FastQCFastQC Report
Thu 26 May 2016
SRR937011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833790
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150400.8201593421275064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111110.6059036203709258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78090.4258393818267086No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25550.13932893079360234No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.11369895135211776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.051.8998221
GTATCAA112050.042.5639231
ATCAACG148000.032.092153
TCAACGC149450.031.779924
TATCAAC150050.031.6537062
CAACGCA152300.031.2164065
AACGCAG155550.030.5641846
ACGCAGA176850.026.8024357
CGCAGAG177800.026.5790868
GCAGAGT186700.025.0322289
GTACATG99500.024.9688431
ATACCGA1000.001655014823.7475476
GAGTACT113950.023.71628612-13
ACATGGG101400.023.2797873
TACATGG105500.023.005362
GTACTTT122250.021.99014914-15
AGTACTT123050.021.9237812-13
AGAGTAC175900.021.37144310-11
TAGGACA10550.020.708764
CAGAGTA183400.020.691710-11
Produced by FastQC (version 0.11.5)