FastQCFastQC Report
Thu 26 May 2016
SRR937014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215284
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91600.7537332837427301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73910.60817060045224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.3981785327544837No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28090.2311393879949049No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24280.19978869136761446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.15963346839092757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17120.14087242159034433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.11388284549125964No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12160.10005891626977727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG1452.3923349E-829.4929391
ATAACGC950.001226480925.00043
TACCTGG9900.024.470092
GTATCAA135100.024.3033561
GGTATCA101450.024.0275171
GAGTACT75850.023.011712-13
TAACGCA1252.2576482E-422.7975524
GTACATG83350.022.6892971
TACATGG83600.022.2730852
GCACCGT2354.654794E-922.2316556
GTACTTT79900.021.6376714-15
AGTACTT77500.021.54123512-13
ACATGGG84950.021.1922173
GTACCTG11750.020.624141
TATCAAC161150.020.3679982
ACACCGT2108.617408E-720.3549566
ATCAACG161350.020.3427493
TCAACGC163400.020.1431874
CAACGCA165200.019.9237065
AACGCAG168450.019.6238966
Produced by FastQC (version 0.11.5)