FastQCFastQC Report
Thu 26 May 2016
SRR937010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651882
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122170.739580672227193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.5011253830479417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59200.3583791094036983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.11387011905208726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.051.4102251
GTATCAA100450.044.1462251
ATCAACG126850.034.8461153
TCAACGC127750.034.6750344
CAACGCA130200.034.026675
TATCAAC130000.033.9296722
AACGCAG133050.033.4713676
ACGCAGA150050.029.5172567
CGCAGAG150200.029.3638
GCAGAGT155250.027.520139
GTACATG97100.026.383441
ACATGGG97650.024.7259713
TACATGG101600.024.6542592
GAGTACT86550.024.43294712-13
GTACCGT1000.001648304923.7639566
GTACTTT92400.022.75782614-15
AGTACTT92250.022.38238112-13
GTATAGG7700.022.2215141
CATGGGG33000.022.1803634
ATGGGAT28700.022.0284675
Produced by FastQC (version 0.11.5)