##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937014_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1215284 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.230217792713475 33.0 31.0 34.0 31.0 34.0 2 32.28239160558355 33.0 31.0 34.0 30.0 34.0 3 32.414297398797316 34.0 31.0 34.0 31.0 34.0 4 36.085090398622874 37.0 35.0 37.0 35.0 37.0 5 36.01067322535309 37.0 35.0 37.0 35.0 37.0 6 36.05932769624219 37.0 35.0 37.0 35.0 37.0 7 36.01852324230386 37.0 35.0 37.0 35.0 37.0 8 36.00474374714059 37.0 35.0 37.0 35.0 37.0 9 37.68690692875081 39.0 37.0 39.0 35.0 39.0 10-11 37.59556243643461 39.0 37.0 39.0 35.0 39.0 12-13 37.51733874551134 39.0 37.0 39.0 35.0 39.0 14-15 38.70954978424796 40.0 38.0 41.0 34.5 41.0 16-17 38.64982835287883 40.0 38.0 41.0 34.5 41.0 18-19 38.72054309939076 40.0 38.0 41.0 34.5 41.0 20-21 38.81584345716722 40.0 38.0 41.0 35.0 41.0 22-23 38.75242823899599 40.0 38.0 41.0 35.0 41.0 24-25 38.65774460949046 40.0 38.0 41.0 34.0 41.0 26-27 38.47402253300463 40.0 38.0 41.0 34.0 41.0 28-29 38.31196905414701 40.0 38.0 41.0 34.0 41.0 30-31 38.23332488537659 40.0 38.0 41.0 34.0 41.0 32-33 38.10571438445663 40.0 37.0 41.0 34.0 41.0 34-35 37.970915851767984 40.0 37.0 41.0 33.0 41.0 36-37 37.784265241704816 40.0 37.0 41.0 33.0 41.0 38-39 37.618740146336165 40.0 37.0 41.0 33.0 41.0 40-41 37.37652227792022 40.0 36.0 41.0 33.0 41.0 42-43 37.120834307042635 40.0 36.0 41.0 32.0 41.0 44-45 36.81508149535417 40.0 36.0 41.0 31.0 41.0 46-47 36.363591555554095 39.0 35.5 41.0 30.0 41.0 48-49 35.90371386441359 39.0 35.0 41.0 29.0 41.0 50-51 35.63074474772975 39.0 35.0 41.0 28.0 41.0 52-53 35.38177084533328 39.0 35.0 40.0 27.5 41.0 54-55 35.10317752887391 38.5 35.0 40.0 26.5 41.0 56-57 34.85422296352128 38.0 34.5 40.0 26.0 41.0 58-59 34.50962861355864 38.0 34.0 40.0 26.0 41.0 60-61 34.17311385651419 37.5 34.0 40.0 25.0 41.0 62-63 33.82770735070979 37.0 33.5 40.0 23.5 41.0 64-65 33.403357157668495 36.5 33.0 39.0 22.5 41.0 66-67 32.9766046454985 36.0 32.5 39.0 22.0 40.0 68-69 32.53216943529249 35.5 32.0 38.5 20.5 40.0 70-71 32.14018410511453 35.0 32.0 38.0 20.0 40.0 72-73 31.768080958854064 35.0 31.0 37.0 18.5 39.5 74-75 31.298501008817695 35.0 31.0 37.0 17.0 39.0 76-77 29.845306529173428 33.5 29.5 35.5 13.0 37.5 78-79 30.53417349360314 34.0 30.5 36.0 12.0 38.0 80-81 30.226592302704553 34.0 30.0 35.5 7.0 37.0 82-83 30.05842338087229 34.0 30.0 35.0 7.0 37.0 84-85 29.760952172496307 34.0 30.0 35.0 4.0 36.0 86-87 29.392017832868696 34.0 30.0 35.0 2.0 36.0 88-89 29.019444426158824 34.0 29.0 35.0 2.0 35.5 90-91 28.578445861214334 34.0 29.0 35.0 2.0 35.0 92-93 28.19062005259676 34.0 29.0 35.0 2.0 35.0 94-95 27.730259758212895 33.0 27.5 35.0 2.0 35.0 96-97 27.11974649546937 33.0 26.5 35.0 2.0 35.0 98-99 26.3825118243966 33.0 25.0 35.0 2.0 35.0 100-101 23.28000368638113 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15603081976244937 1101 2 0.09142536647174637 1101 3 0.13424469899139524 1101 4 0.13201991376138267 1101 5 0.169244069924801 1101 6 0.15460092189993446 1101 7 0.11463993611974388 1101 8 0.13884738534297725 1101 9 0.13894193726063264 1101 10-11 0.09517899182744571 1101 12-13 0.15596276669874243 1101 14-15 0.21004286506887127 1101 16-17 0.1951608877861375 1101 18-19 0.10418076708956647 1101 20-21 0.1511756658949679 1101 22-23 0.20971613059378313 1101 24-25 0.3226624827951312 1101 26-27 0.11433747419564355 1101 28-29 0.16773970711179942 1101 30-31 0.11030246685955092 1101 32-33 0.2349740110675782 1101 34-35 0.2287553742008086 1101 36-37 0.2950138799233244 1101 38-39 0.25022190320945015 1101 40-41 0.25233652149010055 1101 42-43 0.3295211724058831 1101 44-45 0.23830181186665556 1101 46-47 0.4152427586419236 1101 48-49 0.3815853441026533 1101 50-51 0.18990669431759244 1101 52-53 0.289871853209533 1101 54-55 0.2557159899718613 1101 56-57 0.32104863899218117 1101 58-59 0.32470263952580325 1101 60-61 0.18388698785683033 1101 62-63 0.09586427446183876 1101 64-65 0.17200270423491304 1101 66-67 0.05737747101082391 1101 68-69 0.09634414802400926 1101 70-71 0.10563294235099363 1101 72-73 0.0878680393097433 1101 74-75 0.13216378600204948 1101 76-77 0.06255569691490237 1101 78-79 0.08867321834830832 1101 80-81 0.3775298968105609 1101 82-83 0.22628974621737186 1101 84-85 0.18323187464300972 1101 86-87 0.09910831208087245 1101 88-89 -0.04057722453776336 1101 90-91 -0.06743953757841936 1101 92-93 0.11149142514642207 1101 94-95 0.3566306277672382 1101 96-97 0.34594091523763026 1101 98-99 0.31915790830715807 1101 100-101 0.7079018497079126 1102 1 0.19824407629407403 1102 2 0.21371346623342902 1102 3 0.09371788611594667 1102 4 0.13371871231613852 1102 5 0.20617835284648578 1102 6 0.21701306484336413 1102 7 0.1442999293108187 1102 8 0.11588237441575444 1102 9 0.29368910112502533 1102 10-11 0.2959395731116956 1102 12-13 0.20492797530433648 1102 14-15 0.20975012261084913 1102 16-17 0.2175225678327095 1102 18-19 0.2777053176458111 1102 20-21 0.19553867477951314 1102 22-23 0.3054724288465209 1102 24-25 0.2626018888814272 1102 26-27 0.17106396145087643 1102 28-29 0.2368536961516341 1102 30-31 0.5110710101895535 1102 32-33 0.3641647240275958 1102 34-35 0.3449967806824574 1102 36-37 0.4994984335545638 1102 38-39 0.5579312912850583 1102 40-41 0.6543920334221909 1102 42-43 0.526756489295316 1102 44-45 0.5973574720575243 1102 46-47 0.6369682300499449 1102 48-49 0.7673553837481535 1102 50-51 0.6532349529907364 1102 52-53 0.5003128407228488 1102 54-55 0.6122086950906294 1102 56-57 0.8324638788968528 1102 58-59 0.6457052120902418 1102 60-61 0.5780703702436867 1102 62-63 0.5513355040213455 1102 64-65 0.6366024878869112 1102 66-67 0.6550463762020797 1102 68-69 0.5466249474133988 1102 70-71 0.4804747310108368 1102 72-73 0.5659713839180718 1102 74-75 0.7137466132421331 1102 76-77 0.5819576923682988 1102 78-79 0.6490025313061274 1102 80-81 0.8120914900982825 1102 82-83 0.835759638702303 1102 84-85 0.6663489057334253 1102 86-87 0.5862200937121571 1102 88-89 0.4065897972178405 1102 90-91 0.5746665058888567 1102 92-93 0.6539068215464781 1102 94-95 0.7235807249591062 1102 96-97 0.6762800160206588 1102 98-99 0.8113973160407788 1102 100-101 0.9973184376693034 1103 1 0.16211519062857604 1103 2 0.11942047527320909 1103 3 0.0335134047861132 1103 4 0.05186535927523295 1103 5 0.08866049668726106 1103 6 0.11834171915914027 1103 7 0.1869297881140426 1103 8 0.17554137838786943 1103 9 0.21246844061543868 1103 10-11 0.06961447625695172 1103 12-13 0.19149148325163168 1103 14-15 0.2843850812065938 1103 16-17 0.20681384509127554 1103 18-19 0.2814593953377056 1103 20-21 0.23115880526565746 1103 22-23 0.15299792780911758 1103 24-25 0.034787822451015415 1103 26-27 0.10286035686305439 1103 28-29 0.090362423789351 1103 30-31 0.16971598475943495 1103 32-33 0.11624201350240071 1103 34-35 0.14192332561511023 1103 36-37 0.15692794227302187 1103 38-39 -0.014822803237834137 1103 40-41 0.07693826511465574 1103 42-43 -0.1447225040885769 1103 44-45 -0.12633969390836342 1103 46-47 -0.2968693064427157 1103 48-49 -0.20202329229491056 1103 50-51 -0.17310223158106197 1103 52-53 -0.1420613399135391 1103 54-55 -0.26247094215544564 1103 56-57 -0.19892555062410366 1103 58-59 -0.1052168588570126 1103 60-61 -0.2907260680110326 1103 62-63 -0.25575199115886704 1103 64-65 -0.38523994030592945 1103 66-67 -0.4189882130297633 1103 68-69 -0.22186983344699485 1103 70-71 -0.2982701278841873 1103 72-73 -0.3685669341449618 1103 74-75 -0.3692910300508707 1103 76-77 -0.21300670241960162 1103 78-79 -0.1652339304507855 1103 80-81 -0.029290885704252645 1103 82-83 -0.12863361910575222 1103 84-85 -0.17036095539553386 1103 86-87 -0.13950922405096833 1103 88-89 -0.1632844752239997 1103 90-91 -0.1736917067203123 1103 92-93 -0.07515905039104354 1103 94-95 -0.09544855485777504 1103 96-97 -0.07380987681178297 1103 98-99 0.007276666853865521 1103 100-101 0.38919132231881903 1104 1 0.11036769446107542 1104 2 0.14670864099515057 1104 3 0.13184455441641774 1104 4 0.1276735034432619 1104 5 -0.027450776093687068 1104 6 0.06358530763531434 1104 7 -0.07347993119773832 1104 8 0.11354509787694411 1104 9 0.07722690820822464 1104 10-11 0.020280656521755702 1104 12-13 0.11850173157184685 1104 14-15 0.08349020388150308 1104 16-17 0.12755563578345885 1104 18-19 0.021364119979942586 1104 20-21 0.023550143413807234 1104 22-23 0.007269515488857792 1104 24-25 0.12625620377707492 1104 26-27 0.14391350972643835 1104 28-29 0.04094761443057138 1104 30-31 0.15919104668517292 1104 32-33 0.10263668498853207 1104 34-35 0.07605950050071186 1104 36-37 0.0018481292035730235 1104 38-39 -0.02638018496629968 1104 40-41 0.19129190062050583 1104 42-43 0.13362769318729306 1104 44-45 0.11330837020757656 1104 46-47 -0.08651883254898962 1104 48-49 0.014501886195546376 1104 50-51 0.14664433819510236 1104 52-53 0.05751774610578764 1104 54-55 -0.10419285117138344 1104 56-57 -0.13685222554288856 1104 58-59 -0.09616296153885884 1104 60-61 -0.12376769946229871 1104 62-63 -0.10846760340561445 1104 64-65 0.012973971622841418 1104 66-67 -0.14144373487444284 1104 68-69 -0.052436292046913024 1104 70-71 -0.17496098933066406 1104 72-73 -0.12972667770721458 1104 74-75 0.1272090114882225 1104 76-77 0.16504195354715634 1104 78-79 0.3015661254297335 1104 80-81 0.22093108155923247 1104 82-83 -0.013719089845753984 1104 84-85 0.017495520780752116 1104 86-87 0.10398480290627532 1104 88-89 -0.05861640968784698 1104 90-91 0.028407804232315925 1104 92-93 0.19402075446341271 1104 94-95 0.19096069011936123 1104 96-97 0.5605194200358419 1104 98-99 0.7493326058531302 1104 100-101 0.48745331748543563 1105 1 0.20941092621161062 1105 2 0.10435392802562404 1105 3 0.12620139353760607 1105 4 0.047118932217181 1105 5 0.2203344825881004 1105 6 0.1336774408741519 1105 7 0.22404841749114013 1105 8 0.14767869107325993 1105 9 0.01106815555053231 1105 10-11 0.1047287429852517 1105 12-13 0.20902706821252792 1105 14-15 0.2911753562557138 1105 16-17 0.21796580587558623 1105 18-19 0.2755544105540082 1105 20-21 0.3347257281055178 1105 22-23 0.022312038522166233 1105 24-25 0.21786233418104217 1105 26-27 0.21635326876258176 1105 28-29 0.031304630731064265 1105 30-31 0.18474473201472108 1105 32-33 0.18882112864550749 1105 34-35 0.16016651680120475 1105 36-37 0.13254887335665444 1105 38-39 0.37161325939940326 1105 40-41 0.4717401642633092 1105 42-43 0.5013544826982823 1105 44-45 0.43914554242586235 1105 46-47 0.44155522840068784 1105 48-49 0.27504848435288665 1105 50-51 0.31574869680601836 1105 52-53 0.2901630331362668 1105 54-55 0.18248769454087466 1105 56-57 0.1981318283196174 1105 58-59 0.3634312978516405 1105 60-61 0.2574202877807963 1105 62-63 0.4765828926964346 1105 64-65 0.47692684192759316 1105 66-67 0.39777933387112085 1105 68-69 0.5564758270175787 1105 70-71 0.5903118667997376 1105 72-73 0.5200429891956908 1105 74-75 0.42929086832733887 1105 76-77 0.4521920244188742 1105 78-79 0.7335957143737453 1105 80-81 0.6527834911977877 1105 82-83 0.7134912219898233 1105 84-85 0.5145587737928032 1105 86-87 0.5253659155923138 1105 88-89 0.3997110176686398 1105 90-91 0.47508569225499286 1105 92-93 0.5560955241303169 1105 94-95 0.35900197289753066 1105 96-97 0.9073479104681574 1105 98-99 1.1585592181848128 1105 100-101 1.2240364145796931 1106 1 -0.007007148895404214 1106 2 0.0167204387709603 1106 3 -0.03635376731143225 1106 4 -0.01488020727357764 1106 5 0.00589668196147386 1106 6 0.018329486238435777 1106 7 0.002933396088728557 1106 8 0.05524951760342134 1106 9 0.11035106451306831 1106 10-11 0.11494039516300347 1106 12-13 -0.021696145679179324 1106 14-15 0.0991226555805369 1106 16-17 0.024268208682173054 1106 18-19 0.052018931095901166 1106 20-21 0.018703432090340755 1106 22-23 0.1159100703830731 1106 24-25 0.016582878287657365 1106 26-27 0.028162654932650355 1106 28-29 -0.01674957031919888 1106 30-31 -0.0627065609555757 1106 32-33 -0.09344989867013709 1106 34-35 -0.08324396817297242 1106 36-37 -0.0027819262550181634 1106 38-39 -0.025032133566483594 1106 40-41 0.1428673210525062 1106 42-43 0.11113615605779614 1106 44-45 0.05896842093960686 1106 46-47 0.14388577427244797 1106 48-49 0.07290300312963183 1106 50-51 -0.03353172991831599 1106 52-53 -0.06331375517846283 1106 54-55 0.09443139737835793 1106 56-57 0.10331970617143327 1106 58-59 0.2441465134202403 1106 60-61 0.008742217527931473 1106 62-63 0.06488686207114114 1106 64-65 0.10923510281634918 1106 66-67 0.02416800824559573 1106 68-69 0.22372683094503287 1106 70-71 0.167369366468769 1106 72-73 0.33893557999045143 1106 74-75 0.18338987101919102 1106 76-77 -0.03712800779505798 1106 78-79 0.09683404233809156 1106 80-81 0.4097709917493937 1106 82-83 0.39990593228504423 1106 84-85 0.3208871021984301 1106 86-87 0.3627128442362384 1106 88-89 0.08519709507034179 1106 90-91 0.12829439884340488 1106 92-93 0.3732698544776092 1106 94-95 0.2627333285305582 1106 96-97 0.5147736639653537 1106 98-99 0.39598337201738687 1106 100-101 0.4369294356290716 1107 1 0.08649824865004518 1107 2 0.0360049722434681 1107 3 -0.023930525240501765 1107 4 0.06592765555511448 1107 5 0.11427067174960825 1107 6 0.11442731841790277 1107 7 0.09462607701823345 1107 8 0.07839572807731088 1107 9 0.07200461188931229 1107 10-11 0.06451995507947572 1107 12-13 0.1523189705147061 1107 14-15 0.1199177862330032 1107 16-17 0.12064175324166371 1107 18-19 0.16851039309841553 1107 20-21 0.30626661736054217 1107 22-23 0.12374700312118136 1107 24-25 0.19380886532946562 1107 26-27 0.23219521114135233 1107 28-29 0.15236348765763807 1107 30-31 0.18179381470609712 1107 32-33 0.1821364089590034 1107 34-35 0.2541138383490562 1107 36-37 0.19709194455939638 1107 38-39 0.2494862500036703 1107 40-41 0.28412661392265903 1107 42-43 0.3053671513539342 1107 44-45 0.3346717146661433 1107 46-47 0.40477215408087375 1107 48-49 0.4827212033269248 1107 50-51 0.48929731193715753 1107 52-53 0.6821304597738376 1107 54-55 0.564064986699222 1107 56-57 0.6087215108239619 1107 58-59 0.4682688763349674 1107 60-61 0.4312917421157465 1107 62-63 0.5991095547617178 1107 64-65 0.5478974746924266 1107 66-67 0.3871131162106991 1107 68-69 0.4480808944798369 1107 70-71 0.4606819073216215 1107 72-73 0.5355270253536837 1107 74-75 0.5969485029178934 1107 76-77 0.5967435562352357 1107 78-79 0.6638721871884599 1107 80-81 0.6646714882588363 1107 82-83 0.6749161117791971 1107 84-85 0.7000969690103283 1107 86-87 0.8042274621034231 1107 88-89 0.6442049393856983 1107 90-91 0.5625155582210652 1107 92-93 0.6840973721064465 1107 94-95 0.6891609990363747 1107 96-97 0.5498814189510348 1107 98-99 0.8233605607506043 1107 100-101 0.7469755315588067 1108 1 0.11004314171463392 1108 2 0.061260195205392165 1108 3 0.016615082377626322 1108 4 0.028773229450095528 1108 5 -0.03019236366441902 1108 6 0.1038900118838626 1108 7 0.019689337410504493 1108 8 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2316 72-73 -0.8673842648109336 2316 74-75 -0.8864726288523492 2316 76-77 -0.8862184196674328 2316 78-79 -0.9671188751150162 2316 80-81 -0.7884629274610013 2316 82-83 -0.9405316314437862 2316 84-85 -1.272733620333799 2316 86-87 -1.0184657837540954 2316 88-89 -0.9720366768429578 2316 90-91 -1.044744865538899 2316 92-93 -1.0537050399309251 2316 94-95 -1.228362048053313 2316 96-97 -1.057387070282136 2316 98-99 -1.1612058682150455 2316 100-101 -1.4930650492935271 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 8.0 5 30.0 6 74.0 7 150.0 8 372.0 9 631.0 10 1234.0 11 1991.0 12 3148.0 13 4391.0 14 5553.0 15 7953.0 16 10994.0 17 15919.0 18 14615.0 19 8313.0 20 6549.0 21 6657.0 22 6574.0 23 7050.0 24 8094.0 25 9373.0 26 11629.0 27 14337.0 28 17592.0 29 22305.0 30 27759.0 31 34264.0 32 44343.0 33 58472.0 34 81350.0 35 117161.0 36 180661.0 37 271814.0 38 199125.0 39 14795.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66923187719912 19.277104467170105 14.841704561822262 21.211959093808513 2 15.78013571584926 21.253042744073745 39.70338697121264 23.263434568864355 3 19.98605964398602 26.00049210937995 30.015462767975364 23.997985478658666 4 12.930074723336046 17.390681841184318 36.87404497553245 32.805198459947185 5 13.347037118716035 38.54140265759079 32.97927316175623 15.13228706193694 6 30.83435641380945 36.550057435134505 17.36795679034695 15.247629360709103 7 27.975271623752146 34.33098765391464 20.012770677471277 17.68097004486194 8 27.04730260137153 33.87213831851373 19.259069088491394 19.82148999162334 9 27.465289977725355 16.778807899065484 20.053271542513144 35.702630580696024 10-11 24.931003781832064 27.30460534327779 26.344418259435653 21.419972615454494 12-13 26.862692177301767 24.834606561100124 27.287901428801824 21.014799832796285 14-15 23.52717554086123 25.525309310416333 26.83973458055895 24.10778056816349 16-17 21.500086193879863 29.090043598880428 27.19266738995469 22.21720281728502 18-19 21.308359226880135 26.997807825995366 30.80745075837371 20.88638218875079 20-21 22.664159128555077 25.824447097720753 30.66946494973541 20.841928823988763 22-23 22.15823626411604 26.109082321498512 30.543231047228463 21.18945036715698 24-25 21.958004381092508 26.11802617134976 30.907521424069017 21.016448023488714 26-27 21.88755168795902 26.215515645250882 30.838939291077782 21.057993375712318 28-29 21.349572997019457 26.343179061465715 31.196686344956525 21.110561596558302 30-31 21.49723672097199 26.315042205388444 30.99168979173243 21.19603128190714 32-33 21.46946140895693 26.349110741375252 30.77960506285784 21.401822786809973 34-35 21.833814990849604 26.246700262004136 30.764749252827407 21.154735494318857 36-37 22.13866322335559 25.98720526833179 30.68102092856652 21.193110579746104 38-39 22.0104434667438 26.002594614344275 30.82949894647795 21.157462972433972 40-41 21.814620819052244 26.322123791850622 30.66631829721401 21.19693709188313 42-43 21.699700418821152 26.58582377417973 30.23083098083314 21.483644826165975 44-45 21.88152521626431 26.71444882173238 29.846100042172825 21.55792591983049 46-47 22.271246677169167 26.823951783415406 29.0457689733075 21.859032566107928 48-49 22.394141531919132 27.068836341658393 28.79380777796475 21.743214348457727 50-51 22.43143618546678 27.418078703198923 28.599700552114754 21.550784559219544 52-53 22.48769278559629 27.618191430928395 28.3758179951738 21.518297788301513 54-55 21.922699196183878 27.473132414274904 28.491922135533347 22.112246254007868 56-57 22.310218503603327 27.83201373197308 28.062086818852826 21.795680945570766 58-59 22.450128243423052 27.334651562166997 28.210631902289784 22.004588292120168 60-61 22.24789524419611 27.21562499614287 28.759256584986137 21.77722317467488 62-63 22.423419071936333 27.16289127717577 28.724626280463067 21.68906337042483 64-65 22.219972901167438 27.348507198307416 28.61889355623008 21.81262634429507 66-67 22.0291835901757 27.6642183807411 28.359782830510373 21.946815198572832 68-69 22.02454275480203 27.72180928141717 28.383844867012765 21.869803096768035 70-71 22.10454464661892 27.62702924215294 28.180997537918685 22.08742857330946 72-73 22.0862121962149 27.66535279006408 27.947056492579048 22.301378521141974 74-75 22.00080562505683 28.020116762144344 27.77954016830898 22.19953744448984 76-77 22.297841350693595 27.71917192459848 27.88630112468653 22.096685600021395 78-79 22.253732080254128 27.76264463354017 27.714008262422436 22.269615023783267 80-81 22.342354425703014 28.105422488534547 27.532304243913856 22.019918841848575 82-83 22.048928128530765 28.031675157647516 27.712347896785 22.20704881703671 84-85 22.154414178825075 27.833695527850853 27.530883905479985 22.481006387844086 86-87 22.36925689797611 27.705046721589355 27.66106523248887 22.264631147945664 88-89 22.256178969061647 27.623333551391603 27.583301344596663 22.53718613495009 90-91 22.008222874204343 27.759421066208827 27.80200424844386 22.43035181114297 92-93 21.89706664669873 27.99277369070458 27.612943996925814 22.497215665670875 94-95 21.815094602005882 27.92119976087727 27.421930253674994 22.841775383441863 96-97 21.897920433810388 27.775590348706363 27.297757096630193 23.028732120853057 98-99 21.779727577520106 27.98857713212947 27.08068048431577 23.15101480603465 100-101 21.673898938549073 28.15373202920667 27.254096927391885 22.918272104852377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1029.0 1 712.5 2 484.0 3 581.0 4 511.5 5 492.0 6 848.0 7 1662.0 8 2628.5 9 3178.0 10 2872.0 11 2371.5 12 2306.5 13 2457.5 14 2633.5 15 2669.0 16 2782.5 17 2976.0 18 3026.5 19 3104.5 20 3288.5 21 3644.5 22 4166.0 23 4790.0 24 5624.5 25 6630.5 26 7992.5 27 9692.5 28 11077.5 29 12633.0 30 14753.0 31 16754.0 32 18676.0 33 20453.5 34 22861.5 35 24855.0 36 27076.5 37 29869.5 38 30975.5 39 31400.0 40 32595.0 41 34654.5 42 36776.0 43 38359.0 44 40540.0 45 42990.0 46 43388.0 47 45123.0 48 54019.5 49 53962.0 50 45626.5 51 42770.5 52 40786.0 53 39717.0 54 38533.0 55 35995.5 56 32339.0 57 28968.5 58 27094.0 59 24687.0 60 20871.5 61 16930.0 62 13602.5 63 10637.5 64 7637.0 65 5578.0 66 4102.0 67 3092.0 68 2260.0 69 1522.0 70 1120.0 71 839.0 72 633.5 73 486.0 74 376.0 75 281.5 76 171.5 77 71.5 78 36.5 79 29.5 80 23.0 81 12.0 82 7.5 83 6.5 84 2.5 85 0.5 86 0.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025426155532369387 2 0.008063958712531391 3 0.00880452634939652 4 8.22852929850142E-5 5 0.003949694063280682 6 0.0 7 0.0 8 1.645705859700284E-4 9 8.22852929850142E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.11426464925071E-5 18-19 0.004278835235220739 20-21 0.001234279394775213 22-23 0.0 24-25 0.005101688165070881 26-27 0.0048548322861158375 28-29 0.005759970508950994 30-31 0.027359859917517222 32-33 1.645705859700284E-4 34-35 0.0029622705474605113 36-37 0.014358783625884977 38-39 0.006130254327383558 40-41 0.008146244005516405 42-43 0.007199963136188743 44-45 0.00382626612380316 46-47 0.0014399926272377485 48-49 0.0014399926272377485 50-51 0.003908551416788175 52-53 0.003414839658878089 54-55 0.0015222779202227628 56-57 0.0014811352737302557 58-59 0.0013988499807452415 60-61 4.11426464925071E-5 62-63 8.22852929850142E-5 64-65 0.008434242530963955 66-67 9.874235158201705E-4 68-69 0.0013988499807452415 70-71 0.004278835235220739 72-73 0.01460563950484002 74-75 0.006459395499323615 76-77 0.0046491190536533025 78-79 0.011849082189842045 80-81 2.0571323246253552E-4 82-83 0.006006826387906037 84-85 5.759970508950994E-4 86-87 0.0 88-89 0.0010285661623126777 90-91 0.0012754220412677202 92-93 2.0571323246253552E-4 94-95 6.994249903726207E-4 96-97 5.348544044025923E-4 98-99 2.0571323246253552E-4 100-101 0.0011108514552976916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1215284.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.93602675630704 #Duplication Level Percentage of deduplicated Percentage of total 1 80.49539582671015 47.440788022025146 2 11.545967905594907 13.609469468232074 3 3.3530276945580932 5.928423897633428 4 1.4416342554826997 3.3985678021574866 5 0.7948933286042449 2.3423927241514866 6 0.49131848051975513 1.737381546826824 7 0.3294366420523673 1.3590980725354382 8 0.24004832180202618 1.1317995453224654 9 0.17300394621549386 0.9176548682792743 >10 1.015281415976381 11.031507598523275 >50 0.07663295275853385 3.1102114661058344 >100 0.03917667549534194 4.141915290173789 >500 0.002788369486656883 1.0590596056322206 >1k 0.0011153477946627534 1.4317723351804983 >5k 2.7883694866568835E-4 1.3599577572207995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9160 0.7537332837427301 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7391 0.60817060045224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4839 0.3981785327544837 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2809 0.2311393879949049 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2428 0.19978869136761446 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.15963346839092757 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1712 0.14087242159034433 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.11388284549125964 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1216 0.10005891626977727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.228529298501421E-5 0.0 0.0 8.228529298501421E-5 0.0 14-15 8.228529298501421E-5 0.0 0.0 1.234279394775213E-4 0.0 16-17 8.228529298501421E-5 0.0 0.0 2.0571323246253552E-4 0.0 18-19 8.228529298501421E-5 0.0 0.0 3.2914117194005684E-4 0.0 20-21 8.228529298501421E-5 0.0 0.0 4.1142646492507104E-4 0.0 22-23 8.228529298501421E-5 0.0 0.0 8.639955763426492E-4 0.0 24-25 8.228529298501421E-5 0.0 0.0 0.001645705859700284 0.0 26-27 8.228529298501421E-5 0.0 0.0 0.0027154146685054686 0.0 28-29 1.234279394775213E-4 0.0 0.0 0.004607976407160796 0.0 30-31 1.6457058597002842E-4 0.0 0.0 0.008022816066038885 0.0 32-33 1.6457058597002842E-4 0.0 0.0 0.012095938068797087 0.0 34-35 2.0571323246253552E-4 0.0 0.0 0.01604563213207777 0.0 36-37 3.2914117194005684E-4 0.0 0.0 0.02114732029714865 0.0 38-39 3.2914117194005684E-4 0.0 0.0 0.029046708423710012 0.0 40-41 3.2914117194005684E-4 0.0 0.0 0.04513348320228029 0.0 42-43 4.525691114175781E-4 0.0 0.0 0.05916312565622521 0.0 44-45 4.937117579100853E-4 0.0 0.0 0.07574361219270558 0.0 46-47 4.937117579100853E-4 0.0 0.0 0.10413203827253548 0.0 48-49 4.937117579100853E-4 0.0 0.0 0.1216588056783435 0.0 50-51 4.937117579100853E-4 0.0 0.0 0.14416383330974486 0.0 52-53 4.937117579100853E-4 0.0 0.0 0.1693019903166667 0.0 54-55 4.937117579100853E-4 0.0 0.0 0.20114639870186724 0.0 56-57 6.582823438801137E-4 0.0 0.0 0.27647858442964773 0.0 58-59 6.582823438801137E-4 0.0 0.0 0.34740850698272996 0.0 60-61 6.582823438801137E-4 0.0 0.0 0.39649168424829095 0.0 62-63 6.582823438801137E-4 0.0 0.0 0.4565599481273513 0.0 64-65 6.582823438801137E-4 0.0 0.0 0.5136659414589512 0.0 66-67 6.582823438801137E-4 0.0 0.0 0.6043031916819444 0.0 68-69 6.582823438801137E-4 0.0 0.0 0.7180626092337264 0.0 70-71 6.582823438801137E-4 0.0 0.0 0.767598355610705 0.0 72-73 7.405676368651278E-4 0.0 0.0 0.8300528929863308 0.0 74-75 8.22852929850142E-4 0.0 0.0 0.9520819824831068 0.0 76-77 8.22852929850142E-4 0.0 0.0 1.0885109982522603 0.0 78-79 8.22852929850142E-4 0.0 0.0 1.2277377139829042 0.0 80-81 9.874235158201705E-4 0.0 0.0 1.3412502756557314 0.0 82-83 9.874235158201705E-4 0.0 0.0 1.5152013850260517 0.0 84-85 0.0010285661623126775 0.0 0.0 1.806491322193002 0.0 86-87 0.0010697088088051847 0.0 0.0 2.061328874567591 0.0 88-89 0.0010697088088051847 0.0 0.0 2.3828175142600414 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACG 145 2.3923349E-8 29.492939 1 ATAACGC 95 0.0012264809 25.0004 3 TACCTGG 990 0.0 24.47009 2 GTATCAA 13510 0.0 24.303356 1 GGTATCA 10145 0.0 24.027517 1 GAGTACT 7585 0.0 23.0117 12-13 TAACGCA 125 2.2576482E-4 22.797552 4 GTACATG 8335 0.0 22.689297 1 TACATGG 8360 0.0 22.273085 2 GCACCGT 235 4.654794E-9 22.231655 6 GTACTTT 7990 0.0 21.63767 14-15 AGTACTT 7750 0.0 21.541235 12-13 ACATGGG 8495 0.0 21.192217 3 GTACCTG 1175 0.0 20.62414 1 TATCAAC 16115 0.0 20.367998 2 ACACCGT 210 8.617408E-7 20.354956 6 ATCAACG 16135 0.0 20.342749 3 TCAACGC 16340 0.0 20.143187 4 CAACGCA 16520 0.0 19.923706 5 AACGCAG 16845 0.0 19.623896 6 >>END_MODULE