FastQCFastQC Report
Thu 26 May 2016
SRR937012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307206
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96850.7408931721549625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78140.5977634741578604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51830.3964945081341426No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30300.23179208173769095No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25860.1978265093642471No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.16049497936820975No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17510.13394981357184713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.11199458998811206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89700.024.8418751
GTATCAA141050.024.825521
GGTATCA105550.023.8123041
TACATGG92600.023.7505862
GAGTACT78750.023.04227812-13
TACCTGG8700.022.9315972
ACATGGG92300.022.9005533
GTACTTT83250.021.93771714-15
AGTACTT80800.021.13489712-13
CATGGGA57300.020.9726624
TATCAAC168050.020.832112
ATCAACG168350.020.7659783
TCAACGC170450.020.5379984
CAACGCA171050.020.465965
AACGCAG175200.019.9827066
CATGGGG34650.019.4658244
GTATAGA4200.019.2310771
ACTTTTT97150.018.89706616-17
TACTTTT91000.018.71229714-15
ACGCAGA189400.018.4092867
Produced by FastQC (version 0.11.5)