##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937013_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1271039 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.236061206619155 33.0 31.0 34.0 31.0 34.0 2 32.342605537674295 33.0 31.0 34.0 31.0 34.0 3 32.43865845186497 34.0 31.0 34.0 31.0 34.0 4 36.08437742665646 37.0 35.0 37.0 35.0 37.0 5 36.004600960316715 37.0 35.0 37.0 35.0 37.0 6 36.06130653740759 37.0 35.0 37.0 35.0 37.0 7 36.01304444631518 37.0 35.0 37.0 35.0 37.0 8 35.98707199385699 37.0 35.0 37.0 35.0 37.0 9 37.66442807813136 39.0 37.0 39.0 35.0 39.0 10-11 37.581568307502764 39.0 37.0 39.0 35.0 39.0 12-13 37.48588595629245 39.0 37.0 39.0 35.0 39.0 14-15 38.66628600695966 40.0 38.0 41.0 34.0 41.0 16-17 38.58964831134214 40.0 38.0 41.0 34.0 41.0 18-19 38.77735694970808 40.0 38.0 41.0 35.0 41.0 20-21 38.81411191946117 40.0 38.0 41.0 35.0 41.0 22-23 38.731826482114236 40.0 38.0 41.0 34.5 41.0 24-25 38.615563330472156 40.0 38.0 41.0 34.0 41.0 26-27 38.4219382725471 40.0 38.0 41.0 34.0 41.0 28-29 38.330221968012 40.0 38.0 41.0 34.0 41.0 30-31 38.29365385326493 40.0 38.0 41.0 34.0 41.0 32-33 38.09196137962722 40.0 37.0 41.0 34.0 41.0 34-35 37.92250906541813 40.0 37.0 41.0 33.0 41.0 36-37 37.74654357576755 40.0 37.0 41.0 33.0 41.0 38-39 37.57207213940721 40.0 37.0 41.0 33.0 41.0 40-41 37.36066202531944 40.0 36.0 41.0 33.0 41.0 42-43 37.078052679736814 40.0 36.0 41.0 32.0 41.0 44-45 36.74737163847844 39.5 36.0 41.0 31.0 41.0 46-47 36.286436136105976 39.0 35.5 41.0 30.0 41.0 48-49 35.84178652267947 39.0 35.0 41.0 28.5 41.0 50-51 35.51197366878593 39.0 35.0 40.0 27.5 41.0 52-53 35.24216880835285 39.0 35.0 40.0 27.0 41.0 54-55 34.93591227334488 38.0 34.5 40.0 26.0 41.0 56-57 34.65391384528721 38.0 34.0 40.0 25.5 41.0 58-59 34.30604843753811 38.0 34.0 40.0 25.0 41.0 60-61 33.9625459958349 37.0 33.5 40.0 24.0 41.0 62-63 33.5810903520663 37.0 33.0 39.5 23.0 41.0 64-65 33.166483876576564 36.0 33.0 39.0 22.0 40.5 66-67 32.77044213434836 36.0 32.0 39.0 21.5 40.0 68-69 32.461986610953716 35.0 32.0 38.5 20.5 40.0 70-71 31.990154118008967 35.0 31.5 38.0 18.5 40.0 72-73 31.48975287146972 35.0 31.0 37.0 17.5 39.0 74-75 30.988499172724048 34.5 30.5 36.5 14.0 39.0 76-77 29.586576808422087 33.5 28.5 35.0 11.5 37.5 78-79 30.315527296959417 34.0 30.0 36.0 9.5 37.5 80-81 30.084264920273885 34.0 30.0 35.0 7.0 37.0 82-83 29.775219328439174 34.0 30.0 35.0 6.0 37.0 84-85 29.444728682597464 34.0 29.5 35.0 2.0 36.0 86-87 29.09047912770576 34.0 29.0 35.0 2.0 36.0 88-89 28.693483834878393 34.0 29.0 35.0 2.0 35.5 90-91 28.233187573316002 33.5 29.0 35.0 2.0 35.0 92-93 27.771034563062187 33.0 27.0 35.0 2.0 35.0 94-95 27.194964119905055 33.0 26.5 35.0 2.0 35.0 96-97 26.52346112117724 33.0 25.0 34.0 2.0 35.0 98-99 25.69760565962177 32.0 24.0 34.0 2.0 35.0 100-101 22.218530273264626 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.061164882799332077 1101 2 0.019430661092791013 1101 3 0.046405912455682596 1101 4 0.09189459991305426 1101 5 0.10656216618998116 1101 6 0.15342915752815145 1101 7 0.10318893534135753 1101 8 0.15815198127806696 1101 9 0.2291881507629867 1101 10-11 0.22956990337942074 1101 12-13 0.2091558330678609 1101 14-15 0.24129568427908765 1101 16-17 0.25970092995587635 1101 18-19 0.11591047275297939 1101 20-21 0.13430595691905722 1101 22-23 0.21443347831081638 1101 24-25 0.13743316293890473 1101 26-27 0.07766218242759493 1101 28-29 0.2791069654273812 1101 30-31 0.33743215683005445 1101 32-33 0.3052255844778742 1101 34-35 0.1856200782001025 1101 36-37 0.12346316734031859 1101 38-39 0.20372419093770588 1101 40-41 0.45450290456747666 1101 42-43 0.609396240407797 1101 44-45 0.6033503501133808 1101 46-47 0.5672253627102251 1101 48-49 0.6109808034290651 1101 50-51 0.5345763767387126 1101 52-53 0.6063891890631439 1101 54-55 0.75070914409973 1101 56-57 0.5202320854281979 1101 58-59 0.3673883712362169 1101 60-61 0.5547242099698693 1101 62-63 0.4960909205895092 1101 64-65 0.4698416960649823 1101 66-67 0.21981950766175373 1101 68-69 0.2938994247018982 1101 70-71 0.2996148930005269 1101 72-73 0.29137158985692224 1101 74-75 0.3492178702721347 1101 76-77 0.0842878464903194 1101 78-79 0.19045101472174153 1101 80-81 0.45902662061089927 1101 82-83 0.41686806037346713 1101 84-85 0.04357985194787872 1101 86-87 -0.25659497312718216 1101 88-89 -0.18001781965377006 1101 90-91 0.2120179568962115 1101 92-93 0.3738656982720663 1101 94-95 0.2196159094263379 1101 96-97 0.11619988640430634 1101 98-99 0.23007530369806162 1101 100-101 0.7507426898857084 1102 1 0.1645896115767158 1102 2 0.20919174011282848 1102 3 0.21941096216914246 1102 4 0.1416632734513641 1102 5 0.15842396305585282 1102 6 0.05202417164854012 1102 7 0.11260407194294686 1102 8 0.12523515906388383 1102 9 0.1449424303381477 1102 10-11 0.21254510934333126 1102 12-13 0.16057923529805862 1102 14-15 0.21384196382005172 1102 16-17 0.1952267826649674 1102 18-19 0.2140348903403364 1102 20-21 0.25422703377330436 1102 22-23 0.2030646768198423 1102 24-25 0.22634673361636715 1102 26-27 0.2187918064633223 1102 28-29 0.2708336781835996 1102 30-31 0.30677552309671086 1102 32-33 0.2891180458142273 1102 34-35 0.26134672256291935 1102 36-37 0.21583096145334935 1102 38-39 0.2552860542021804 1102 40-41 0.2454725165203655 1102 42-43 0.4320653074931826 1102 44-45 0.3914616874610246 1102 46-47 0.4456402243133155 1102 48-49 0.5953917366856487 1102 50-51 0.5851171477604211 1102 52-53 0.40845705669909904 1102 54-55 0.4594964149461873 1102 56-57 0.4852394163468574 1102 58-59 0.5880164293478245 1102 60-61 0.40961351950988956 1102 62-63 0.566540585889264 1102 64-65 0.5549371689911666 1102 66-67 0.5267771479181178 1102 68-69 0.30564617102788816 1102 70-71 0.32768201685813736 1102 72-73 0.47791657562254386 1102 74-75 0.4017294045358071 1102 76-77 0.3193834262812061 1102 78-79 0.3147930843633304 1102 80-81 0.6018995641777884 1102 82-83 0.4027250511774021 1102 84-85 0.3942688443134621 1102 86-87 0.3312210970444589 1102 88-89 0.3296562857461751 1102 90-91 0.3639516024644607 1102 92-93 0.22723521361926302 1102 94-95 0.1330663641000278 1102 96-97 0.2563417243632209 1102 98-99 0.48881576780434344 1102 100-101 0.6491574588727111 1103 1 0.262252699451615 1103 2 0.19299965235462935 1103 3 0.19430593241071392 1103 4 0.2221715007824372 1103 5 0.17801493049663009 1103 6 0.11689463494647612 1103 7 0.16148667201296973 1103 8 0.14958164056697854 1103 9 0.1606093693041899 1103 10-11 0.23673842244870258 1103 12-13 0.24838848958677318 1103 14-15 0.2945033595488411 1103 16-17 0.3046400747644 1103 18-19 0.3157314126883435 1103 20-21 0.32300693807952285 1103 22-23 0.28805855008393877 1103 24-25 0.18252598440408718 1103 26-27 0.4531175737640538 1103 28-29 0.3542229301383273 1103 30-31 0.3055501759150161 1103 32-33 0.36163963526457366 1103 34-35 0.41323871696133097 1103 36-37 0.3244638240977693 1103 38-39 0.3096827165898617 1103 40-41 0.6272192197529449 1103 42-43 0.6542178340423703 1103 44-45 0.5805079589045974 1103 46-47 0.4097769380488714 1103 48-49 0.4920687118286011 1103 50-51 0.330835726357698 1103 52-53 0.3278685983025511 1103 54-55 0.46180123464510103 1103 56-57 0.4166710303041512 1103 58-59 0.421551555967028 1103 60-61 0.25134680227334627 1103 62-63 0.25799378929689 1103 64-65 0.2262450812329675 1103 66-67 0.19535720393398748 1103 68-69 -0.030966911012022535 1103 70-71 0.15605318035446558 1103 72-73 0.22294954329671768 1103 74-75 0.42382941620577697 1103 76-77 0.3767559318244409 1103 78-79 0.43709440307029723 1103 80-81 0.4908816507689906 1103 82-83 0.5143556381770544 1103 84-85 0.5749929661479847 1103 86-87 0.5070438301521705 1103 88-89 0.4563921942535849 1103 90-91 0.47737644783731525 1103 92-93 0.39113998663828653 1103 94-95 0.24831464277898618 1103 96-97 0.29663960042810444 1103 98-99 0.014212546891751998 1103 100-101 0.4072243278189127 1104 1 0.16049773912560283 1104 2 0.08961465524079415 1104 3 0.1418018616264476 1104 4 -0.028402716722361276 1104 5 0.03290374854764622 1104 6 0.0562300417233601 1104 7 0.034757342550825854 1104 8 0.08634189937408365 1104 9 0.04651110186443219 1104 10-11 0.08086701615428638 1104 12-13 0.0816780278866105 1104 14-15 -0.11331556162871692 1104 16-17 0.12874730223723674 1104 18-19 0.08384920266171747 1104 20-21 0.007606019676138942 1104 22-23 -0.05988408847350257 1104 24-25 -0.030745203058756942 1104 26-27 -0.1974010743047998 1104 28-29 -0.1255838260712423 1104 30-31 -0.08234171783967525 1104 32-33 -0.02071986883206023 1104 34-35 0.05146144270678121 1104 36-37 0.15054484347383834 1104 38-39 0.1588978521534372 1104 40-41 0.010564758259548057 1104 42-43 -0.08787062905349075 1104 44-45 -0.2010132651737635 1104 46-47 0.021359934663017555 1104 48-49 -0.05126941741987423 1104 50-51 0.007135146540171888 1104 52-53 0.10306826057823315 1104 54-55 0.01882495288651853 1104 56-57 0.12378305656393707 1104 58-59 0.2815045157933369 1104 60-61 0.27359869981880536 1104 62-63 0.31245312883906706 1104 64-65 0.16819742074929422 1104 66-67 0.05223273701287923 1104 68-69 0.12429883150763743 1104 70-71 0.08690498657651702 1104 72-73 0.1778728230134412 1104 74-75 0.2626948520166188 1104 76-77 0.10083472842307728 1104 78-79 0.2732016133123061 1104 80-81 0.5716817644924852 1104 82-83 0.48366537101298945 1104 84-85 0.5147233897680081 1104 86-87 0.36680682004628906 1104 88-89 0.3023498408664338 1104 90-91 0.38644245054255677 1104 92-93 0.3758881149390696 1104 94-95 0.4258624368016122 1104 96-97 0.42228252587890225 1104 98-99 0.39489376786856667 1104 100-101 0.6729147242142268 1105 1 0.11223735856656702 1105 2 0.08924589648277248 1105 3 0.14873821386481723 1105 4 0.04975846773076853 1105 5 -0.030998456629724558 1105 6 0.032701389167861805 1105 7 -0.044035341275410644 1105 8 -0.018578264354800922 1105 9 0.018775833776814466 1105 10-11 0.046063564572307314 1105 12-13 0.10220175751268812 1105 14-15 0.06770027655993971 1105 16-17 -0.06340274570140281 1105 18-19 -0.07352458965335273 1105 20-21 0.014338632700692244 1105 22-23 0.07074962345215852 1105 24-25 -0.034274224373014306 1105 26-27 -0.05626373010537833 1105 28-29 -0.1448279628069855 1105 30-31 -0.04880310879770633 1105 32-33 -0.2474424331804599 1105 34-35 -0.142271041252215 1105 36-37 -0.22324147926381954 1105 38-39 -0.055189654299844904 1105 40-41 -0.17684858343231724 1105 42-43 -0.2519069702290935 1105 44-45 -0.2529054585508632 1105 46-47 -0.2872436681100581 1105 48-49 -0.32514332036256377 1105 50-51 -0.30702196312868324 1105 52-53 -0.2208462058278471 1105 54-55 -0.3146767802796404 1105 56-57 -0.20256291290283457 1105 58-59 -0.3387541684754112 1105 60-61 -0.30584207752465176 1105 62-63 -0.3275373756229172 1105 64-65 -0.7806137932045303 1105 66-67 -0.791856521044501 1105 68-69 -0.585383851581085 1105 70-71 -0.7869730649022024 1105 72-73 -0.5316840211491076 1105 74-75 -0.4579602479170042 1105 76-77 -0.7835695418033417 1105 78-79 -0.5895542130760738 1105 80-81 -0.5452871307062743 1105 82-83 -0.3976242705534965 1105 84-85 -0.24056507177045106 1105 86-87 -0.6015960857727229 1105 88-89 -0.4086728193592464 1105 90-91 -0.5667427032298455 1105 92-93 -0.5148270004824234 1105 94-95 -0.5706624728524936 1105 96-97 -0.48027527705641404 1105 98-99 -0.6210398371170491 1105 100-101 -0.15625916397812034 1106 1 0.13851384064239625 1106 2 0.05125257152659657 1106 3 0.07969272912377079 1106 4 0.03875194875811161 1106 5 0.1015840009709379 1106 6 0.08454603032423336 1106 7 0.06563666916134281 1106 8 0.07155333565480504 1106 9 0.03287076106365561 1106 10-11 0.05545384654089247 1106 12-13 0.09367962141862307 1106 14-15 0.21437841178960326 1106 16-17 0.19243041421952256 1106 18-19 -0.07994926930727075 1106 20-21 0.10844604568101346 1106 22-23 0.059491989971292014 1106 24-25 0.06075310809059431 1106 26-27 0.00383342419699062 1106 28-29 0.20821127308457932 1106 30-31 0.2427058192734961 1106 32-33 0.21255757682409637 1106 34-35 0.19718750184344458 1106 36-37 -0.029037172099762643 1106 38-39 0.18647304389053687 1106 40-41 0.19156091129728736 1106 42-43 0.18283502211941283 1106 44-45 0.1995921303496786 1106 46-47 0.19912136434474093 1106 48-49 0.20151608632688323 1106 50-51 0.22189047094125414 1106 52-53 0.24800684116157612 1106 54-55 -0.021505093990697333 1106 56-57 0.01686414583595308 1106 58-59 0.08982876534241058 1106 60-61 0.03653954849681895 1106 62-63 0.03815763130024408 1106 64-65 -0.06542341639271143 1106 66-67 0.14868761708248712 1106 68-69 0.09886366528660773 1106 70-71 -0.0035233118147957043 1106 72-73 -0.006736395031598619 1106 74-75 0.08265677344473943 1106 76-77 0.016700881509692067 1106 78-79 0.2233177065192642 1106 80-81 0.5244068997716198 1106 82-83 0.5513477106699405 1106 84-85 0.5166534698845417 1106 86-87 0.18207026608372345 1106 88-89 0.2738456590669145 1106 90-91 0.2619726909899178 1106 92-93 0.11636407264692394 1106 94-95 -0.010590830183438982 1106 96-97 0.24491165818605865 1106 98-99 0.061300059875978974 1106 100-101 0.09656987606414091 1107 1 0.09787520069582456 1107 2 0.026259526629452523 1107 3 0.14014273209336636 1107 4 0.09740404461454233 1107 5 0.07696189489286098 1107 6 0.10138467297990417 1107 7 0.12235227682894134 1107 8 0.05061300574848815 1107 9 0.20238094468879098 1107 10-11 0.21753301071986897 1107 12-13 0.15613722655008644 1107 14-15 0.20858197176030302 1107 16-17 0.12220679854547711 1107 18-19 0.042539469677876696 1107 20-21 0.10408517014036534 1107 22-23 0.08108719360732408 1107 24-25 0.11295336920650811 1107 26-27 0.19657834019914588 1107 28-29 0.24532859915751715 1107 30-31 0.17061552692994297 1107 32-33 0.08032908893118673 1107 34-35 0.21329234207404824 1107 36-37 0.22175107289074703 1107 38-39 0.22961968871765492 1107 40-41 0.2673308958866514 1107 42-43 0.18432502917350035 1107 44-45 0.17538446504486416 1107 46-47 0.08596757165161506 1107 48-49 0.28002408912350774 1107 50-51 0.11552584850173986 1107 52-53 0.17941702343215837 1107 54-55 0.3167668833528623 1107 56-57 0.16353111467188342 1107 58-59 0.237010463887934 1107 60-61 0.25499542188580904 1107 62-63 0.3921676779072456 1107 64-65 0.18071194293343495 1107 66-67 0.3831162618950188 1107 68-69 0.41647221438442017 1107 70-71 0.30097734305893553 1107 72-73 0.13142919789222773 1107 74-75 0.08798745602426195 1107 76-77 0.10063021766465852 1107 78-79 0.38071090270942776 1107 80-81 0.6141748754563778 1107 82-83 0.45249668324234804 1107 84-85 0.4162970664489798 1107 86-87 0.352468983635692 1107 88-89 0.3332362461817908 1107 90-91 0.36849543131086193 1107 92-93 0.2965926689039833 1107 94-95 0.4424090854175624 1107 96-97 0.5974608727022677 1107 98-99 0.05585189597103479 1107 100-101 -0.03691235377830182 1108 1 0.13739201723147687 1108 2 0.13946873367486745 1108 3 0.08591341055684865 1108 4 0.14771331303187907 1108 5 0.08085052893815003 1108 6 0.12112632440831561 1108 7 0.21313245432576622 1108 8 0.16478677836236244 1108 9 0.12274406539877702 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-1.1364805317435263 2316 100-101 -1.0052793525051875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 10.0 5 24.0 6 76.0 7 160.0 8 357.0 9 717.0 10 1273.0 11 2075.0 12 3219.0 13 4701.0 14 6020.0 15 8371.0 16 11783.0 17 16887.0 18 15353.0 19 8590.0 20 7112.0 21 6930.0 22 7333.0 23 8048.0 24 9161.0 25 10679.0 26 12807.0 27 16259.0 28 19713.0 29 24510.0 30 30668.0 31 38441.0 32 49003.0 33 64416.0 34 88234.0 35 128193.0 36 195893.0 37 282158.0 38 181538.0 39 10317.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63578420840977 19.24373312431287 14.832582953027845 21.287899714249512 2 15.787407305415135 21.320010228367988 39.66403745156278 23.228545014654102 3 20.01738740475282 26.02619125358468 29.92702797326588 24.02939336839662 4 12.95161907039758 17.294290178578454 36.86813454829832 32.885956202725644 5 13.419965870441427 38.44555517179253 33.03793195960155 15.096546998164495 6 30.80778795929944 36.50674762930169 17.38664195197787 15.298822459420993 7 27.971761684731938 34.28407782924049 20.080107691424104 17.66405279460347 8 27.10294491357071 33.91973023644436 19.274467581246522 19.70285726873841 9 27.421760797080815 16.807050615323853 20.056835436863413 35.71435315073192 10-11 24.927511600111103 27.265828043745614 26.399980171422772 21.406680184720507 12-13 26.889615503536866 24.735590332003976 27.30994092234778 21.064853242111376 14-15 23.539482266083102 25.481397502358305 26.854329410820597 24.124790820737996 16-17 21.534075665656207 29.10575521286129 27.152117283576665 22.208051837905842 18-19 21.314786251052784 27.014076318088676 30.740722820094323 20.930414610764213 20-21 22.702659537013105 25.775208207205264 30.638195258253248 20.883936997528384 22-23 22.149585211108032 26.13823044386817 30.476991490218786 21.235192854805003 24-25 22.047346631746628 26.066731266378607 30.894359799937604 20.991562301937165 26-27 21.95194298525653 26.183279771859198 30.780046256027386 21.084730986856883 28-29 21.35706620426172 26.34744413218823 31.13035350690303 21.16513615664702 30-31 21.553804357660837 26.24096874672994 30.975221931026127 21.230004964583088 32-33 21.521640524837654 26.410728384750865 30.62844639603897 21.439184694372507 34-35 21.917816841182685 26.14506714585469 30.719867761728793 21.217248251233833 36-37 22.18180144397515 25.993991829643665 30.58249982689923 21.241706899481958 38-39 22.0171329697541 26.00660328291432 30.785878794507443 21.19038495282414 40-41 21.809164743674977 26.212192855676097 30.708492293215777 21.270150107433146 42-43 21.821478333867017 26.522002865372347 30.181922033863636 21.474596766897 44-45 21.914355106334266 26.709959332483113 29.786812206391776 21.588873354790845 46-47 22.271709490068716 26.766868549543126 29.115586207764704 21.845835752623454 48-49 22.444716497481497 27.02207878243328 28.74230720207963 21.790897518005593 50-51 22.473679116943323 27.42922808074911 28.507398801997564 21.589694000310004 52-53 22.551747035299467 27.524883186117815 28.34023975660857 21.58313002197415 54-55 22.0510008269086 27.3482119183414 28.48786663089428 22.11292062385572 56-57 22.312436663246743 27.752080277957862 28.08473592114456 21.850747137650828 58-59 22.479427207186582 27.347033186720594 28.129308740786545 22.04423086530628 60-61 22.28174195736137 27.115546963942766 28.77533325937623 21.82737781931964 62-63 22.44335795506403 27.169455625808443 28.725085840354254 21.662100578773277 64-65 22.218738628876018 27.324410176725706 28.558874148087682 21.897977046310594 66-67 22.066064204260247 27.614189075425294 28.31225816400552 22.007488556308942 68-69 22.095461760419184 27.66628705402891 28.390383408764137 21.84786777678777 70-71 22.12507847990521 27.60368395296398 28.129086265741222 22.142151301389585 72-73 22.14837624966661 27.575983113028002 27.930456893926937 22.34518374337845 74-75 22.085238926578967 27.967434516171412 27.708237119396024 22.2390894378536 76-77 22.308402810613995 27.696475088490597 27.86370048440685 22.13142161648856 78-79 22.255424124801387 27.718631971465246 27.740897702341794 22.28504620139157 80-81 22.339936727633784 28.04625129719366 27.58244801587395 22.031363959298606 82-83 22.16380711079287 27.971885733170193 27.606274367861765 22.258032788175168 84-85 22.185608541793297 27.796668181308533 27.51789539529202 22.499827881606148 86-87 22.406611580651813 27.59834521653336 27.641461484112284 22.353581718702547 88-89 22.339265897766033 27.576295075943985 27.502142937785766 22.582296088504215 90-91 22.04531838167407 27.67870454619661 27.81064483554473 22.465332236584594 92-93 22.000618392698247 27.854106528763523 27.600061996621655 22.545213081916575 94-95 21.84682912705993 27.859827951642675 27.386592690383765 22.90675023091363 96-97 21.931616211763465 27.68891969570489 27.306190465325415 23.07327362720623 98-99 21.800746885453044 27.964223769808534 27.01962177368183 23.215407571056595 100-101 21.764188704109834 28.03906257683192 27.160724998278962 23.036023720779284 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 980.0 1 708.5 2 472.5 3 576.5 4 525.5 5 468.0 6 820.0 7 1709.0 8 2700.0 9 3146.5 10 2847.5 11 2449.5 12 2437.0 13 2506.0 14 2632.0 15 2727.0 16 2844.5 17 2954.0 18 3095.0 19 3242.5 20 3420.5 21 3781.5 22 4338.5 23 5012.5 24 5701.0 25 6839.5 26 8303.0 27 10126.5 28 11783.5 29 13270.0 30 15275.0 31 17468.0 32 19226.5 33 21107.5 34 23790.0 35 25647.0 36 27886.0 37 30875.0 38 32337.5 39 32829.5 40 34188.5 41 36334.5 42 38373.0 43 40274.0 44 43067.5 45 45272.5 46 45117.5 47 46873.5 48 56339.5 49 56686.5 50 48164.5 51 44971.0 52 42768.5 53 42051.5 54 41005.0 55 37945.0 56 33827.5 57 30444.5 58 28496.0 59 25835.5 60 21760.5 61 17867.0 62 14451.0 63 11130.5 64 8022.5 65 5805.5 66 4222.5 67 3216.5 68 2338.0 69 1593.0 70 1165.0 71 866.5 72 644.5 73 485.0 74 385.0 75 295.5 76 183.0 77 79.5 78 42.0 79 25.0 80 15.0 81 12.0 82 10.0 83 7.0 84 6.5 85 2.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027536527203335225 2 0.0050352506886098694 3 3.1470316803811684E-4 4 7.867579200952921E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 7.867579200952921E-5 10-11 0.011486665633391265 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.7536527203335224E-4 20-21 7.474200240905276E-4 22-23 0.0024389495522954057 24-25 0.010660569817291209 26-27 0.00546796754466228 28-29 2.3602737602858764E-4 30-31 0.002832328512343052 32-33 0.004366506456528871 34-35 0.0 36-37 0.008103606576981509 38-39 0.012273423553486556 40-41 0.0012588126721524674 42-43 0.0 44-45 0.0 46-47 3.933789600476461E-4 48-49 0.0035404106404288145 50-51 0.007080821280857629 52-53 0.0 54-55 0.0031470316803811687 56-57 0.0043271685605241065 58-59 2.7536527203335224E-4 60-61 0.0025176253443049347 62-63 0.005900684400714691 64-65 1.1801368801429382E-4 66-67 0.002596301136314464 68-69 5.113926480619399E-4 70-71 0.0010227852961238798 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0021635842802620532 80-81 0.0020455705922477595 82-83 0.012116071969467498 84-85 0.008772350809062508 86-87 0.004484520144543165 88-89 2.7536527203335224E-4 90-91 7.474200240905276E-4 92-93 1.5735158401905842E-4 94-95 7.867579200952921E-5 96-97 0.002832328512343052 98-99 0.0021635842802620532 100-101 1.1801368801429382E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1271039.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.44821292528811 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09425623000233 46.81366142223761 2 11.791264036732715 13.783566221544922 3 3.4193260286515272 5.995604873508128 4 1.4678349041789591 3.4316930807448673 5 0.8242017273670811 2.408655902727071 6 0.4801609136177697 1.6838728390519364 7 0.34868003236920847 1.4265807342298293 8 0.24241393741274397 1.133492914396602 9 0.1733901428502604 0.9120909589612327 >10 1.0352677129677978 11.192905599069181 >50 0.07912273226898844 3.172070620094859 >100 0.03990262506025294 4.222603947969935 >500 0.0025613081899496484 0.9209940677655314 >1k 0.001213251247870886 1.1800605828492055 >5k 4.0441708262362866E-4 1.7221462348491077 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9347 0.7353826279130695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7478 0.5883375726472595 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.395896585391951 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2799 0.2202135418346723 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2441 0.1920476082952608 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 0.16521916322001134 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1722 0.1354797138404093 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.11392254682979831 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1285 0.10109839273224504 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 7.867579200952921E-5 0.0 0.0 0.0 5 0.0 7.867579200952921E-5 0.0 0.0 0.0 6 7.867579200952921E-5 7.867579200952921E-5 0.0 0.0 0.0 7 7.867579200952921E-5 7.867579200952921E-5 0.0 0.0 0.0 8 7.867579200952921E-5 7.867579200952921E-5 0.0 0.0 0.0 9 7.867579200952921E-5 7.867579200952921E-5 0.0 0.0 0.0 10-11 7.867579200952921E-5 7.867579200952921E-5 0.0 0.0 0.0 12-13 7.867579200952921E-5 7.867579200952921E-5 0.0 7.867579200952921E-5 0.0 14-15 7.867579200952921E-5 7.867579200952921E-5 0.0 7.867579200952921E-5 0.0 16-17 7.867579200952921E-5 7.867579200952921E-5 0.0 1.1801368801429382E-4 0.0 18-19 1.9668948002382305E-4 7.867579200952921E-5 0.0 1.5735158401905842E-4 0.0 20-21 2.3602737602858764E-4 7.867579200952921E-5 0.0 2.3602737602858764E-4 0.0 22-23 2.3602737602858764E-4 7.867579200952921E-5 0.0 5.507305440667045E-4 0.0 24-25 2.3602737602858764E-4 7.867579200952921E-5 0.0 0.0011407989841381735 0.0 26-27 2.3602737602858764E-4 7.867579200952921E-5 0.0 0.00196689480023823 0.0 28-29 2.7536527203335224E-4 7.867579200952921E-5 0.0 0.004091141184495519 0.0 30-31 3.1470316803811684E-4 7.867579200952921E-5 0.0 0.00837897184901486 0.0 32-33 3.1470316803811684E-4 7.867579200952921E-5 0.0 0.013571574121643788 0.0 34-35 3.5404106404288144E-4 7.867579200952921E-5 0.0 0.01719066055408213 0.0 36-37 3.9337896004764604E-4 7.867579200952921E-5 0.0 0.02238326282671106 0.0 38-39 3.9337896004764604E-4 7.867579200952921E-5 0.0 0.028913353563501984 0.0 40-41 3.9337896004764604E-4 7.867579200952921E-5 0.0 0.0426816171651696 0.0 42-43 3.9337896004764604E-4 7.867579200952921E-5 0.0 0.05613517759879909 0.0 44-45 4.720547520571753E-4 7.867579200952921E-5 0.0 0.07021814436850482 0.0 46-47 7.474200240905275E-4 7.867579200952921E-5 0.0 0.09799069894786863 0.0 48-49 7.867579200952921E-4 7.867579200952921E-5 0.0 0.11266373415764583 0.0 50-51 7.867579200952921E-4 7.867579200952921E-5 0.0 0.13319811587213296 0.0 52-53 7.867579200952921E-4 7.867579200952921E-5 0.0 0.15510932394678684 0.0 54-55 7.867579200952921E-4 7.867579200952921E-5 0.0 0.18693368181464143 0.0 56-57 9.441095041143506E-4 7.867579200952921E-5 0.0 0.2569551367031224 0.0 58-59 9.441095041143506E-4 7.867579200952921E-5 0.0 0.3213906103589268 0.0 60-61 9.441095041143506E-4 7.867579200952921E-5 0.0 0.37123172459696363 0.0 62-63 9.441095041143506E-4 7.867579200952921E-5 0.0 0.4263441168996388 0.0 64-65 9.441095041143506E-4 7.867579200952921E-5 0.0 0.4813778334103045 0.0 66-67 9.441095041143506E-4 7.867579200952921E-5 0.0 0.5715009531572202 0.0 68-69 9.441095041143506E-4 7.867579200952921E-5 0.0 0.6816077240745564 0.0 70-71 9.441095041143506E-4 7.867579200952921E-5 0.0 0.7369168058572553 0.0 72-73 9.83447400119115E-4 7.867579200952921E-5 0.0 0.8018636721611216 0.0 74-75 0.0010227852961238798 7.867579200952921E-5 0.0 0.9218835928716586 0.0 76-77 0.0010227852961238798 7.867579200952921E-5 0.0 1.0598809320563727 0.0 78-79 0.0010227852961238798 7.867579200952921E-5 0.0 1.1932757374085297 0.0 80-81 0.001101461088133409 7.867579200952921E-5 0.0 1.3004321661255083 0.0 82-83 0.001101461088133409 7.867579200952921E-5 0.0 1.47143400005822 0.0 84-85 0.001101461088133409 7.867579200952921E-5 0.0 1.765248745317807 0.0 86-87 0.001101461088133409 7.867579200952921E-5 0.0 2.020433676700715 0.0 88-89 0.001101461088133409 7.867579200952921E-5 0.0 2.3336420046906508 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAAC 65 0.005848893 29.226072 2 GTATCAA 13470 0.0 26.764946 1 GGTATCA 9595 0.0 25.54447 1 GAGTACT 7685 0.0 23.638037 12-13 TATCAAC 15715 0.0 22.847109 2 ATCAACG 15680 0.0 22.837528 3 TCAACGC 15770 0.0 22.737309 4 CAACGCA 15915 0.0 22.507393 5 GTACATG 7790 0.0 22.499924 1 TACCTGG 890 0.0 22.41213 2 TACATGG 7850 0.0 22.203438 2 AACGCAG 16145 0.0 22.186756 6 GTACTTT 8125 0.0 22.065683 14-15 AGTACTT 7845 0.0 21.612207 12-13 TACAACG 110 0.0028802154 21.58744 4 ACATGGG 7795 0.0 20.958786 3 GTACCTG 1065 0.0 20.516365 1 ACGCAGA 17600 0.0 20.325577 7 CGCAGAG 17675 0.0 20.104935 8 ACTTTTT 9110 0.0 19.993458 16-17 >>END_MODULE