##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937014_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1215284 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800001481135272 33.0 31.0 34.0 30.0 34.0 2 31.8972059205914 33.0 31.0 34.0 30.0 34.0 3 31.899704924939357 33.0 31.0 34.0 30.0 34.0 4 35.488280928573076 37.0 35.0 37.0 33.0 37.0 5 35.502665220639784 37.0 35.0 37.0 33.0 37.0 6 35.578163622659396 37.0 35.0 37.0 33.0 37.0 7 35.52458190842634 37.0 35.0 37.0 33.0 37.0 8 35.472152188295084 37.0 35.0 37.0 33.0 37.0 9 37.10017576138581 39.0 37.0 39.0 33.0 39.0 10-11 36.928885346964165 39.0 37.0 39.0 33.0 39.0 12-13 36.8650307253284 39.0 37.0 39.0 33.0 39.0 14-15 37.98997189134391 40.0 38.0 41.0 33.0 41.0 16-17 37.939307190747186 40.0 38.0 41.0 33.0 41.0 18-19 37.951496934049985 40.0 38.0 41.0 33.0 41.0 20-21 37.90137819637221 40.0 38.0 41.0 33.0 41.0 22-23 37.79433943012498 40.0 37.5 41.0 32.5 41.0 24-25 37.62921629841255 40.0 37.0 41.0 32.0 41.0 26-27 37.684846093588 40.0 37.0 41.0 32.0 41.0 28-29 37.62304860427686 40.0 37.0 41.0 32.0 41.0 30-31 37.4395861378904 40.0 37.0 41.0 32.0 41.0 32-33 37.200024850158485 40.0 36.0 41.0 31.5 41.0 34-35 37.00509016822406 39.5 36.0 41.0 31.0 41.0 36-37 36.806544396206974 39.0 35.5 41.0 30.5 41.0 38-39 36.61624731338519 39.0 35.0 41.0 30.0 41.0 40-41 36.41786899193933 39.0 35.0 41.0 30.0 41.0 42-43 36.166994299274904 39.0 35.0 41.0 30.0 41.0 44-45 35.843272848157305 39.0 35.0 40.5 28.5 41.0 46-47 35.3834655109423 39.0 35.0 40.0 26.5 41.0 48-49 34.97004033625062 38.0 35.0 40.0 24.5 41.0 50-51 34.27901173717419 38.0 34.0 39.5 23.5 40.5 52-53 34.49989878908963 38.0 34.5 40.0 24.0 40.5 54-55 34.867412884560316 39.0 35.0 40.0 24.0 41.0 56-57 34.75420889273618 39.0 35.0 41.0 24.0 41.0 58-59 34.510059788493884 38.0 34.0 40.0 23.0 41.0 60-61 34.22912751258142 38.0 34.0 40.0 22.5 41.0 62-63 33.91102408984237 37.5 34.0 40.0 22.0 41.0 64-65 33.58429017414859 37.0 33.5 40.0 21.5 41.0 66-67 33.206757021404044 36.5 33.0 39.5 20.0 41.0 68-69 32.82419335727287 36.0 33.0 39.0 18.5 41.0 70-71 32.40183652545413 35.5 32.0 39.0 17.5 40.5 72-73 32.00761879527748 35.0 32.0 38.0 14.0 40.0 74-75 31.58466992077572 35.0 31.5 37.0 10.0 39.5 76-77 31.154513266034936 35.0 31.0 37.0 7.5 39.0 78-79 30.72244100967346 35.0 31.0 36.0 6.5 39.0 80-81 30.301084355590955 35.0 31.0 36.0 2.0 37.5 82-83 29.908047419368643 34.0 30.0 35.0 2.0 37.0 84-85 29.570552644484746 34.0 30.0 35.0 2.0 37.0 86-87 29.238258300117504 34.0 29.5 35.0 2.0 36.0 88-89 28.88715724061207 34.0 29.0 35.0 2.0 36.0 90-91 28.546420425184564 34.0 29.0 35.0 2.0 35.5 92-93 28.19082823438801 34.0 29.0 35.0 2.0 35.0 94-95 27.78954384324981 34.0 28.0 35.0 2.0 35.0 96-97 27.330438399583965 34.0 27.0 35.0 2.0 35.0 98-99 26.73587696373852 34.0 25.5 35.0 2.0 35.0 100-101 24.635546917428353 31.5 20.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16938369610593185 1101 2 0.2337555803004676 1101 3 0.22600370100828826 1101 4 0.18412126416033914 1101 5 0.16275090111007984 1101 6 0.12719474946568 1101 7 0.1287442334156239 1101 8 0.07634770323799955 1101 9 -0.02447015537639885 1101 10-11 0.12028840491129955 1101 12-13 0.043355641262152744 1101 14-15 0.4265464507014016 1101 16-17 0.48571195218240604 1101 18-19 0.2919349282301269 1101 20-21 0.28347251636175486 1101 22-23 0.3746638246157872 1101 24-25 0.26468448834957314 1101 26-27 0.23334641887687013 1101 28-29 0.30361403424913647 1101 30-31 0.4107302533228605 1101 32-33 0.5312555438152131 1101 34-35 0.42386920073057865 1101 36-37 0.3995124451624008 1101 38-39 0.472356534828819 1101 40-41 0.420269924860186 1101 42-43 0.5632889192323134 1101 44-45 0.6581864495890954 1101 46-47 0.8344486214453042 1101 48-49 0.9130202854647607 1101 50-51 0.7206641242559826 1101 52-53 0.8086697297096848 1101 54-55 0.8828439567275765 1101 56-57 0.9289598302828139 1101 58-59 0.7276378585143206 1101 60-61 0.8282721952259635 1101 62-63 0.8423388395903331 1101 64-65 0.9282255848012895 1101 66-67 0.7796452789603521 1101 68-69 0.9350105426189188 1101 70-71 0.9607725656174111 1101 72-73 1.059957087067069 1101 74-75 0.910484735768204 1101 76-77 0.9485432602233814 1101 78-79 0.8082327229657338 1101 80-81 0.6175067809590438 1101 82-83 0.7284375002313617 1101 84-85 0.7773539306162611 1101 86-87 0.676011693757232 1101 88-89 0.7226644890761875 1101 90-91 0.8000107344326857 1101 92-93 0.7929682918363916 1101 94-95 0.8582822167414115 1101 96-97 0.8913673483678402 1101 98-99 0.712266739402974 1101 100-101 0.913980625349943 1102 1 0.23627448978461985 1102 2 0.2809869848053381 1102 3 0.32748889709425555 1102 4 0.2672038388113833 1102 5 0.10504528814237801 1102 6 0.16517713961690106 1102 7 0.27624044458006125 1102 8 0.15957716390549592 1102 9 0.3092095727060169 1102 10-11 0.3747897062939458 1102 12-13 0.44735602014571185 1102 14-15 0.5008069413830611 1102 16-17 0.5034992310297213 1102 18-19 0.3703947116624775 1102 20-21 0.37774416763512875 1102 22-23 0.45547858899414706 1102 24-25 0.44507458740840633 1102 26-27 0.4099778832343546 1102 28-29 0.4369800165459381 1102 30-31 0.4283967287173951 1102 32-33 0.4582480457645417 1102 34-35 0.35988764247585436 1102 36-37 0.341787956655196 1102 38-39 0.4086406068935702 1102 40-41 0.35197204550988204 1102 42-43 0.4742663838973016 1102 44-45 0.6150561850295304 1102 46-47 0.7506913320551476 1102 48-49 0.8604419554077651 1102 50-51 0.6025543825557307 1102 52-53 0.5810959297821725 1102 54-55 0.8261620843838742 1102 56-57 0.7079118191469362 1102 58-59 0.7074887348884573 1102 60-61 0.5262704271027019 1102 62-63 0.6849405204056538 1102 64-65 0.6909432369312754 1102 66-67 0.5418749125964197 1102 68-69 0.461068396490667 1102 70-71 0.7546654019726589 1102 72-73 0.7306276560513325 1102 74-75 0.539870038380851 1102 76-77 0.7122527361560422 1102 78-79 0.6199162700845733 1102 80-81 0.6576392666548152 1102 82-83 0.4474850473721652 1102 84-85 0.4995965918065046 1102 86-87 0.5036200187624473 1102 88-89 0.5476885811284156 1102 90-91 0.6214405805730188 1102 92-93 0.8076043851625485 1102 94-95 0.5727131501128184 1102 96-97 0.5570753664224704 1102 98-99 0.6314691346059753 1102 100-101 0.7585772790943004 1103 1 0.22436196813383447 1103 2 0.3542186589082448 1103 3 0.2567853154577975 1103 4 0.27603093243457977 1103 5 0.2307667259380679 1103 6 0.1704479197795834 1103 7 0.22207331888616721 1103 8 0.3091213498666079 1103 9 0.32651566430336487 1103 10-11 0.3865890012281241 1103 12-13 0.30098409486310373 1103 14-15 0.4701978581522397 1103 16-17 0.5091848570510535 1103 18-19 0.3181922225108238 1103 20-21 0.5402644795900358 1103 22-23 0.5176171050509168 1103 24-25 0.3273974105482722 1103 26-27 0.4323453238209325 1103 28-29 0.306957302348529 1103 30-31 0.3697757000679758 1103 32-33 0.4669494806836596 1103 34-35 0.414136054241645 1103 36-37 0.33659540238288344 1103 38-39 0.3071169807620677 1103 40-41 0.28320670634996503 1103 42-43 0.4633315902453461 1103 44-45 0.4548025112816134 1103 46-47 0.5881279862974722 1103 48-49 0.7962860146773636 1103 50-51 0.6542383621994006 1103 52-53 0.7597902574296782 1103 54-55 0.8074928731762654 1103 56-57 0.8106549276181383 1103 58-59 0.9729998387571399 1103 60-61 0.9364732253326977 1103 62-63 0.9603176495269494 1103 64-65 0.9907528553878464 1103 66-67 0.8566591468471998 1103 68-69 0.8502408622013391 1103 70-71 0.7384219584115854 1103 72-73 0.7470623609580258 1103 74-75 0.6304364932829536 1103 76-77 0.8666926387810641 1103 78-79 0.6863996701298625 1103 80-81 0.6601595174060897 1103 82-83 0.7605825478158899 1103 84-85 0.7394429094900694 1103 86-87 0.7744757296732203 1103 88-89 0.7767104629564443 1103 90-91 0.6894749188699656 1103 92-93 0.5940505734004375 1103 94-95 0.7883079116940763 1103 96-97 0.8790148788743473 1103 98-99 1.0254273417068234 1103 100-101 0.9428832146223982 1104 1 0.30471620607252703 1104 2 0.28844673032948975 1104 3 0.3228064785801834 1104 4 0.23145832790574872 1104 5 0.2893614422585671 1104 6 0.3135503189753521 1104 7 0.31142088597179907 1104 8 0.41467148290772826 1104 9 0.3586219004115563 1104 10-11 0.5799260846827039 1104 12-13 0.4587166081822929 1104 14-15 0.6565264383925395 1104 16-17 0.6471591510837698 1104 18-19 0.47573815801523267 1104 20-21 0.5504900577375906 1104 22-23 0.5822280276503662 1104 24-25 0.6203610134865514 1104 26-27 0.2914695591187595 1104 28-29 0.3777199554108037 1104 30-31 0.5710564964303586 1104 32-33 0.675036654294324 1104 34-35 0.45006342256156984 1104 36-37 0.4094967384563972 1104 38-39 0.5864079572184551 1104 40-41 0.589756608721494 1104 42-43 0.5880809053144773 1104 44-45 0.7441292392033532 1104 46-47 0.9028548238452814 1104 48-49 1.1006250627684864 1104 50-51 0.8994967562285439 1104 52-53 0.7550160467536244 1104 54-55 0.9124651719402621 1104 56-57 0.8837330949041728 1104 58-59 0.9430972630086316 1104 60-61 1.1728763670803133 1104 62-63 1.1209769282690942 1104 64-65 1.1344741638274272 1104 66-67 0.9314733696461772 1104 68-69 0.9016785556539801 1104 70-71 0.7988973048452834 1104 72-73 1.0461575049687184 1104 74-75 1.0286121149885439 1104 76-77 0.8956409757554518 1104 78-79 0.9620066629920991 1104 80-81 1.0553747247047163 1104 82-83 0.9543598528061032 1104 84-85 0.8020516015099695 1104 86-87 0.9305937879458384 1104 88-89 1.099850706816433 1104 90-91 1.2088500038626542 1104 92-93 1.151360594717648 1104 94-95 1.2122315039018048 1104 96-97 1.2095878352091027 1104 98-99 1.2177749129695705 1104 100-101 1.2048526223585085 1105 1 0.2710875738967644 1105 2 0.33962497271078007 1105 3 0.36644574151426923 1105 4 0.3130051032423893 1105 5 0.43943231398074545 1105 6 0.34742700217874756 1105 7 0.4499485259434479 1105 8 0.3847901930423845 1105 9 0.32282282174394794 1105 10-11 0.41995531926810514 1105 12-13 0.4372646237740767 1105 14-15 0.45060422010763546 1105 16-17 0.42059997319574904 1105 18-19 0.3771910283199844 1105 20-21 0.39152921436409827 1105 22-23 0.3987544273668746 1105 24-25 0.2437929766332516 1105 26-27 0.1800060580556888 1105 28-29 0.35535549757947393 1105 30-31 0.12236319380810556 1105 32-33 0.22318070106965848 1105 34-35 0.22390289811082198 1105 36-37 0.20384471861231646 1105 38-39 -5.162100388105273E-4 1105 40-41 0.02693768597167434 1105 42-43 0.17520439929037224 1105 44-45 0.245241039345089 1105 46-47 0.32020067784953454 1105 48-49 0.3469042973538876 1105 50-51 0.18042340399608747 1105 52-53 0.0925204762362597 1105 54-55 0.12964270560433278 1105 56-57 0.2984079708786638 1105 58-59 0.1659755408257837 1105 60-61 0.19726066899384875 1105 62-63 0.3913348304661213 1105 64-65 0.3933552765134678 1105 66-67 0.22115267510329062 1105 68-69 0.24758571369792293 1105 70-71 0.40059379669502704 1105 72-73 0.5850084971728293 1105 74-75 0.4686014842302235 1105 76-77 0.48384083401200684 1105 78-79 0.7265442250048579 1105 80-81 0.7363500967812548 1105 82-83 0.6624658060307702 1105 84-85 0.5741945852094723 1105 86-87 0.5721157581797129 1105 88-89 0.506127283712253 1105 90-91 0.5990209818924228 1105 92-93 0.6155792339805792 1105 94-95 0.7490050687494332 1105 96-97 0.6804329804961355 1105 98-99 0.8708932687456148 1105 100-101 0.7769424522663719 1106 1 0.23684151238138895 1106 2 0.18653872320722797 1106 3 0.11977414714508683 1106 4 0.13501483361616096 1106 5 -0.0573352602657522 1106 6 0.2602375577588205 1106 7 0.05924037096352919 1106 8 0.15313834625715828 1106 9 0.09429749188206671 1106 10-11 0.3212187785152665 1106 12-13 0.10833303425905427 1106 14-15 0.2252387794827726 1106 16-17 0.3502114201751425 1106 18-19 0.2104088916093403 1106 20-21 0.34258416560191307 1106 22-23 0.004600098343438219 1106 24-25 0.1405302124127914 1106 26-27 0.08896969602400162 1106 28-29 0.04190921321426089 1106 30-31 0.24914681986167864 1106 32-33 0.2933621251021421 1106 34-35 0.27979131874539576 1106 36-37 0.2523388274336469 1106 38-39 0.31704038499230336 1106 40-41 0.374484683015055 1106 42-43 0.29836230273682673 1106 44-45 0.44221463936274574 1106 46-47 0.4528901002051242 1106 48-49 0.7410175315447844 1106 50-51 0.7950250323443484 1106 52-53 0.7450719413084173 1106 54-55 0.7562892569480439 1106 56-57 0.6393662803089555 1106 58-59 0.8162924399034992 1106 60-61 0.8643166793199626 1106 62-63 0.935806610175753 1106 64-65 0.874204808749468 1106 66-67 0.8834860893392076 1106 68-69 0.8612689139097469 1106 70-71 0.8256387527869933 1106 72-73 1.0341223381330664 1106 74-75 0.9377301634588484 1106 76-77 1.0948928617248157 1106 78-79 0.9119668674301664 1106 80-81 0.9479782795622462 1106 82-83 0.9948314342025206 1106 84-85 1.2165953508420593 1106 86-87 1.0924894177710804 1106 88-89 1.0337432869777743 1106 90-91 0.8458671603488028 1106 92-93 1.0041813477443178 1106 94-95 1.0534041464600357 1106 96-97 1.047639304135867 1106 98-99 1.0616372782774093 1106 100-101 0.690360604598574 1107 1 0.2543956738210653 1107 2 0.23180836764331758 1107 3 0.29254784776176734 1107 4 0.2762153494907764 1107 5 0.26250529790558375 1107 6 0.23523035970647044 1107 7 0.346863185069779 1107 8 0.40941002204893095 1107 9 0.34283161632915693 1107 10-11 0.3948476134302794 1107 12-13 0.4352833373155107 1107 14-15 0.532750933827181 1107 16-17 0.45825972293970096 1107 18-19 0.5015776338327456 1107 20-21 0.566761046309594 1107 22-23 0.41227109254922567 1107 24-25 0.43380555622982087 1107 26-27 0.25338740908208734 1107 28-29 0.2761048741658456 1107 30-31 0.37327020498501895 1107 32-33 0.24304560956144172 1107 34-35 0.28025670761342525 1107 36-37 0.3276023442969631 1107 38-39 0.06748263219301265 1107 40-41 0.3547736613913699 1107 42-43 0.22880043838731723 1107 44-45 0.3064199033963888 1107 46-47 0.4540954545338636 1107 48-49 0.509793374256418 1107 50-51 0.2033800544836808 1107 52-53 0.3788830246621444 1107 54-55 0.49915722223611425 1107 56-57 0.6606727992172594 1107 58-59 0.7586529354020897 1107 60-61 0.8725414834392353 1107 62-63 0.7093726360501265 1107 64-65 0.743943344821794 1107 66-67 0.6947060214986251 1107 68-69 0.8025117946526379 1107 70-71 0.40724734569979404 1107 72-73 0.6631642503900892 1107 74-75 0.7879328731067368 1107 76-77 0.9043489161244871 1107 78-79 0.8219003500438191 1107 80-81 0.7836463184493319 1107 82-83 0.8550020976102317 1107 84-85 0.6543664135926086 1107 86-87 0.5340179490333412 1107 88-89 0.3803015389556066 1107 90-91 0.5420857835132971 1107 92-93 0.43882643975161173 1107 94-95 0.4816513483059168 1107 96-97 0.5202696683885506 1107 98-99 0.5958409465298224 1107 100-101 0.848405414817023 1108 1 0.19305019071919816 1108 2 0.2272486670483289 1108 3 0.17815089086372637 1108 4 0.19321629987057065 1108 5 0.057537499980384155 1108 6 -0.00246304729898128 1108 7 0.09925446327164877 1108 8 0.11479523244132395 1108 9 0.13670178454402304 1108 10-11 0.008356684487090149 1108 12-13 0.12044792045723085 1108 14-15 0.3732757887350786 1108 16-17 0.3858006328988779 1108 18-19 0.4585956462419176 1108 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-1.3870660008691829 2316 70-71 -1.2676962247930135 2316 72-73 -1.3793845774086044 2316 74-75 -1.7490254295489898 2316 76-77 -2.143435555996053 2316 78-79 -2.2472213314460348 2316 80-81 -2.192785792734366 2316 82-83 -1.9834839208919668 2316 84-85 -2.1337587902046202 2316 86-87 -2.559817494573444 2316 88-89 -2.529786294047618 2316 90-91 -2.1198297433291735 2316 92-93 -2.2861118305730947 2316 94-95 -2.709528595857968 2316 96-97 -2.7111094583972317 2316 98-99 -2.594788529087676 2316 100-101 -2.8173279613785382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2740.0 3 915.0 4 952.0 5 969.0 6 1193.0 7 1403.0 8 1742.0 9 2103.0 10 2661.0 11 3339.0 12 4581.0 13 5980.0 14 6645.0 15 8523.0 16 11219.0 17 15248.0 18 13384.0 19 7912.0 20 7132.0 21 7421.0 22 7829.0 23 8694.0 24 9533.0 25 10901.0 26 12531.0 27 14727.0 28 17549.0 29 21358.0 30 25888.0 31 32697.0 32 41537.0 33 54427.0 34 75398.0 35 109209.0 36 165244.0 37 243489.0 38 219284.0 39 38907.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.59245872573076 18.290899123438813 14.5422398117974 21.574402339033025 2 15.403895938198387 20.265875351379385 40.94752048598392 23.382708224438307 3 19.600205098998124 25.754738799183226 30.09062379889105 24.554432302927598 4 12.522420703353617 16.61440999903691 37.67460161322054 33.188567684388936 5 13.328662185438533 37.677473430444905 33.68907610073871 15.30478828337785 6 30.630063947452268 35.86351290269729 18.163398213884342 15.343024935966099 7 27.79241031180608 33.83274231435184 20.596195028853856 17.77865234498822 8 26.70173578120034 33.38385942494117 19.98725458069382 19.92715021316467 9 27.255893836028104 16.581286936819 20.514847180986916 35.64797204616598 10-11 24.67699870111959 26.71263866217376 27.103579022772607 21.506783613934044 12-13 26.651069425152592 24.44490918716772 27.672039659753434 21.231981727926254 14-15 23.423113006958346 25.011886781035 27.369730142283228 24.19527006972343 16-17 21.373197115384617 28.617376844046365 27.646494665437306 22.362931375131716 18-19 21.195135001646364 26.60874217978268 31.173649983536382 21.022472835034574 20-21 22.55017456855922 25.388385201723214 30.98762748938223 21.07381274033534 22-23 22.03339749121687 25.630683518090752 30.905292840202424 21.43062615048995 24-25 21.772501816703468 25.55931012177468 31.29075238270021 21.37743567882164 26-27 21.74549931366385 25.68180995236794 31.159990388002118 21.412700345966087 28-29 21.274602226879328 25.872051755049036 31.531875548562123 21.321470469509514 30-31 21.436501006862464 25.788599206195723 31.319388953397947 21.45551083354387 32-33 21.2129241646725 25.96834955546851 31.099059258795997 21.719667021062993 34-35 21.78003129560771 25.75983192703342 30.995160002188964 21.4649767751699 36-37 21.90580697613946 25.504144078312873 31.043351535678905 21.546697409868763 38-39 21.795227861762616 25.58699209471367 31.110653024462238 21.50712701906148 40-41 21.680214349077612 25.771222188026144 31.04473779926828 21.50382566362797 42-43 21.524866815773752 25.9348816771091 30.681580666768703 21.85867084034844 44-45 21.689776700379255 26.21937622246195 30.23386514799555 21.85698192916324 46-47 21.95109006018495 26.297629960485626 29.654459067816276 22.09682091151315 48-49 22.039716887473755 26.440402657337653 29.337438846221414 22.182441608967178 50-51 22.102697031413935 26.751383488654362 29.217470015840686 21.928449464091013 52-53 22.171559763255182 26.899214759315605 28.993628791198393 21.935596686230824 54-55 21.778779886686717 26.680340515875788 29.16915936686101 22.371720230576482 56-57 22.03916411159389 27.21093866055891 28.648349699378596 22.101547528468608 58-59 22.197187363833525 26.651577175505647 28.797241923000804 22.35399353766002 60-61 22.062517874153613 26.413668095227315 29.449733250760744 22.074080779858324 62-63 22.204428212210853 26.440275836415623 29.34611265117079 22.009183300202732 64-65 22.02367457478714 26.656547498303755 29.202619682344793 22.11715824456431 66-67 21.905286248446146 26.946333482700744 28.9780594498753 22.17032081897781 68-69 21.7916952155207 27.06534241135452 29.069091313123828 22.073871060000947 70-71 21.838503428121374 26.97089203577986 28.757621819492602 22.432982716606165 72-73 21.75844695797589 26.981833773347642 28.647441552712838 22.61227771596363 74-75 21.806786539176105 27.335606678152725 28.516171349353737 22.341435433317436 76-77 22.025523670628438 27.036638631670517 28.53339303012521 22.404444667575834 78-79 21.997831083820174 27.00662815845846 28.541949184804416 22.453591572916945 80-81 21.918114330794598 27.350903249409765 28.438168866918605 22.292813552877035 82-83 21.90909831673609 27.15264111358307 28.52013356807338 22.41812700160746 84-85 21.904902989527393 26.997428142295743 28.377507561261652 22.72016130691521 86-87 22.163724112048616 26.811868541386158 28.50361797597453 22.520789370590695 88-89 22.16586137453532 26.779851977677318 28.33805315997949 22.71623348780787 90-91 21.78424909781459 26.87479682825764 28.748708959308043 22.592245114619725 92-93 21.88291814492615 27.041685374253944 28.621229898174484 22.45416658264542 94-95 21.74408278788567 27.089881380050485 28.337230269855652 22.828805562208192 96-97 21.727962874096733 26.993488315431897 28.216106274379722 23.062442536091655 98-99 21.701066021900004 27.09427736656615 28.123788284049684 23.080868327484165 100-101 21.89959266509002 26.87228949388792 28.378831757714973 22.849286083307092 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 627.0 1 479.5 2 435.5 3 541.5 4 455.0 5 442.5 6 782.5 7 1776.0 8 3132.0 9 3672.5 10 3015.0 11 2313.5 12 2210.0 13 2298.0 14 2409.5 15 2513.5 16 2614.0 17 2705.0 18 2805.0 19 2885.0 20 3095.5 21 3483.5 22 3997.5 23 4712.5 24 5490.5 25 6492.5 26 7933.5 27 9622.0 28 11044.0 29 12450.5 30 14404.0 31 16577.0 32 18823.5 33 20774.0 34 22864.0 35 24650.0 36 26836.0 37 29773.0 38 31239.0 39 31716.0 40 32888.5 41 35058.5 42 36994.0 43 38486.5 44 40947.0 45 43424.5 46 43769.5 47 45192.0 48 53474.0 49 53768.5 50 45817.0 51 42898.0 52 40904.0 53 40102.0 54 39140.5 55 36046.5 56 32538.0 57 29226.0 58 26827.0 59 24222.5 60 20698.5 61 17036.0 62 13482.5 63 10461.0 64 7650.0 65 5586.0 66 4087.5 67 3077.0 68 2219.5 69 1531.0 70 1140.5 71 796.0 72 549.5 73 461.5 74 352.5 75 238.5 76 157.5 77 82.5 78 45.5 79 23.5 80 25.0 81 17.0 82 12.0 83 11.5 84 8.0 85 6.0 86 6.0 87 4.5 88 2.5 89 2.0 90 2.0 91 1.0 92 1.5 93 2.5 94 1.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034806678932661005 2 0.035300390690571094 3 0.021476461469088708 4 0.03678152596430135 5 0.06056197563697045 6 0.05702370803861484 7 0.05883398448428515 8 0.06006826387906037 9 0.05842255801936008 10-11 0.06389453000286353 12-13 0.061960825617715695 14-15 0.057558562443017436 16-17 0.04640890524354801 18-19 0.03982608180474687 20-21 0.04731404346638317 22-23 0.025714154057816938 24-25 0.014070785100437428 26-27 0.011190799845961932 28-29 0.014441068918869994 30-31 0.010121091037156747 32-33 0.005924541094921023 34-35 0.008022816066038885 36-37 0.004607976407160795 38-39 0.0069531072572337 40-41 0.005759970508950994 42-43 0.004114264649250711 44-45 9.051382228351561E-4 46-47 0.002468558789550426 48-49 6.171396973876065E-4 50-51 0.0048548322861158375 52-53 0.0042376925887282315 54-55 0.0056776852159659795 56-57 0.01789705122424059 58-59 0.007364533722158771 60-61 0.01575763360663022 62-63 0.011807939543349537 64-65 0.008105101359023899 66-67 0.013124504231109765 68-69 0.021517604115581214 70-71 0.015387349788197656 72-73 0.014153070393422442 74-75 0.015428492434690163 76-77 0.02098274971117862 78-79 0.032214692203633065 80-81 0.025467298178861893 82-83 0.026578149634159588 84-85 0.017197626233867967 86-87 0.019748470316403408 88-89 0.016210202718047798 90-91 0.014729067444317541 92-93 0.025796439350801954 94-95 0.018678761507598226 96-97 0.03217354955714055 98-99 0.04780775522429325 100-101 0.056077427169287174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1215284.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.934836698212315 #Duplication Level Percentage of deduplicated Percentage of total 1 81.61858983524287 49.73415443148896 2 10.996566069004345 13.401479153117645 3 3.1074120433949948 5.6804893625499675 4 1.373624758931804 3.34806401480523 5 0.7109718520645604 2.1661476851289776 6 0.46423555412711565 1.6972870608143935 7 0.2971133388713494 1.2673186949490405 8 0.22562382589063762 1.0998680788697515 9 0.16381693416366003 0.8983942318497979 >10 0.9369234106125413 10.570376674594634 >50 0.0652446882382769 2.727176338870355 >100 0.03622787838670972 3.7666512459394883 >500 0.002433207380798854 1.0678525621254182 >1k 9.462473147551099E-4 1.2001397585750806 >5k 2.703563756443171E-4 1.3746007063212728 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9602 0.7901033832421065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7086 0.5830735860918106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4573 0.3762906448204699 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2227 0.1832493474776266 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1856 0.15272150378018637 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1821 0.14984151852571087 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1660 0.13659358635512356 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1254 0.10318575740320782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.228529298501421E-5 0.0 14-15 0.0 0.0 0.0 1.234279394775213E-4 0.0 16-17 0.0 0.0 0.0 1.6457058597002842E-4 0.0 18-19 4.1142646492507104E-5 0.0 0.0 1.6457058597002842E-4 0.0 20-21 8.228529298501421E-5 0.0 0.0 2.468558789550426E-4 0.0 22-23 8.228529298501421E-5 0.0 0.0 6.994249903726206E-4 0.0 24-25 2.468558789550426E-4 0.0 0.0 0.0014811352737302557 0.0 26-27 2.468558789550426E-4 0.0 0.0 0.002509701436042933 0.0 28-29 2.8799852544754976E-4 0.0 0.0 0.004361120528205753 0.0 30-31 3.2914117194005684E-4 0.0 0.0 0.007446819015143786 0.0 32-33 3.2914117194005684E-4 0.0 0.0 0.01123194249245444 0.0 34-35 3.2914117194005684E-4 0.0 0.0 0.015140493909242613 0.0 36-37 3.2914117194005684E-4 0.0 0.0 0.020159896781328478 0.0 38-39 4.11426464925071E-4 0.0 0.0 0.027318717271024716 0.0 40-41 4.11426464925071E-4 0.0 0.0 0.04324092146362496 0.0 42-43 4.11426464925071E-4 0.0 0.0 0.05665342422018228 0.0 44-45 4.937117579100853E-4 0.0 0.0 0.07232877253382748 0.0 46-47 7.405676368651278E-4 0.0 0.0 0.10005891626977727 0.0 48-49 9.051382228351562E-4 4.1142646492507104E-5 0.0 0.11742111308961527 0.0 50-51 9.051382228351562E-4 8.228529298501421E-5 0.0 0.13943242896310656 0.0 52-53 9.051382228351562E-4 8.228529298501421E-5 0.0 0.16469401390950594 0.0 54-55 9.874235158201705E-4 8.228529298501421E-5 0.0 0.19542757083940873 0.0 56-57 0.0011519941017901988 8.228529298501421E-5 0.0 0.2694843345259215 0.0 58-59 0.001234279394775213 8.228529298501421E-5 0.0 0.3394268335631836 0.0 60-61 0.0013165646877602273 8.228529298501421E-5 0.0 0.38789287113135695 0.0 62-63 0.0013165646877602273 8.228529298501421E-5 0.0 0.44672685561564207 0.0 64-65 0.0013165646877602273 8.228529298501421E-5 0.0 0.503832848947242 0.0 66-67 0.0014811352737302557 8.228529298501421E-5 0.0 0.5937295315333699 8.228529298501421E-5 68-69 0.0014811352737302557 8.228529298501421E-5 0.0 0.7066249535088095 8.228529298501421E-5 70-71 0.0014811352737302557 8.228529298501421E-5 0.0 0.756572126350713 8.228529298501421E-5 72-73 0.0015222779202227628 8.228529298501421E-5 0.0 0.8197260887167115 8.228529298501421E-5 74-75 0.0015634205667152698 8.228529298501421E-5 0.0 0.9415083223345324 8.228529298501421E-5 76-77 0.0015634205667152698 8.228529298501421E-5 0.0 1.078184193982641 8.228529298501421E-5 78-79 0.0015634205667152698 8.228529298501421E-5 0.0 1.2178634788247027 8.228529298501421E-5 80-81 0.0015634205667152698 8.228529298501421E-5 0.0 1.33236346401335 8.228529298501421E-5 82-83 0.001645705859700284 8.228529298501421E-5 0.0 1.5064791439696399 8.228529298501421E-5 84-85 0.0016868485061927911 8.228529298501421E-5 0.0 1.7971107987927102 8.228529298501421E-5 86-87 0.0017279911526852983 8.228529298501421E-5 0.0 2.0519894938137915 8.228529298501421E-5 88-89 0.0017279911526852983 8.228529298501421E-5 0.0 2.3730667070413167 8.228529298501421E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10960 0.0 29.654581 1 GGTATCA 7455 0.0 27.789776 1 TATTCCG 180 6.668415E-9 26.40034 5 GTACATG 7605 0.0 25.367228 1 TATCAAC 13120 0.0 24.55512 2 ATCAACG 13235 0.0 24.34076 3 TACATGG 7860 0.0 24.24198 2 TCAACGC 13405 0.0 24.032074 4 CAACGCA 13500 0.0 23.901104 5 AACGCAG 13810 0.0 23.431477 6 ACATGGG 8000 0.0 22.985254 3 GAGTACT 6970 0.0 22.907125 12-13 CATGGGG 2840 0.0 22.418926 4 CCGTATA 110 0.0028702265 21.600275 9 TACCTGG 845 0.0 21.368454 2 AGTACTT 7140 0.0 21.296873 12-13 ACGCAGA 15180 0.0 21.255049 7 TACCGTG 135 3.7860667E-4 21.1194 7 GTACTTT 7510 0.0 21.068886 14-15 CGCAGAG 15245 0.0 20.947096 8 >>END_MODULE