##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937009_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1706324 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.808008912727008 33.0 31.0 34.0 30.0 34.0 2 31.930835527133183 33.0 31.0 34.0 30.0 34.0 3 31.92928130882529 33.0 31.0 34.0 30.0 34.0 4 35.52172858144174 37.0 35.0 37.0 33.0 37.0 5 35.51833121962769 37.0 35.0 37.0 33.0 37.0 6 35.62740956582689 37.0 35.0 37.0 33.0 37.0 7 35.57249736861229 37.0 35.0 37.0 33.0 37.0 8 35.532770446878786 37.0 35.0 37.0 33.0 37.0 9 37.13339084488057 39.0 37.0 39.0 33.0 39.0 10-11 37.00977012572056 39.0 37.0 39.0 33.0 39.0 12-13 36.9198792257508 39.0 37.0 39.0 33.0 39.0 14-15 38.07006172333038 40.0 38.0 41.0 33.0 41.0 16-17 38.006960870268486 40.0 38.0 41.0 33.0 41.0 18-19 37.99507684355375 40.0 38.0 41.0 33.0 41.0 20-21 37.90867297183888 40.0 38.0 41.0 33.0 41.0 22-23 37.774501208445756 40.0 37.5 41.0 32.5 41.0 24-25 37.89151972310066 40.0 38.0 41.0 33.0 41.0 26-27 37.842052564460204 40.0 38.0 41.0 33.0 41.0 28-29 37.74507537841582 40.0 37.0 41.0 32.5 41.0 30-31 37.53236255248124 40.0 37.0 41.0 32.0 41.0 32-33 37.30671812621753 40.0 36.5 41.0 31.5 41.0 34-35 37.113537053924105 39.5 36.0 41.0 31.0 41.0 36-37 36.91158742419376 39.0 36.0 41.0 31.0 41.0 38-39 36.74328146354385 39.0 35.0 41.0 30.0 41.0 40-41 36.5435359873037 39.0 35.0 41.0 30.0 41.0 42-43 36.28868784591906 39.0 35.0 41.0 30.0 41.0 44-45 35.96996760287026 39.0 35.0 40.5 29.5 41.0 46-47 35.540091155020974 39.0 35.0 40.0 27.5 41.0 48-49 35.20832913327129 38.5 35.0 40.0 26.0 41.0 50-51 34.47898406164363 38.0 34.0 39.5 25.0 40.5 52-53 34.664016329841225 38.0 34.5 39.5 25.5 40.5 54-55 35.07101464903501 39.0 35.0 40.0 26.0 41.0 56-57 34.98366547033271 39.0 35.0 41.0 25.5 41.0 58-59 34.747326416319524 38.0 34.5 40.0 25.0 41.0 60-61 34.498191140721225 38.0 34.0 40.0 24.5 41.0 62-63 34.16502580986963 38.0 34.0 40.0 23.0 41.0 64-65 33.84266176880827 37.0 34.0 40.0 22.5 41.0 66-67 33.47632864567339 37.0 33.0 39.5 22.0 41.0 68-69 33.08569240073983 36.0 33.0 39.0 20.5 41.0 70-71 32.70117398571432 36.0 32.5 39.0 19.5 40.5 72-73 32.310236508423955 35.0 32.0 38.5 18.5 40.0 74-75 31.864405880711985 35.0 32.0 37.5 17.0 39.5 76-77 31.440953476596473 35.0 31.0 37.0 12.5 39.0 78-79 31.009913709236933 35.0 31.0 36.5 8.0 39.0 80-81 30.57664282984943 35.0 31.0 36.0 7.0 38.5 82-83 30.162738143517878 34.5 30.5 35.5 3.5 37.0 84-85 29.767954386154095 34.0 30.0 35.0 2.0 37.0 86-87 29.421294548983663 34.0 30.0 35.0 2.0 36.0 88-89 29.070492180851936 34.0 29.0 35.0 2.0 36.0 90-91 28.696905452891713 34.0 29.0 35.0 2.0 36.0 92-93 28.34693586915498 34.0 29.0 35.0 2.0 35.0 94-95 27.94981668194317 34.0 29.0 35.0 2.0 35.0 96-97 27.457216507533154 34.0 27.0 35.0 2.0 35.0 98-99 26.828518792445045 34.0 26.0 35.0 2.0 35.0 100-101 24.26125050107717 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26760782843830455 1101 2 0.34187905678960107 1101 3 0.28387867801379585 1101 4 0.16245584183113948 1101 5 0.2720135343043779 1101 6 0.22100638121131766 1101 7 0.1148733033485243 1101 8 0.09059244709717973 1101 9 0.17013925339259828 1101 10-11 0.2908039360269683 1101 12-13 0.30962499766337714 1101 14-15 0.3525813875932613 1101 16-17 0.4197145395458364 1101 18-19 0.4028258894085539 1101 20-21 0.47623969379696973 1101 22-23 0.5248895722686626 1101 24-25 0.4603904568417221 1101 26-27 0.4589473795106116 1101 28-29 0.390901347678998 1101 30-31 0.3289280747974672 1101 32-33 0.42931879977117404 1101 34-35 0.5220284818748269 1101 36-37 0.3503007871146053 1101 38-39 0.5265128874466001 1101 40-41 0.5147289133180948 1101 42-43 0.6286617891436492 1101 44-45 0.5356060130980183 1101 46-47 0.40280595049863166 1101 48-49 0.34763473456691685 1101 50-51 0.43977175586055495 1101 52-53 0.46332389623652404 1101 54-55 0.47555418301175933 1101 56-57 0.4045302676390108 1101 58-59 0.40456648767657555 1101 60-61 0.6827449602961622 1101 62-63 0.6955210699988612 1101 64-65 0.4664956641510756 1101 66-67 0.4819332619074572 1101 68-69 0.5871889665248204 1101 70-71 0.5167330780956405 1101 72-73 0.33465002247467623 1101 74-75 0.38573126815098746 1101 76-77 0.4477928348788325 1101 78-79 0.3966210820557876 1101 80-81 0.5052527203303434 1101 82-83 0.4924809306694087 1101 84-85 0.5047690799063247 1101 86-87 0.39617761448633004 1101 88-89 0.4054148027149189 1101 90-91 0.39736043755171835 1101 92-93 0.5320088878930562 1101 94-95 0.5477436326810405 1101 96-97 0.362234526861716 1101 98-99 0.21660439942717602 1101 100-101 0.5354637806121865 1102 1 0.10292383396235039 1102 2 0.23448409741708076 1102 3 0.11183021011434491 1102 4 0.0609749561704831 1102 5 0.1265647458139867 1102 6 0.14823464231232464 1102 7 0.16863428499107158 1102 8 0.10623221490296686 1102 9 0.19495762151506568 1102 10-11 0.12934639203214005 1102 12-13 0.06370722154947828 1102 14-15 0.17346641698827625 1102 16-17 0.4141008163096558 1102 18-19 0.405200637122924 1102 20-21 0.25325539243246453 1102 22-23 0.23496907371192322 1102 24-25 0.034324269015712616 1102 26-27 0.21411276891537767 1102 28-29 0.08680500807204083 1102 30-31 0.03732283301783923 1102 32-33 0.31460808938342666 1102 34-35 0.17812568420658437 1102 36-37 0.15273755883045936 1102 38-39 0.233563583550783 1102 40-41 0.2150694441243033 1102 42-43 0.2720155140782339 1102 44-45 0.2263546172448656 1102 46-47 0.2997826060772937 1102 48-49 0.356136982686472 1102 50-51 0.20953282567938913 1102 52-53 0.265105107322789 1102 54-55 0.4137156773793649 1102 56-57 0.463454822555434 1102 58-59 0.3971496595830075 1102 60-61 0.21466515173889889 1102 62-63 0.30023379190814126 1102 64-65 0.376367600620064 1102 66-67 0.20116581258440647 1102 68-69 0.47863343688217697 1102 70-71 0.2855401155653041 1102 72-73 0.21055317184605826 1102 74-75 0.2947759256825542 1102 76-77 0.40855964792982746 1102 78-79 0.3907138971162958 1102 80-81 0.34328202541854225 1102 82-83 0.42847596874928584 1102 84-85 0.3294649781809511 1102 86-87 0.359876790063101 1102 88-89 0.3442430049983294 1102 90-91 0.3465725248171836 1102 92-93 0.4476965765045158 1102 94-95 0.50536528276497 1102 96-97 0.38525868290568965 1102 98-99 0.31171980890958295 1102 100-101 0.5507082581338736 1103 1 0.1388635410853034 1103 2 0.2983215069494989 1103 3 0.24067557142605622 1103 4 0.2362182132129078 1103 5 0.28270303379710526 1103 6 0.25322201207921324 1103 7 0.22679598434985593 1103 8 0.2654872606936962 1103 9 0.3256706491911814 1103 10-11 0.40149812784996897 1103 12-13 0.4301990631804955 1103 14-15 0.45576877885115863 1103 16-17 0.4950754844591998 1103 18-19 0.5258109378416194 1103 20-21 0.5272304780072972 1103 22-23 0.5890712391487369 1103 24-25 0.3658838765775556 1103 26-27 0.5340167106785785 1103 28-29 0.585282984708428 1103 30-31 0.6737625216976539 1103 32-33 0.6241634398972735 1103 34-35 0.5226444496361324 1103 36-37 0.619833937367531 1103 38-39 0.6419837124192327 1103 40-41 0.4593405938271289 1103 42-43 0.5173919252768968 1103 44-45 0.6875921810627688 1103 46-47 0.6633747616662689 1103 48-49 0.6761050110406117 1103 50-51 0.4317802137195841 1103 52-53 0.5503914098555498 1103 54-55 0.5802050776164478 1103 56-57 0.6380495179902113 1103 58-59 0.6483721477933386 1103 60-61 0.7355795386876309 1103 62-63 0.7610924089274675 1103 64-65 0.7597115619781007 1103 66-67 0.6659367633932334 1103 68-69 0.6070713242378574 1103 70-71 0.8454180883273068 1103 72-73 0.5315238966136562 1103 74-75 0.7292830122265208 1103 76-77 0.7063907347826799 1103 78-79 0.5611084661824037 1103 80-81 0.6811897879632589 1103 82-83 0.8060459007538121 1103 84-85 0.641436645273533 1103 86-87 0.5927362806768706 1103 88-89 0.5617664284482906 1103 90-91 0.4717039155970504 1103 92-93 0.46559955710153744 1103 94-95 0.4962038193191418 1103 96-97 0.6957466724416612 1103 98-99 0.5766714228700067 1103 100-101 0.6620246763223605 1104 1 0.22434126994538062 1104 2 0.23493402444437095 1104 3 0.27673114056986137 1104 4 0.23150478938183738 1104 5 0.33863311546247843 1104 6 0.17845946672843382 1104 7 0.26092202215236426 1104 8 0.3402030964389411 1104 9 0.26666962327838917 1104 10-11 0.286076144780921 1104 12-13 0.3131184644822511 1104 14-15 0.4718496221378885 1104 16-17 0.3457971054014095 1104 18-19 0.29686465558651776 1104 20-21 0.5868017857403771 1104 22-23 0.44426806493379445 1104 24-25 0.516752505387359 1104 26-27 0.3346928109870291 1104 28-29 0.35187635975768927 1104 30-31 0.22859959859355428 1104 32-33 0.4118733783425057 1104 34-35 0.3483416117303548 1104 36-37 0.3124299553311971 1104 38-39 0.4549291522029151 1104 40-41 0.5291960828002686 1104 42-43 0.6694546214572483 1104 44-45 0.4817786186315587 1104 46-47 0.4209209107565357 1104 48-49 0.47432588462913117 1104 50-51 0.32506362722358517 1104 52-53 0.40027607959316214 1104 54-55 0.3909067578034282 1104 56-57 0.49063782462263816 1104 58-59 0.5047137835467339 1104 60-61 0.4730940611286272 1104 62-63 0.5463853376027501 1104 64-65 0.5058414588432925 1104 66-67 0.522408761409352 1104 68-69 0.48656266512625734 1104 70-71 0.3848078740406251 1104 72-73 0.5794899905356274 1104 74-75 0.38840140663903 1104 76-77 0.3996406872238083 1104 78-79 0.4680221672137499 1104 80-81 0.5684616752351452 1104 82-83 0.3827388267114422 1104 84-85 0.35841465269271566 1104 86-87 0.5829888352676598 1104 88-89 0.6668116603317564 1104 90-91 0.3032686534117879 1104 92-93 0.1168902708922488 1104 94-95 0.338220468321083 1104 96-97 0.25268950328154816 1104 98-99 0.31865435602940906 1104 100-101 0.17229314614965574 1105 1 0.19512136273604241 1105 2 0.15361028391595966 1105 3 0.23000246293183935 1105 4 0.21247723740100355 1105 5 0.32910270180247636 1105 6 0.12107376751848165 1105 7 0.15644637019340024 1105 8 0.34914524963860316 1105 9 0.29166831603292565 1105 10-11 0.34932068049486276 1105 12-13 0.2917836242514156 1105 14-15 0.233084208525419 1105 16-17 0.3342496714054164 1105 18-19 0.230750418970338 1105 20-21 0.12439608567477478 1105 22-23 0.1962693389039245 1105 24-25 0.014286565073817314 1105 26-27 0.07424821680356075 1105 28-29 0.09644683454924063 1105 30-31 -0.06970074461780484 1105 32-33 0.08086634773869861 1105 34-35 -0.13885896963736144 1105 36-37 0.10411415097117782 1105 38-39 0.3347799241907765 1105 40-41 0.31091579986480866 1105 42-43 0.3258962517587207 1105 44-45 0.20048850996380452 1105 46-47 0.17539733517872946 1105 48-49 0.08533687262148959 1105 50-51 0.281619403822468 1105 52-53 0.20235971002774278 1105 54-55 0.25884376996318537 1105 56-57 0.323923918858398 1105 58-59 0.2966430877118569 1105 60-61 0.1889098892567489 1105 62-63 0.1929828153320372 1105 64-65 0.2763860097688209 1105 66-67 0.2559288525837786 1105 68-69 0.4581945471794384 1105 70-71 0.36791172637417446 1105 72-73 0.26065014711986834 1105 74-75 0.3078109119773451 1105 76-77 0.24115113805711985 1105 78-79 0.4617973019788728 1105 80-81 0.3642485419341348 1105 82-83 0.11083474049979358 1105 84-85 0.40742744468381886 1105 86-87 0.1898255508015403 1105 88-89 0.3672679484201389 1105 90-91 0.4561435832958942 1105 92-93 0.29933123093331915 1105 94-95 0.330267959360512 1105 96-97 0.4379651062782308 1105 98-99 0.21967448285598934 1105 100-101 0.4428852332740014 1106 1 0.20514384957032306 1106 2 0.3135439471235344 1106 3 0.37620350103824407 1106 4 0.2419714793320722 1106 5 0.3902565377584324 1106 6 0.31832092951838575 1106 7 0.22851724223055925 1106 8 0.32558962786690415 1106 9 0.2840043584763521 1106 10-11 0.4105316489185995 1106 12-13 0.45415441333602047 1106 14-15 0.569481125771091 1106 16-17 0.42711707736411597 1106 18-19 0.3531499447392932 1106 20-21 0.43991250636670287 1106 22-23 0.38763564451023313 1106 24-25 0.45927241768593774 1106 26-27 0.34741843115448034 1106 28-29 0.39176613875785904 1106 30-31 0.3821195399965802 1106 32-33 0.42965438186414673 1106 34-35 0.369811908207744 1106 36-37 0.28262123224069313 1106 38-39 0.291784427870617 1106 40-41 0.23918109400548104 1106 42-43 0.2518857166579238 1106 44-45 0.1866321504572852 1106 46-47 0.2738855027605709 1106 48-49 0.2531456625621331 1106 50-51 0.1520155054311445 1106 52-53 0.11378576167548715 1106 54-55 -0.015077057693730467 1106 56-57 0.20343596339268544 1106 58-59 0.07389022445983784 1106 60-61 0.0641227945027083 1106 62-63 0.1881088792269736 1106 64-65 0.1738960442503199 1106 66-67 0.09440621234408297 1106 68-69 0.03238247701445829 1106 70-71 0.12000037576263622 1106 72-73 0.06459487571854794 1106 74-75 0.43562823706677634 1106 76-77 0.36787347678889404 1106 78-79 0.47911096490867777 1106 80-81 0.35671463176278806 1106 82-83 0.2765772921304972 1106 84-85 0.26214620774379327 1106 86-87 0.3244463742918846 1106 88-89 0.4213231541664655 1106 90-91 0.34745679674152896 1106 92-93 0.17386770038670818 1106 94-95 0.24561342455768198 1106 96-97 0.2883354888069327 1106 98-99 0.3386466918560238 1106 100-101 0.40210496158859854 1107 1 0.36856523234801486 1107 2 0.3599565165721259 1107 3 0.35072816924140326 1107 4 0.26452737621328737 1107 5 0.3535983710440789 1107 6 0.3625350402776917 1107 7 0.24782953215544268 1107 8 0.36846711850490266 1107 9 0.5083892648249062 1107 10-11 0.36377934339824947 1107 12-13 0.5093554723189868 1107 14-15 0.5513726114600388 1107 16-17 0.6095047254975299 1107 18-19 0.5067095486897202 1107 20-21 0.6670618361700917 1107 22-23 0.5862691540262972 1107 24-25 0.6430191673188475 1107 26-27 0.4466661696536036 1107 28-29 0.5367751155878864 1107 30-31 0.5521636538058061 1107 32-33 0.6190711551732448 1107 34-35 0.6383348344971225 1107 36-37 0.5808961401928059 1107 38-39 0.6060604174442901 1107 40-41 0.7470080722881534 1107 42-43 0.7020173034659081 1107 44-45 0.7387757420067942 1107 46-47 0.6698148011843585 1107 48-49 0.7287771063068078 1107 50-51 0.7476094697313371 1107 52-53 0.8667790864354998 1107 54-55 1.0375070430983087 1107 56-57 1.0503566434307956 1107 58-59 0.9756433003211242 1107 60-61 0.969852087266851 1107 62-63 1.0087048086826087 1107 64-65 1.0758490943287669 1107 66-67 0.9976219926994503 1107 68-69 1.0447052592972454 1107 70-71 0.9645682145004102 1107 72-73 1.0575437408019823 1107 74-75 1.0389897533492665 1107 76-77 0.9290552112193637 1107 78-79 0.8513164352725617 1107 80-81 0.7998890966902685 1107 82-83 0.8934021669107963 1107 84-85 0.7596265640500555 1107 86-87 0.7854870891682673 1107 88-89 0.8535988670462515 1107 90-91 0.6186480896880369 1107 92-93 0.6829487622623773 1107 94-95 0.8238833645968597 1107 96-97 0.5569637474276767 1107 98-99 0.6859732944009806 1107 100-101 0.7340745818576835 1108 1 -0.0021491558973032454 1108 2 0.17497421517364842 1108 3 0.06931246436672822 1108 4 0.27171554334672976 1108 5 0.33449212751498436 1108 6 0.16087543370889534 1108 7 0.02769741110250834 1108 8 0.08453787626159937 1108 9 0.1541585508031531 1108 10-11 0.08581982875833916 1108 12-13 0.10824881343013004 1108 14-15 0.2625509809897366 1108 16-17 0.2882476208604672 1108 18-19 0.34448483905978833 1108 20-21 0.4369797109940947 1108 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-2.522084552366916 2316 82-83 -2.0395544398077092 2316 84-85 -1.937587885378349 2316 86-87 -2.174708082169108 2316 88-89 -2.295319288950207 2316 90-91 -2.2398934685891696 2316 92-93 -2.886526363235042 2316 94-95 -2.3763699152096542 2316 96-97 -2.4586035571549125 2316 98-99 -2.6428705474371164 2316 100-101 -2.7067688522016837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2776.0 3 980.0 4 998.0 5 1084.0 6 1296.0 7 1567.0 8 1967.0 9 2509.0 10 3211.0 11 4179.0 12 5818.0 13 7746.0 14 8930.0 15 11552.0 16 15217.0 17 20814.0 18 17788.0 19 10011.0 20 9626.0 21 9850.0 22 10559.0 23 11565.0 24 13437.0 25 15443.0 26 17789.0 27 20960.0 28 25031.0 29 30638.0 30 37293.0 31 46550.0 32 58878.0 33 77888.0 34 107213.0 35 154739.0 36 230503.0 37 340911.0 38 314789.0 39 54213.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00079547406327 20.02255699480802 15.129951855353857 21.846695675774853 2 14.792781144370664 21.236437977355944 40.93654517821777 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20.303996975562722 22-23 21.29658120710098 26.68359329897602 31.389056416442042 20.630769077480956 24-25 21.007450637257172 27.02879393212319 31.519717845134814 20.444037585484825 26-27 20.751903361790237 27.16253018918458 31.425234261039677 20.66033218798551 28-29 20.46238478987531 26.718551331855856 32.078589473209185 20.740474405059647 30-31 20.726192680874206 26.746092770189016 31.854325438779508 20.673389110157274 32-33 20.580139071625112 26.990854560851897 31.67208282175331 20.756923545769684 34-35 20.96393210314457 26.90243086964459 31.36152083705112 20.77211619015972 36-37 21.29988208570002 26.55741230856508 31.02614157686348 21.11656402887142 38-39 20.66517925690462 27.15612879632332 31.23305566006885 20.945636286703213 40-41 20.732153291024034 26.87126059954651 31.632492792982337 20.76409331644712 42-43 20.914521509396806 26.864182886720222 30.96352158206765 21.25777402181532 44-45 20.79558161287071 27.071587810990177 30.78870132518795 21.34412925095117 46-47 21.185777144317257 27.357055283756193 30.148318842142523 21.308848729784028 48-49 21.20765120034601 27.602991348928878 29.73992869452134 21.449428756203773 50-51 21.20964379200742 27.603929039122487 29.456160199681126 21.730266969188968 52-53 21.30690800510456 27.946674637047327 29.35154213679459 21.394875221053525 54-55 21.24567778233605 27.827052687100746 29.274893043427298 21.652376487135907 56-57 21.22991070251225 28.26971392805214 28.97345103699836 21.526924332437254 58-59 21.24684990916017 27.614809822422785 29.40552657797574 21.732813690441304 60-61 21.46793883734696 27.964155731507912 29.39860045596535 21.169304975179777 62-63 21.22420130716503 27.882192220773984 29.71766782629896 21.17593864576203 64-65 21.196306398281195 27.672123692054512 29.527012217063568 21.60455769260072 66-67 21.22082238027835 28.157395766479404 28.942147355588183 21.679634497654067 68-69 20.967105698900593 27.980260254228796 29.526036266317174 21.526597780553438 70-71 21.22425005539513 27.86996622362283 29.428445313571633 21.477338407410404 72-73 21.250286479291137 27.932038613012345 29.041233675076505 21.776441232620012 74-75 20.938748774579842 28.45354540245802 28.80374081026466 21.803965012697475 76-77 21.05644821374241 28.25321295393618 29.169922659305243 21.52041617301617 78-79 21.0291556186531 28.202189858678235 28.93047217747005 21.83818234519861 80-81 20.98462509018069 28.752432789918643 28.67231452093057 21.590627598970098 82-83 21.421923755058252 28.468989492907088 28.564460553333497 21.54462619870116 84-85 21.02748546478401 28.236126115089487 28.489814651450825 22.246573768675677 86-87 21.034413635332573 28.251031447801623 28.849971601849134 21.864583315016674 88-89 21.243420364318617 28.093919729397143 28.84474770626742 21.817912200016824 90-91 21.014314985965214 28.069430363737986 29.152059612899517 21.764195037397283 92-93 21.180555555555554 27.876263905303244 29.02195654416868 21.921223994972518 94-95 20.914086833384328 27.573854797851027 29.141477904810802 22.37058046395384 96-97 21.19985967170565 27.696754893538948 28.88816790391791 22.215217530837496 98-99 20.83764689054566 27.83915111538917 28.90995871881782 22.413243275247353 100-101 20.809729938567905 27.804053984731823 29.005522351614832 22.38069372508544 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 543.0 1 421.5 2 395.5 3 478.0 4 441.0 5 492.0 6 842.5 7 1810.5 8 3131.5 9 3669.0 10 3215.0 11 2828.0 12 2877.0 13 3031.0 14 3290.5 15 3449.0 16 3530.5 17 3705.5 18 3820.0 19 3995.0 20 4377.5 21 4957.0 22 5619.0 23 6639.0 24 7762.0 25 9290.5 26 12225.5 27 15004.0 28 16337.5 29 18688.5 30 23154.5 31 27514.5 32 33025.5 33 39954.0 34 46563.0 35 52044.5 36 60220.5 37 67905.0 38 67417.5 39 64433.0 40 61570.5 41 59306.0 42 57404.5 43 57707.0 44 59124.0 45 59089.0 46 55724.5 47 51227.5 48 52585.5 49 52654.5 50 49032.5 51 45910.5 52 44317.0 53 44478.5 54 44283.5 55 41364.0 56 37163.5 57 33354.0 58 30121.5 59 27469.0 60 24337.0 61 20622.5 62 16955.5 63 13376.0 64 10364.0 65 7954.0 66 5682.0 67 4546.0 68 3291.0 69 1822.5 70 1234.5 71 912.0 72 718.0 73 552.0 74 398.5 75 306.0 76 207.5 77 110.0 78 64.0 79 37.5 80 25.0 81 20.0 82 12.0 83 12.0 84 12.0 85 8.5 86 6.0 87 4.0 88 4.0 89 5.0 90 3.0 91 1.5 92 1.0 93 1.0 94 1.0 95 1.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024790133644020714 2 0.010607598556897753 3 0.011310864759565007 4 0.02133240814757338 5 0.052569148649377256 6 0.03879685218047686 7 0.01558906749245747 8 0.045770908690260466 9 0.0289511253431353 10-11 0.022270096417796387 12-13 0.03419631910469524 14-15 0.017610957825125825 16-17 0.01617512266134685 18-19 0.016849086105569636 20-21 0.012981121990899737 22-23 0.008028955813784487 24-25 0.0015823489560013223 26-27 5.860551688893785E-5 28-29 0.0023149179171130453 30-31 0.0 32-33 5.274496520004408E-4 34-35 2.9302758444468926E-5 36-37 0.0 38-39 1.172110337778757E-4 40-41 5.860551688893785E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.0511930911128252E-4 50-51 2.0511930911128252E-4 52-53 3.8093585977809605E-4 54-55 0.0014065324053345086 56-57 2.9302758444468927E-4 58-59 0.0014065324053345086 60-61 0.0031646979120026446 62-63 0.002109798608001763 64-65 0.0012893213715566329 66-67 0.010490387523119876 68-69 0.013069030266233144 70-71 0.02332499572179727 72-73 0.015647673009346406 74-75 0.016204425419791318 76-77 0.029009730860024237 78-79 0.018958884713571395 80-81 0.04222527491847973 82-83 0.03349305290202798 84-85 0.017288627482236668 86-87 0.013303452333788894 88-89 0.012160644754454605 90-91 0.022533821243796607 92-93 0.02930275844446893 94-95 0.012072736479121198 96-97 0.019017490230460333 98-99 0.027075748802689287 100-101 0.036306117712697004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1706324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.88132300728944 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41552890452033 33.0933318362483 2 13.235302473448723 11.615651658731606 3 4.402251994954103 5.795299252501959 4 2.076086411171063 3.6440567359856693 5 1.1343078416780779 2.488746439518853 6 0.6813047619278362 1.7937932594735888 7 0.46708227445391054 1.4347331709404025 8 0.33562141028617637 1.1782009210343776 9 0.2622864455142031 1.0358528612438314 >10 1.5975912250118498 13.844134392279333 >50 0.2264325911007921 6.9857651381817805 >100 0.15681374143582288 12.684524777599771 >500 0.00791436493325894 2.2673511048443737 >1k 0.0010731342282385005 0.586983702004416 >5k 2.682835570596251E-4 0.8393301852066752 >10k+ 1.3414177852981256E-4 0.7122445642051176 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12100 0.709126754356148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8404 0.49252076393463373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5855 0.34313530138473114 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1962 0.11498402413609608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 2.930275844446893E-5 0.0 0.0 0.0 0.0 16-17 5.860551688893786E-5 0.0 0.0 2.930275844446893E-5 0.0 18-19 5.860551688893786E-5 0.0 0.0 5.860551688893786E-5 0.0 20-21 5.860551688893786E-5 0.0 0.0 1.4651379222234466E-4 0.0 22-23 5.860551688893786E-5 0.0 0.0 4.10238618222565E-4 0.0 24-25 5.860551688893786E-5 0.0 0.0 7.325689611117233E-4 0.0 26-27 5.860551688893786E-5 0.0 0.0 9.962937871119434E-4 0.0 28-29 5.860551688893786E-5 0.0 0.0 0.0019046792988904803 0.0 30-31 5.860551688893786E-5 0.0 0.0 0.005714037896671441 0.0 32-33 5.860551688893786E-5 0.0 0.0 0.008966644084007491 0.0 34-35 5.860551688893786E-5 0.0 0.0 0.011222956484231598 0.0 36-37 5.860551688893786E-5 0.0 0.0 0.013918810261122742 0.0 38-39 5.860551688893786E-5 0.0 0.0 0.019544939882460775 0.0 40-41 5.860551688893786E-5 0.0 0.0 0.02698784052735588 0.0 42-43 5.860551688893786E-5 0.0 0.0 0.03355165841891693 0.0 44-45 5.860551688893786E-5 0.0 0.0 0.03733171425825342 0.0 46-47 5.860551688893786E-5 0.0 0.0 0.04163921974959035 2.930275844446893E-5 48-49 5.860551688893786E-5 0.0 0.0 0.04908212039448545 5.860551688893786E-5 50-51 5.860551688893786E-5 0.0 0.0 0.055704543802935436 5.860551688893786E-5 52-53 5.860551688893786E-5 0.0 0.0 0.07105918922783716 8.790827533340679E-5 54-55 5.860551688893786E-5 0.0 0.0 0.08178399881851278 1.4651379222234466E-4 56-57 5.860551688893786E-5 0.0 0.0 0.10956301382386932 1.7581655066681358E-4 58-59 5.860551688893786E-5 0.0 0.0 0.15952421697168884 1.7581655066681358E-4 60-61 5.860551688893786E-5 0.0 0.0 0.20213042774994666 1.7581655066681358E-4 62-63 5.860551688893786E-5 0.0 0.0 0.22469355175218775 2.3442206755575144E-4 64-65 5.860551688893786E-5 0.0 0.0 0.2681495425253352 2.3442206755575144E-4 66-67 5.860551688893786E-5 0.0 0.0 0.3550029185547411 2.637248260002204E-4 68-69 5.860551688893786E-5 0.0 0.0 0.4840522667441822 2.9302758444468927E-4 70-71 5.860551688893786E-5 0.0 0.0 0.5934980695342736 2.9302758444468927E-4 72-73 5.860551688893786E-5 2.930275844446893E-5 0.0 0.7201738942897129 2.9302758444468927E-4 74-75 5.860551688893786E-5 5.860551688893786E-5 0.0 0.874658036808953 2.9302758444468927E-4 76-77 5.860551688893786E-5 5.860551688893786E-5 0.0 0.9723534334628124 2.9302758444468927E-4 78-79 5.860551688893786E-5 5.860551688893786E-5 0.0 1.095425018929582 2.9302758444468927E-4 80-81 5.860551688893786E-5 5.860551688893786E-5 0.0 1.2714759916639513 2.9302758444468927E-4 82-83 5.860551688893786E-5 5.860551688893786E-5 0.0 1.4302090341576394 2.9302758444468927E-4 84-85 5.860551688893786E-5 5.860551688893786E-5 0.0 1.6430642714982617 2.9302758444468927E-4 86-87 5.860551688893786E-5 5.860551688893786E-5 0.0 1.9658634585225314 2.9302758444468927E-4 88-89 5.860551688893786E-5 5.860551688893786E-5 0.0 2.3668131023181997 2.9302758444468927E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4970 0.0 51.05689 1 GTATCAA 9640 0.0 44.265842 1 ATCAACG 12340 0.0 34.233875 3 TCAACGC 12420 0.0 34.013367 4 TATCAAC 12630 0.0 33.52307 2 CAACGCA 12685 0.0 33.37772 5 AACGCAG 13005 0.0 32.664135 6 ACGCAGA 14660 0.0 28.716469 7 CGCAGAG 14685 0.0 28.602867 8 GCAGAGT 15180 0.0 26.888426 9 GTACATG 9605 0.0 26.715658 1 ACATGGG 9680 0.0 25.330503 3 TACATGG 10090 0.0 25.290216 2 CATGGGG 2875 0.0 24.627361 4 GAGTACT 8655 0.0 23.962696 12-13 AGTACTT 9380 0.0 22.363842 12-13 GTACTTT 9220 0.0 22.286175 14-15 AGAGTAC 14275 0.0 22.019007 10-11 TACGCCG 110 0.0028711776 21.599653 5 CATGGGA 6650 0.0 21.365793 4 >>END_MODULE