##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937012_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1307206 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.718864509495827 33.0 31.0 34.0 30.0 34.0 2 31.781526400582617 33.0 31.0 34.0 30.0 34.0 3 31.79375859657927 33.0 31.0 34.0 30.0 34.0 4 35.405614723310634 37.0 35.0 37.0 33.0 37.0 5 35.413208782701425 37.0 35.0 37.0 33.0 37.0 6 35.48895353907494 37.0 35.0 37.0 33.0 37.0 7 35.43951756647384 37.0 35.0 37.0 33.0 37.0 8 35.39889734288246 37.0 35.0 37.0 33.0 37.0 9 37.00054773310404 39.0 37.0 39.0 33.0 39.0 10-11 36.876426133294984 39.0 37.0 39.0 33.0 39.0 12-13 36.78353641277656 39.0 37.0 39.0 32.5 39.0 14-15 37.87959816585909 40.0 38.0 41.0 33.0 41.0 16-17 37.82197450134103 40.0 37.5 41.0 33.0 41.0 18-19 37.82184904292055 40.0 38.0 41.0 33.0 41.0 20-21 37.778663041632306 40.0 37.5 41.0 32.0 41.0 22-23 37.672202009476706 40.0 37.0 41.0 32.0 41.0 24-25 37.612961920309424 40.0 37.0 41.0 32.0 41.0 26-27 37.60539540057191 40.0 37.0 41.0 32.0 41.0 28-29 37.51381304859372 40.0 37.0 41.0 32.0 41.0 30-31 37.322043732969405 40.0 37.0 41.0 32.0 41.0 32-33 37.08896225996515 39.5 36.0 41.0 31.0 41.0 34-35 36.9013923589702 39.0 36.0 41.0 31.0 41.0 36-37 36.69343163969566 39.0 35.0 41.0 30.0 41.0 38-39 36.51313794459328 39.0 35.0 41.0 30.0 41.0 40-41 36.29985365734245 39.0 35.0 41.0 30.0 41.0 42-43 36.04202130345179 39.0 35.0 40.5 29.5 41.0 44-45 35.724498663561825 39.0 35.0 40.0 28.5 41.0 46-47 35.290035388454456 38.0 35.0 40.0 26.5 41.0 48-49 34.9647178792019 38.5 34.5 40.0 24.5 41.0 50-51 34.23174235736372 38.0 34.0 39.5 23.5 40.5 52-53 34.40014351219318 38.0 34.0 39.5 24.0 40.5 54-55 34.77952021334052 39.0 35.0 40.0 24.0 41.0 56-57 34.66244455732303 38.5 34.5 40.0 23.5 41.0 58-59 34.436041067742956 38.0 34.0 40.0 23.0 41.0 60-61 34.14380747946383 38.0 34.0 40.0 22.5 41.0 62-63 33.826875029643375 37.5 34.0 40.0 22.0 41.0 64-65 33.478601306909546 37.0 33.0 40.0 21.5 41.0 66-67 33.10671806891951 36.0 33.0 39.0 20.0 41.0 68-69 32.721185872769865 36.0 32.5 39.0 18.5 41.0 70-71 32.30015850600441 35.0 32.0 39.0 17.5 40.0 72-73 31.90570499217415 35.0 32.0 38.0 13.5 40.0 74-75 31.51365163562591 35.0 31.5 37.0 10.0 39.5 76-77 31.06823905337032 35.0 31.0 37.0 7.5 39.0 78-79 30.61801506419034 35.0 31.0 36.0 6.5 39.0 80-81 30.194048986923256 34.5 30.5 36.0 2.0 37.5 82-83 29.80938964478437 34.0 30.0 35.0 2.0 37.0 84-85 29.454726722490562 34.0 30.0 35.0 2.0 36.5 86-87 29.099318317082385 34.0 29.0 35.0 2.0 36.0 88-89 28.738357994072853 34.0 29.0 35.0 2.0 36.0 90-91 28.378657610200687 34.0 29.0 35.0 2.0 35.5 92-93 28.013469950413324 34.0 28.5 35.0 2.0 35.0 94-95 27.650143129697994 34.0 27.0 35.0 2.0 35.0 96-97 27.166091266410955 34.0 26.5 35.0 2.0 35.0 98-99 26.610989392643546 34.0 25.0 35.0 2.0 35.0 100-101 24.51982510790189 31.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18599196686075814 1101 2 0.1014106879426322 1101 3 0.16320985150776934 1101 4 0.12562783302400504 1101 5 0.12750686131373357 1101 6 7.547729442549667E-4 1101 7 0.052705005242884795 1101 8 0.1580711946414084 1101 9 0.07772152754839823 1101 10-11 0.19750328059508604 1101 12-13 0.22279352971319355 1101 14-15 0.2815259438925679 1101 16-17 0.33313176775941855 1101 18-19 0.30906712910888956 1101 20-21 0.17941188999527213 1101 22-23 0.25480290188529153 1101 24-25 0.262328402256081 1101 26-27 0.23837708935453605 1101 28-29 0.2946275217044416 1101 30-31 0.1457860161404767 1101 32-33 0.18910580428734391 1101 34-35 0.19611199243855992 1101 36-37 0.29022778686420025 1101 38-39 0.214416992759908 1101 40-41 0.1937603330596147 1101 42-43 0.26146691382565024 1101 44-45 0.3288816583381262 1101 46-47 0.3780970559989356 1101 48-49 0.4626737644003214 1101 50-51 0.4973371668891602 1101 52-53 0.6540887241684175 1101 54-55 0.5906118599107728 1101 56-57 0.5873235779968482 1101 58-59 0.7840553870037255 1101 60-61 0.7608136713207188 1101 62-63 0.8249521712696222 1101 64-65 0.5363470692726366 1101 66-67 0.551338345633809 1101 68-69 0.35975342753221184 1101 70-71 0.4720465228568287 1101 72-73 0.5402796610367453 1101 74-75 0.38780092727272475 1101 76-77 0.31852123669682797 1101 78-79 0.4087615289139883 1101 80-81 0.3973096445692832 1101 82-83 0.3177180211132935 1101 84-85 0.37058695335210246 1101 86-87 0.40049915671824365 1101 88-89 0.6089594141585835 1101 90-91 0.42784814079767486 1101 92-93 0.42326331440876785 1101 94-95 0.4802397514531691 1101 96-97 0.19096233735637114 1101 98-99 0.08499944369162904 1101 100-101 0.3462754231518126 1102 1 0.1425882314793654 1102 2 -0.02652787026996961 1102 3 -0.015070825748296102 1102 4 0.09240213918563711 1102 5 0.17521326731827003 1102 6 0.06288428943928182 1102 7 0.012022512661282292 1102 8 -0.019605537772626747 1102 9 0.23929771885578077 1102 10-11 0.030337981240926126 1102 12-13 0.016442508592582783 1102 14-15 -0.13666134882867453 1102 16-17 0.17806543855711965 1102 18-19 -0.07192519066768455 1102 20-21 -0.1180457815006335 1102 22-23 -0.0485676688949539 1102 24-25 -0.10509872289665623 1102 26-27 -0.11297305901342014 1102 28-29 -0.20236409987000314 1102 30-31 -0.17120909644461335 1102 32-33 -0.10800625715444312 1102 34-35 -0.26225945109434434 1102 36-37 -0.03190958440477942 1102 38-39 0.037904512396849555 1102 40-41 -0.14564881338827718 1102 42-43 0.032214862856896787 1102 44-45 0.07158806434265585 1102 46-47 -0.14879856278270154 1102 48-49 0.14427962929821092 1102 50-51 0.034653452453831335 1102 52-53 0.09469965103174616 1102 54-55 -0.1063003411714405 1102 56-57 -0.04375950805656004 1102 58-59 -0.2911436006031849 1102 60-61 -0.18575117420206055 1102 62-63 -0.3565498360614967 1102 64-65 -0.2648826392279702 1102 66-67 -0.5025803138181004 1102 68-69 -0.1918393460757457 1102 70-71 -0.3167167866212637 1102 72-73 -0.29679312653525614 1102 74-75 -0.14183164385661584 1102 76-77 -0.271308576534107 1102 78-79 -0.3194982022781936 1102 80-81 -0.26270519222596533 1102 82-83 -0.13897599180344145 1102 84-85 -0.18892255965191396 1102 86-87 -0.24292290646904746 1102 88-89 0.05161607326139617 1102 90-91 0.025044859247667972 1102 92-93 0.04782955633580954 1102 94-95 0.28142233131003636 1102 96-97 0.17138562239738775 1102 98-99 0.29749289804666645 1102 100-101 0.2518846045098151 1103 1 0.27986005955238014 1103 2 0.2677208483704412 1103 3 0.23249683903005192 1103 4 0.20404138026108143 1103 5 0.2454569504402926 1103 6 0.21426635939701555 1103 7 0.17422015675803948 1103 8 0.2583029236966681 1103 9 0.027935431291716384 1103 10-11 0.14907190804606074 1103 12-13 0.1447097507470616 1103 14-15 0.37456515957884307 1103 16-17 0.3640675966364242 1103 18-19 0.31265892946539253 1103 20-21 0.36897516985266776 1103 22-23 0.3497939892293189 1103 24-25 0.3754300065341525 1103 26-27 0.45095284693029924 1103 28-29 0.34547422402173567 1103 30-31 0.5285400268356639 1103 32-33 0.42226979715721313 1103 34-35 0.5152920280891777 1103 36-37 0.3327500685041329 1103 38-39 0.6438644080896765 1103 40-41 0.6282968749491005 1103 42-43 0.6689624574976634 1103 44-45 0.606983262616204 1103 46-47 0.6254321718634657 1103 48-49 0.7777361529921265 1103 50-51 0.7221410884577892 1103 52-53 0.7160673337940864 1103 54-55 0.7743551754187976 1103 56-57 0.8571720628453363 1103 58-59 0.8280749948468653 1103 60-61 0.807052530500755 1103 62-63 0.7564049341929717 1103 64-65 0.7417570514473297 1103 66-67 0.7433526058833593 1103 68-69 0.571705299189972 1103 70-71 0.6058522269566495 1103 72-73 0.5526682528371047 1103 74-75 0.5113749023172893 1103 76-77 0.6442520744864844 1103 78-79 0.6526652722294948 1103 80-81 0.5507944930541342 1103 82-83 0.725436381184597 1103 84-85 0.7948828535303569 1103 86-87 0.8688503153635203 1103 88-89 0.7441644052459289 1103 90-91 0.8306288894607796 1103 92-93 0.807291834907879 1103 94-95 0.9158814626831138 1103 96-97 0.7761405904757979 1103 98-99 0.6982347378092761 1103 100-101 0.9692076869664312 1104 1 0.11368522217335197 1104 2 0.036918849322368885 1104 3 0.04338847793216161 1104 4 0.21819019828424757 1104 5 0.2791329777288851 1104 6 -0.026933862719431545 1104 7 0.220872544509902 1104 8 0.17594769602730054 1104 9 0.3604009239142769 1104 10-11 0.40905009303733664 1104 12-13 0.2031315340248696 1104 14-15 0.04463496760676122 1104 16-17 0.31998501691248293 1104 18-19 0.3936790786007265 1104 20-21 0.21561684841842776 1104 22-23 0.17169387817109083 1104 24-25 0.2723314820220182 1104 26-27 0.24726452390951437 1104 28-29 0.35773346565270003 1104 30-31 0.4015005218380452 1104 32-33 0.06089822806313805 1104 34-35 0.11881217722451254 1104 36-37 0.17247678594027605 1104 38-39 0.23407898844823194 1104 40-41 0.24554659730353023 1104 42-43 0.31221606689000936 1104 44-45 0.2729554187323302 1104 46-47 0.11518744712921603 1104 48-49 0.09528771574002093 1104 50-51 0.03547005878937881 1104 52-53 0.1535590044271089 1104 54-55 0.29148035390522864 1104 56-57 0.1806481853266959 1104 58-59 0.03913084126919841 1104 60-61 0.010009852410952647 1104 62-63 -0.03898376959887173 1104 64-65 -0.1088053791412733 1104 66-67 -0.29640187610934277 1104 68-69 -0.06383372984382163 1104 70-71 -0.0056097752645101195 1104 72-73 -0.06970878984098405 1104 74-75 -0.10622663663241738 1104 76-77 0.035908825240095865 1104 78-79 0.18423119321949954 1104 80-81 0.20182535137556812 1104 82-83 0.10052522776558703 1104 84-85 0.057735860035194264 1104 86-87 0.17331406278538353 1104 88-89 -0.18444141737821695 1104 90-91 -0.1452516436187068 1104 92-93 -0.006327076721504454 1104 94-95 0.12348736463456689 1104 96-97 0.013443858760744831 1104 98-99 -0.09234117842109058 1104 100-101 -0.1376620575996519 1105 1 0.17156235861664015 1105 2 0.36997157651073564 1105 3 0.29900238529019774 1105 4 0.21830797256448164 1105 5 0.20502440411817702 1105 6 0.16359770970996124 1105 7 0.18235054034854414 1105 8 0.2523327929412602 1105 9 0.16700217232164505 1105 10-11 0.18016938589200748 1105 12-13 0.11856982387093495 1105 14-15 0.42776897689308413 1105 16-17 0.37085882177121476 1105 18-19 0.10147841264070223 1105 20-21 0.26417130296290736 1105 22-23 0.14038331810085225 1105 24-25 0.08960736292009841 1105 26-27 0.29341798915003636 1105 28-29 0.40331211210502005 1105 30-31 0.39634878930700523 1105 32-33 0.3583545712788023 1105 34-35 0.1807781779311597 1105 36-37 0.14318199513238028 1105 38-39 0.43085951250809273 1105 40-41 0.3542589496841799 1105 42-43 0.5413805029831948 1105 44-45 0.5643167198883035 1105 46-47 0.5864199638942011 1105 48-49 0.5407410485737074 1105 50-51 0.5221751716207095 1105 52-53 0.5851749746195125 1105 54-55 0.37728694712357225 1105 56-57 0.4583358479355013 1105 58-59 0.3580613615816901 1105 60-61 0.5377465876365264 1105 62-63 0.47741317612145906 1105 64-65 0.43024437870766974 1105 66-67 0.2546905249513429 1105 68-69 0.3025127603041824 1105 70-71 0.2386259767242649 1105 72-73 0.46763881737821933 1105 74-75 0.6187077398955232 1105 76-77 0.4909986816625427 1105 78-79 0.5331009170209668 1105 80-81 0.2850567475004375 1105 82-83 0.3644640815006426 1105 84-85 0.46578202933750745 1105 86-87 0.6702006283089297 1105 88-89 0.7674332904166548 1105 90-91 0.5264583497074149 1105 92-93 0.6199608564112573 1105 94-95 0.8452152917715452 1105 96-97 0.5386825747355779 1105 98-99 0.5978598230171848 1105 100-101 0.6009762932749148 1106 1 0.3877361478293544 1106 2 0.3941850449014481 1106 3 0.360673932908707 1106 4 0.31054202141650933 1106 5 0.23574783253518206 1106 6 0.23861406810998176 1106 7 0.44601606686129713 1106 8 0.45666021599605244 1106 9 0.560291508770284 1106 10-11 0.5625240238589839 1106 12-13 0.5197438332198985 1106 14-15 0.4815065107295453 1106 16-17 0.7084483754715478 1106 18-19 0.6770633298163418 1106 20-21 0.5881814869208952 1106 22-23 0.4375877396511285 1106 24-25 0.40241792581606006 1106 26-27 0.36573046723129465 1106 28-29 0.3150382383296204 1106 30-31 0.4341909683217935 1106 32-33 0.39008379459259857 1106 34-35 0.502465544995438 1106 36-37 0.3539783873271105 1106 38-39 0.3329269713615943 1106 40-41 0.4493031515233028 1106 42-43 0.40609615527723975 1106 44-45 0.28178855383574586 1106 46-47 0.5488890119795542 1106 48-49 0.6063796030832336 1106 50-51 0.6520965450279448 1106 52-53 0.5318458314656738 1106 54-55 0.7054417651468086 1106 56-57 0.6387526797043463 1106 58-59 0.6688990482989752 1106 60-61 0.6431941246156256 1106 62-63 0.5584438084590246 1106 64-65 0.2733707384284969 1106 66-67 0.4028558791505503 1106 68-69 0.36202317730294453 1106 70-71 0.5422340856324936 1106 72-73 0.572347000221864 1106 74-75 0.48787014426910247 1106 76-77 0.5201682518503254 1106 78-79 0.4588466854710411 1106 80-81 0.638827833136471 1106 82-83 0.5362341135190789 1106 84-85 0.7391563231935798 1106 86-87 0.8513697187515241 1106 88-89 0.8439207661826913 1106 90-91 0.8661354022501406 1106 92-93 0.9998841275447141 1106 94-95 1.1215614248886894 1106 96-97 1.3429381878301925 1106 98-99 1.210669778640753 1106 100-101 1.7031855502315985 1107 1 0.27977802905633453 1107 2 0.298653100831352 1107 3 0.33635709766737776 1107 4 0.10861771687563504 1107 5 0.19327493924554062 1107 6 0.2602864327419354 1107 7 0.3233419479180455 1107 8 0.23083814855673523 1107 9 0.410184997012621 1107 10-11 0.3270124600630524 1107 12-13 0.4992556878248493 1107 14-15 0.5355789600034697 1107 16-17 0.6113258479392627 1107 18-19 0.5992160612932267 1107 20-21 0.6763873489686674 1107 22-23 0.7153836437361676 1107 24-25 0.508988199933107 1107 26-27 0.6290299555965717 1107 28-29 0.5515111859981872 1107 30-31 0.5773558924986588 1107 32-33 0.5602354408553438 1107 34-35 0.48278865036287755 1107 36-37 0.3511166965682193 1107 38-39 0.6270471913361604 1107 40-41 0.6202964889232376 1107 42-43 0.6303560108657322 1107 44-45 0.6392516470979572 1107 46-47 0.6983630732338639 1107 48-49 0.44036016380083964 1107 50-51 0.6107026595830547 1107 52-53 0.7129347339098899 1107 54-55 0.6703945500568977 1107 56-57 0.6917821767229668 1107 58-59 0.6883350797725498 1107 60-61 0.8727816368508812 1107 62-63 0.8795746890665512 1107 64-65 0.9278813517754543 1107 66-67 1.0469218975259054 1107 68-69 0.794035930342261 1107 70-71 0.8305984149512469 1107 72-73 0.8756758768479109 1107 74-75 0.9977100692734773 1107 76-77 1.0257252456889567 1107 78-79 0.8981864071455341 1107 80-81 0.9690453133590928 1107 82-83 0.9906713744291409 1107 84-85 0.9486948589347186 1107 86-87 0.9755637876661645 1107 88-89 0.8900450267475719 1107 90-91 1.0757649635777433 1107 92-93 1.1222807366642975 1107 94-95 1.0414518158967816 1107 96-97 0.6962105576636048 1107 98-99 0.7690946104207406 1107 100-101 1.0013419274605404 1108 1 0.01842456046300711 1108 2 0.017563518372416098 1108 3 -0.020596001028756916 1108 4 0.1373435547942492 1108 5 0.008796208334224787 1108 6 -0.054830693682653475 1108 7 -0.12168311930454223 1108 8 0.01899763328348314 1108 9 -0.06388472537999945 1108 10-11 -0.06321870262252816 1108 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2316 78-79 -3.3124651012729736 2316 80-81 -3.1633492701593973 2316 82-83 -3.6621041403094132 2316 84-85 -3.390635116871337 2316 86-87 -3.6957867529944757 2316 88-89 -3.5874564545144665 2316 90-91 -4.122753774387004 2316 92-93 -4.056887164387401 2316 94-95 -4.3262467966544484 2316 96-97 -4.89519133341426 2316 98-99 -5.020757510252739 2316 100-101 -4.871807112187906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2780.0 3 1040.0 4 1069.0 5 1138.0 6 1238.0 7 1486.0 8 1850.0 9 2344.0 10 2981.0 11 3829.0 12 4986.0 13 6452.0 14 7411.0 15 9361.0 16 12056.0 17 16588.0 18 14153.0 19 8396.0 20 7865.0 21 8130.0 22 8717.0 23 9558.0 24 10712.0 25 12150.0 26 14514.0 27 16526.0 28 19950.0 29 23915.0 30 29381.0 31 36408.0 32 46277.0 33 60285.0 34 83167.0 35 119556.0 36 177136.0 37 257856.0 38 226927.0 39 39003.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.66649066017738 18.30337396787499 14.465820305026899 21.564315066920727 2 15.418383212639492 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22.55825701590898 25.458191573637862 30.80252890366974 21.181022506783417 22-23 22.137455693025363 25.616548004579155 30.749958677432982 21.4960376249625 24-25 21.87210114089531 25.63420582019184 31.07772050455689 21.415972534355962 26-27 21.779386682446308 25.73793987696316 30.998466533988132 21.484206906602395 28-29 21.310987540178935 25.921576532862478 31.355310122280137 21.412125804678446 30-31 21.438399315146306 25.846877210215734 31.180045297349444 21.534678177288516 32-33 21.33616295629296 25.973291735631715 30.894747129861866 21.795798178213456 34-35 21.85725460794458 25.78498107476948 30.76307217981551 21.594692137470425 36-37 22.010696079035156 25.52855928076691 30.81638984189985 21.64435479829808 38-39 21.91557864063335 25.650816990151444 30.906352812292127 21.527251556923083 40-41 21.73711969594036 25.797980586208375 30.881209873369684 21.583689844481572 42-43 21.542300184791127 26.04143437084378 30.525382864954825 21.890882579410267 44-45 21.722009231347105 26.24140657363409 30.130820130257852 21.90576406476096 46-47 22.000222615858743 26.278731120205297 29.528081154573055 22.19296510936291 48-49 22.190147716549767 26.424275713079755 29.18739641500781 22.198180155362667 50-51 22.117394244657152 26.81713444637568 29.06409122622341 22.00138008274376 52-53 22.22458110944459 26.868056982044784 28.878249669978928 22.029112238531695 54-55 21.790154768489934 26.73775045924063 29.01712177584773 22.45497299642171 56-57 22.11440842972437 27.265702641518676 28.43532460299184 22.184564325765116 58-59 22.29626036810304 26.68999505789001 28.654063011137342 22.359681562869607 60-61 22.109353155312416 26.46418575723023 29.300889671071477 22.125571416385874 62-63 22.260381920004896 26.52863634972611 29.19564984545705 22.015331884811946 64-65 22.025962262649784 26.717522213122813 29.08837550533922 22.16814001888818 66-67 21.902068558665782 26.995821105197738 28.802964517078074 22.29914581905841 68-69 21.831307671290958 27.079954785172166 28.935576523494035 22.15316102004284 70-71 21.87907339867355 27.000524303575595 28.597889773784058 22.522512523966796 72-73 21.85456327250041 27.024819847922153 28.505932137585354 22.614684741992082 74-75 21.838307893409006 27.356108317040075 28.379111935539527 22.426471854011393 76-77 22.127305162745838 26.99852823084318 28.439500034623112 22.434666571787865 78-79 22.030844689984466 27.0327576576094 28.447665031717 22.488732620689127 80-81 21.992511421957104 27.36557765093519 28.344520522776545 22.297390404331157 82-83 21.959772135713084 27.147399758423145 28.39151672692059 22.501311378943175 84-85 21.943630269408416 27.036629843257597 28.24228471924159 22.7774551680924 86-87 22.13237283122764 26.905384191277047 28.473928818621435 22.488314158873873 88-89 22.190277931908025 26.789643468045295 28.263757878181405 22.75632072186528 90-91 21.823256532990882 26.819290303151593 28.68114152418788 22.676311639669645 92-93 21.880658536996023 27.086971761460955 28.455658498730347 22.576711202812678 94-95 21.755681387817123 27.121736209776202 28.224387944891898 22.89819445751478 96-97 21.741103126606763 27.0074584384105 28.090607631662706 23.16083080332003 98-99 21.66471009590513 27.141985814734433 28.020682509022677 23.172621580337765 100-101 21.740794669336896 26.951190197312314 28.36155908422099 22.9464560491298 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 603.0 1 467.0 2 438.5 3 552.5 4 460.0 5 473.5 6 886.0 7 1866.0 8 3157.5 9 3655.0 10 3068.5 11 2413.5 12 2247.0 13 2402.5 14 2585.0 15 2662.0 16 2693.5 17 2767.5 18 2915.5 19 3100.5 20 3364.5 21 3718.0 22 4255.0 23 4944.5 24 5730.0 25 6866.0 26 8485.5 27 10333.0 28 11732.5 29 13327.0 30 15425.5 31 17720.5 32 20064.0 33 21906.0 34 24136.5 35 26503.0 36 29123.5 37 31969.5 38 33265.5 39 33795.0 40 35224.0 41 37536.0 42 39784.0 43 41535.0 44 44137.5 45 46459.0 46 46954.0 47 48876.5 48 58004.5 49 58282.0 50 49415.5 51 45992.5 52 44030.0 53 43554.0 54 42303.5 55 38952.0 56 35223.5 57 31700.5 58 29274.0 59 26598.0 60 22548.0 61 18435.5 62 14841.5 63 11552.5 64 8303.5 65 5965.5 66 4308.5 67 3279.0 68 2444.0 69 1665.5 70 1191.0 71 837.5 72 616.0 73 497.0 74 373.0 75 265.0 76 176.0 77 101.0 78 57.0 79 33.0 80 24.0 81 18.5 82 15.5 83 8.0 84 3.0 85 4.0 86 7.5 87 6.0 88 3.0 89 3.5 90 3.0 91 1.5 92 0.5 93 0.0 94 0.0 95 1.5 96 1.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033353580078426814 2 0.024326693726925978 3 0.0237912004687861 4 0.04299245872494466 5 0.08674990781866056 6 0.107098651627976 7 0.05492630847777626 8 0.07848801183593099 9 0.06716615437811638 10-11 0.06915512933692165 12-13 0.06735740197030919 14-15 0.0609697323910692 16-17 0.04876813600916764 18-19 0.0566475368075116 20-21 0.05439081521963639 22-23 0.03167060126713005 24-25 0.009065135869939397 26-27 0.0027922148460150886 28-29 0.006464168616117124 30-31 0.005010686915451735 32-33 0.006311170542362871 34-35 0.006770164763625627 36-37 0.0069996618742570035 38-39 0.0046281917310661055 40-41 0.007190909466449817 42-43 0.004169197509803352 44-45 0.0022567215878752087 46-47 0.001644729292858203 48-49 4.972437397013171E-4 50-51 0.002906963401330777 52-53 0.007076160911134129 54-55 0.012010348789708738 56-57 0.00895038731462371 58-59 0.005507930655153052 60-61 0.0028687138828922144 62-63 0.009638878646517841 64-65 0.003021711956646466 66-67 0.02153447888091089 68-69 0.042456965466804775 70-71 0.05439081521963639 72-73 0.0035572052147863457 74-75 0.013196083861304187 76-77 0.021075484659648133 78-79 0.02570367639071424 80-81 0.07267408503326944 82-83 0.028725388347360704 84-85 0.012010348789708738 86-87 0.029796374863640465 88-89 0.0452109307943813 90-91 0.04203622076398058 92-93 0.042227468356173394 94-95 0.013387331453497 96-97 0.017594778481738915 98-99 0.03809652036480861 100-101 0.07282708310702368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1307206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.15266976636467 #Duplication Level Percentage of deduplicated Percentage of total 1 81.03314195015864 48.743598278588436 2 11.344458975682658 13.64798988884621 3 3.1992466082142594 5.7732967417522385 4 1.3948147985201307 3.356073358424796 5 0.7716881527653575 2.3209551307955256 6 0.4508968134720908 1.6273588271695685 7 0.3291529639234473 1.3859600679055086 8 0.2277906449423726 1.0961772352868613 9 0.16903192493999852 0.9150949404799805 >10 0.9683327493663567 10.764886409187843 >50 0.07015605186303814 2.868378294870134 >100 0.03785827609364338 3.9949291049194366 >500 0.0021596863328603856 0.955456451435213 >1k 0.0010163229801695933 1.241489683277331 >5k 2.5408074504239833E-4 1.3083555870608878 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9867 0.7548159968665994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7254 0.55492401350667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4611 0.3527370590404267 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2352 0.1799257347349997 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1997 0.15276857664362006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1866 0.14274720281271658 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1795 0.13731577119444066 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1356 0.10373269400538249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.649903687712572E-5 0.0 12-13 0.0 0.0 0.0 7.649903687712572E-5 0.0 14-15 0.0 0.0 0.0 1.5299807375425143E-4 0.0 16-17 0.0 0.0 0.0 3.0599614750850286E-4 0.0 18-19 7.649903687712572E-5 0.0 0.0 5.3549325813988E-4 0.0 20-21 1.5299807375425143E-4 0.0 0.0 6.119922950170057E-4 0.0 22-23 1.5299807375425143E-4 0.0 0.0 8.797389240869458E-4 0.0 24-25 1.5299807375425143E-4 0.0 0.0 0.0011857350715954486 0.0 26-27 1.5299807375425143E-4 0.0 0.0 0.0017212283297353287 0.0 28-29 2.2949711063137716E-4 0.0 0.0 0.0034807061779092204 0.0 30-31 2.2949711063137716E-4 0.0 0.0 0.007573404650835445 0.0 32-33 2.2949711063137716E-4 0.0 0.0 0.0107098651627976 0.0 34-35 2.2949711063137716E-4 0.0 0.0 0.01445831796977676 0.0 36-37 2.2949711063137716E-4 0.0 0.0 0.019430755366789933 0.0 38-39 2.2949711063137716E-4 0.0 0.0 0.02727190664669532 0.0 40-41 2.2949711063137716E-4 0.0 0.0 0.039779499176105376 0.0 42-43 3.0599614750850286E-4 0.0 0.0 0.05201934507644548 0.0 44-45 4.589942212627543E-4 0.0 0.0 0.06678365919373075 0.0 46-47 5.354932581398801E-4 0.0 0.0 0.09386431824823326 0.0 48-49 7.649903687712571E-4 0.0 0.0 0.11142084721153361 0.0 50-51 7.649903687712571E-4 0.0 0.0 0.13123409776270917 0.0 52-53 7.649903687712571E-4 0.0 0.0 0.1543750564180397 0.0 54-55 7.649903687712571E-4 0.0 0.0 0.18520416827952135 0.0 56-57 7.649903687712571E-4 0.0 0.0 0.25500953942989857 0.0 58-59 7.649903687712571E-4 0.0 0.0 0.31972772462794696 0.0 60-61 7.649903687712571E-4 0.0 0.0 0.3611519530969105 0.0 62-63 7.649903687712571E-4 0.0 0.0 0.41531327120591555 0.0 64-65 7.649903687712571E-4 0.0 0.0 0.47058382534963883 0.0 66-67 8.414894056483829E-4 0.0 0.0 0.5591697100533504 0.0 68-69 8.414894056483829E-4 0.0 0.0 0.6692518241195343 0.0 70-71 8.414894056483829E-4 0.0 0.0 0.7190909466449817 0.0 72-73 8.414894056483829E-4 0.0 0.0 0.7792574391488412 0.0 74-75 8.414894056483829E-4 0.0 0.0 0.894503238204231 0.0 76-77 8.414894056483829E-4 0.0 0.0 1.0274968138151142 0.0 78-79 8.414894056483829E-4 0.0 0.0 1.157698174579982 0.0 80-81 0.001032736997841197 0.0 0.0 1.2597478897740677 0.0 82-83 0.00107098651627976 0.0 0.0 1.4297669992334798 0.0 84-85 0.00107098651627976 0.0 0.0 1.7187038615183834 0.0 86-87 0.00107098651627976 0.0 0.0 1.9714949288788455 0.0 88-89 0.00107098651627976 0.0 0.0 2.285140980075061 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 60 0.003939872 31.685158 1 GGTATCA 8130 0.0 29.639069 1 GTATCAA 11620 0.0 28.467562 1 GTACATG 8355 0.0 26.337934 1 TACATGG 8430 0.0 25.655523 2 TGTACGC 95 0.0012216584 25.017473 2 ACATGGG 8525 0.0 24.867811 3 GAGTACT 7000 0.0 24.3368 12-13 TATCAAC 13650 0.0 24.306356 2 ATCAACG 13665 0.0 24.279673 3 TCAACGC 13875 0.0 23.946457 4 CAACGCA 13975 0.0 23.774193 5 ATTCGCG 100 2.0547304E-8 23.752048 80-81 AACGCAG 14255 0.0 23.336985 6 TACCTGG 1000 0.0 22.815937 2 GTACTTT 7660 0.0 22.083115 14-15 ACGCAGA 15505 0.0 21.423283 7 AGTACTT 7235 0.0 21.313192 12-13 CGCAGAG 15570 0.0 21.242289 8 TACTACG 135 3.7784572E-4 21.125866 2 >>END_MODULE