FastQCFastQC Report
Thu 26 May 2016
SRR937009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706324
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121000.709126754356148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84040.49252076393463373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58550.34313530138473114No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.11498402413609608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49700.051.056891
GTATCAA96400.044.2658421
ATCAACG123400.034.2338753
TCAACGC124200.034.0133674
TATCAAC126300.033.523072
CAACGCA126850.033.377725
AACGCAG130050.032.6641356
ACGCAGA146600.028.7164697
CGCAGAG146850.028.6028678
GCAGAGT151800.026.8884269
GTACATG96050.026.7156581
ACATGGG96800.025.3305033
TACATGG100900.025.2902162
CATGGGG28750.024.6273614
GAGTACT86550.023.96269612-13
AGTACTT93800.022.36384212-13
GTACTTT92200.022.28617514-15
AGAGTAC142750.022.01900710-11
TACGCCG1100.002871177621.5996535
CATGGGA66500.021.3657934
Produced by FastQC (version 0.11.5)