FastQCFastQC Report
Thu 26 May 2016
SRR937012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307206
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98670.7548159968665994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.55492401350667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.3527370590404267No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23520.1799257347349997No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19970.15276857664362006No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18660.14274720281271658No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17950.13731577119444066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.10373269400538249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG600.00393987231.6851581
GGTATCA81300.029.6390691
GTATCAA116200.028.4675621
GTACATG83550.026.3379341
TACATGG84300.025.6555232
TGTACGC950.001221658425.0174732
ACATGGG85250.024.8678113
GAGTACT70000.024.336812-13
TATCAAC136500.024.3063562
ATCAACG136650.024.2796733
TCAACGC138750.023.9464574
CAACGCA139750.023.7741935
ATTCGCG1002.0547304E-823.75204880-81
AACGCAG142550.023.3369856
TACCTGG10000.022.8159372
GTACTTT76600.022.08311514-15
ACGCAGA155050.021.4232837
AGTACTT72350.021.31319212-13
CGCAGAG155700.021.2422898
TACTACG1353.7784572E-421.1258662
Produced by FastQC (version 0.11.5)