36-37 2.80729531834378E-4 0.0 0.0 0.021405626802371323 0.0 38-39 2.80729531834378E-4 0.0 0.0 0.03024860705515423 0.0 40-41 2.80729531834378E-4 0.0 0.0 0.04400435411503875 0.0 42-43 3.509119147929725E-4 0.0 0.0 0.05779519236640257 0.0 44-45 3.509119147929725E-4 0.0 0.0 0.07070875083078396 0.0 46-47 4.21094297751567E-4 0.0 0.0 0.09660605014250534 0.0 48-49 4.21094297751567E-4 0.0 0.0 0.11229181273375119 0.0 50-51 4.21094297751567E-4 0.0 0.0 0.13110069136665453 0.0 52-53 4.21094297751567E-4 0.0 0.0 0.1530677772326946 0.0 54-55 4.21094297751567E-4 0.0 0.0 0.18205310139459413 0.0 56-57 4.21094297751567E-4 0.0 0.0 0.2488667299711761 0.0 58-59 4.21094297751567E-4 0.0 0.0 0.30778484046491617 0.0 60-61 4.21094297751567E-4 0.0 0.0 0.354280669174985 0.0 62-63 4.21094297751567E-4 0.0 0.0 0.40677709162801373 0.0 64-65 4.21094297751567E-4 0.0 0.0 0.4560100332734678 0.0 66-67 4.21094297751567E-4 0.0 0.0 0.538193603717982 0.0 68-69 6.316414466273505E-4 0.0 0.0 0.6405897004545713 0.0 70-71 6.316414466273505E-4 0.0 0.0 0.6921737519291382 7.01823829585945E-5 72-73 6.316414466273505E-4 0.0 0.0 0.7524253276990915 7.01823829585945E-5 74-75 6.667326381066478E-4 0.0 0.0 0.8641205901776947 7.01823829585945E-5 76-77 7.01823829585945E-4 0.0 0.0 0.9955020110761836 7.01823829585945E-5 78-79 7.01823829585945E-4 0.0 0.0 1.1232339480608258 7.01823829585945E-5 80-81 7.01823829585945E-4 0.0 0.0 1.22338420854274 7.01823829585945E-5 82-83 7.01823829585945E-4 0.0 0.0 1.3872951639425375 7.01823829585945E-5 84-85 7.01823829585945E-4 0.0 0.0 1.6609713662895769 7.01823829585945E-5 86-87 7.01823829585945E-4 0.0 0.0 1.905592062091758 7.01823829585945E-5 88-89 7.01823829585945E-4 0.0 0.0 2.197164772093239 7.01823829585945E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 55 0.0025797018 34.54581 2 GGTATCA 10575 0.0 26.190706 1 GTATCAA 14455 0.0 25.536537 1 GTACATG 9170 0.0 24.815628 1 TACCTGG 1035 0.0 23.86498 2 TACATGG 9500 0.0 23.500244 2 GAGTACT 8700 0.0 23.393715 12-13 ACATGGG 9545 0.0 22.691948 3 TGCACCG 275 5.2750693E-11 22.45399 5 GTACTTT 9140 0.0 22.320683 14-15 GTACCTG 1225 0.0 21.71756 1 TATCAAC 17245 0.0 21.512257 2 TTAGGGC 645 0.0 21.356062 3 AGTACTT 8980 0.0 21.341986 12-13 ATCAACG 17365 0.0 21.335493 3 CATGGGG 3705 0.0 21.281525 4 TCAACGC 17520 0.0 21.092516 4 CAACGCA 17645 0.0 20.97001 5 AACGCAG 18015 0.0 20.538599 6 ACTTTTT 10330 0.0 19.703737 16-17 >>END_MODULE