FastQCFastQC Report
Thu 26 May 2016
SRR937013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271039
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97290.7654367804607096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71780.5647348350444007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46060.36238069799589157No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22600.17780728994153602No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19260.15152957541035328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.14177377720117162No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17240.13563706542442838No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.10164912327631173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG650.005832669329.242695
GTATCAA120550.028.4209561
GTACATG84500.026.9932521
GGTATCA84050.026.9116231
TACATGG85400.026.2636342
ACATGGG86150.024.8766523
CTCGCGC1650.024.46330360-61
TATCAAC143550.023.7017542
ATCAACG144950.023.374483
ATGGGAG20950.023.3627455
GAGTACT71300.023.32372512-13
TCAACGC145200.023.301514
CAACGCA147400.022.9859625
TACCTGG9200.022.7266542
AACGCAG150050.022.6397866
CATGGGA53300.022.4669444
GTATAGG3650.022.1323091
AGTACTT72150.022.06113212-13
GTACTTT76050.021.49081814-15
AATTCGC1551.0913936E-1121.45824878-79
Produced by FastQC (version 0.11.5)