##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937011_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1833790 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.732478637139476 33.0 31.0 34.0 30.0 34.0 2 31.8324513712039 33.0 31.0 34.0 30.0 34.0 3 31.821423936219524 33.0 31.0 34.0 30.0 34.0 4 35.421473560222275 37.0 35.0 37.0 33.0 37.0 5 35.452509829369774 37.0 35.0 37.0 33.0 37.0 6 35.51853102045491 37.0 35.0 37.0 33.0 37.0 7 35.46054237399048 37.0 35.0 37.0 33.0 37.0 8 35.42893570147073 37.0 35.0 37.0 33.0 37.0 9 37.02826059690586 39.0 37.0 39.0 33.0 39.0 10-11 36.902627072892756 39.0 37.0 39.0 33.0 39.0 12-13 36.79574324213787 39.0 37.0 39.0 32.5 39.0 14-15 37.9141193920787 40.0 38.0 41.0 33.0 41.0 16-17 37.85352139557965 40.0 38.0 41.0 33.0 41.0 18-19 37.858868245546105 40.0 38.0 41.0 33.0 41.0 20-21 37.817466830989375 40.0 37.5 41.0 32.0 41.0 22-23 37.67136749573288 40.0 37.0 41.0 32.0 41.0 24-25 37.501834179486195 40.0 37.0 41.0 32.0 41.0 26-27 37.455425375860926 40.0 37.0 41.0 32.0 41.0 28-29 37.52297891252543 40.0 37.0 41.0 32.0 41.0 30-31 37.360559006211176 40.0 37.0 41.0 32.0 41.0 32-33 37.15441626358525 39.5 36.0 41.0 31.0 41.0 34-35 36.94966681026726 39.0 36.0 41.0 31.0 41.0 36-37 36.7826978552615 39.0 35.5 41.0 30.0 41.0 38-39 36.61830689446447 39.0 35.0 41.0 30.0 41.0 40-41 36.40654927772537 39.0 35.0 41.0 30.0 41.0 42-43 36.16340829647888 39.0 35.0 41.0 30.0 41.0 44-45 35.87257183210727 39.0 35.0 40.0 28.5 41.0 46-47 35.45935330654001 39.0 35.0 40.0 27.0 41.0 48-49 35.063850822613276 38.0 35.0 40.0 25.5 41.0 50-51 34.390543082904806 37.5 33.5 39.5 24.0 40.5 52-53 34.67957208840707 38.0 34.5 40.0 24.5 40.5 54-55 34.98232104003185 39.0 35.0 40.0 25.5 41.0 56-57 34.87567087834485 39.0 35.0 41.0 25.0 41.0 58-59 34.65233968993178 38.0 34.0 40.0 24.0 41.0 60-61 34.387898832472644 38.0 34.0 40.0 23.0 41.0 62-63 34.08675529913458 38.0 34.0 40.0 23.0 41.0 64-65 33.78196549223193 37.0 34.0 40.0 22.0 41.0 66-67 33.427070438817964 37.0 33.0 39.5 21.5 41.0 68-69 32.99232845636632 36.0 33.0 39.0 20.0 41.0 70-71 32.64834850228216 36.0 32.5 39.0 19.5 40.5 72-73 32.2783663887359 35.0 32.0 38.5 18.0 40.0 74-75 31.85023857693629 35.0 32.0 37.5 16.5 39.5 76-77 31.421169817699955 35.0 31.0 37.0 12.5 39.0 78-79 30.99626347618866 35.0 31.0 36.5 8.0 39.0 80-81 30.579607261463963 35.0 31.0 36.0 7.0 38.5 82-83 30.176822318804227 34.5 30.5 35.5 4.0 37.0 84-85 29.823141690160817 34.0 30.0 35.0 2.0 37.0 86-87 29.503528484722896 34.0 30.0 35.0 2.0 36.0 88-89 29.137638988106602 34.0 29.5 35.0 2.0 36.0 90-91 28.810142110056223 34.0 29.0 35.0 2.0 36.0 92-93 28.472668080859858 34.0 29.0 35.0 2.0 35.0 94-95 28.096134235654027 34.0 29.0 35.0 2.0 35.0 96-97 27.668005333217 34.0 28.0 35.0 2.0 35.0 98-99 27.09191128755201 34.0 27.0 35.0 2.0 35.0 100-101 24.924269681915597 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39424769068618915 1101 2 0.36094076912824846 1101 3 0.4956964511078681 1101 4 0.4406436633390669 1101 5 0.27825432443424347 1101 6 0.2113798837201557 1101 7 0.3109984222153912 1101 8 0.2530125407073953 1101 9 0.3879855970751933 1101 10-11 0.32275583381647976 1101 12-13 0.5371659131453157 1101 14-15 0.6665050232061773 1101 16-17 0.6673073907816445 1101 18-19 0.6637075904631473 1101 20-21 0.5922466758771137 1101 22-23 0.5994598130754198 1101 24-25 0.6519703264631218 1101 26-27 0.6402146236813238 1101 28-29 0.5019515170574138 1101 30-31 0.5730001899283295 1101 32-33 0.666818171402987 1101 34-35 0.7538887594598052 1101 36-37 0.649205592158431 1101 38-39 0.7589444026465699 1101 40-41 0.7433496829097308 1101 42-43 0.820105358561058 1101 44-45 0.8629504341996395 1101 46-47 0.7782782970842774 1101 48-49 0.7885073838397005 1101 50-51 0.8168311641626573 1101 52-53 0.8356476183999604 1101 54-55 0.6952728649823712 1101 56-57 0.7825828235294878 1101 58-59 0.7597087092856256 1101 60-61 0.881888997499999 1101 62-63 0.8741707061675186 1101 64-65 0.806951152854495 1101 66-67 0.7715603532346762 1101 68-69 0.7177424016313694 1101 70-71 0.8291205422706653 1101 72-73 0.9052930780105797 1101 74-75 0.7341825660985641 1101 76-77 0.7893303973579897 1101 78-79 0.9281244716177817 1101 80-81 0.9536447896966109 1101 82-83 0.7016916885456119 1101 84-85 0.9016991684816382 1101 86-87 1.002001804140825 1101 88-89 0.9668115182546373 1101 90-91 0.9744331927107304 1101 92-93 0.8599147292504625 1101 94-95 1.1224150736219833 1101 96-97 1.2209036026085442 1101 98-99 1.3810225648166856 1101 100-101 1.586186669337021 1102 1 0.3984467976303314 1102 2 0.4436020715782405 1102 3 0.43110454194532366 1102 4 0.2866546871853046 1102 5 0.23466070695649677 1102 6 0.3455645842737525 1102 7 0.30787829076334816 1102 8 0.3534504969849479 1102 9 0.44376549710266033 1102 10-11 0.5325913784021026 1102 12-13 0.5341111969321588 1102 14-15 0.5825666197991737 1102 16-17 0.5283016372482194 1102 18-19 0.5415762116001375 1102 20-21 0.5662411108197816 1102 22-23 0.6043472838265345 1102 24-25 0.5958042008297681 1102 26-27 0.5602303333639824 1102 28-29 0.582884704288368 1102 30-31 0.6386297201215996 1102 32-33 0.6183746781390127 1102 34-35 0.45991129739061165 1102 36-37 0.49926346334104466 1102 38-39 0.5143011838759577 1102 40-41 0.453458292256947 1102 42-43 0.550170252483106 1102 44-45 0.5750023272815596 1102 46-47 0.5337542543523384 1102 48-49 0.39361413348931507 1102 50-51 0.1344574882573255 1102 52-53 0.2063171797118315 1102 54-55 0.3477220766911415 1102 56-57 0.5226771768660186 1102 58-59 0.3633167983185288 1102 60-61 0.40381663671665535 1102 62-63 0.5618980303884982 1102 64-65 0.678576879439845 1102 66-67 0.4541748875483549 1102 68-69 0.2649346091475593 1102 70-71 0.46795097795119034 1102 72-73 0.5057425634106991 1102 74-75 0.547964053134816 1102 76-77 0.6081001096512431 1102 78-79 0.6187665334660117 1102 80-81 0.5788253708445907 1102 82-83 0.5245716583493341 1102 84-85 0.6539074967187659 1102 86-87 0.6837905023926076 1102 88-89 0.68919438236939 1102 90-91 0.5786811480459058 1102 92-93 0.7059145999237835 1102 94-95 0.5606040640117058 1102 96-97 0.3041443687839056 1102 98-99 0.40270370638027586 1102 100-101 0.7902919893754401 1103 1 0.345324979751215 1103 2 0.36357524012852593 1103 3 0.41325998237989126 1103 4 0.319377053308763 1103 5 0.2420412915181558 1103 6 0.24024388456529522 1103 7 0.16960291800752714 1103 8 0.15174081481954005 1103 9 0.3353091378942139 1103 10-11 0.42347391240603116 1103 12-13 0.21810892806787052 1103 14-15 0.3916096023877742 1103 16-17 0.4592859120053632 1103 18-19 0.5490325378984409 1103 20-21 0.4649487583637111 1103 22-23 0.4164063846438708 1103 24-25 0.5875413339300195 1103 26-27 0.6943309571080789 1103 28-29 0.6495979291343659 1103 30-31 0.6652458019555851 1103 32-33 0.5373699797314728 1103 34-35 0.5769884213534553 1103 36-37 0.6899189657241251 1103 38-39 0.7006979679737739 1103 40-41 0.7438417051553969 1103 42-43 0.8206350405611147 1103 44-45 0.9098016140993082 1103 46-47 0.7477053014757971 1103 48-49 0.7643706603459322 1103 50-51 0.7192988807050256 1103 52-53 0.8141893838012066 1103 54-55 0.7694058469550811 1103 56-57 0.802129581049094 1103 58-59 0.8352258095041023 1103 60-61 0.697247868059506 1103 62-63 0.7430889615133722 1103 64-65 0.7921294717915259 1103 66-67 0.7626286333039118 1103 68-69 1.00451177182822 1103 70-71 1.018920568974849 1103 72-73 0.88141141376245 1103 74-75 0.8623050855067866 1103 76-77 0.9021968035365369 1103 78-79 0.9222000298013704 1103 80-81 0.8664414275119938 1103 82-83 0.8650114056768352 1103 84-85 0.9078039613945634 1103 86-87 0.9918498103502813 1103 88-89 0.989595547388614 1103 90-91 1.1801327275394016 1103 92-93 1.0011871056249824 1103 94-95 0.8935920827821064 1103 96-97 0.8967786905831971 1103 98-99 1.2559019435579053 1103 100-101 1.643845220571162 1104 1 0.3013493699095484 1104 2 0.37392667312049355 1104 3 0.23902577090262156 1104 4 0.32131542535690727 1104 5 0.2362279295565557 1104 6 0.20032219128767537 1104 7 0.09757792441029522 1104 8 0.2250253117023533 1104 9 0.16135208730708683 1104 10-11 0.17919167140235004 1104 12-13 0.2879683829380113 1104 14-15 0.3390367424031382 1104 16-17 0.44576291642238886 1104 18-19 0.43018051023848614 1104 20-21 0.36425778573948975 1104 22-23 0.5076580993576059 1104 24-25 0.22262569258322884 1104 26-27 0.338935266082828 1104 28-29 0.3908691152777237 1104 30-31 0.4226984477895783 1104 32-33 0.4422099456301609 1104 34-35 0.43689236230064665 1104 36-37 0.4995217134761418 1104 38-39 0.4659286605602446 1104 40-41 0.4455622076937047 1104 42-43 0.5944349163649676 1104 44-45 0.44540801756837567 1104 46-47 0.534048461741456 1104 48-49 0.48217351124390007 1104 50-51 0.519056277217004 1104 52-53 0.5777553568639746 1104 54-55 0.5079238431169273 1104 56-57 0.5451444249443611 1104 58-59 0.6657559586447306 1104 60-61 0.8107189398301458 1104 62-63 0.629618766834156 1104 64-65 0.7015145254519837 1104 66-67 0.5504198715114157 1104 68-69 0.738468015491236 1104 70-71 0.7931414486624604 1104 72-73 0.7619392824653701 1104 74-75 0.5806298058023351 1104 76-77 0.6773851150653805 1104 78-79 0.6982233323663358 1104 80-81 0.7402946304744589 1104 82-83 0.8221154953787284 1104 84-85 0.7276072824135333 1104 86-87 0.623160921695284 1104 88-89 0.4510657982016788 1104 90-91 0.4390851997092895 1104 92-93 0.36681933066559225 1104 94-95 0.4707436757555534 1104 96-97 0.4815785207782959 1104 98-99 0.5066711527371908 1104 100-101 0.5960963634212924 1105 1 0.26443397673346425 1105 2 0.3329629952305666 1105 3 0.3173313853771802 1105 4 0.27705185255176445 1105 5 0.26991710349707887 1105 6 0.28957639952616887 1105 7 0.30660358293515344 1105 8 0.332626703009403 1105 9 0.35819799268424646 1105 10-11 0.30324988294461974 1105 12-13 0.3424631810472576 1105 14-15 0.42521402159906785 1105 16-17 0.5498055785185798 1105 18-19 0.46172242588370693 1105 20-21 0.5376816109032205 1105 22-23 0.41167516884928546 1105 24-25 0.4632126550900608 1105 26-27 0.4452899873367073 1105 28-29 0.5566742935147033 1105 30-31 0.349776005014256 1105 32-33 0.4459935795473413 1105 34-35 0.3562708110010462 1105 36-37 0.3495655311172001 1105 38-39 0.337123214764226 1105 40-41 0.5988619087255458 1105 42-43 0.6000197267664262 1105 44-45 0.5562647106161123 1105 46-47 0.7926646385941041 1105 48-49 0.7677336596809994 1105 50-51 0.6839298458730383 1105 52-53 0.5140384963785749 1105 54-55 0.6526471133864717 1105 56-57 0.8522421072625832 1105 58-59 0.7974783797051188 1105 60-61 0.594324743922634 1105 62-63 0.5819079477544804 1105 64-65 0.7742275741903839 1105 66-67 0.6655875612359878 1105 68-69 0.7533097872750005 1105 70-71 0.6486198170951454 1105 72-73 0.6161564395923378 1105 74-75 0.6933623204308326 1105 76-77 0.48577309119446 1105 78-79 0.6170366110504126 1105 80-81 0.69522256402675 1105 82-83 0.7299288012064764 1105 84-85 0.756520139595164 1105 86-87 0.736594324697375 1105 88-89 0.7826751852587996 1105 90-91 0.8279512534460061 1105 92-93 0.8978164340480816 1105 94-95 1.0491084246572022 1105 96-97 0.8185090368570442 1105 98-99 0.9784097436704293 1105 100-101 1.0013077544568993 1106 1 0.6924213310625653 1106 2 0.5790688358405518 1106 3 0.6686076736847042 1106 4 0.42184266523611313 1106 5 0.45028280549207267 1106 6 0.579072599070301 1106 7 0.646821531364445 1106 8 0.4903154907705982 1106 9 0.7895414561517953 1106 10-11 0.7887394126522764 1106 12-13 0.7779140373583715 1106 14-15 0.9503590137253184 1106 16-17 0.9110422530832736 1106 18-19 0.9324243480309917 1106 20-21 1.0124362930992916 1106 22-23 1.079932919090659 1106 24-25 1.0653716769180122 1106 26-27 1.1951757551294548 1106 28-29 1.1508109033146994 1106 30-31 1.0157752686303994 1106 32-33 1.0939130302791469 1106 34-35 0.9720486296890343 1106 36-37 1.02737143834608 1106 38-39 1.1216332612385216 1106 40-41 1.0321377175889026 1106 42-43 1.0456451961117992 1106 44-45 1.2445025016764646 1106 46-47 1.0899177016727961 1106 48-49 1.1192178837165159 1106 50-51 1.0003146514663825 1106 52-53 0.9246724884399242 1106 54-55 0.8850275404096379 1106 56-57 0.9291551490709082 1106 58-59 1.0580781352150908 1106 60-61 1.1451822470251116 1106 62-63 1.1034405532686193 1106 64-65 1.0170994844575034 1106 66-67 1.1641138002084332 1106 68-69 1.0689515868593986 1106 70-71 1.1355880744480586 1106 72-73 1.0438951319282452 1106 74-75 1.1699400824331612 1106 76-77 1.1820349498220075 1106 78-79 1.2258204386836162 1106 80-81 1.448027047491891 1106 82-83 1.3207338038487144 1106 84-85 1.4796004094083308 1106 86-87 1.3281108781030824 1106 88-89 1.413472055892317 1106 90-91 1.5795822907009374 1106 92-93 1.5716983821806991 1106 94-95 1.6236782162314363 1106 96-97 1.5510421045977374 1106 98-99 1.627013925430095 1106 100-101 1.819867276390145 1107 1 0.3348035032433394 1107 2 0.5294058821496463 1107 3 0.4370040639471 1107 4 0.39617843283122767 1107 5 0.35326038232908274 1107 6 0.31456009493592774 1107 7 0.3972944756310568 1107 8 0.45996886794554825 1107 9 0.4681192605002522 1107 10-11 0.5435162535368079 1107 12-13 0.4901065197933505 1107 14-15 0.4825064228764333 1107 16-17 0.6176929496640966 1107 18-19 0.5600162002426501 1107 20-21 0.6282263349065929 1107 22-23 0.6482846097193757 1107 24-25 0.36523321876476444 1107 26-27 0.5799354634608846 1107 28-29 0.5009671444491559 1107 30-31 0.5376268852210231 1107 32-33 0.569529933660128 1107 34-35 0.6739460115083276 1107 36-37 0.587294895412974 1107 38-39 0.6809270485429906 1107 40-41 0.6969302517813603 1107 42-43 0.7316704022767411 1107 44-45 0.8162388402644254 1107 46-47 0.6909333382412512 1107 48-49 0.8699797149345727 1107 50-51 0.896271064479933 1107 52-53 0.8193225096826353 1107 54-55 0.8338893604181834 1107 56-57 0.9328169209200965 1107 58-59 0.9244317660570047 1107 60-61 0.9121307428063261 1107 62-63 0.9466527824241311 1107 64-65 1.0167337916339392 1107 66-67 0.8895137788965357 1107 68-69 1.0038655296853847 1107 70-71 0.9573296026219751 1107 72-73 0.8779020735184204 1107 74-75 0.9426158052732951 1107 76-77 0.9712457314290042 1107 78-79 1.1867985580053393 1107 80-81 1.252938402444343 1107 82-83 1.176858414802755 1107 84-85 1.127608092763321 1107 86-87 1.226575590994429 1107 88-89 1.0151204759747685 1107 90-91 0.9645291700991088 1107 92-93 1.129678858266395 1107 94-95 1.1202741938179592 1107 96-97 1.2061200798565572 1107 98-99 1.4842152024834085 1107 100-101 1.6179750841568108 1108 1 0.0319744421983188 1108 2 0.03968685487193113 1108 3 0.03577739980963912 1108 4 0.05833934616585168 1108 5 0.05794719264969217 1108 6 0.06861442176020915 1108 7 0.104177924298547 1108 8 0.058078895633045136 1108 9 -0.012670945642064169 1108 10-11 -7.961980526403067E-4 1108 12-13 -0.09100372798355494 1108 14-15 0.0927757831457896 1108 16-17 0.2305179615501416 1108 18-19 0.31807773529948946 1108 20-21 0.2997456085385508 1108 22-23 0.25367278787751246 1108 24-25 0.333732768250222 1108 26-27 0.33369066322546814 1108 28-29 0.2821596521674863 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-1.0745339225934494 2316 70-71 -1.5649475014807166 2316 72-73 -1.395003839729796 2316 74-75 -1.6897492744838054 2316 76-77 -2.0117943954579047 2316 78-79 -2.2499303172728418 2316 80-81 -2.1812595949158364 2316 82-83 -2.099603158276583 2316 84-85 -1.9086235526777777 2316 86-87 -1.9155592867150197 2316 88-89 -2.3089342522364404 2316 90-91 -2.167698979366204 2316 92-93 -2.478517476172808 2316 94-95 -2.4829000369438816 2316 96-97 -2.6390905156392854 2316 98-99 -2.7310260445426806 2316 100-101 -2.258581708985151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4270.0 3 1161.0 4 1222.0 5 1369.0 6 1570.0 7 1838.0 8 2244.0 9 2921.0 10 3641.0 11 4947.0 12 6717.0 13 8290.0 14 9607.0 15 12228.0 16 16181.0 17 21603.0 18 17958.0 19 11529.0 20 10580.0 21 10784.0 22 11618.0 23 12752.0 24 14450.0 25 16654.0 26 19370.0 27 22803.0 28 27325.0 29 33163.0 30 40788.0 31 50277.0 32 64492.0 33 84678.0 34 115015.0 35 163542.0 36 244416.0 37 358598.0 38 339611.0 39 63565.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0565339115622 19.99358331837381 15.057048992128106 21.89283377793589 2 14.87798448013021 21.253390229697786 40.80965971891318 23.05896557125882 3 17.612734729871022 27.376329581147836 30.78745691753268 24.223478771448466 4 11.921248647273307 17.81422298614349 39.18113488172992 31.083393484853282 5 12.616183445793434 38.37270301090284 34.56519947224453 14.445914071059207 6 29.001027853576446 38.2675399692026 18.488973363690402 14.242458813530549 7 26.912243006265786 34.09076269178657 22.204389651140318 16.792604650807323 8 25.914988941313638 34.30845547377683 20.8546301289707 18.921925455938833 9 26.973531200557304 16.62273580445343 21.41405692959306 34.9896760653962 10-11 23.712402451836297 27.54762053583492 28.28261461150099 20.457362400827797 12-13 25.203439289960787 25.393622516995464 28.64521263052412 20.75772556251963 14-15 22.522887382561105 26.161726041319277 27.476130150650363 23.83925642546925 16-17 20.780657486282237 29.675318347858344 27.956254925033047 21.587769240826372 18-19 20.649367554599944 27.483287291573827 31.347984282399405 20.519360871426827 20-21 21.57089479546215 26.702608517230857 31.387309228882778 20.339187458424217 22-23 21.320133796201176 26.728134992388764 31.248463496496704 20.703267714913352 24-25 21.030791480431724 27.10524944501237 31.31311560521275 20.550843469343157 26-27 20.84616887148748 27.205748356887433 31.23717128200428 20.71091148962081 28-29 20.525363302436404 26.77045735877226 31.886880841602782 20.817298497188556 30-31 20.758478895815607 26.742951200503462 31.73711760779325 20.76145229588768 32-33 20.648581379971063 27.060359821660445 31.463663206227483 20.827395592141013 34-35 21.0319780010251 27.039688335125938 31.122515643118405 20.80581802073056 36-37 21.3808146711627 26.621953658038233 30.87219010357774 21.125041567221324 38-39 20.690161250767574 27.25855690779127 31.068577200874792 20.982704640566364 40-41 20.789225839824866 26.898977651960777 31.434337492715443 20.87745901549892 42-43 20.973897518904295 26.95711316276886 30.75810605938664 21.3108832589402 44-45 20.82453602015553 27.214767920058748 30.57455047775548 21.386145582030238 46-47 21.211202113952236 27.38392410021199 30.051621413149142 21.353252372686637 48-49 21.28766170443464 27.610640953075876 29.600343696510528 21.501353645978956 50-51 21.30862873612623 27.70466193498557 29.253712944195108 21.73299638469309 52-53 21.36106061205313 27.986067260653787 29.21090997862901 21.441962148664075 54-55 21.312080446290988 27.827037419233054 29.151933101087877 21.70894903338808 56-57 21.26610600549786 28.328865448139297 28.899710867313495 21.50531767904935 58-59 21.3211551787166 27.636663229284313 29.28728877831066 21.75489281368842 60-61 21.48538234084965 28.003455849396115 29.30717421567523 21.203987594079006 62-63 21.238383724296053 27.963007978034828 29.544783039630452 21.25382525803867 64-65 21.18277742605186 27.776415427071488 29.367010642792827 21.673796504083825 66-67 21.272213717674042 28.232568760658914 28.84197700689073 21.653240514776314 68-69 21.03974974554718 27.968914062736566 29.42601281225542 21.565323379460835 70-71 21.213513752330417 27.95059022713463 29.33482312822018 21.501072892314774 72-73 21.327002068787596 28.004746266719245 28.910474893928956 21.757776770564202 74-75 20.979493785297173 28.522094493811206 28.722037382856403 21.776374338035215 76-77 21.062857672814957 28.27992449659853 29.068045803942105 21.58917202664441 78-79 21.100078682670933 28.197206492357086 28.835834772938835 21.866880052033146 80-81 21.01530748626636 28.83630918285518 28.510002236660505 21.63838109421796 82-83 21.414462627735315 28.537029600108255 28.483911134197925 21.564596637958505 84-85 21.0655293570057 28.314759834096265 28.39420853866222 22.22550227023582 86-87 21.03923812849829 28.24242159672704 28.779229424981278 21.939110849793394 88-89 21.321643800924637 28.109921484264245 28.708061241782673 21.86037347302845 90-91 21.06122290848512 28.17261085292129 28.997435926817783 21.7687303117758 92-93 21.21386335340004 27.933094730259107 28.872099784362266 21.980942131978587 94-95 20.96392781633836 27.658478895131104 29.024638628715632 22.352954659814902 96-97 21.27002753572959 27.739614063143875 28.800217229343428 22.19014117178311 98-99 20.909518007923687 27.876145209930243 28.809456667994287 22.40488011415178 100-101 20.773924804928747 27.873849601668393 28.935812447709168 22.416413145693692 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 747.0 1 580.0 2 463.5 3 557.5 4 557.0 5 592.0 6 964.5 7 1977.0 8 3383.0 9 3985.0 10 3515.0 11 3043.5 12 3110.0 13 3356.0 14 3531.5 15 3644.0 16 3763.5 17 3889.5 18 3942.5 19 4202.5 20 4678.0 21 5252.5 22 5960.5 23 7016.5 24 8335.5 25 9966.0 26 12877.0 27 15992.0 28 17399.0 29 19788.0 30 24679.5 31 29487.5 32 35373.5 33 42768.5 34 50077.5 35 55753.5 36 64356.0 37 72415.5 38 71921.0 39 68930.5 40 65565.0 41 63112.0 42 61260.5 43 61826.0 44 63559.5 45 63413.5 46 59729.0 47 55254.5 48 56771.0 49 56494.0 50 52785.5 51 49520.0 52 47864.5 53 48132.5 54 47499.0 55 44624.0 56 40558.0 57 36049.0 58 32781.5 59 30096.0 60 26456.5 61 22562.0 62 18521.5 63 14629.0 64 11245.5 65 8612.5 66 6259.0 67 5078.5 68 3745.5 69 2086.5 70 1402.0 71 1025.5 72 825.0 73 626.5 74 433.5 75 354.5 76 241.0 77 107.0 78 59.5 79 43.0 80 29.5 81 21.5 82 17.5 83 9.5 84 8.5 85 6.5 86 4.5 87 6.5 88 4.5 89 2.5 90 3.5 91 1.5 92 1.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05818550651928519 2 0.091995266633584 3 0.06860109390933532 4 0.12564143113442652 5 0.10257444963709039 6 0.09908440988335633 7 0.10557370255045562 8 0.0952126470315576 9 0.11582569432704944 10-11 0.10571003222833585 12-13 0.08776904661929665 14-15 0.09090462921054211 16-17 0.1063098828110089 18-19 0.08596949487127752 20-21 0.07050970939965863 22-23 0.07097323030445143 24-25 0.05952153736251152 26-27 0.05387748869826971 28-29 0.064974724477721 30-31 0.047524525709050656 32-33 0.04809711035614765 34-35 0.04338010350149145 36-37 0.04992392803974283 38-39 0.049051418101309315 40-41 0.06249352434030069 42-43 0.05843089993946962 44-45 0.046024899252368044 46-47 0.03468227005273232 48-49 0.04239852982075374 50-51 0.025848106926092955 52-53 0.03803598012858615 54-55 0.04447074092453335 56-57 0.048778758745548836 58-59 0.054531871152094846 60-61 0.05210520288582662 62-63 0.05837636806831752 64-65 0.0417441473669286 66-67 0.06055764291440132 68-69 0.05101456546278472 70-71 0.02333964085309659 72-73 0.0190316230320811 74-75 0.028165711450056988 76-77 0.041444222075592084 78-79 0.060639440721129464 80-81 0.03817230980646639 82-83 0.059657867040391756 84-85 0.06331150240758211 86-87 0.06530191570463358 88-89 0.08776904661929665 90-91 0.06320243866527793 92-93 0.07190027211403704 94-95 0.07983465936666685 96-97 0.08861429062215412 98-99 0.11961565937212003 100-101 0.11383528102999799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1833790.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.013761964278615 #Duplication Level Percentage of deduplicated Percentage of total 1 75.11096985994648 32.30805378461844 2 13.104724557749028 11.273670054689063 3 4.58057849995982 5.910837397678924 4 2.1132999594334336 3.6360392565675745 5 1.1827687864827974 2.5437667520274867 6 0.7437341802701096 1.9194482996901827 7 0.4818692828948647 1.4508907442636143 8 0.34709632713925537 1.1943935035394648 9 0.27231465152505047 1.0541949840075604 >10 1.6603736044944635 13.963838820471159 >50 0.22687621238800357 6.885552290034798 >100 0.16479919958127487 13.0907225828144 >500 0.008807790257255456 2.468879528012325 >1k 0.0014041404757943478 0.7520020908630446 >5k 2.5529826832624506E-4 0.8354602668349945 >10k+ 1.2764913416312253E-4 0.7122496438869316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12972 0.7073874325849743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8986 0.4900233941727243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 0.33973355727755083 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.11244471831561957 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.453187115209484E-5 2 0.0 0.0 0.0 0.0 5.453187115209484E-5 3 0.0 0.0 0.0 0.0 5.453187115209484E-5 4 0.0 0.0 0.0 0.0 5.453187115209484E-5 5 0.0 0.0 0.0 0.0 5.453187115209484E-5 6 0.0 0.0 0.0 0.0 5.453187115209484E-5 7 0.0 0.0 0.0 0.0 5.453187115209484E-5 8 0.0 0.0 0.0 0.0 5.453187115209484E-5 9 0.0 0.0 0.0 5.453187115209484E-5 5.453187115209484E-5 10-11 0.0 0.0 0.0 5.453187115209484E-5 5.453187115209484E-5 12-13 0.0 0.0 0.0 5.453187115209484E-5 5.453187115209484E-5 14-15 0.0 0.0 0.0 5.453187115209484E-5 5.453187115209484E-5 16-17 0.0 0.0 0.0 5.453187115209484E-5 5.453187115209484E-5 18-19 0.0 0.0 0.0 5.453187115209484E-5 5.453187115209484E-5 20-21 0.0 0.0 0.0 8.179780672814226E-5 5.453187115209484E-5 22-23 0.0 0.0 0.0 1.9086154903233196E-4 5.453187115209484E-5 24-25 0.0 0.0 0.0 3.817230980646639E-4 5.453187115209484E-5 26-27 0.0 0.0 0.0 8.179780672814226E-4 5.453187115209484E-5 28-29 0.0 0.0 0.0 0.0022903385883879836 5.453187115209484E-5 30-31 0.0 0.0 0.0 0.005344123372905294 5.453187115209484E-5 32-33 0.0 0.0 0.0 0.00782532351032561 1.0906374230418968E-4 34-35 0.0 0.0 0.0 0.01003386429198545 1.0906374230418968E-4 36-37 0.0 0.0 0.0 0.013387574367839283 1.0906374230418968E-4 38-39 0.0 0.0 0.0 0.01924975051668948 1.0906374230418968E-4 40-41 0.0 0.0 0.0 0.026502489379918094 1.0906374230418968E-4 42-43 0.0 0.0 0.0 0.03348256888738624 1.6359561345628452E-4 44-45 0.0 0.0 0.0 0.035909237153654454 2.1812748460837937E-4 46-47 0.0 0.0 0.0 0.04043538245927833 2.1812748460837937E-4 48-49 0.0 0.0 0.0 0.04708827073983389 2.1812748460837937E-4 50-51 0.0 0.0 0.0 0.054450073345366695 2.1812748460837937E-4 52-53 0.0 0.0 0.0 0.06813757300454251 2.453934201844268E-4 54-55 0.0 0.0 0.0 0.07890761755708123 2.726593557604742E-4 56-57 0.0 0.0 0.0 0.105791830035064 2.726593557604742E-4 58-59 0.0 0.0 0.0 0.15508864155655772 2.726593557604742E-4 60-61 0.0 0.0 0.0 0.1947878437552828 2.726593557604742E-4 62-63 0.0 0.0 0.0 0.21512823169501416 2.726593557604742E-4 64-65 0.0 0.0 0.0 0.25651792189945416 2.726593557604742E-4 66-67 0.0 0.0 0.0 0.34202389586593884 2.726593557604742E-4 68-69 0.0 0.0 0.0 0.46649289177059533 2.726593557604742E-4 70-71 0.0 0.0 0.0 0.5677313105644594 3.2719122691256904E-4 72-73 0.0 0.0 0.0 0.6897463722672716 3.817230980646639E-4 74-75 0.0 0.0 0.0 0.8346648198539637 3.817230980646639E-4 76-77 0.0 0.0 0.0 0.9239334929299429 3.817230980646639E-4 78-79 0.0 0.0 0.0 1.0356420309850092 3.817230980646639E-4 80-81 0.0 0.0 0.0 1.1956658068808315 4.6352090479280614E-4 82-83 0.0 0.0 0.0 1.3411295731790445 4.907868403688536E-4 84-85 0.0 0.0 0.0 1.543170155797556 4.907868403688536E-4 86-87 0.0 5.453187115209484E-5 0.0 1.856592085244221 4.907868403688536E-4 88-89 0.0 5.453187115209484E-5 0.0 2.250312194962346 4.907868403688536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5255 0.0 48.754135 1 GTATCAA 9830 0.0 43.036 1 ATCAACG 12950 0.0 32.267254 3 TCAACGC 13030 0.0 32.06914 4 TATCAAC 13395 0.0 31.371883 2 CAACGCA 13290 0.0 31.367647 5 AACGCAG 13675 0.0 30.482868 6 GTACATG 10380 0.0 27.063599 1 ACGCAGA 15495 0.0 26.655577 7 CGCAGAG 15465 0.0 26.46503 8 TACATGG 10790 0.0 25.464634 2 ACATGGG 10420 0.0 25.183393 3 GCAGAGT 16285 0.0 24.54864 9 GAGTACT 8885 0.0 22.889977 12-13 CATGGGG 3455 0.0 22.28999 4 AGTACTT 9665 0.0 21.780153 12-13 CATGGGA 6685 0.0 21.475754 4 GTACTTT 9495 0.0 21.445328 14-15 AGAGTAC 15040 0.0 21.060884 10-11 CAGAGTA 15985 0.0 19.81581 10-11 >>END_MODULE