FastQCFastQC Report
Thu 26 May 2016
SRR937014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215284
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96020.7901033832421065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70860.5830735860918106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.3762906448204699No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22270.1832493474776266No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18560.15272150378018637No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.14984151852571087No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16600.13659358635512356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.10318575740320782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109600.029.6545811
GGTATCA74550.027.7897761
TATTCCG1806.668415E-926.400345
GTACATG76050.025.3672281
TATCAAC131200.024.555122
ATCAACG132350.024.340763
TACATGG78600.024.241982
TCAACGC134050.024.0320744
CAACGCA135000.023.9011045
AACGCAG138100.023.4314776
ACATGGG80000.022.9852543
GAGTACT69700.022.90712512-13
CATGGGG28400.022.4189264
CCGTATA1100.002870226521.6002759
TACCTGG8450.021.3684542
AGTACTT71400.021.29687312-13
ACGCAGA151800.021.2550497
TACCGTG1353.7860667E-421.11947
GTACTTT75100.021.06888614-15
CGCAGAG152450.020.9470968
Produced by FastQC (version 0.11.5)