FastQCFastQC Report
Thu 26 May 2016
SRR937011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833790
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129720.7073874325849743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89860.4900233941727243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.33973355727755083No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.11244471831561957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.048.7541351
GTATCAA98300.043.0361
ATCAACG129500.032.2672543
TCAACGC130300.032.069144
TATCAAC133950.031.3718832
CAACGCA132900.031.3676475
AACGCAG136750.030.4828686
GTACATG103800.027.0635991
ACGCAGA154950.026.6555777
CGCAGAG154650.026.465038
TACATGG107900.025.4646342
ACATGGG104200.025.1833933
GCAGAGT162850.024.548649
GAGTACT88850.022.88997712-13
CATGGGG34550.022.289994
AGTACTT96650.021.78015312-13
CATGGGA66850.021.4757544
GTACTTT94950.021.44532814-15
AGAGTAC150400.021.06088410-11
CAGAGTA159850.019.8158110-11
Produced by FastQC (version 0.11.5)