##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937010_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1651882 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.249669165230934 33.0 31.0 34.0 31.0 34.0 2 32.32565461697627 33.0 31.0 34.0 31.0 34.0 3 32.44844970766677 34.0 31.0 34.0 31.0 34.0 4 36.12531161426785 37.0 35.0 37.0 35.0 37.0 5 36.04839449791208 37.0 35.0 37.0 35.0 37.0 6 36.09024857707754 37.0 35.0 37.0 35.0 37.0 7 36.046202452717566 37.0 35.0 37.0 35.0 37.0 8 36.03363375834351 37.0 35.0 37.0 35.0 37.0 9 37.726602747653885 39.0 37.0 39.0 35.0 39.0 10-11 37.63405134265038 39.0 37.0 39.0 35.0 39.0 12-13 37.56788832374225 39.0 37.0 39.0 35.0 39.0 14-15 38.786439951521956 40.0 38.0 41.0 35.0 41.0 16-17 38.73133310974997 40.0 38.0 41.0 35.0 41.0 18-19 38.804857429283686 40.0 38.0 41.0 35.0 41.0 20-21 38.90285686265726 40.0 38.0 41.0 35.0 41.0 22-23 38.831314524887375 40.0 38.0 41.0 35.0 41.0 24-25 38.74428348998294 40.0 38.0 41.0 35.0 41.0 26-27 38.58064831507335 40.0 38.0 41.0 34.0 41.0 28-29 38.42988179543091 40.0 38.0 41.0 34.0 41.0 30-31 38.31018377826019 40.0 38.0 41.0 34.0 41.0 32-33 38.17697420275782 40.0 37.0 41.0 34.0 41.0 34-35 38.044242264277955 40.0 37.0 41.0 34.0 41.0 36-37 37.85931743308541 40.0 37.0 41.0 33.0 41.0 38-39 37.700979246701635 40.0 37.0 41.0 33.0 41.0 40-41 37.46248884605559 40.0 37.0 41.0 33.0 41.0 42-43 37.20168480557328 40.0 36.0 41.0 32.5 41.0 44-45 36.889370729870535 40.0 36.0 41.0 31.5 41.0 46-47 36.425555517888085 40.0 36.0 41.0 30.5 41.0 48-49 35.97195047830293 39.0 35.5 41.0 29.5 41.0 50-51 35.70816862221393 39.0 35.0 41.0 28.0 41.0 52-53 35.48527225310282 39.0 35.0 40.5 27.5 41.0 54-55 35.21671856706472 39.0 35.0 40.0 27.0 41.0 56-57 34.98552287633136 38.0 35.0 40.0 26.0 41.0 58-59 34.66262117996322 38.0 34.0 40.0 26.0 41.0 60-61 34.35761634305598 38.0 34.0 40.0 25.5 41.0 62-63 34.036713881500006 37.5 34.0 40.0 24.5 41.0 64-65 33.64498311622743 37.0 33.5 39.5 23.0 41.0 66-67 33.234054551112 36.0 33.0 39.0 22.0 40.5 68-69 32.80695776090544 36.0 32.5 39.0 21.5 40.0 70-71 32.44123006364862 35.0 32.0 38.0 20.0 40.0 72-73 32.07003557154809 35.0 32.0 38.0 19.5 40.0 74-75 31.598870863657332 35.0 31.0 37.0 18.0 39.0 76-77 30.12830698560793 33.5 29.5 35.5 14.0 38.5 78-79 30.813543279725792 34.0 31.0 36.0 14.0 38.5 80-81 30.513163167829177 34.0 31.0 36.0 7.5 37.5 82-83 30.325401572267268 34.0 31.0 35.0 7.5 37.0 84-85 29.999536589175257 34.0 30.5 35.0 4.5 36.5 86-87 29.64578735042818 34.0 30.0 35.0 2.0 36.0 88-89 29.295311650590055 34.0 30.0 35.0 2.0 36.0 90-91 28.881239398455822 34.0 29.0 35.0 2.0 35.5 92-93 28.48397857716229 34.0 29.0 35.0 2.0 35.0 94-95 28.044743813420087 34.0 29.0 35.0 2.0 35.0 96-97 27.471936554790233 33.0 27.0 35.0 2.0 35.0 98-99 26.788396810425926 33.0 26.5 35.0 2.0 35.0 100-101 23.678078700536723 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2647878853014731 1101 2 0.19770842137236144 1101 3 0.16310562426050979 1101 4 0.12893721655019164 1101 5 0.20105836119449805 1101 6 0.18583806783157542 1101 7 0.155290524040268 1101 8 0.149649917258742 1101 9 0.20800374839124203 1101 10-11 0.21159484790217675 1101 12-13 0.23166552278867414 1101 14-15 0.26045491584152103 1101 16-17 0.23043690929630856 1101 18-19 0.28186704205177904 1101 20-21 0.30003812578667777 1101 22-23 0.27152755079664814 1101 24-25 0.2933246559303839 1101 26-27 0.27391213910565426 1101 28-29 0.2636841585705483 1101 30-31 0.10438071085955158 1101 32-33 0.20859674743926604 1101 34-35 0.3505525896226942 1101 36-37 0.3590455743198788 1101 38-39 0.23193189946200476 1101 40-41 0.3605628578273823 1101 42-43 0.24306606523554564 1101 44-45 0.27078260404532273 1101 46-47 0.34800354255920496 1101 48-49 0.27043115062013356 1101 50-51 0.29052437834943134 1101 52-53 0.2505528183395356 1101 54-55 0.2579359800397967 1101 56-57 0.3905605799822993 1101 58-59 0.4208465136203756 1101 60-61 0.3438844510612995 1101 62-63 0.27752176119289373 1101 64-65 0.3341479461235721 1101 66-67 0.1943886601107394 1101 68-69 0.19040295035246402 1101 70-71 0.19154414901504424 1101 72-73 0.24622757160867081 1101 74-75 0.2728402423889058 1101 76-77 0.09613075539934002 1101 78-79 0.2849201619196009 1101 80-81 0.5261953700898694 1101 82-83 0.29203340419731916 1101 84-85 0.14302303324380006 1101 86-87 0.06562868950879874 1101 88-89 0.14859727235440445 1101 90-91 -0.017593649371359987 1101 92-93 0.08296171738131264 1101 94-95 0.21134630456008097 1101 96-97 0.22010001513702804 1101 98-99 0.2926134296112224 1101 100-101 0.565420501586491 1102 1 0.2588263268954023 1102 2 0.2611704915293913 1102 3 0.13486810943705763 1102 4 0.12361451146553293 1102 5 0.12934565261730313 1102 6 0.16576307372877608 1102 7 0.09973775997862333 1102 8 0.1478741971007551 1102 9 0.2534670358596429 1102 10-11 0.2077764555105972 1102 12-13 0.17977533625390407 1102 14-15 0.17385897930507355 1102 16-17 0.2448737319997818 1102 18-19 0.20508608062332456 1102 20-21 0.08904833877657126 1102 22-23 0.13497160137917774 1102 24-25 0.1530193726554927 1102 26-27 0.21044883134047865 1102 28-29 0.2994700929560352 1102 30-31 0.3449437631576586 1102 32-33 0.18306600537685114 1102 34-35 0.25987997187603895 1102 36-37 0.29632470767735697 1102 38-39 0.3067349155625365 1102 40-41 0.359381313336975 1102 42-43 0.326642361673386 1102 44-45 0.2940215433557398 1102 46-47 0.40333811630592464 1102 48-49 0.4994879125273499 1102 50-51 0.48916753904219945 1102 52-53 0.37537005455759953 1102 54-55 0.4341232360917715 1102 56-57 0.47026613320427657 1102 58-59 0.5016850970937057 1102 60-61 0.37384063230752673 1102 62-63 0.3993969553426666 1102 64-65 0.3630491901920294 1102 66-67 0.38822321813983507 1102 68-69 0.28457601895976836 1102 70-71 0.23432504460274828 1102 72-73 0.36892961175617955 1102 74-75 0.3064778128057135 1102 76-77 0.33090291545375194 1102 78-79 0.3722853956753127 1102 80-81 0.41145758583912695 1102 82-83 0.35343572312844174 1102 84-85 0.2033071732212406 1102 86-87 0.1388723193540642 1102 88-89 0.2266506187106785 1102 90-91 0.19745413096606867 1102 92-93 0.16543679009697954 1102 94-95 0.4004367393095478 1102 96-97 0.5341907283483245 1102 98-99 0.6938290330776127 1102 100-101 0.8931434684500275 1103 1 0.18332048694288972 1103 2 0.09349791241523064 1103 3 0.11924294203794261 1103 4 0.07394063172719711 1103 5 0.11453150045499427 1103 6 0.13455961068300581 1103 7 0.12085442189562201 1103 8 0.0995968277470567 1103 9 0.19929105733815078 1103 10-11 0.08982596708015222 1103 12-13 0.14765974890531197 1103 14-15 0.23255393044060924 1103 16-17 0.11838120148225784 1103 18-19 0.23154451528891684 1103 20-21 0.2297890785253145 1103 22-23 0.18067838990883445 1103 24-25 0.17241276111357706 1103 26-27 0.05951522638311246 1103 28-29 0.12139881468031177 1103 30-31 0.13678845320413302 1103 32-33 0.14561293069702685 1103 34-35 0.1752981536163034 1103 36-37 0.2806475795926602 1103 38-39 0.15667352695874825 1103 40-41 0.32390609269155135 1103 42-43 0.21034872273265393 1103 44-45 0.409933512312179 1103 46-47 0.542718369959772 1103 48-49 0.4365296407076116 1103 50-51 0.44731026058221346 1103 52-53 0.46186357072111406 1103 54-55 0.4285242043818229 1103 56-57 0.4261209775472068 1103 58-59 0.44313772501876514 1103 60-61 0.46853349108802433 1103 62-63 0.4848619713379918 1103 64-65 0.4611998451109329 1103 66-67 0.393340408234522 1103 68-69 0.3915519552698967 1103 70-71 0.47291257639518847 1103 72-73 0.4939147467146867 1103 74-75 0.37599920132378983 1103 76-77 0.44835107261715024 1103 78-79 0.7228747209149198 1103 80-81 0.7691856128972496 1103 82-83 0.6486701783126172 1103 84-85 0.6288682682739406 1103 86-87 0.5471649233665019 1103 88-89 0.3730244056697245 1103 90-91 0.37286188842858436 1103 92-93 0.31559232396356407 1103 94-95 0.4851992146951787 1103 96-97 0.46643761017151064 1103 98-99 0.6471747655518669 1103 100-101 0.9514242072131296 1104 1 0.15493990301010285 1104 2 0.12952795533368544 1104 3 0.1224835876282242 1104 4 0.09947832138445278 1104 5 0.03884450293337949 1104 6 0.06499816475421483 1104 7 0.08213683741880118 1104 8 0.11372485772952956 1104 9 0.15042779190866895 1104 10-11 0.16109689738299693 1104 12-13 0.13659419247660765 1104 14-15 0.1711469909495804 1104 16-17 0.0653223548401698 1104 18-19 0.13334678815357393 1104 20-21 0.14956587233538698 1104 22-23 0.046206861165558166 1104 24-25 0.14830981727025971 1104 26-27 0.2651268143659493 1104 28-29 0.22921224029840204 1104 30-31 0.3103431288977774 1104 32-33 0.3182782574213192 1104 34-35 0.13761998157979605 1104 36-37 0.22023532063116846 1104 38-39 0.5199788524740754 1104 40-41 0.35997028902837513 1104 42-43 0.43717487276590106 1104 44-45 0.31832860389170037 1104 46-47 0.39625732903611777 1104 48-49 0.4636210086578032 1104 50-51 0.3515507248269074 1104 52-53 0.2967564257051052 1104 54-55 0.26029187046415103 1104 56-57 0.19930721946221297 1104 58-59 0.43571827268242913 1104 60-61 0.6293790148745231 1104 62-63 0.434788375255053 1104 64-65 0.4727066757946119 1104 66-67 0.3698272909039275 1104 68-69 0.4304486185111429 1104 70-71 0.5412428369283475 1104 72-73 0.63233900470901 1104 74-75 0.7403253513570398 1104 76-77 0.7327573169500994 1104 78-79 0.7582481416971234 1104 80-81 0.7500027258755502 1104 82-83 0.5318845287931815 1104 84-85 0.44991599238685964 1104 86-87 0.48945531809539133 1104 88-89 0.3685766029737181 1104 90-91 0.3432212549280109 1104 92-93 0.40158594664385205 1104 94-95 0.46390365161714087 1104 96-97 0.844805651034715 1104 98-99 1.0222566733074103 1104 100-101 1.0623960684233076 1105 1 0.11554211478737386 1105 2 0.07264110869382279 1105 3 0.008248446742705084 1105 4 0.08517991752071907 1105 5 0.14662358408311604 1105 6 0.08458185876202862 1105 7 0.09427782810733731 1105 8 0.0601419241718375 1105 9 0.06758273766592282 1105 10-11 0.032473069406528055 1105 12-13 0.11656959919608312 1105 14-15 0.14518214963286624 1105 16-17 0.1752994604375715 1105 18-19 0.2651005964716049 1105 20-21 0.2192938091694856 1105 22-23 0.0863731894049522 1105 24-25 0.0984576196503042 1105 26-27 0.2571199217754767 1105 28-29 0.12802593810846474 1105 30-31 0.17892699279338586 1105 32-33 0.10360334984837039 1105 34-35 0.18921830211760238 1105 36-37 0.1844804120874315 1105 38-39 0.2913477435921763 1105 40-41 0.2597508910783475 1105 42-43 0.2154266464753647 1105 44-45 0.31665674620913364 1105 46-47 0.3725584672843354 1105 48-49 0.3067399867422722 1105 50-51 0.12586126353300386 1105 52-53 0.15739876336113667 1105 54-55 0.14118240609609245 1105 56-57 -0.06260830585511457 1105 58-59 0.1325816586270534 1105 60-61 0.2639474594304261 1105 62-63 0.15727148759942366 1105 64-65 0.13452609316038178 1105 66-67 0.04468697167666846 1105 68-69 0.2714938329338139 1105 70-71 0.2612281133196035 1105 72-73 0.09299558058008728 1105 74-75 0.342701191694875 1105 76-77 0.1752159978118364 1105 78-79 0.31310377752241436 1105 80-81 0.5135072683515425 1105 82-83 0.37724392237466375 1105 84-85 0.11544284679301953 1105 86-87 -0.021754202092942876 1105 88-89 0.06281222925293406 1105 90-91 0.26268182073526347 1105 92-93 0.3524919339750774 1105 94-95 0.26699933080426774 1105 96-97 0.47324438221559717 1105 98-99 0.42263803095357844 1105 100-101 0.583367179408576 1106 1 0.16667699221198262 1106 2 0.10384720415997606 1106 3 0.10541785992869279 1106 4 0.10994780495253309 1106 5 0.01508863438878194 1106 6 0.11056154503589966 1106 7 0.05023542896810085 1106 8 0.07959340233046674 1106 9 0.08655455247204458 1106 10-11 0.13176591146750383 1106 12-13 0.024716258204314556 1106 14-15 0.13355309423596395 1106 16-17 0.1262495848871268 1106 18-19 0.1463045346324492 1106 20-21 0.15159638876730952 1106 22-23 0.15389465459278995 1106 24-25 0.1128520707913907 1106 26-27 0.12113620403730607 1106 28-29 0.2020056675284465 1106 30-31 0.06931026504800997 1106 32-33 0.1492524358250762 1106 34-35 0.04646974683800664 1106 36-37 0.1405404849504137 1106 38-39 0.23448589472759096 1106 40-41 0.4279984580424596 1106 42-43 0.2627147788691886 1106 44-45 -0.008690175451022242 1106 46-47 0.13442634312063007 1106 48-49 0.3643956565451205 1106 50-51 0.2504357013527283 1106 52-53 0.34607567453139154 1106 54-55 0.4707613540322271 1106 56-57 0.3921123837814591 1106 58-59 0.5112581787857167 1106 60-61 0.4524189209778058 1106 62-63 0.28369682595928225 1106 64-65 0.16416207485564627 1106 66-67 0.10317236132645746 1106 68-69 0.15472091898062956 1106 70-71 0.22252217965134946 1106 72-73 0.235085483582246 1106 74-75 0.1654544593382603 1106 76-77 0.1776751573256874 1106 78-79 0.2548091745609753 1106 80-81 0.4872914582699188 1106 82-83 0.5125652799668927 1106 84-85 0.3754793726685506 1106 86-87 0.46382151527848947 1106 88-89 0.07705078137747279 1106 90-91 0.16813909530359794 1106 92-93 0.2711047259865751 1106 94-95 0.24702571734484025 1106 96-97 0.3748291252131217 1106 98-99 0.5061533869224348 1106 100-101 0.5360697773434957 1107 1 0.13853100530767648 1107 2 0.047866097284064324 1107 3 0.04551569835373925 1107 4 0.10223573139558795 1107 5 0.10131729699724445 1107 6 0.10556024467749126 1107 7 0.10643630996091957 1107 8 0.10092673024563936 1107 9 0.04008197784366274 1107 10-11 0.017089030214613388 1107 12-13 0.11737830859861731 1107 14-15 0.1280968377511087 1107 16-17 0.1047430451679432 1107 18-19 0.16213233466983468 1107 20-21 0.23372198036265956 1107 22-23 0.13822753686429223 1107 24-25 0.14041306572811862 1107 26-27 0.11439398112878507 1107 28-29 0.1499219109013623 1107 30-31 0.02251497126183466 1107 32-33 0.03304594026390362 1107 34-35 0.03704587334120646 1107 36-37 0.018146619933034458 1107 38-39 -0.12480516612105674 1107 40-41 0.05580133996178205 1107 42-43 -0.12695522809314497 1107 44-45 -0.036755228589626654 1107 46-47 0.0676549705110574 1107 48-49 0.04412652299019015 1107 50-51 0.025070551229056548 1107 52-53 -0.07083311616237609 1107 54-55 -0.043915764265172186 1107 56-57 -0.06675277582398564 1107 58-59 -0.0738699200631956 1107 60-61 -0.08095135743434412 1107 62-63 0.040647140077112454 1107 64-65 0.006167173344245214 1107 66-67 -0.0164275955991684 1107 68-69 0.043528761225481105 1107 70-71 -0.02900658806942147 1107 72-73 0.005699212116176966 1107 74-75 -0.07054450714770866 1107 76-77 -0.11491479767649437 1107 78-79 0.23421898115974926 1107 80-81 0.47789687670087133 1107 82-83 0.29157309295367995 1107 84-85 0.23706942655215357 1107 86-87 0.23349553167926018 1107 88-89 0.085980925039987 1107 90-91 0.07511758385367173 1107 92-93 0.08708792562680756 1107 94-95 0.20674948600024123 1107 96-97 0.09760930504185339 1107 98-99 0.006705361977225266 1107 100-101 0.2413691103679909 1108 1 0.1793821170419605 1108 2 0.1433830955206048 1108 3 0.04445903846407617 1108 4 0.10541750790545024 1108 5 0.05806064088257301 1108 6 0.12950816870313986 1108 7 0.10881118255504418 1108 8 0.16204919628422232 1108 9 0.15168717061078496 1108 10-11 0.21346345655108934 1108 12-13 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-0.7844113062343965 2316 52-53 -0.8974670033454473 2316 54-55 -0.7289185202251574 2316 56-57 -0.8175637276843446 2316 58-59 -0.6986408476320278 2316 60-61 -0.5371947807116655 2316 62-63 -0.5735518682815339 2316 64-65 -0.40321689402407657 2316 66-67 -0.47278715052344467 2316 68-69 -0.7191729997840994 2316 70-71 -0.5091906212758239 2316 72-73 -0.6099935927325504 2316 74-75 -0.6467218252031408 2316 76-77 -0.44591315481550353 2316 78-79 -0.36025356213592374 2316 80-81 -0.3967772219710035 2316 82-83 -0.486349792481267 2316 84-85 -0.7588128635450175 2316 86-87 -0.8407261420472238 2316 88-89 -0.783898685677908 2316 90-91 -0.9766413615133978 2316 92-93 -0.8794808143810648 2316 94-95 -0.8280874869125618 2316 96-97 -0.983145364726699 2316 98-99 -1.412423521460319 2316 100-101 -2.111578285620123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 7.0 5 34.0 6 81.0 7 165.0 8 469.0 9 924.0 10 1566.0 11 2653.0 12 4174.0 13 6115.0 14 7893.0 15 10928.0 16 15235.0 17 22326.0 18 19996.0 19 10596.0 20 8458.0 21 8416.0 22 8363.0 23 8775.0 24 10177.0 25 11917.0 26 14380.0 27 18079.0 28 22388.0 29 28055.0 30 34621.0 31 44078.0 32 56713.0 33 75026.0 34 104107.0 35 151742.0 36 235355.0 37 374401.0 38 307481.0 39 26184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30412893646551 20.855652536184056 15.464467506106875 21.37575102124356 2 15.18314559260322 22.055751913429788 40.19255987300711 22.56854262095988 3 17.94209388100076 27.69617874345829 30.829359668900313 23.532367706640642 4 12.296701580379228 18.48104162403852 38.579753275355024 30.642503520227233 5 12.543611593918625 38.81748180920537 34.16624439928151 14.472662197594499 6 29.106800606823008 38.894000903212216 17.764101794195952 14.235096695768826 7 26.88872449727039 34.63425353626954 21.707482737871107 16.769539228588968 8 26.25939762004648 34.62198548200506 20.182809778670496 18.93580711927796 9 27.24922240208441 16.619528513537894 21.13238112649693 34.99886795788077 10-11 23.943538339905636 28.035567915868082 27.643076200358134 20.377817543868147 12-13 25.536865408847675 25.750954745213107 28.351506056279497 20.360673789659725 14-15 22.595015866750774 26.50243782546211 27.455593075050157 23.446953232736963 16-17 20.662226679092903 30.001244035967474 27.90769192584335 21.428837359096278 18-19 20.435595156582874 27.416850253267782 31.90178162245151 20.24577296769784 20-21 21.392432458610987 26.733783029105112 32.052583228274834 19.821201284009064 22-23 21.195036933630853 26.760900597016008 31.832600633701443 20.211461835651697 24-25 20.985233096945034 27.034323726451476 32.05157155639719 19.928871620206294 26-27 20.73602193990138 27.222096096041554 31.921436506567137 20.12044545748993 28-29 20.38965850847039 26.815857567167996 32.489311362345724 20.305172562015894 30-31 20.709089384187628 26.889558164228944 32.201869779150236 20.199482672433184 32-33 20.670482544025862 27.148867646969798 31.989442268459385 20.19120754054496 34-35 20.8715843487863 26.943809450008715 31.931828638679555 20.252777562525427 36-37 21.211127700823308 26.571300040081713 31.516517661988853 20.701054597106125 38-39 20.642825163893807 27.232117586410336 31.67793296283863 20.44712428685723 40-41 20.754172094094724 27.072682179197 31.95632306594616 20.21682266076211 42-43 20.930695059350874 27.176960467663204 31.156014667750746 20.73632980523518 44-45 20.740495445496244 27.293181214855196 31.087405366120475 20.87891797352809 46-47 21.29949780655488 27.59218659523983 30.294272464234872 20.814043133970415 48-49 21.353515094701137 27.899370071637662 29.803321055214006 20.943793778447194 50-51 21.554120114902517 27.88647171176298 29.283887313200168 21.275520860134336 52-53 21.493635413453703 28.291104047588274 29.307306932197548 20.90795360676048 54-55 21.38363275942282 28.16688757998964 29.21446773913804 21.235011921449498 56-57 21.38633790794675 28.656643682614245 28.845732412356906 21.111285997082092 58-59 21.357560869004587 27.97077488684016 29.37336964779852 21.29829459635673 60-61 21.465032005918097 28.31776119601763 29.367079488728614 20.850127309335655 62-63 21.284705133117942 28.3022203173231 29.625166703896948 20.78790784566201 64-65 21.23229617826297 27.92632958016954 29.51623781150069 21.325136430066802 66-67 21.42093721197372 28.554862905166424 28.90053227634783 21.123667606512022 68-69 21.267184727095074 28.135203419888953 29.389481534978767 21.208130318037202 70-71 21.36706137258861 28.24308089165863 29.378979132417836 21.010878603334927 72-73 21.391868983471575 28.334897378458557 28.9547435975056 21.318490040564267 74-75 21.308796749227504 28.754891657060938 28.570213440917502 21.366098152794056 76-77 21.309503413513724 28.69839697738914 28.92784128023867 21.06425832885846 78-79 21.269453602414696 28.523504459143613 28.683731519626377 21.523310418815313 80-81 21.472964583675747 29.22861504123031 28.136555965492636 21.161864409601307 82-83 21.611209572572992 29.022325610961357 28.132903298063784 21.233561518401867 84-85 21.184663800621053 28.80260819634965 28.110604378856802 21.90212362417249 86-87 21.16519218685112 28.725689849517096 28.51901649149273 21.59010147213905 88-89 21.361086752995796 28.63541718060861 28.455982707941036 21.547513358454562 90-91 21.22151097184756 28.820471903255957 28.512940532634644 21.445076592261834 92-93 21.211197561562525 28.585170510525142 28.331792157711455 21.871839770200875 94-95 21.032745020112063 28.203438588823147 28.590696783250735 22.17311960781406 96-97 21.431217764002227 28.29146184952902 28.29327796207957 21.98404242438918 98-99 20.998783809225735 28.508171603368044 28.16722653414687 22.32581805325935 100-101 20.702003757556202 28.920439634681028 28.213332574597967 22.164224033164807 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 990.0 1 726.5 2 522.5 3 614.5 4 567.5 5 571.5 6 950.0 7 2086.5 8 3519.0 9 4102.0 10 3671.0 11 3197.0 12 3239.0 13 3425.5 14 3714.0 15 3956.5 16 4121.0 17 4285.5 18 4431.0 19 4583.5 20 4863.5 21 5383.5 22 5940.5 23 6916.0 24 7986.5 25 9352.5 26 11905.5 27 14673.0 28 16377.0 29 18803.0 30 23038.5 31 26903.5 32 31931.5 33 37962.5 34 44573.0 35 49788.0 36 57287.0 37 64670.5 38 64687.5 39 62715.0 40 60078.0 41 57108.0 42 54584.5 43 55367.5 44 56842.0 45 56935.0 46 53866.0 47 49413.0 48 50456.0 49 50217.5 50 47050.0 51 44390.5 52 42856.0 53 42500.0 54 41441.5 55 38702.0 56 34829.0 57 31188.5 58 29053.0 59 26852.0 60 23515.5 61 19804.0 62 16205.0 63 13027.0 64 9850.5 65 7435.0 66 5455.0 67 4413.5 68 3227.5 69 1799.5 70 1262.0 71 944.0 72 795.0 73 626.5 74 452.5 75 339.5 76 207.0 77 106.5 78 62.5 79 38.5 80 21.5 81 11.0 82 7.5 83 3.5 84 2.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02808917343974933 2 0.008233033594409286 3 0.010533440039905998 4 0.0 5 0.005145645996505804 6 0.0 7 0.0 8 6.053701172359769E-5 9 0.0 10-11 0.0 12-13 9.080551758539653E-5 14-15 0.0 16-17 3.0268505861798846E-5 18-19 0.004419201855822631 20-21 0.0014831567872281434 22-23 0.0 24-25 0.0048429609378878155 26-27 0.005872090137188976 28-29 0.007748737500620505 30-31 0.030904144484896624 32-33 7.264441406831723E-4 34-35 0.004479738867546229 36-37 0.01549747500124101 38-39 0.007657931983035107 40-41 0.009897801416808223 42-43 0.00575101611374178 44-45 0.0033598041506596715 46-47 0.0017253048341225343 48-49 0.0016647678223989364 50-51 0.0028452395510090915 52-53 0.0028149710451472925 54-55 0.0016647678223989364 56-57 7.264441406831723E-4 58-59 9.080551758539652E-4 60-61 0.0 62-63 6.053701172359769E-5 64-65 0.007264441406831722 66-67 8.777866699921665E-4 68-69 9.383236817157642E-4 70-71 0.003723026221001258 72-73 0.01101773613369478 74-75 0.0054483310551237925 76-77 0.00393490576203385 78-79 0.009897801416808223 80-81 1.5134252930899423E-4 82-83 0.004812692432026016 84-85 7.567126465449712E-4 86-87 0.0 88-89 0.001543693798951741 90-91 0.0010896662110247584 92-93 2.724165527561896E-4 94-95 7.567126465449712E-4 96-97 1.2107402344719538E-4 98-99 1.8161103517079307E-4 100-101 6.356386230977757E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1651882.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.93183597271882 #Duplication Level Percentage of deduplicated Percentage of total 1 74.50840076887022 31.24274039629863 2 13.373080414411112 11.215156287741308 3 4.6382014518203185 5.834649074584677 4 2.2016959287446696 3.692846101836972 5 1.2078981061951688 2.532469263036676 6 0.7484092372402398 1.8829304025855205 7 0.5100374699529091 1.4970765271004123 8 0.37092601823623284 1.2442887163756342 9 0.2728917398232608 1.029856650532095 >10 1.7558100705294524 14.427359638842793 >50 0.23317359648265387 6.881944939956624 >100 0.16848403098217737 13.156648741995294 >500 0.008677236877583863 2.4267747137354974 >1k 0.0018800679901431704 1.0398903691123074 >5k 1.4462061462639772E-4 0.43334829587570256 >10k+ 2.8924122925279544E-4 1.4620198803900522 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13907 0.8418882220400732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10202 0.6175985936041436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7146 0.4325974857768291 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2460 0.1489210488400503 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1944 0.11768395079067391 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.080551758539653E-5 0.0 22-23 0.0 0.0 0.0 3.0268505861798847E-4 0.0 24-25 0.0 0.0 0.0 6.961756348213735E-4 0.0 26-27 0.0 0.0 0.0 0.0012712772461955515 0.0 28-29 0.0 0.0 0.0 0.002633360009976499 0.0 30-31 0.0 0.0 0.0 0.004964034961335011 0.0 32-33 0.0 0.0 0.0 0.007113098877522728 0.0 34-35 0.0 0.0 0.0 0.009837264405084624 6.053701172359769E-5 36-37 0.0 0.0 0.0 0.013590559131947682 6.053701172359769E-5 38-39 0.0 0.0 0.0 0.01994694536292544 6.053701172359769E-5 40-41 0.0 0.0 0.0 0.026696822170106584 6.053701172359769E-5 42-43 0.0 0.0 0.0 0.032871597365913545 6.053701172359769E-5 44-45 0.0 0.0 0.0 0.03614059599898782 6.053701172359769E-5 46-47 0.0 0.0 0.0 0.04089275141929024 6.053701172359769E-5 48-49 0.0 0.0 0.0 0.04752155420302419 6.053701172359769E-5 50-51 0.0 0.0 0.0 0.054695190092270515 6.053701172359769E-5 52-53 0.0 0.0 0.0 0.07119152578695088 6.053701172359769E-5 54-55 0.0 0.0 0.0 0.08223953042650746 6.053701172359769E-5 56-57 0.0 0.0 0.0 0.10960225972557361 6.053701172359769E-5 58-59 0.0 0.0 0.0 0.16108898819649348 6.053701172359769E-5 60-61 0.0 0.0 0.0 0.19965106466442517 6.053701172359769E-5 62-63 0.0 0.0 0.0 0.21817539025184607 6.053701172359769E-5 64-65 0.0 0.0 0.0 0.2602183448938847 6.053701172359769E-5 66-67 6.053701172359769E-5 0.0 0.0 0.3530821208778835 6.053701172359769E-5 68-69 6.053701172359769E-5 0.0 0.0 0.48820073104495354 6.053701172359769E-5 70-71 6.053701172359769E-5 0.0 0.0 0.5952907047839979 1.2107402344719538E-4 72-73 6.053701172359769E-5 0.0 0.0 0.7245977618256025 1.8161103517079307E-4 74-75 6.053701172359769E-5 0.0 0.0 0.884203593234868 1.8161103517079307E-4 76-77 6.053701172359769E-5 0.0 0.0 0.9797915347464285 1.8161103517079307E-4 78-79 6.053701172359769E-5 0.0 0.0 1.097838707607444 1.8161103517079307E-4 80-81 6.053701172359769E-5 0.0 0.0 1.2713680517131367 1.8161103517079307E-4 82-83 6.053701172359769E-5 0.0 0.0 1.4240121267741883 1.8161103517079307E-4 84-85 6.053701172359769E-5 0.0 0.0 1.636829991488496 1.8161103517079307E-4 86-87 6.053701172359769E-5 0.0 0.0 1.9663026777941766 1.8161103517079307E-4 88-89 6.053701172359769E-5 0.0 0.0 2.384219938228033 1.8161103517079307E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5775 0.0 53.73073 1 GTATCAA 10335 0.0 41.058445 1 ATCAACG 13630 0.0 31.08562 3 TCAACGC 13765 0.0 30.88147 4 TATCAAC 13895 0.0 30.493692 2 CAACGCA 14025 0.0 30.275114 5 AACGCAG 14415 0.0 29.487179 6 ACGCAGA 16410 0.0 25.757652 7 CGCAGAG 16410 0.0 25.69977 8 GTACATG 9635 0.0 25.053795 1 GCAGAGT 17165 0.0 24.26502 9 ACATGGG 9615 0.0 23.366972 3 TACATGG 10160 0.0 23.283024 2 GAGTACT 10360 0.0 23.220459 12-13 CATGGGG 2980 0.0 22.472633 4 GTACTTT 10910 0.0 21.942686 14-15 AGTACTT 11020 0.0 21.398767 12-13 CAGAGTA 16950 0.0 20.860266 10-11 ATGGGAT 2715 0.0 20.642548 5 GTATAGG 875 0.0 20.636549 1 >>END_MODULE