FastQCFastQC Report
Thu 26 May 2016
SRR937013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271039
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93470.7353826279130695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74780.5883375726472595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.395896585391951No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27990.2202135418346723No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24410.1920476082952608No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.16521916322001134No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17220.1354797138404093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.11392254682979831No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12850.10109839273224504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC650.00584889329.2260722
GTATCAA134700.026.7649461
GGTATCA95950.025.544471
GAGTACT76850.023.63803712-13
TATCAAC157150.022.8471092
ATCAACG156800.022.8375283
TCAACGC157700.022.7373094
CAACGCA159150.022.5073935
GTACATG77900.022.4999241
TACCTGG8900.022.412132
TACATGG78500.022.2034382
AACGCAG161450.022.1867566
GTACTTT81250.022.06568314-15
AGTACTT78450.021.61220712-13
TACAACG1100.002880215421.587444
ACATGGG77950.020.9587863
GTACCTG10650.020.5163651
ACGCAGA176000.020.3255777
CGCAGAG176750.020.1049358
ACTTTTT91100.019.99345816-17
Produced by FastQC (version 0.11.5)