FastQCFastQC Report
Thu 26 May 2016
SRR937010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651882
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139070.8418882220400732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102020.6175985936041436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71460.4325974857768291No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24600.1489210488400503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.11768395079067391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.053.730731
GTATCAA103350.041.0584451
ATCAACG136300.031.085623
TCAACGC137650.030.881474
TATCAAC138950.030.4936922
CAACGCA140250.030.2751145
AACGCAG144150.029.4871796
ACGCAGA164100.025.7576527
CGCAGAG164100.025.699778
GTACATG96350.025.0537951
GCAGAGT171650.024.265029
ACATGGG96150.023.3669723
TACATGG101600.023.2830242
GAGTACT103600.023.22045912-13
CATGGGG29800.022.4726334
GTACTTT109100.021.94268614-15
AGTACTT110200.021.39876712-13
CAGAGTA169500.020.86026610-11
ATGGGAT27150.020.6425485
GTATAGG8750.020.6365491
Produced by FastQC (version 0.11.5)